Characterization of the microbial community colonizing the anal and vulvar pores of helminths from the hindgut of zebras.

abstract：:A multiplex fluorogenic PCR assay for simultaneous detection of pathogenic Salmonella strains and Escherichia coli O157:H7 was developed and evaluated for use in detecting very low levels of these pathogens in meat and feces. Two sets of primers were used to amplify a junctional segment of virulence genes sipB and sip...

journal_title：Applied and environmental microbiology

authors： Sharma VK,Carlson SA

更新日期：2000-12-01 00:00:00

abstract：:Pseudomonas syringae pv. actinidiae is a reemerging pathogen which causes bacterial canker of kiwifruit (Actinidia sp.). Since 2008, a global outbreak of P. syringae pv. actinidiae has occurred, and in 2010 this pathogen was detected in New Zealand. The economic impact and the development of resistance in P. syringae ...

journal_title：Applied and environmental microbiology

authors： Frampton RA,Taylor C,Holguín Moreno AV,Visnovsky SB,Petty NK,Pitman AR,Fineran PC

更新日期：2014-04-01 00:00:00

abstract：:Psychrophilic, gas vacuolate, heterotrophic bacteria indigenous to sea ice communities in Antarctica have been isolated. Phylogenetic analysis of representative members of these bacteria shows that they belong to the alpha, beta, and gamma Proteobacteria and the Flavobacteria-Cytophaga group. This is the first report ...

journal_title：Applied and environmental microbiology

authors： Gosink JJ,Staley JT

更新日期：1995-09-01 00:00:00

abstract：:The relationship between physiochemical surface parameters and adhesion of bacterial cells to negatively charged polystyrene was studied. Cell surface hydrophobicity and electrokinetic potential were determined by contact angle measurement and electrophoresis, respectively. Both parameters influence cell adhesion. The...

journal_title：Applied and environmental microbiology

authors： van Loosdrecht MC,Lyklema J,Norde W,Schraa G,Zehnder AJ

更新日期：1987-08-01 00:00:00

abstract：:Lactose is the only soluble and economically feasible carbon source for the production of cellulases or heterologous proteins regulated by cellulase expression signals by Hypocrea jecorina (Trichoderma reesei). We investigated the role of the major beta-galactosidase of H. jecorina in lactose metabolism and cellulase ...

journal_title：Applied and environmental microbiology

authors： Seiboth B,Hartl L,Salovuori N,Lanthaler K,Robson GD,Vehmaanperä J,Penttilä ME,Kubicek CP

更新日期：2005-02-01 00:00:00

abstract：:Significant knowledge gaps exist in our understanding of Campylobacter jejuni contamination of the poultry production continuum. Microbiological surveillance and genotypic characterization were undertaken on C. jejuni isolates longitudinally recovered from three poultry farms (weekly samples), the abattoir at which bi...

journal_title：Applied and environmental microbiology

authors： Inglis GD,Ramezani N,Taboada EN,Boras VF,Uwiera RRE

更新日期：2021-01-15 00:00:00

abstract：:The only major structural protein (35 kDa) of the lactococcal small isometric-headed bacteriophage ul36, a member of the P335 species, was isolated from a preparative sodium dodecyl sulfate (SDS)-polyacrylamide gel electrophoresis. Monoclonal antibodies (MAbs) were raised against the denatured 35-kDa protein. Six MAbs...

journal_title：Applied and environmental microbiology

authors： Moineau S,Bernier D,Jobin M,Hébert J,Klaenhammer TR,Pandian S

更新日期：1993-07-01 00:00:00

abstract：:A group-specific primer, F243 (positions 226 to 243, Escherichia coli numbering), was developed by comparison of sequences of genes encoding 16S rRNA (16S rDNA) for the detection of actinomycetes in the environment with PCR and temperature or denaturing gradient gel electrophoresis (TGGE or DGGE, respectively). The sp...

journal_title：Applied and environmental microbiology

authors： Heuer H,Krsek M,Baker P,Smalla K,Wellington EM

更新日期：1997-08-01 00:00:00

abstract：:Auxotrophic microorganisms are often used for genetic engineering, because their biosynthetic deficiency can be complemented by the transforming DNA and allows selection for transformants that have become prototrophic. However, when complementation is obtained by ectopic expression this may lead to unpredictable side ...

journal_title：Applied and environmental microbiology

authors： Nielsen ML,de Jongh WA,Meijer SL,Nielsen J,Mortensen UH

更新日期：2007-11-01 00:00:00

abstract：:We found that a robust energy taxis response mediated by the Aer receptor can sometimes mask chemotaxis mediated by other methyl-accepting chemotaxis proteins (MCPs) in Pseudomonas aeruginosa. We identified PA2652 as a chemoreceptor for malate by screening aer mcp double mutants by using swarm plate assays. ...

