Heat-induced temperature sensitivity of outgrowing Bacillus cereus spores.

abstract：:The prophage-encoded Shiga toxin is a major virulence factor in Stx-producing Escherichia coli (STEC). Toxin production and phage production are linked and occur after induction of the RecA-dependent SOS response. However, food-related stress and Stx-prophage induction have not been studied at the single-cell level. T...

journal_title：Applied and environmental microbiology

authors： Fang Y,Mercer RG,McMullen LM,Gänzle MG

更新日期：2017-09-15 00:00:00

abstract：:GELRITE, a new gelling agent with good thermal stability and clarity, was evaluated in media for culturing selected thermophilic microorganisms. It was also evaluated for performing counts of thermophilic bacteria from soil samples. In most cases, GELRITE was shown to be superior to agar for these applications. ...

journal_title：Applied and environmental microbiology

authors： Lin CC,Casida LE

更新日期：1984-02-01 00:00:00

abstract：:Two strains of 1,1,1-trichloroethane (TCA)-degrading bacteria, TA5 and TA27, were isolated from soil and identified as Mycobacterium spp. Strains TA5 and TA27 could degrade 25 and 75 mg. liter of TCA(-1) cometabolically in the presence of ethane as a carbon source, respectively. The compound 2,2,2-trichloroethanol was...

journal_title：Applied and environmental microbiology

authors： Yagi O,Hashimoto A,Iwasaki K,Nakajima M

更新日期：1999-10-01 00:00:00

abstract：:Intergeneric conjugal transfer of plasmid DNA from Escherichia coli to Streptomyces circumvents problems such as host-controlled restriction and instability of foreign DNA during the transformation of Streptomyces protoplasts. The anthracycline antibiotic-producing strains Streptomyces peucetius and Streptomyces sp. s...

journal_title：Applied and environmental microbiology

authors： Paranthaman S,Dharmalingam K

更新日期：2003-01-01 00:00:00

abstract：:The sediment-water interface in Halifax Harbor supports a microbial population of 6.95 x 10 cells per g (dry weight). As determined by the standard technique of suspending subsamples in filtered seawater, the uptake of added glutamic acid by this population is 113.5 ng g (dry weight) h. An alternate technique was deve...

journal_title：Applied and environmental microbiology

authors： Novitsky JA

更新日期：1983-06-01 00:00:00

abstract：:Fluorescence in situ hybridization (FISH) and rRNA slot blot hybridization with 16S rRNA-targeted oligonucleotide probes were used to investigate the phylogenetic composition of a marine Arctic sediment (Svalbard). FISH resulted in the detection of a large fraction of microbes living in the top 5 cm of the sediment. U...

journal_title：Applied and environmental microbiology

authors： Ravenschlag K,Sahm K,Amann R

更新日期：2001-01-01 00:00:00

abstract：:Penicillium expansum isolated from meat and apples produced both patulin and citrinin. Toxin identity was confirmed by spectroscopic and physical methods. The mean lethal dose in chicken embryos was determined for toxins administered both singly and in various ratios. Data from simultaneous administration of mycotoxin...

journal_title：Applied and environmental microbiology

authors： Ciegler A,Vesonder RF,Jackson LK

更新日期：1977-04-01 00:00:00

abstract：:A mixed culture of nitrate-reducing bacteria degraded o-cresol in the presence of toulene as a primary growth substrate. No degradation of o-cresol was observed in the absence of toluene or when the culture grew on p-cresol and 2,4-dimethylphenol. In batch cultures, the degradation of o-cresol started after toluene wa...

journal_title：Applied and environmental microbiology

authors： Flyvbjerg J,Jørgensen C,Arvin E,Jensen BK,Olsen SK

更新日期：1993-07-01 00:00:00

abstract：:Preliminary observations in a large-scale fermentation process suggested that the lipase expression of Pseudomonas alcaligenes can be switched on by the addition of certain medium components, such as soybean oil. In an attempt to elucidate the mechanism of induction of lipase expression, we have set up a search method...

journal_title：Applied and environmental microbiology

authors： Krzeslak J,Gerritse G,van Merkerk R,Cool RH,Quax WJ

更新日期：2008-03-01 00:00:00

abstract：:Polyhydroxybutyrate (PHB) is a carbon and energy reserve polymer in various prokaryotic species. We determined that, when grown with mannitol as the sole carbon source, Bradyrhizobium diazoefficiens produces a homopolymer composed only of 3-hydroxybutyrate units (PHB). Conditions of oxygen limitation (such as microoxi...

journal_title：Applied and environmental microbiology

authors： Quelas JI,Mesa S,Mongiardini EJ,Jendrossek D,Lodeiro AR

更新日期：2016-06-30 00:00:00

abstract：:A bacterial strain capable of growing on propachlor (2-chloro-N-isopropylacetanilide) was isolated from soil by using enrichment and isolation techniques. The strain isolated, designated GCH1, was classified as a member of the genus Pseudomonas. Washed-cell suspensions of strain GCH1 accumulated N-isopropylacetanilide...

