An aflatoxin biosynthesis cluster gene encodes a novel oxidase required for conversion of versicolorin a to sterigmatocystin.

abstract：:Bacteria were isolated from lake water, and their ability to remain viable in a dilute, nutrient-deficient environment was tested by a method that permits suspension of test bacteria between two appressed microporous membranes in an aqueous environment. This approach permitted separation of the lake isolates into two ...

journal_title：Applied and environmental microbiology

authors： Sjogren RE,Gibson MJ

更新日期：1981-06-01 00:00:00

abstract：:Surfaces made of copper or its alloys have strong antimicrobial properties against a wide variety of microorganisms. However, the molecular mode of action responsible for the antimicrobial efficacy of metallic copper is not known. Here, we show that dry copper surfaces inactivate Candida albicans and Saccharomyces cer...

journal_title：Applied and environmental microbiology

authors： Quaranta D,Krans T,Espírito Santo C,Elowsky CG,Domaille DW,Chang CJ,Grass G

更新日期：2011-01-01 00:00:00

abstract：:Conditions for detection and quantitation of Clostridium perfringens enterotoxin by counterimmunoelectrophoresis are described. As little as 0.2 microgram of enterotoxin per ml could be detected. The test was found to be rapid, sensitive, specific and easy for the detection and quantitation of enterotoxin. ...

journal_title：Applied and environmental microbiology

authors： Naik HS,Duncan CL

更新日期：1977-08-01 00:00:00

abstract：:Certain strains of Enterococcus faecium and Enterococcus faecalis contribute beneficially to animal health and food production, while others are associated with nosocomial infections. To determine whether there are structural and functional genomic features that are distinct between nonclinical (NC) and clinical (CL) ...

journal_title：Applied and environmental microbiology

authors： Kim EB,Marco ML

更新日期：2014-01-01 00:00:00

abstract：:The diversities leaf-associated bacteria on nonnodulated (Nod(-)), wild-type nodulated (Nod(+)), and hypernodulated (Nod(++)) soybeans were evaluated by clone library analyses of the 16S rRNA gene. To analyze the impact of nitrogen fertilization on the bacterial leaf community, soybeans were treated with standard nitr...

journal_title：Applied and environmental microbiology

authors： Ikeda S,Anda M,Inaba S,Eda S,Sato S,Sasaki K,Tabata S,Mitsui H,Sato T,Shinano T,Minamisawa K

更新日期：2011-03-01 00:00:00

abstract：:Analysis of a complete set of glutamate decarboxylase (gad) mutants of Listeria monocytogenes strain LO28 (ΔgadD1, ΔgadDT1, ΔgadD2, ΔgadT2, and ΔgadD3 mutants) revealed that the ΔgadD1 mutant is impaired in its ability to tolerate exposure to both sublethal and lethal levels of the lantibiotic nisin. gadD1 is strain v...

journal_title：Applied and environmental microbiology

authors： Begley M,Cotter PD,Hill C,Ross RP

更新日期：2010-10-01 00:00:00

abstract：:The possible existence of endemism among microorganisms resulting from and preserved by geographic isolation is one of the most controversial topics in microbial ecology. We isolated 31 strains of "Spumella-like" flagellates from remote sampling sites from all continents, including Antarctica. These and another 23 iso...

journal_title：Applied and environmental microbiology

authors： Boenigk J,Pfandl K,Garstecki T,Harms H,Novarino G,Chatzinotas A

更新日期：2006-08-01 00:00:00

abstract：:An experiment was conducted with subsurface sediments from Oak Ridge National Laboratory to determine the potential for reduction of U(VI) under sulfate-reducing conditions with either ethanol or acetate as the electron donor. The results showed extensive U(VI) reduction in sediments supplied with either electron dono...

journal_title：Applied and environmental microbiology

authors： Converse BJ,Wu T,Findlay RH,Roden EE

更新日期：2013-07-01 00:00:00

abstract：:Rsan-ver, a strain of Pseudomonas aeruginosa isolated at this department, was used for the development of a continuous process for biosurfactant production. The active compounds were identified as rhamnolipids. A final medium for production was designed in continuous culture by means of medium shifts, since the format...

journal_title：Applied and environmental microbiology

authors： Guerra-Santos L,Käppeli O,Fiechter A

更新日期：1984-08-01 00:00:00

abstract：:The need for protozoa for the proliferation of Legionella pneumophila in aquatic habitats is still not fully understood and is even questioned by some investigators. This study shows the in vivo growth of L. pneumophila in protozoa in aquatic biofilms developing at high concentrations on plasticized polyvinyl chloride...

