Expression of the Escherichia coli pntA and pntB genes, encoding nicotinamide nucleotide transhydrogenase, in Saccharomyces cerevisiae and its effect on product formation during anaerobic glucose fermentation.

Abstract:

:We studied the physiological effect of the interconversion between the NAD(H) and NADP(H) coenzyme systems in recombinant Saccharomyces cerevisiae expressing the membrane-bound transhydrogenase from Escherichia coli. Our objective was to determine if the membrane-bound transhydrogenase could work in reoxidation of NADH to NAD+ in S. cerevisiae and thereby reduce glycerol formation during anaerobic fermentation. Membranes isolated from the recombinant strains exhibited reduction of 3-acetylpyridine-NAD+ by NADPH and by NADH in the presence of NADP+, which demonstrated that an active enzyme was present. Unlike the situation in E. coli, however, most of the transhydrogenase activity was not present in the yeast plasma membrane; rather, the enzyme appeared to remain localized in the membrane of the endoplasmic reticulum. During anaerobic glucose fermentation we observed an increase in the formation of 2-oxoglutarate, glycerol, and acetic acid in a strain expressing a high level of transhydrogenase, which indicated that increased NADPH consumption and NADH production occurred. The intracellular concentrations of NADH, NAD+, NADPH, and NADP+ were measured in cells expressing transhydrogenase. The reduction of the NADPH pool indicated that the transhydrogenase transferred reducing equivalents from NADPH to NAD+.

journal_name

Appl Environ Microbiol

authors

Anderlund M,Nissen TL,Nielsen J,Villadsen J,Rydström J,Hahn-Hägerdal B,Kielland-Brandt MC

doi

10.1128/AEM.65.6.2333-2340.1999

keywords:

subject

Has Abstract

pub_date

1999-06-01 00:00:00

pages

2333-40

issue

6

eissn

0099-2240

issn

1098-5336

journal_volume

65

pub_type

杂志文章
  • Transformation of 2,4,6-Trinitrotoluene by Pseudomonas pseudoalcaligenes JS52.

    abstract::Pseudomonas pseudoalcaligenes JS52 grows on nitrobenzene via partial reduction of the nitro group and enzymatic rearrangement of the resultant hydroxylamine. Cells and cell extracts of nitrobenzene-grown JS52 catalyzed the transient formation of 4-hydroxylamino-2,6-dinitrotoluene (4HADNT), 4-amino-2,6-dinitrotoluene (...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.63.5.2007-2015.1997

    authors: Fiorella PD,Spain JC

    更新日期:1997-05-01 00:00:00

  • Inexpensive laboratory-constructed nephelometer.

    abstract::An inexpensive, simple-to-construct nephelometer which was used to monitor the lysis of spheroplasts is described. The nephelometer is a flow-through device with a linear response to cell concentration from the lower detection limit to 8 x 10(8) cells per ml. ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.44.6.1476-1478.1982

    authors: Smith TL,Witter LD

    更新日期:1982-12-01 00:00:00

  • Structural basis of the divergent oxygenation reactions catalyzed by the rieske nonheme iron oxygenase carbazole 1,9a-dioxygenase.

    abstract::Carbazole 1,9a-dioxygenase (CARDO), a Rieske nonheme iron oxygenase (RO), is a three-component system composed of a terminal oxygenase (Oxy), ferredoxin, and a ferredoxin reductase. Oxy has angular dioxygenation activity against carbazole. Previously, site-directed mutagenesis of the Oxy-encoding gene from Janthinobac...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.04000-13

    authors: Inoue K,Usami Y,Ashikawa Y,Noguchi H,Umeda T,Yamagami-Ashikawa A,Horisaki T,Uchimura H,Terada T,Nakamura S,Shimizu K,Habe H,Yamane H,Fujimoto Z,Nojiri H

    更新日期:2014-05-01 00:00:00

  • Shigella sonnei Does Not Use Amoebae as Protective Hosts.