journal_title：Applied and environmental microbiology

authors： Martín M,Mengs G,Plaza E,Garbi C,Sánchez M,Gibello A,Gutierrez F,Ferrer E

更新日期：2000-03-01 00:00:00

abstract：:Because Bacteroides spp. are obligate anaerobes that dominate the human fecal flora, and because some species may live only in the human intestine, these bacteria might be useful to distinguish human from nonhuman sources of fecal pollution. To test this hypothesis, PCR primers specific for 16S rRNA gene sequences of ...

journal_title：Applied and environmental microbiology

authors： Kreader CA

更新日期：1995-04-01 00:00:00

abstract：:Pseudomonas sp. strain KIE171 was able to grow with isopropylamine or L-alaninol [S-(+)-2-amino-1-propanol] as the sole carbon source, but not with D-alaninol. To investigate the hypothesis that L-alaninol is an intermediate in the degradation of isopropylamine, two mini-Tn5 mutants unable to utilize both isopropylami...

journal_title：Applied and environmental microbiology

authors： de Azevedo Wäsch SI,van der Ploeg JR,Maire T,Lebreton A,Kiener A,Leisinger T

更新日期：2002-05-01 00:00:00

abstract：:Some nitrate- and Fe(III)-reducing microorganisms are capable of oxidizing Fe(II) with nitrate as the electron acceptor. This enzymatic pathway may facilitate the development of anaerobic microbial communities that take advantage of the energy available during Fe-N redox oscillations. We examined this phenomenon in sy...

journal_title：Applied and environmental microbiology

authors： Coby AJ,Picardal F,Shelobolina E,Xu H,Roden EE

更新日期：2011-09-01 00:00:00

abstract：:Achromobacter spp. are nonfermentative Gram-negative bacilli considered emergent pathogens in cystic fibrosis (CF). Although some cross-transmission events between CF patients have been described, Achromobacter strains were mostly patient specific, suggesting sporadic acquisitions from nonhuman reservoirs. However, so...

journal_title：Applied and environmental microbiology

authors： Dupont C,Jumas-Bilak E,Doisy C,Aujoulat F,Chiron R,Marchandin H

更新日期：2018-11-15 00:00:00

abstract：UNLABELLED:The efficiency of direct steam injection (DSI) at 105 °C for 3 s to inactivate Mycobacterium avium subsp. paratuberculosis in milk at a pilot-plant scale was investigated. Milk samples were artificially contaminated with M. avium subsp. paratuberculosis and also with cow fecal material naturally infected wit...

journal_title：Applied and environmental microbiology

authors： Peterz M,Butot S,Jagadeesan B,Bakker D,Donaghy J

更新日期：2016-04-18 00:00:00

abstract：:An extracellular serine proteinase from Brevibacterium linens ATCC 9174 was purified to homogeneity. pH and temperature optima were 8.5 and 50(deg)C, respectively. The results for the molecular mass of the proteinase were 56 kDa by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and 126 kDa by gel filtration...

journal_title：Applied and environmental microbiology

authors： Rattray FP,Bockelmann W,Fox PF

更新日期：1995-09-01 00:00:00

abstract：:A field study was conducted to determine the microbial community structures of streambed sediments across diverse geographic and climatic areas. Sediment samples were collected from three adjacent headwater forest streams within three biomes, eastern deciduous (Pennsylvania), southeastern coniferous (New Jersey), and ...

journal_title：Applied and environmental microbiology

authors： Findlay RH,Yeates C,Hullar MA,Stahl DA,Kaplan LA

更新日期：2008-05-01 00:00:00

abstract：:To determine whether the pathogenic variant of Lyme disease spirochetes, isolate A14S, is perpetuated in a particular reservoir-vector relationship, we screened vector ticks in various Central European sites for a related spirochete and determined its host association. A14S-like spirochetes infect numerous questing ti...

journal_title：Applied and environmental microbiology

authors： Richter D,Schlee DB,Allgöwer R,Matuschka FR

更新日期：2004-11-01 00:00:00

abstract：:Vibrio vulnificus biotype 2 is a primary pathogen for eels and, as has recently been suggested, an opportunistic pathogen for humans. In this study we have investigated the ability of V. vulnificus biotype 2 to obtain iron by siderophore-mediated mechanisms and evaluated the importance of free iron in vibriosis. The v...

journal_title：Applied and environmental microbiology

authors： Biosca EG,Fouz B,Alcaide E,Amaro C

更新日期：1996-03-01 00:00:00

abstract：:We isolated a strain of Rhodopseudomonas palustris (RCB100) by selective enrichment in light on 3-chlorobenzoate to investigate the steps that it uses to accomplish anaerobic dechlorination. Analyses of metabolite pools as well as enzyme assays suggest that R. palustris grows on 3-chlorobenzoate by (i) converting it t...

