Controlled gene expression in the plant pathogen Cryphonectria parasitica by use of a copper-responsive element.

abstract：:Poultry are considered a major reservoir and source of human campylobacteriosis, but the roles of environmental reservoirs, including wild birds, have not been assessed in depth. In this study, we isolated and characterized Campylobacter jejuni from western jackdaws (n = 91, 43%), mallard ducks (n = 82, 76%), and phea...

journal_title：Applied and environmental microbiology

authors： Kovanen S,Rossi M,Pohja-Mykrä M,Nieminen T,Raunio-Saarnisto M,Sauvala M,Fredriksson-Ahomaa M,Hänninen ML,Kivistö R

更新日期：2019-02-06 00:00:00

abstract：:The interactions of bacteria isolated from corroded copper coupons on thin films of copper evaporated onto germanium internal reflection elements were evaluated nondestructively in real time by attentuated total reflectance Fourier transform infrared spectroscopy. The films were stable in the presence of flowing or st...

journal_title：Applied and environmental microbiology

authors： Bremer PJ,Geesey GG

更新日期：1991-07-01 00:00:00

abstract：:Stepwise deletions in the only plasmid in Thermus thermophilus HB27, megaplasmid pTT27, showed that two distantly located loci were important for maintenance of the plasmid. One is a minimum replicon including one gene, repT, coding a replication initiator, and the other encodes subunits of class I ribonucleotide redu...

journal_title：Applied and environmental microbiology

authors： Ohtani N,Tomita M,Itaya M

更新日期：2015-12-28 00:00:00

abstract：:VanA-type human (n=69), animal (n=49), and food (n=36) glycopeptide-resistant enterococci (GRE) from different geographic areas were investigated to study their possible reservoirs and transmission routes. Pulsed-field gel electrophoresis (PFGE) revealed two small genetically related clusters, M39 (n=4) and M49 (n=13)...

journal_title：Applied and environmental microbiology

authors： Biavasco F,Foglia G,Paoletti C,Zandri G,Magi G,Guaglianone E,Sundsfjord A,Pruzzo C,Donelli G,Facinelli B

更新日期：2007-05-01 00:00:00

abstract：:The utilization by Alcaligenes faecalis of electrodes as the electron donor for denitrification was investigated in this study. The denitrification rate of A. faecalis with a poised potential was greatly enhanced compared with that of the controls without poised potentials. For nitrate reduction, although A. faecalis ...

journal_title：Applied and environmental microbiology

authors： Wang X,Yu P,Zeng C,Ding H,Li Y,Wang C,Lu A

更新日期：2015-08-15 00:00:00

abstract：:The determination of bacterial densities in aquatic sediments generally requires that a dilution-mixing treatment be used before enumeration of organisms by the most-probable-number fermentation tube method can be done. Differential sediment and organism settling rates may, however, influence the distribution of the m...

journal_title：Applied and environmental microbiology

authors： Doyle JD,Tunnicliff B,Kramer RE,Brickler SK

更新日期：1984-10-01 00:00:00

abstract：:Erwinia amylovora bacteriophages (phages) belonging to the Myoviridae and Podoviridae families demonstrated a preference for either high-exopolysaccharide-producing (HEP) or low-exopolysaccharide-producing (LEP) bacterial hosts when grown on artificial medium without or with sugar supplementation. Myoviridae phages pr...

journal_title：Applied and environmental microbiology

authors： Roach DR,Sjaarda DR,Castle AJ,Svircev AM

更新日期：2013-05-01 00:00:00

abstract：:An NAD(P)H-nicotine blue (quinone) oxidoreductase was discovered as a member of the nicotine catabolic pathway of Arthrobacter nicotinovorans. Transcriptional analysis and electromobility shift assays showed that the enzyme gene was expressed in a nicotine-dependent manner under the control of the transcriptional acti...

journal_title：Applied and environmental microbiology

authors： Mihasan M,Chiribau CB,Friedrich T,Artenie V,Brandsch R

更新日期：2007-04-01 00:00:00

abstract：:Ethanol caused altered mobility of the lipophilic probe 1,6-diphenyl-1,3,5-hexatriene in plasma membrane preparations of Saccharomyces cerevisiae. Because lipids had been shown to protect yeast cells against ethanol toxicity, sterols, fatty acids, proteins, and combinations of these were tested; however, protection fr...

journal_title：Applied and environmental microbiology

authors： Walker-Caprioglio HM,Rodriguez RJ,Parks LW

更新日期：1985-09-01 00:00:00

abstract：:A vineyard isolate of the yeast Saccharomyces cerevisiae, UCD932, was identified as a strain producing little or no detectable hydrogen sulfide during wine fermentation. Genetic analysis revealed that this trait segregated as a single genetic determinant. The gene also conferred a white colony phenotype on BiGGY agar ...