    abstract::Shigella flexneri and Shigella sonnei bacteria cause the majority of all shigellosis cases worldwide. However, their distributions differ, with S. sonnei predominating in middle- and high-income countries and S. flexneri predominating in low-income countries. One proposed explanation for the continued range expansion ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.02679-17

    authors: Watson J,Jenkins C,Clements A

    更新日期:2018-04-16 00:00:00

  • Divergence of Genomic Sequences between Lactococcus lactis subsp. lactis and Lactococcus lactis subsp. cremoris.

    abstract::Relatedness between Lactococcus lactis subsp. cremoris and L. lactis subsp. lactis was assessed by Southern hybridization analysis, with cloned chromosomal genes as probes. The results indicate that strains of the two subspecies form two distinct groups and that the DNA sequence divergence between L. lactis subsp. lac...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.58.12.4045-4047.1992

    authors: Godon JJ,Delorme C,Ehrlich SD,Renault P

    更新日期:1992-12-01 00:00:00

  • Integrative food-grade expression system based on the lactose regulon of Lactobacillus casei.

    abstract::The lactose operon from Lactobacillus casei is regulated by very tight glucose repression and substrate induction mechanisms, which made it a tempting candidate system for the expression of foreign genes or metabolic engineering. An integrative vector was constructed, allowing stable gene insertion in the chromosomal ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/aem.66.11.4822-4828.2000

    authors: Gosalbes MJ,Esteban CD,Galán JL,Pérez-Martínez G

    更新日期:2000-11-01 00:00:00

  • Catabolite repression control of pyocyanin biosynthesis at an intersection of primary and secondary metabolism in Pseudomonas aeruginosa.

    abstract::In Pseudomonas aeruginosa, the catabolite repression control (Crc) protein repressed the formation of the blue pigment pyocyanin in response to a preferred carbon source (succinate) by interacting with phzM mRNA, which encodes a key enzyme in pyocyanin biosynthesis. Crc bound to an extended imperfect recognition seque...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.00026-12

    authors: Huang J,Sonnleitner E,Ren B,Xu Y,Haas D

    更新日期:2012-07-01 00:00:00

  • A Novel Glaesserella sp. Isolated from Pigs with Severe Respiratory Infections Has a Mosaic Genome with Virulence Factors Putatively Acquired by Horizontal Transfer.

    abstract::An unknown member of the family Pasteurellaceae was repeatedly isolated from 20- to 24-week-old pigs with severe pulmonary lesions reared on the same farm in Victoria, Australia. The etiological diagnosis of the disease was inconclusive. The complete genome sequence analysis of one strain, 15-184, revealed some phylog...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.00092-18

    authors: Watt AE,Browning GF,Legione AR,Bushell RN,Stent A,Cutler RS,Young ND,Marenda MS

    更新日期:2018-05-17 00:00:00

  • Se(VI) reduction and the precipitation of Se(0) by the facultative bacterium Enterobacter cloacae SLD1a-1 are regulated by FNR.

    abstract::The fate of selenium in the environment is controlled, in part, by microbial selenium oxyanion reduction and Se(0) precipitation. In this study, we identified a genetic regulator that controls selenate reductase activity in the Se-reducing bacterium Enterobacter cloacae SLD1a-1. Heterologous expression of the global a...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.02542-06

    authors: Yee N,Ma J,Dalia A,Boonfueng T,Kobayashi DY

    更新日期:2007-03-01 00:00:00

  • Expanded genetic map of Gibberella moniliformis (Fusarium verticillioides).

    abstract::Gibberella moniliformis (Fusarium verticillioides) is primarily a pathogen of maize, but it can also cause disease in other crop species. This pathogenicity, as well as the contamination of food- and feedstuffs with the fumonisin mycotoxins, results in economically significant losses to both farmers and food processor...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/aem.68.4.1972-1979.2002

    authors: Jurgenson JE,Zeller KA,Leslie JF

    更新日期:2002-04-01 00:00:00

  • Studies on an acetate-fermenting strain of Methanosarcina.