journal_title：Applied and environmental microbiology

authors： Alvarez-Ortega C,Harwood CS

更新日期：2007-12-01 00:00:00

abstract：:A variety of lactic acid bacteria, isolated from plant surfaces and plant-associated products, were found to be antagonistic to test strains of the phytopathogens Xanthomonas campestris, Erwinia carotovora, and Pseudomonas syringae. Effective "in vitro" inhibition was found both on agar plates and in broth cultures. I...

journal_title：Applied and environmental microbiology

authors： Visser R,Holzapfel WH,Bezuidenhout JJ,Kotzé JM

更新日期：1986-09-01 00:00:00

abstract：:Biofilms are composed of bacterial cells encased in a self-synthesized, extracellular polymeric matrix. Poly-beta(1,6)-N-acetyl-d-glucosamine (PNAG) is a major biofilm matrix component in phylogenetically diverse bacteria. In this study we investigated the physical and chemical properties of the PNAG matrix in biofilm...

journal_title：Applied and environmental microbiology

authors： Ganeshnarayan K,Shah SM,Libera MR,Santostefano A,Kaplan JB

更新日期：2009-03-01 00:00:00

abstract：:Pseudomonas oleovorans grows on C(6) to C(12)n-alkanes and 1-alkenes. These substrates are oxidized to the corresponding fatty acids, which are oxidized further via the beta-oxidation pathway, yielding shorter fatty acids which have lost one or more C(2) units. P. oleovorans normally utilizes beta-oxidation pathway in...

journal_title：Applied and environmental microbiology

authors： Lageveen RG,Huisman GW,Preusting H,Ketelaar P,Eggink G,Witholt B

更新日期：1988-12-01 00:00:00

abstract：:Cyanuric acid is an industrial chemical produced during the biodegradation of s-triazine pesticides. The biodegradation of cyanuric acid has been elucidated using a single model system, Pseudomonas sp. strain ADP, in which cyanuric acid hydrolase (AtzD) opens the s-triazine ring and AtzEG deaminates the ring-opened pr...

journal_title：Applied and environmental microbiology

authors： Aukema KG,Tassoulas LJ,Robinson SL,Konopatski JF,Bygd MD,Wackett LP

更新日期：2020-01-07 00:00:00

abstract：:Water delivered by dental unit water systems (DUWS) in general dental practices can harbor high numbers of bacteria, including opportunistic pathogens. Biofilms on tubing within DUWS provide a reservoir for microorganisms and should be controlled. This study compared disinfection products for their ability to meet the...

journal_title：Applied and environmental microbiology

authors： Schel AJ,Marsh PD,Bradshaw DJ,Finney M,Fulford MR,Frandsen E,Østergaard E,ten Cate JM,Moorer WR,Mavridou A,Kamma JJ,Mandilara G,Stösser L,Kneist S,Araujo R,Contreras N,Goroncy-Bermes P,O'Mullane D,Burke F,O'Reilly P

更新日期：2006-02-01 00:00:00

abstract：:Nonselected and natural populations of Escherichia coli from 12 animal sources and humans were examined for the presence and types of 14 tetracycline resistance determinants. Of 1,263 unique E. coli isolates from humans, pigs, chickens, turkeys, sheep, cows, goats, cats, dogs, horses, geese, ducks, and deer, 31% were ...

journal_title：Applied and environmental microbiology

authors： Bryan A,Shapir N,Sadowsky MJ

更新日期：2004-04-01 00:00:00

abstract：:Butyrate is an important intermediate in the anaerobic degradation of organic matter. In sulfate-depleted environments butyrate is oxidized to acetate and hydrogen by obligate proton reducers, in syntrophic association with hydrogen-consuming methanogens. This paper describes two enrichments of endospore-forming bacte...

journal_title：Applied and environmental microbiology

authors： Tomei FA,Maki JS,Mitchell R

更新日期：1985-11-01 00:00:00

abstract：:Cellobiose labeled either at the reducing end (4-O-beta-d-glucopyranosyl-d-[U-C]glucopyranose) or at the nonreducing end (4-O-beta-d-[U-C]glucopyranosyl-d -glucopyranose) was synthesized with Clostridium thermocellum cellobiose phosphorylase at greater than 98% purity. The radioactive cellobioses were identical to aut...

journal_title：Applied and environmental microbiology

authors： Ng TK,Zeikus JG

更新日期：1986-10-01 00:00:00

abstract：:Isolates of Paecilomyces, Verticillium, Beauveria, and Penicillium species were tested for ability to metabolize a variety of n-alkylbenzenes. Minimum side chain lengths were required for metabolism of these substrates. These were C(4) for the Paecilomyces sp., C(8) for the Verticillium sp., and C(9) for the other two...