journal_title：Applied and environmental microbiology

authors： Kuiper MW,Wullings BA,Akkermans AD,Beumer RR,van der Kooij D

更新日期：2004-11-01 00:00:00

abstract：:An aminopeptidase was purified from cell extracts of Lactococcus lactis subsp. cremoris AM2 by ion-exchange chromatography. After electrophoresis of the purified enzyme in the presence or absence of sodium dodecyl sulfate, one protein band was detected. The enzyme was a 300-kilodalton hexamer composed of identical sub...

journal_title：Applied and environmental microbiology

authors： Neviani E,Boquien CY,Monnet V,Thanh LP,Gripon JC

更新日期：1989-09-01 00:00:00

abstract：:Addition of 2 mg of Pb2+/g of soil concident with or after amendment with starch or maltose resulted in 75 and 50% decreases in net synthesis of amylase and alpha-glucosidase, respectively. Invertase synthesis in sucrose-amended soil was transiently reduced after Pb2+ addition. Amylase activity was several times less ...

journal_title：Applied and environmental microbiology

authors： Cole MA

更新日期：1977-02-01 00:00:00

abstract：:Obligate aerobic organisms rely on a functional electron transport chain for energy conservation and NADH oxidation. Because of this essential requirement, the genes of this pathway are likely constitutively and highly expressed to avoid a cofactor imbalance and energy shortage under fluctuating environmental conditio...

journal_title：Applied and environmental microbiology

authors： Nies SC,Dinger R,Chen Y,Wordofa GG,Kristensen M,Schneider K,Büchs J,Petzold CJ,Keasling JD,Blank LM,Ebert BE

更新日期：2020-05-19 00:00:00

abstract：:Here we describe a quantitative PCR-based approach to estimating the relative abundances of major taxonomic groups of bacteria and fungi in soil. Primers were thoroughly tested for specificity, and the method was applied to three distinct soils. The technique provides a rapid and robust index of microbial community st...

journal_title：Applied and environmental microbiology

authors： Fierer N,Jackson JA,Vilgalys R,Jackson RB

更新日期：2005-07-01 00:00:00

abstract：:Preinfection development in Colletotrichum spp. exhibits three morphologies (conidia, germ tubes, and appressoria) and is directed by a complex interplay of environmental signals. Germ tube morphogenesis for Colletotrichum trifolii and the related fungus Colletotrichum gloeosporioides f. sp. aeschynomene was shown to ...

journal_title：Applied and environmental microbiology

authors： Buhr TL,Dickman MB

更新日期：1997-06-01 00:00:00

abstract：:Vibrio cholerae O1 in plankton samples collected from ponds and rivers between February 1987 and January 1990 in Matlab, Bangladesh, was detected by the fluorescent-monoclonal antibody (FA) technique. Samples were collected at sites which were monitored fortnightly (fixed sites) as well as at sites that were part of a...

journal_title：Applied and environmental microbiology

authors： Huq A,Colwell RR,Rahman R,Ali A,Chowdhury MA,Parveen S,Sack DA,Russek-Cohen E

更新日期：1990-08-01 00:00:00

abstract：:A triplex reverse transcriptase PCR (RT-PCR) was developed to simultaneously detect poliovirus, hepatitis A virus (HAV), and rotavirus in sewage and ocean water. Sewage and ocean water samples seeded with the three different viruses were concentrated by ultrafiltration. The unseeded ocean water and sewage samples were...

journal_title：Applied and environmental microbiology

authors： Tsai YL,Tran B,Sangermano LR,Palmer CJ

更新日期：1994-07-01 00:00:00

abstract：:Noroviruses (NoV) in 78 wastewater samples from Luxembourg were quantified, cloned, and sequenced in 2008-2009. The concentrations of NoV genogroup II and the relative occurrences of certain genotypes changed significantly during the winter season. NoV genogroup I was frequently detected by real-time reverse transcrip...

journal_title：Applied and environmental microbiology

authors： Skraber S,Langlet J,Kremer JR,Mossong J,De Landtsheer S,Even J,Muller CP,Hoffmann L,Cauchie HM

更新日期：2011-08-01 00:00:00

abstract：:Phylogenetic relationships, diversity, and in situ identification of spirochetes in the gut of the termite Neotermes koshunensis were examined without cultivation, with an emphasis on ectosymbionts attached to flagellated protists. Spirochetes in the gut microbial community investigated so far are related to the genus...

journal_title：Applied and environmental microbiology

authors： Noda S,Ohkuma M,Yamada A,Hongoh Y,Kudo T

更新日期：2003-01-01 00:00:00

abstract：:The PCR technique has potential for use in detection of low concentrations of airborne microorganisms. In this study, the sensitivity of PCR and its susceptibility to environmental interference were assessed with Escherichia coli DH1 as the target organism. Air samples, containing environmental bioaerosols, were colle...