    abstract::An acetate-fermenting strain of Methanosarcina was isolated from an acetate enrichment culture inoculated with anaerobic sludge from a waste treatment digestor. In pure culture, this organism fermented acetate in the absence of added hydrogen at rates comparable in magnitude to those found in digestor systems. This ra...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.35.6.1174-1184.1978

    authors: Mah RA,Smith MR,Baresi L

    更新日期:1978-06-01 00:00:00

  • Molecular and genetic characterization of propionicin F, a bacteriocin from Propionibacterium freudenreichii.

    abstract::This work describes the purification and characterization of propionicin F, the first bacteriocin isolated from Propionibacterium freudenreichii. The bacteriocin has a bactericidal activity and is only active against strains of P. freudenreichii. Propionicin F appears to be formed through a processing pathway new to b...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.70.12.7303-7310.2004

    authors: Brede DA,Faye T,Johnsborg O,Odegård I,Nes IF,Holo H

    更新日期:2004-12-01 00:00:00

  • Metabolic behavior of Lactococcus lactis MG1363 in microaerobic continuous cultivation at a low dilution rate.

    abstract::Minute amounts of oxygen were supplied to a continuous cultivation of Lactococcus lactis subsp. cremoris MG1363 grown on a defined glucose-limited medium at a dilution rate of 0.1 h(-1). More than 80% of the carbon supplied with glucose ended up in fermentation products other than lactate. Addition of even minute amou...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.67.6.2677-2682.2001

    authors: Jensen NB,Melchiorsen CR,Jokumsen KV,Villadsen J

    更新日期:2001-06-01 00:00:00

  • Characterization of a latent virus-like infection of symbiotic zooxanthellae.

    abstract::A latent virus-like agent, which we designated zooxanthella filamentous virus 1 (ZFV1), was isolated from Symbiodinium sp. strain CCMP 2465 and characterized. Transmission electron microscopy and analytical flow cytometry revealed the presence of a new group of distinctive filamentous virus-like particles after exposu...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.02449-06

    authors: Lohr J,Munn CB,Wilson WH

    更新日期:2007-05-01 00:00:00

  • Characterization of a thermostable D-stereospecific alanine amidase from Brevibacillus borstelensis BCS-1.

    abstract::A gene encoding a new thermostable D-stereospecific alanine amidase from the thermophile Brevibacillus borstelensis BCS-1 was cloned and sequenced. The molecular mass of the purified enzyme was estimated to be 199 kDa after gel filtration chromatography and about 30 kDa on sodium dodecyl sulfate-polyacrylamide gel ele...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/aem.69.2.980-986.2003

    authors: Baek DH,Kwon SJ,Hong SP,Kwak MS,Lee MH,Song JJ,Lee SG,Yoon KH,Sung MH

    更新日期:2003-02-01 00:00:00

  • Vector Competence of Geographical Populations of Ornithodoros turicata for the Tick-Borne Relapsing Fever Spirochete Borrelia turicatae.

    abstract::Vector competence refers to the ability of an arthropod to acquire, maintain, and successfully transmit a microbial pathogen. Tick-borne relapsing fever (TBRF) spirochetes are globally distributed pathogens, and most species are transmitted by argasid ticks of the genus Ornithodoros. A defining characteristic in vecto...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.01505-18

    authors: Krishnavajhala A,Armstrong BA,Lopez JE

    更新日期:2018-10-17 00:00:00

  • Bacterial fermentation of cheese whey for production of a ruminant feed supplement rich in curde protein.

    abstract::A simple and efficient process for the production of a ruminant feed supplement, rich in crude protein (defined as total N X 6.25), by bacterial fermentation of cheese whey has been developed. The lactose in unpasteurized whey is fermented to lactate acid by Lactobacillus bulgaricus at a temperature of 43 degrees C an...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.32.6.769-776.1976

    authors: Reddy CA,Henderson HE,Erdman MD

    更新日期:1976-12-01 00:00:00

  • Determination of the efficacy of two building decontamination strategies by surface sampling with culture and quantitative PCR analysis.