journal_title：Applied and environmental microbiology

authors： Egland PG,Gibson J,Harwood CS

更新日期：2001-03-01 00:00:00

abstract：:A census of clean room surface-associated bacterial populations was derived from the results of both the cloning and sequencing of 16S rRNA genes and DNA microarray (PhyloChip) analyses. Samples from the Lockheed Martin Aeronautics Multiple Testing Facility (LMA-MTF), the Kennedy Space Center Payload Hazard and Servic...

journal_title：Applied and environmental microbiology

authors： La Duc MT,Osman S,Vaishampayan P,Piceno Y,Andersen G,Spry JA,Venkateswaran K

更新日期：2009-10-01 00:00:00

abstract：:Fluctuations in ciliated protozoan concentrations were monitored in 40 individually fed crossbred heifers that were stepped up to an 85% concentrate diet either slowly (12 days) or rapidly (3 days), with or without monensin (30 ppm). Ruminal fluid was withdrawn from all animals by stomach tube at the start of the stud...

journal_title：Applied and environmental microbiology

authors： Towne G,Nagaraja TG,Brandt RT Jr,Kemp KE

更新日期：1990-10-01 00:00:00

abstract：:Although biofilms represent a common bacterial lifestyle in clinically and environmentally important habitats, there is scant information on the extent of gene transfer in these spatially structured populations. The objective of this study was to gain insight into factors that affect transfer of the promiscuous multid...

journal_title：Applied and environmental microbiology

authors： Król JE,Nguyen HD,Rogers LM,Beyenal H,Krone SM,Top EM

更新日期：2011-08-01 00:00:00

abstract：:In this study, we used chemostat cultures to analyze the quantitative effects of the specific growth rate and respiration on the metabolism in Lactococcus lactis CHCC2862 and on the downstream robustness of cells after freezing or freeze-drying. Under anaerobic conditions, metabolism remained homofermentative, althoug...

journal_title：Applied and environmental microbiology

authors： Johanson A,Goel A,Olsson L,Franzén CJ

更新日期：2020-03-02 00:00:00

abstract：:The abundance of quorum quenching (QQ) activity was evaluated in cultivable bacteria obtained from oceanic and estuarine seawater and compared with the frequency of QQ enzyme sequences in the available marine metagenomic collections. The possible role of the high QQ activity found among marine bacteria is discussed. ...

journal_title：Applied and environmental microbiology

authors： Romero M,Martin-Cuadrado AB,Otero A

更新日期：2012-09-01 00:00:00

abstract：:By taking advantage of MazF, an ACA codon-specific mRNA interferase, Escherichia coli cells can be converted into a bioreactor producing only a single protein of interest by using an ACA-less mRNA for the protein. In this single-protein production (SPP) system, we engineered MazF by replacing two tryptophan residues i...

journal_title：Applied and environmental microbiology

authors： Vaiphei ST,Mao L,Shimazu T,Park JH,Inouye M

更新日期：2010-09-01 00:00:00

abstract：:To examine whether the heat-labile enterotoxin gene in porcine enterotoxigenic Escherichia coli (ETEC) strains is as divergent as in human ETEC strains, we sequenced the heat-labile and heat-stable toxin genes from 52 and 33 porcine ETEC strains, respectively. We found that the STa gene is identical, that the LT gene ...

journal_title：Applied and environmental microbiology

authors： Zhang C,Rausch D,Zhang W

更新日期：2009-10-01 00:00:00

abstract：:Recent physiological work has shown that the filamentous euendolithic cyanobacterium Mastigocoleus testarum (strain BC008) is able to bore into solid carbonates using Ca²⁺-ATPases to take up Ca²⁺ from the medium at the excavation front, promoting dissolution of CaCO₃ there. It is not known, however, if this is a wides...

journal_title：Applied and environmental microbiology

authors： Ramírez-Reinat EL,Garcia-Pichel F

更新日期：2012-01-01 00:00:00

abstract：:The Cry2Aa3 gene was introduced into asporogenic Bacillus thuringiensis, and the synthesized protoxin killed Bombyx mori and Lymantria dispar larvae. Chymotrypsin hydrolyzed the linkages between 49Tyr/Val50 and 145Lys/Ser146 in the protoxin, and 50- and 58-kDa fragments were generated, respectively. Both peptides kill...

journal_title：Applied and environmental microbiology

authors： Ohsawa M,Tanaka M,Moriyama K,Shimazu M,Asano S,Miyamoto K,Haginoya K,Mitsui T,Kouya T,Taniguchi M,Hori H

更新日期：2012-07-01 00:00:00