Abstract:
:Vibrio vulnificus biotype 2 is a primary pathogen for eels and, as has recently been suggested, an opportunistic pathogen for humans. In this study we have investigated the ability of V. vulnificus biotype 2 to obtain iron by siderophore-mediated mechanisms and evaluated the importance of free iron in vibriosis. The virulence degree for eels was dependent on iron availability from host fluids, as was revealed by a reduction in the 50% lethal dose for iron-overloaded eels. This biotype produced both phenolate- and hydroxamate-type siderophores of an unknown nature and two new outer membrane proteins of around 84 and 72 kDa in response to iron starvation. No alterations in lipopolysaccharide patterns were detected in response to iron stress. Finally, our data suggest that V. vulnificus biotype 2 uses the hydroxamate-type siderophore for removal of iron from transferrin rather than relying on a receptor for this iron-binding protein.
journal_name
Appl Environ Microbioljournal_title
Applied and environmental microbiologyauthors
Biosca EG,Fouz B,Alcaide E,Amaro Cdoi
10.1128/AEM.62.3.928-935.1996subject
Has Abstractpub_date
1996-03-01 00:00:00pages
928-35issue
3eissn
0099-2240issn
1098-5336journal_volume
62pub_type
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