    abstract::The efficacy of currently available decontamination strategies for the treatment of indoor furnishings contaminated with bioterrorism agents is poorly understood. Efficacy testing of decontamination products in a controlled environment is needed to ensure that effective methods are used to decontaminate domestic and w...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.70.8.4740-4747.2004

    authors: Buttner MP,Cruz P,Stetzenbach LD,Klima-Comba AK,Stevens VL,Cronin TD

    更新日期:2004-08-01 00:00:00

  • Detection of airborne Stachybotrys chartarum macrocyclic trichothecene mycotoxins in the indoor environment.

    abstract::The existence of airborne mycotoxins in mold-contaminated buildings has long been hypothesized to be a potential occupant health risk. However, little work has been done to demonstrate the presence of these compounds in such environments. The presence of airborne macrocyclic trichothecene mycotoxins in indoor environm...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.71.11.7376-7388.2005

    authors: Brasel TL,Martin JM,Carriker CG,Wilson SC,Straus DC

    更新日期:2005-11-01 00:00:00

  • Response of Archaeal communities in beach sediments to spilled oil and bioremediation.

    abstract::While the contribution of Bacteria to bioremediation of oil-contaminated shorelines is well established, the response of Archaea to spilled oil and bioremediation treatments is unknown. The relationship between archaeal community structure and oil spill bioremediation was examined in laboratory microcosms and in a bio...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/aem.70.5.2614-2620.2004

    authors: Röling WF,de Brito Couto IR,Swannell RP,Head IM

    更新日期:2004-05-01 00:00:00

  • Formate-dependent growth and homoacetogenic fermentation by a bacterium from human feces: description of Bryantella formatexigens gen. nov., sp. nov.

    abstract::Formate stimulates growth of a new bacterium from human feces. With high formate, it ferments glucose to acetate via the Wood-Ljungdahl pathway. The original isolate fermented vegetable cellulose and carboxymethylcellulose, but it lost this ability after storage at -76 degrees C. 16S rRNA gene sequencing identifies it...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/aem.69.10.6321-6326.2003

    authors: Wolin MJ,Miller TL,Collins MD,Lawson PA

    更新日期:2003-10-01 00:00:00

  • Effects of pesticides on cyanobacterium Plectonema boryanum and cyanophage LPP-1.

    abstract::Cyanobacterium Plectonema boryanum IU 594 and cyanophage LPP-1 were used as indicator organisms in a bioassay of 16 pesticides. Experiments such as spot tests, disk assays, growth curves, and one-step growth experiments were used to examine the effects of pesticides on the host and virus. Also, experiments were done i...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.47.5.910-914.1984

    authors: Mallison SM 3rd,Cannon RE

    更新日期:1984-05-01 00:00:00

  • Chloromethane utilization gene cluster from Hyphomicrobium chloromethanicum strain CM2(T) and development of functional gene probes to detect halomethane-degrading bacteria.

    abstract::Hyphomicrobium chloromethanicum CM2(T), an aerobic methylotrophic member of the alpha subclass of the class proteobacteria, can grow with chloromethane as the sole carbon and energy source. H. chloromethanicum possesses an inducible enzyme system for utilization of chloromethane, in which two polypeptides (67-kDa CmuA...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.67.1.307-316.2001

    authors: McAnulla C,Woodall CA,McDonald IR,Studer A,Vuilleumier S,Leisinger T,Murrell JC

    更新日期:2001-01-01 00:00:00

  • Microbial community shifts reflect losses of native soil carbon with pyrogenic and fresh organic matter additions and are greatest in low-carbon soils.

