Bacterial colonization of pellet softening reactors used during drinking water treatment.

Abstract:

:Pellet softening reactors are used in centralized and decentralized drinking water treatment plants for the removal of calcium (hardness) through chemically induced precipitation of calcite. This is accomplished in fluidized pellet reactors, where a strong base is added to the influent to increase the pH and facilitate the process of precipitation on an added seeding material. Here we describe for the first time the opportunistic bacterial colonization of the calcite pellets in a full-scale pellet softening reactor and the functional contribution of these colonizing bacteria to the overall drinking water treatment process. ATP analysis, advanced microscopy, and community fingerprinting with denaturing gradient gel electrophoretic (DGGE) analysis were used to characterize the biomass on the pellets, while assimilable organic carbon (AOC), dissolved organic carbon, and flow cytometric analysis were used to characterize the impact of the biological processes on drinking water quality. The data revealed pellet colonization at concentrations in excess of 500 ng of ATP/g of pellet and reactor biomass concentrations as high as 220 mg of ATP/m(3) of reactor, comprising a wide variety of different microorganisms. These organisms removed as much as 60% of AOC from the water during treatment, thus contributing toward the biological stabilization of the drinking water. Notably, only a small fraction (about 60,000 cells/ml) of the bacteria in the reactors was released into the effluent under normal conditions, while the majority of the bacteria colonizing the pellets were captured in the calcite structures of the pellets and were removed as a reusable product.

journal_name

Appl Environ Microbiol

authors

Hammes F,Boon N,Vital M,Ross P,Magic-Knezev A,Dignum M

doi

10.1128/AEM.02068-10

subject

Has Abstract

pub_date

2011-02-01 00:00:00

pages

1041-8

issue

3

eissn

0099-2240

issn

1098-5336

pii

AEM.02068-10

journal_volume

77

pub_type

杂志文章
  • Factors and selenocysteine insertion sequence requirements for the synthesis of selenoproteins from a gram-positive anaerobe in Escherichia coli.

    abstract::Selenoprotein synthesis in Escherichia coli strictly depends on the presence of a specific selenocysteine insertion sequence (SECIS) following the selenocysteine-encoding UGA codon of the respective mRNA. It is recognized by the selenocysteine-specific elongation factor SelB, leading to cotranslational insertion of se...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.02238-07

    authors: Gursinsky T,Gröbe D,Schierhorn A,Jäger J,Andreesen JR,Söhling B

    更新日期:2008-03-01 00:00:00

  • Influence of plant phenolic acids on growth and cellulolytic activity of rumen bacteria.

    abstract::Isolated rumen bacteria were examined for growth and, where appropriate, for their ability to degrade cellulose in the presence of the hydroxycinnamic acids trans-p-coumaric acid and trans-ferulic acid and the hydroxybenzoic acids vanillic acid and 4-hydroxybenzoic acid. Ferulic and p-coumaric acids proved to be the m...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.44.3.597-603.1982

    authors: Chesson A,Stewart CS,Wallace RJ

    更新日期:1982-09-01 00:00:00

  • Compatible solutes of the hyperthermophile Palaeococcus ferrophilus: osmoadaptation and thermoadaptation in the order thermococcales.

    abstract::The effect of salinity and growth temperature on the accumulation of intracellular organic solutes was examined in the hyperthermophilic archaeon Palaeococcus ferrophilus. The genus Palaeococcus represents a deep-branching lineage of the order Thermococcales, which diverged before Thermococcus and Pyrococcus. Palaeoco...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.71.12.8091-8098.2005

    authors: Neves C,da Costa MS,Santos H

    更新日期:2005-12-01 00:00:00

  • Inc A/C plasmids are prevalent in multidrug-resistant Salmonella enterica isolates.