journal_title：Applied and environmental microbiology

authors： Fedorak PM,Westlake DW

更新日期：1986-02-01 00:00:00

abstract：:Currently, it is estimated that only 0.001% to 15% of bacteria in any given system can be cultured by use of commonly used techniques and media, yet culturing is critically important for investigations of bacterial function. Despite this situation, few studies have attempted to link culture-dependent and culture-indep...

journal_title：Applied and environmental microbiology

authors： Walke JB,Becker MH,Hughey MC,Swartwout MC,Jensen RV,Belden LK

更新日期：2015-10-01 00:00:00

abstract：:We examined growth of mixed microbial cultures (13 fungal species and one actinomycete species) and production of volatile compounds (VOCs) in typical building materials in outside walls, separating walls, and bathroom floors at various relative humidities (RHs) of air. Air samples from incubation chambers were adsorb...

journal_title：Applied and environmental microbiology

authors： Korpi A,Pasanen AL,Pasanen P

更新日期：1998-08-01 00:00:00

abstract：:Culture-independent molecular phylogenetic methods were used to explore the breadth of diversity and environmental distribution of members of the division-level "candidate" phylogenetic group WS6, recently discovered in a contaminated aquifer and with no cultivated representatives. A broad diversity of WS6-affiliated ...

journal_title：Applied and environmental microbiology

authors： Dojka MA,Harris JK,Pace NR

更新日期：2000-04-01 00:00:00

abstract：:Chemolithoautotrophic bacteria were enriched from mine water at incubation temperatures ranging from 4 to 46 degrees C, using elemental sulfur as a substrate in acid mineral salts media. Thiobacillus-type bacteria were successfully enriched for at all test temperatures except 46 degrees C. Changes in pH (-dpH/dt) were...

journal_title：Applied and environmental microbiology

authors： Ahonen L,Tuovinen OH

更新日期：1990-02-01 00:00:00

abstract：:The microbial community composition of Wadden Sea sediments of the German North Sea coast was investigated by in situ hybridization with group-specific fluorescently labeled, rRNA-targeted oligonucleotides. A large fraction (up to 73%) of the DAPI (4',6-diamidino-2-phenylindole)-stained cells hybridized with the bacte...

journal_title：Applied and environmental microbiology

authors： Llobet-Brossa E,Rosselló-Mora R,Amann R

更新日期：1998-07-01 00:00:00

abstract：:Rhodococcus erythropolis N1-36, a desulfurization strain, was grown in continuous culture at 10 different dilution rates with 50 (mu)M dibenzothiophene sulfone (DBTO(inf2)) as the growth-limiting nutrient. The steady-state biomass, concentrations of substrate (DBTO(inf2)) and product (monohydroxybiphenyl), saturation ...

journal_title：Applied and environmental microbiology

authors： Wang P,Humphrey AE,Krawiec S

更新日期：1996-08-01 00:00:00

abstract：:Washed and air-oxidized proteins from Sporomusa ovata cleaved the C-O bond of methanol or methoxyaromatics and transferred the methyl to dl-tetrahydrofolate. The reactions strictly required a reductive activation by titanium citrate, catalytic amounts of ATP, and the addition of dl-tetrahydrofolate. Methylcorrinoid-co...

journal_title：Applied and environmental microbiology

authors： Stupperich E,Konle R

更新日期：1993-09-01 00:00:00

abstract：:We have developed a tool for controlled expression of heterologous or ectopic genes in the chestnut pathogen Cryphonectria parasitica using the promoter region from a putative copper-regulated transporter gene. In addition, we have found that expression control via this system is not affected by the virulence-attenuat...

journal_title：Applied and environmental microbiology

authors： Willyerd KL,Kemp AM,Dawe AL

更新日期：2009-08-01 00:00:00

abstract：:Acinetobacter baumannii is a metabolically versatile pathogen that causes severe infections in compromised patients. However, little is known about the genes and factors involved in its basic physiology and virulence properties. Insertion mutagenesis was used to initiate the identification and characterization of some...

journal_title：Applied and environmental microbiology

authors： Dorsey CW,Tomaras AP,Actis LA

更新日期：2002-12-01 00:00:00

abstract：:Storage of Salmonella enterica serovar Typhimurium strains in soil and water microcosms resulted in loss of culturability on standard plating media. Prior incubation in buffered peptone water supplemented with ferrioxamine E markedly extended the time that bacteria were recoverable by plating, except in the case of mu...

journal_title：Applied and environmental microbiology

authors： Reissbrodt R,Heier H,Tschäpe H,Kingsley RA,Williams PH

更新日期：2000-09-01 00:00:00

abstract：:The temperature of chilled foods is an important variable for controlling microbial growth in a production and distribution chain. Therefore, it is essential to model growth as a function of temperature in order to predict the number of organisms as a function of temperature and time. This article deals with the corre...

journal_title：Applied and environmental microbiology

authors： Zwietering MH,Cuppers HG,de Wit JC,van 't Riet K

更新日期：1994-01-01 00:00:00