journal_title：Applied and environmental microbiology

authors： Alvarez AJ,Buttner MP,Stetzenbach LD

更新日期：1995-10-01 00:00:00

abstract：:For simultaneous identification of members of the betaproteobacterial order "Rhodocyclales" in environmental samples, a 16S rRNA gene-targeted oligonucleotide microarray (RHC-PhyloChip) consisting of 79 probes was developed. Probe design was based on phylogenetic analysis of available 16S rRNA sequences from all cultu...

journal_title：Applied and environmental microbiology

authors： Loy A,Schulz C,Lücker S,Schöpfer-Wendels A,Stoecker K,Baranyi C,Lehner A,Wagner M

更新日期：2005-03-01 00:00:00

abstract：:A human feeding study was performed with Lactococcus lactis TC165.5, which is genetically marked by insertion of the sucrose-nisin conjugative transposon Tn5276 and chromosomal resistance to rifampin and streptomycin. The fate of strain TC165.5 and its nucleic acids was monitored by conventional plating methods and by...

journal_title：Applied and environmental microbiology

authors： Klijn N,Weerkamp AH,de Vos WM

更新日期：1995-07-01 00:00:00

abstract：:Vibrio cholerae can shift to a "rugose" phenotype, thereby producing copious exopolysaccharide (EPS), which promotes its environmental survival and persistence. We report conditions that promote high-frequency rugose EPS production (HFRP), whereby cells switch at high frequency (up to 80%) to rugose EPS production. HF...

journal_title：Applied and environmental microbiology

authors： Ali A,Rashid MH,Karaolis DK

更新日期：2002-11-01 00:00:00

abstract：:Cryptosporidium is a genus of waterborne protozoan parasites that causes significant gastrointestinal disease in humans. These parasites can accumulate in environmental biofilms and be subsequently released to contaminate water supplies. Natural microbial assemblages were collected each season from an eastern Pennsylv...

journal_title：Applied and environmental microbiology

authors： Wolyniak EA,Hargreaves BR,Jellison KL

更新日期：2010-02-01 00:00:00

abstract：:To assess the relative merits of tryptone yeast extract agar, the same medium unbuffered, and medium M17 for the assay of nine bacteriophages of lactic streptococci, comparative plaque counts were made with an overlay of 3 or 9 ml. Four of the phages exhibited no significant difference in plating efficiency between me...

journal_title：Applied and environmental microbiology

authors： Keogh BP

更新日期：1980-10-01 00:00:00

abstract：:NAD+-dependent formate dehydrogenase (FDH; EC 1.2.1.2) is an industrial enzyme widely used for NADH regeneration. However, enzyme inactivation caused by the oxidation of cysteine residues is a flaw of native FDH. In this study, we relieved the oxidation of the free cysteine of FDH from Candida boidinii (CboFDH) throug...

journal_title：Applied and environmental microbiology

authors： Zheng J,Yang T,Zhou J,Xu M,Zhang X,Rao Z

更新日期：2016-12-30 00:00:00

abstract：:The human intestine harbors both lactate-producing and lactate-utilizing bacteria. Lactate is normally present at <3 mmol liter(-1) in stool samples from healthy adults, but concentrations up to 100 mmol liter(-1) have been reported in gut disorders such as ulcerative colitis. The effect of different initial pH values...

journal_title：Applied and environmental microbiology

authors： Belenguer A,Duncan SH,Holtrop G,Anderson SE,Lobley GE,Flint HJ

更新日期：2007-10-01 00:00:00

abstract：:The synergistic antimicrobial effects of phytic acid (PA), a natural extract from rice bran, plus sodium chloride against Escherichia coli O157:H7 were examined. Exposure to NaCl alone at concentrations up to 36% (wt/wt) for 5 min did not reduce bacterial populations. The bactericidal effects of PA alone were much gre...

journal_title：Applied and environmental microbiology

authors： Kim NH,Rhee MS

更新日期：2015-12-04 00:00:00

abstract：:Spores of Clostridium bifermentans KMR-1 were produced for use as a microbial inoculum for bioremediation and were preserved in both liquid and dry forms. All spore formulations showed good viability and ability to biodegrade the target compound, 2,4,6-trinitrotoluene (TNT), after 4 months of storage. For low-cost bul...

journal_title：Applied and environmental microbiology

authors： Sembries S,Crawford RL

更新日期：1997-05-01 00:00:00

abstract：:An assembly consisting of Styrofoam cups with membranes of varying porosities was developed to study microorganism-root interactions. The assembly permitted uniform distribution of a bacterium in soil and was simple, easy to use, and disposable. In tests with the bacterium Pseudomonas solanacearum, little difference i...

journal_title：Applied and environmental microbiology

authors： Hartel PG,Billingsley JW,Williamson JW

更新日期：1989-05-01 00:00:00