    abstract::Bacterial plasmids are fragments of extrachromosomal double-stranded DNA that can contain a variety of genes that are beneficial to the host organism, like those responsible for antimicrobial resistance. The objective of this study was to characterize a collection of 437 Salmonella enterica isolates from different ani...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.02228-08

    authors: Lindsey RL,Fedorka-Cray PJ,Frye JG,Meinersmann RJ

    更新日期:2009-04-01 00:00:00

  • Variability of total and pathogenic Vibrio parahaemolyticus densities in northern Gulf of Mexico water and oysters.

    abstract::Vibrio parahaemolyticus is indigenous to coastal environments and a frequent cause of seafood-borne gastroenteritis in the United States, primarily due to raw-oyster consumption. Previous seasonal-cycle studies of V. parahaemolyticus have identified water temperature as the strongest environmental predictor. Salinity ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.01700-07

    authors: Zimmerman AM,DePaola A,Bowers JC,Krantz JA,Nordstrom JL,Johnson CN,Grimes DJ

    更新日期:2007-12-01 00:00:00

  • High-rate anaerobic treatment of wastewater at low temperatures.

    abstract::Anaerobic treatment of a volatile fatty acid (VFA) mixture was investigated under psychrophilic (3 to 8 degrees C) conditions in two laboratory-scale expanded granular sludge bed reactor stages in series. The reactor system was seeded with mesophilic methanogenic granular sludge and fed with a mixture of VFAs. Good re...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.65.4.1696-1702.1999

    authors: Lettinga G,Rebac S,Parshina S,Nozhevnikova A,van Lier JB,Stams AJ

    更新日期:1999-04-01 00:00:00

  • Production of penicillic acid and patulin by an atypical Penicillium roqueforti isolate.

    abstract::Simultaneous production of penicillic acid and patulin by an atypical strain of Penicillium roqueforti isolated from cheddar cheese is reported. Mycotoxin production was confirmed by thin-layer and gas-liquid chromatography and by ultraviolet, infrared, and mass spectral analyses. Culture extracts were toxic to Bacill...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.35.2.435-438.1978

    authors: Olivigni FJ,Bullerman LB

    更新日期:1978-02-01 00:00:00

  • Formation of 4-hydroxy-2,5-dimethyl-3[2H]-furanone by Zygosaccharomyces rouxii: identification of an intermediate.

    abstract::The formation of the important flavor compound 4-hydroxy-2,5-dimethyl-3[2H]-furanone (HDMF; Furaneol) from D-fructose-1,6-bisphosphate by the yeast Zygosaccharomyces rouxii was studied with regard to the identification of intermediates present in the culture medium. Addition of o-phenylenediamine, a trapping reagent f...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/aem.69.7.3911-3918.2003

    authors: Hauck T,Brühlmann F,Schwab W

    更新日期:2003-07-01 00:00:00

  • Isolation and identification of pathogenic Naegleria from Florida lakes.

    abstract::Five cases of primary amoebic meningoencephalitis associated with swimming in freshwater lakes have been recorded in Florida over the past 14 years. The present study demonstrated that pathogenic Naegleria, the causative agent, is relatively widespread. Twelve of 26 lakes sampled only once yielded the amoeba. Populati...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.34.6.661-667.1977

    authors: Wellings FM,Amuso PT,Chang SL,Lewis AL

    更新日期:1977-12-01 00:00:00

  • Survival of Campylobacter jejuni in water: effect of grazing by the freshwater crustacean Daphnia carinata (Cladocera).

    abstract::Environmental studies of the human-pathogenic bacterium Campylobacter jejuni have focused on linking distributions with potential sources. However, in aquatic ecosystems, the abundance of C. jejuni may also be regulated by predation. We examine the potential for grazing by the freshwater planktonic crustacean Daphnia ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.71.9.5085-5088.2005

    authors: Schallenberg M,Bremer PJ,Henkel S,Launhardt A,Burns CW

    更新日期:2005-09-01 00:00:00

  • Microbial transformation of polycyclic aromatic hydrocarbons in pristine and petroleum-contaminated sediments.