journal_title：Applied and environmental microbiology

authors： Linderholm A,Dietzel K,Hirst M,Bisson LF

更新日期：2010-12-01 00:00:00

abstract：:Analysis of a complete set of glutamate decarboxylase (gad) mutants of Listeria monocytogenes strain LO28 (ΔgadD1, ΔgadDT1, ΔgadD2, ΔgadT2, and ΔgadD3 mutants) revealed that the ΔgadD1 mutant is impaired in its ability to tolerate exposure to both sublethal and lethal levels of the lantibiotic nisin. gadD1 is strain v...

journal_title：Applied and environmental microbiology

authors： Begley M,Cotter PD,Hill C,Ross RP

更新日期：2010-10-01 00:00:00

abstract：:Thirty-nine isolates of fungi obtained from foodstuffs and soil samples from various parts of the world have been identified. The isolates were grown on a solid rice medium, and extracts were prepared with 50% aqueous methanol. The extracts were examined for toxicity in the following systems: (i) cytotoxicity to cultu...

journal_title：Applied and environmental microbiology

authors： Abbas HK,Mirocha CJ,Shier WT

更新日期：1984-09-01 00:00:00

abstract：:Lactobacillus isolates were identified by PCR amplification and sequencing of the region between the 16S and 23S rRNA genes (spacer region). The sequences obtained from the isolates were compared to those of reference strains held in GenBank. A similarity of 97.5% or greater was considered to provide identification. T...

journal_title：Applied and environmental microbiology

authors： Tannock GW,Tilsala-Timisjarvi A,Rodtong S,Ng J,Munro K,Alatossava T

更新日期：1999-09-01 00:00:00

abstract：:Methylosinus trichosporium OB3b produces an extracellular copper-binding ligand (CBL) with high affinity for copper. Wild-type cells and mutants that express soluble methane monooxygenase (sMMO) in the presence and absence of copper (sMMOc) were used to obtain cell exudates that were separated and analyzed by size exc...

journal_title：Applied and environmental microbiology

authors： Téllez CM,Gaus KP,Graham DW,Arnold RG,Guzman RZ

更新日期：1998-03-01 00:00:00

abstract：:Agrochemicals, fecal indicator bacteria (FIB), and pathogens frequently contaminate water simultaneously. No significant direct effects of fertilizer, atrazine, malathion, and chlorothalonil on the survival of Escherichia coli, Enterococcus faecalis, Salmonella enterica, human polyomaviruses, and adenovirus were detec...

journal_title：Applied and environmental microbiology

authors： Staley ZR,Senkbeil JK,Rohr JR,Harwood VJ

更新日期：2012-11-01 00:00:00

abstract：:In this work, the enzymatic activities of selected strains of biotypes 1 and 2 of Vabrio vulnificus were analyzed by using conventional methods and the API ZYM system. The toxic activities of extracellular products (ECPs) were further evaluated by in vitro and in vivo experiments. The ECPs of both biotypes (i) showed ...

journal_title：Applied and environmental microbiology

authors： Biosca EG,Amaro C

更新日期：1996-07-01 00:00:00

abstract：:The heat destruction characteristics of Clostridium botulinum spores suspended in tomato juice and phosphate buffer were determined by the survivor curve method with aluminum thermal death time tubes. Two type A strains of C. botulinum and a type B strain were evaluated. Strains A16037 and B15580 were implicated in ou...

journal_title：Applied and environmental microbiology

authors： Odlaug TE,Pflug IJ

更新日期：1977-07-01 00:00:00

abstract：:T1, a dentrifying bacterium originally isolated for its ability to grow on toluene, can also metabolize 4-hydroxybenzoate and other aromatic compounds under denitrifying conditions. A cosmid clone carrying the three genes that code for the 4-chlorobenzoate dehalogenase enzyme complex isolated from the aerobic bacteriu...

journal_title：Applied and environmental microbiology

authors： Coschigano PW,Häggblom MM,Young LY

更新日期：1994-03-01 00:00:00

abstract：:3-Hydroxypyridine (3HP) is an important natural pyridine derivative. Ensifer adhaerens HP1 can utilize 3HP as its sole sources of carbon, nitrogen, and energy to grow, but the genes responsible for the degradation of 3HP remain unknown. In this study, we predicted that a gene cluster, designated 3hpd, might be respons...

journal_title：Applied and environmental microbiology

authors： Wang H,Wang X,Ren H,Wang X,Lu Z

更新日期：2020-09-17 00:00:00

abstract：:Confocal scanning laser microscopy (CSLM) was used to demonstrate the attachment of Escherichia coli O157:H7 transformed with a plasmid encoding for green fluorescent protein (GFP) to the surface and within the internal structures of nonwaxed Red Delicious cv. apples. Apples at 2 or 25 degrees C were inoculated with a...