    abstract::Soil organic carbon (SOC) plays an important role in regulating global climate change, carbon and nutrient cycling in soils, and soil moisture. Organic matter (OM) additions to soils can affect the rate at which SOC is mineralized by microbes, with potentially important effects on SOC stocks. Understanding how pyrogen...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.02555-20

    authors: Whitman T,DeCiucies S,Hanley K,Enders A,Woolet J,Lehmann J

    更新日期:2021-01-29 00:00:00

  • Characterization of the microbial community colonizing the anal and vulvar pores of helminths from the hindgut of zebras.

    abstract::Scanning and transmission electron microscopy were used to examine the adherence and in situ morphology of the microbial community colonizing the anal and vulvar pores of the subfamily Cyathostominae (Nematoda: Strongylidae) from the colon of Burchell's zebra (Equus burchelli antiquorum). Two different morphological t...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.55.5.1178-1186.1989

    authors: Mackie RI,Krecek RC,Els HJ,van Niekerk JP,Kirschner LM,Baecker AA

    更新日期:1989-05-01 00:00:00

  • Application of a pH-sensitive fluoroprobe (C-SNARF-4) for pH microenvironment analysis in Pseudomonas aeruginosa biofilms.

    abstract::An important feature of microbial biofilms is the development of four-dimensional physical and chemical gradients in space and time. There is need for novel approaches to probe these so-called microenvironments to determine their effect on biofilm-specific processes. In this study, we describe the use of seminaphthorh...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.71.5.2501-2510.2005

    authors: Hunter RC,Beveridge TJ

    更新日期:2005-05-01 00:00:00

  • National field evaluation of a defined substrate method for the simultaneous enumeration of total coliforms and Escherichia coli from drinking water: comparison with the standard multiple tube fermentation method.

    abstract::A defined substrate method was developed to simultaneously enumerate total coliforms and Escherichia coli from drinking waters without the need for confirmatory or completed tests. It is a new method based on technology that uses a hydrolyzable substrate as a specific indicator-nutrient for the target microbes. No equ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.54.6.1595-1601.1988

    authors: Edberg SC,Allen MJ,Smith DB

    更新日期:1988-06-01 00:00:00

  • Production of Nivalenol and Fusarenone-X by Fusarium tricinctum Fn-2B on a Rice Substrate.

    abstract::Fusarium tricinctum Fn-2B was grown on a rice substrate at room temperature (22 to 26 degrees C) for 2 weeks followed by growth at a low temperature (10 to 12 degrees C) for an additional 2 weeks. A total of 1.5 g of nivalenol and 80 mg of fusarenone-X were obtained from 2 kg of rice culture. The methods of production...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.48.4.857-858.1984

    authors: Lee YW,Mirocha CJ

    更新日期:1984-10-01 00:00:00

  • Use of a species-specific DNA hybridization probe for enumerating Bacteroides vulgatus in human feces.

    abstract::pBV-1, a recombinant plasmid that contains a chromosomal DNA fragment from Bacteroides vulgatus, hybridized to DNA from B. vulgatus but not to DNA from other colonic Bacteroides species. This plasmid was used as a DNA probe to detect and enumerate B. vulgatus in pure culture, in mixed cultures, and in a bacterial frac...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.50.4.958-964.1985

    authors: Kuritza AP,Salyers AA

    更新日期:1985-10-01 00:00:00

  • The Legionella pneumophila collagen-like protein mediates sedimentation, autoaggregation, and pathogen-phagocyte interactions.

    abstract::Although only partially understood, multicellular behavior is relatively common in bacterial pathogens. Bacterial aggregates can resist various host defenses and colonize their environment more efficiently than planktonic cells. For the waterborne pathogen Legionella pneumophila, little is known about the roles of aut...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.03254-13

    authors: Abdel-Nour M,Duncan C,Prashar A,Rao C,Ginevra C,Jarraud S,Low DE,Ensminger AW,Terebiznik MR,Guyard C

    更新日期:2014-02-01 00:00:00