    abstract::To determine rates of microbial transformation of polycyclic aromatic hydrocarbons (PAH) in freshwater sediments, C-labeled PAH were incubated with samples from both pristine and petroleum-contaminated streams. Evolved CO(2) was trapped in KOH, unaltered PAH and polar metabolic intermediate fractions were quantitated ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.35.2.306-316.1978

    authors: Herbes SE,Schwall LR

    更新日期:1978-02-01 00:00:00

  • Modeling of pathogen survival during simulated gastric digestion.

    abstract::The objective of the present study was to develop a mathematical model of pathogenic bacterial inactivation kinetics in a gastric environment in order to further understand a part of the infectious dose-response mechanism. The major bacterial pathogens Listeria monocytogenes, Escherichia coli O157:H7, and Salmonella s...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.02139-10

    authors: Koseki S,Mizuno Y,Sotome I

    更新日期:2011-02-01 00:00:00

  • Molecular characterization of a toluene-degrading methanogenic consortium.

    abstract::A toluene-degrading methanogenic consortium enriched from creosote-contaminated aquifer material was maintained on toluene as the sole carbon and energy source for 10 years. The species in the consortium were characterized by using a molecular approach. Total genomic DNA was isolated, and 16S rRNA genes were amplified...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.65.12.5576-5585.1999

    authors: Ficker M,Krastel K,Orlicky S,Edwards E

    更新日期:1999-12-01 00:00:00

  • Marker rescue studies of the transfer of recombinant DNA to Streptococcus gordonii in vitro, in foods and gnotobiotic rats.

    abstract::A plasmid marker rescue system based on restoration of the nptII gene was established in Streptococcus gordonii to study the transfer of bacterial and transgenic plant DNA by transformation. In vitro studies revealed that the marker rescue efficiency depends on the type of donor DNA. Plasmid and chromosomal DNA of bac...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/aem.69.10.6121-6127.2003

    authors: Kharazmi M,Sczesny S,Blaut M,Hammes WP,Hertel C

    更新日期:2003-10-01 00:00:00

  • Some like it hot: evolution and ecology of novel endosymbionts in bat flies of cave-roosting bats (hippoboscoidea, nycterophiliinae).

    abstract::We investigated previously unknown associations between bacterial endosymbionts and bat flies of the subfamily Nycterophiliinae (Diptera, Streblidae). Molecular analyses revealed a novel clade of Gammaproteobacteria in Nycterophilia bat flies. This clade was not closely related to Arsenophonus-like microbes found in i...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.02455-12

    authors: Morse SF,Dick CW,Patterson BD,Dittmar K

    更新日期:2012-12-01 00:00:00

  • Alternative Sigma Factor RpoX Is a Part of the RpoE Regulon and Plays Distinct Roles in Stress Responses, Motility, Biofilm Formation, and Hemolytic Activities in the Marine Pathogen Vibrio alginolyticus.

    abstract::Vibrio alginolyticus is one of the most abundant microorganisms in marine environments and is also an opportunistic pathogen mediating high-mortality vibriosis in marine animals. Alternative sigma factors play essential roles in bacterial pathogens in the adaptation to environmental changes during infection and the ad...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.00234-19

    authors: Gu D,Zhang J,Hao Y,Xu R,Zhang Y,Ma Y,Wang Q

    更新日期:2019-07-01 00:00:00

  • Intraclade Variability in Toxin Production and Cytotoxicity of Bacillus cereus Group Type Strains and Dairy-Associated Isolates.

    abstract::While some species in the Bacillus cereus group are well-characterized human pathogens (e.g., B. anthracis and B. cereus sensu stricto), the pathogenicity of other species (e.g., B. pseudomycoides) either has not been characterized or is presently not well understood. To provide an updated characterization of the path...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.02479-17

    authors: Miller RA,Jian J,Beno SM,Wiedmann M,Kovac J

    更新日期:2018-03-01 00:00:00

  • Metabolism of phosphonoacetate as the sole carbon and phosphorus source by an environmental bacterial isolate.