journal_title：Applied and environmental microbiology

authors： Burnett SL,Chen J,Beuchat LR

更新日期：2000-11-01 00:00:00

abstract：:We assayed the tolerance to solvents of three toluene-degrading Pseudomonas putida strains and Pseudomonas mendocina KR1 in liquid and soil systems. P. putida DOT-T1 tolerated concentrations of heptane, propylbenzene, octanol, and toluene of at least 10% (vol/vol), while P. putida F1 and EEZ15 grew well in the presenc...

journal_title：Applied and environmental microbiology

authors： Huertas MJ,Duque E,Marqués S,Ramos JL

更新日期：1998-01-01 00:00:00

abstract：:Despite efforts to minimize fecal input into waterways, this kind of pollution continues to be a problem due to an inability to reliably identify nonpoint sources. Our objective was to find candidate source-specific Escherichia coli fingerprints as potential genotypic markers for raw sewage, horses, dogs, gulls, and c...

journal_title：Applied and environmental microbiology

authors： Seurinck S,Verstraete W,Siciliano SD

更新日期：2003-08-01 00:00:00

abstract：:Current approaches used for dose-response modeling of low-dose exposures of pathogens rely on assumptions and extrapolations. These models are important for quantitative microbial risk assessment of food. A mechanistic framework has been advocated as an alternative approach for evaluating dose-response relationships. ...

journal_title：Applied and environmental microbiology

authors： Abe H,Koyama K,Koseki S

更新日期：2020-12-17 00:00:00

abstract：:A molecular method based on restriction fragment length polymorphism (RFLP) of PCR-amplified fragments of the 23S rRNA gene was designed to rapidly identify Listeria strains to the species level. Two fragments (S1, 460 bp, and S2, 890 bp) were amplified from boiled DNA. S2 was cut with the restriction enzymes XmnI or ...

journal_title：Applied and environmental microbiology

authors： Paillard D,Dubois V,Duran R,Nathier F,Guittet C,Caumette P,Quentin C

更新日期：2003-11-01 00:00:00

abstract：:Mortierella isabellina ATCC 38063 is a zygomycete capable of hydroxylating fish-toxic resin acids which occur in certain pulp mill effluents to nontoxic metabolites. Addition of dehydroabietic acid (1) (80 mg/liter) to a freshly inoculated culture of M. isabellina in dextrose-yeast extract broth resulted in precursor ...

journal_title：Applied and environmental microbiology

authors： Kutney JP,Choi LS,Hewitt GM,Salisbury PJ,Singh M

更新日期：1985-01-01 00:00:00

abstract：:Vibrio cholerae, an environmental organism, is a facultative human pathogen. Here, we report the virulence profiles, comprising 18 genetic markers, of 102 clinical and 692 environmental V. cholerae strains isolated in Bangladesh between March 2004 and January 2006, showing the variability of virulence determinants wit...

journal_title：Applied and environmental microbiology

authors： Hasan NA,Ceccarelli D,Grim CJ,Taviani E,Choi J,Sadique A,Alam M,Siddique AK,Sack RB,Huq A,Colwell RR

更新日期：2013-09-01 00:00:00

abstract：:Killer toxin activity of Pichia anomala WC65 appeared fungicidal for P. bimundalis WC38 and fungistatic for Candida albicans RC1. Inhibitory activity against sensitive C. albicans showed a linear relationship between toxin concentrations and the inverse of the reduced growth rates. The plot of toxin concentrations aga...

journal_title：Applied and environmental microbiology

authors： Sawant AD,Abdelal AT,Ahearn DG

更新日期：1988-05-01 00:00:00

abstract：:Glycoside hydrolase (GH) family 12 comprises enzymes with a wide range of activities critical for the degradation of lignocellulose. However, the important roles of the loop regions of GH12 enzymes in substrate specificity and catalytic efficiency remain poorly understood. This study examined how the loop 3 region aff...

journal_title：Applied and environmental microbiology

authors： Yang H,Shi P,Liu Y,Xia W,Wang X,Cao H,Ma R,Luo H,Bai Y,Yao B

更新日期：2017-03-02 00:00:00

abstract：:Four Staphylococcus aureus-Escherichia coli shuttle vectors were constructed for gene expression and production of tagged fusion proteins. Vectors pBUS1-HC and pTSSCm have no promoter upstream of the multiple cloning site (MCS), and this allows study of genes under the control of their native promoters, and pBUS1-Pcap...

journal_title：Applied and environmental microbiology

authors： Schwendener S,Perreten V

更新日期：2015-05-01 00:00:00

abstract：:Bacteria in the phylum Acidobacteria are widely distributed and abundant in soils, but their ecological roles are poorly understood, owing in part to a paucity of cultured representatives. In a molecular survey of acidobacterial diversity at the Michigan State University Kellogg Biological Station Long-Term Ecological...

journal_title：Applied and environmental microbiology

authors： Eichorst SA,Breznak JA,Schmidt TM

更新日期：2007-04-01 00:00:00