    abstract::A gram-negative bacterium isolated from activated sludge was able to utilize up to 25 mM phosphonoacetate as the sole carbon and phosphorus source, with simultaneous excretion of virtually equimolar levels of phosphate. 2-Aminoethylphosphonate was similarly utilized with equivalent growth rates and cellular yields, wh...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.58.4.1364-1366.1992

    authors: McMullan G,Harrington F,Quinn JP

    更新日期:1992-04-01 00:00:00

  • Genetic diversity in marine algal virus communities as revealed by sequence analysis of DNA polymerase genes.

    abstract::Algal-virus-specific PCR primers were used to amplify DNA polymerase gene (pol) fragments (683 to 689 bp) from the virus-sized fraction (0.02 to 0.2 microns) concentrated from inshore and offshore water samples collected from the Gulf of Mexico. Algal-virus-like DNA pol genes were detected in five samples collected fr...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.62.8.2869-2874.1996

    authors: Chen F,Suttle CA,Short SM

    更新日期:1996-08-01 00:00:00

  • New Selective Growth Inhibitors of Sphaerotilus natans, Anslimins A and B.

    abstract::Selective growth inhibitors of Sphaerotilus natans were detected in the culture broths of several strains of Streptomyces. Anslimins A and B were isolated from the culture broth of Streptomyces oganonensis by adsorption on resinous adsorbent followed by elution with 50% aqueous acetone. The A and B components were the...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.39.6.1123-1128.1980

    authors: Takiguchi Y,Yoshikawa H,Terao M

    更新日期:1980-06-01 00:00:00

  • Metabolic and Genomic Traits of Phytobeneficial Phenazine-Producing Pseudomonas spp. Are Linked to Rhizosphere Colonization in Arabidopsis thaliana and Solanum tuberosum.

    abstract::Bacterial rhizosphere colonization is critical for phytobeneficial rhizobacteria such as phenazine-producing Pseudomonas spp. To better understand this colonization process, potential metabolic and genomic determinants required for rhizosphere colonization were identified using a collection of 60 phenazine-producing P...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.02443-19

    authors: Zboralski A,Biessy A,Savoie MC,Novinscak A,Filion M

    更新日期:2020-02-03 00:00:00

  • Mycoplasma agalactiae Secretion of β-(1→6)-Glucan, a Rare Polysaccharide in Prokaryotes, Is Governed by High-Frequency Phase Variation.

    abstract:UNLABELLED:Mycoplasmas are minimal, wall-less bacteria but have retained the ability to secrete complex carbohydrate polymers that constitute a glycocalyx. In members of the Mycoplasma mycoides cluster, which are important ruminant pathogens, the glycocalyx includes both cell-attached and cell-free polysaccharides. Thi...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.00274-16

    authors: Gaurivaud P,Baranowski E,Pau-Roblot C,Sagné E,Citti C,Tardy F

    更新日期:2016-05-16 00:00:00

  • Presence of enterohemorrhagic Escherichia coli ST678/O104:H4 in France prior to 2011.

    abstract::Two isolates of enterohemorrhagic Escherichia coli (EHEC) O104:H4 were isolated in France in 2004 and 2009. Both were characterized and compared to the strain which caused the German outbreak in 2011 and to other O104:H4 strains. This suggests that different O104:H4 EHEC strains were present several years prior to the...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.06524-11

    authors: Monecke S,Mariani-Kurkdjian P,Bingen E,Weill FX,Balière C,Slickers P,Ehricht R

    更新日期:2011-12-01 00:00:00

  • Expression of the Escherichia coli pntA and pntB genes, encoding nicotinamide nucleotide transhydrogenase, in Saccharomyces cerevisiae and its effect on product formation during anaerobic glucose fermentation.

    abstract::We studied the physiological effect of the interconversion between the NAD(H) and NADP(H) coenzyme systems in recombinant Saccharomyces cerevisiae expressing the membrane-bound transhydrogenase from Escherichia coli. Our objective was to determine if the membrane-bound transhydrogenase could work in reoxidation of NAD...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.65.6.2333-2340.1999

    authors: Anderlund M,Nissen TL,Nielsen J,Villadsen J,Rydström J,Hahn-Hägerdal B,Kielland-Brandt MC

    更新日期:1999-06-01 00:00:00

  • Analysis of fecal populations of bifidobacteria and lactobacilli and investigation of the immunological responses of their human hosts to the predominant strains.

    abstract::The bifidobacterial and lactobacillus populations of fecal samples collected from 10 human subjects were studied. The numbers of bifidobacteria were similar in the fecal samples of all of the subjects, but lactobacillus numbers varied, even between samples collected from the same individual. Analysis of the compositio...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.63.9.3394-3398.1997

    authors: Kimura K,McCartney AL,McConnell MA,Tannock GW

    更新日期:1997-09-01 00:00:00

  • Cross-institute evaluations of inhibitor-resistant PCR reagents for direct testing of aerosol and blood samples containing biological warfare agent DNA.

    abstract::Rapid pathogen detection is crucial for the timely introduction of therapeutics. Two groups (one in the United Kingdom and one in the United States) independently evaluated inhibitor-resistant PCR reagents for the direct testing of substrates. In the United Kingdom, a multiplexed Bacillus anthracis (target) and Bacill...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.03478-13

    authors: Minogue TD,Rachwal PA,Trombley Hall A,Koehler JW,Weller SA

    更新日期:2014-02-01 00:00:00

  • Bacillus thuringiensis Cry1Ab Domain III β-22 Mutants with Enhanced Toxicity to Spodoptera frugiperda (J. E. Smith).

    abstract::The fall armyworm, Spodoptera frugiperda, is an invasive maize pest that has spread from the Americas into Africa and Asia and causes severe crop damage worldwide. Most populations of S. frugiperda show low susceptibility to Bacillus thuringiensis (Bt) Cry1Ab or Cry1Ac toxins, which have been proved to be effective ag...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.01580-20

    authors: Gómez I,Ocelotl J,Sánchez J,Aguilar-Medel S,Peña-Chora G,Lina-Garcia L,Bravo A,Soberón M

    更新日期:2020-10-28 00:00:00

  • Catalase and superoxide dismutase of root-colonizing saprophytic fluorescent pseudomonads.

    abstract::Root-colonizing, saprophytic fluorescent pseudomonads of the Pseudomonas putida-P. fluorescens group express similar levels of catalase and superoxide dismutase activities during growth on a sucrose- and amino acid-rich medium. Increased specific activities of catalase but not superoxide dismutase were observed during...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.56.11.3576-3582.1990

    authors: Katsuwon J,Anderson AJ

    更新日期:1990-11-01 00:00:00

  • Reduction of Escherichia coli O157:H7 populations in cattle by addition of colicin E7-producing E. coli to feed.

    abstract::A cattle trial using artificially inoculated calves was conducted to determine the effect of the addition of colicinogenic Escherichia coli strains capable of producing colicin E7 (a 61-kDa DNase) to feed on the fecal shedding of serotype O157:H7. The experiment was divided into three periods. In period 1, which laste...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.70.10.6053-6060.2004

    authors: Schamberger GP,Phillips RL,Jacobs JL,Diez-Gonzalez F

    更新日期:2004-10-01 00:00:00

  • Evaluation of broiler litter with reference to the microbial composition as assessed by using 16S rRNA and functional gene markers.

    abstract::Very little is known about the microbial composition of animal bedding wastes, including poultry litter, and what is known has been deduced from standard culture methods, by which some fastidious organisms that exist in the environment may not be detected. We evaluated the bacterial composition of poultry litter by us...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/aem.69.2.901-908.2003

    authors: Lu J,Sanchez S,Hofacre C,Maurer JJ,Harmon BG,Lee MD

    更新日期:2003-02-01 00:00:00