Biotransformation of dehydroabietic acid with resting cell suspensions and calcium alginate-immobilized cells of Mortierella isabellina.

Abstract:

:Mortierella isabellina ATCC 38063 is a zygomycete capable of hydroxylating fish-toxic resin acids which occur in certain pulp mill effluents to nontoxic metabolites. Addition of dehydroabietic acid (1) (80 mg/liter) to a freshly inoculated culture of M. isabellina in dextrose-yeast extract broth resulted in precursor disappearance in 28 to 30 h. During growth phase, hydroxylation occurred at C-2, whereas hydroxylation at C-15 and C-16 commenced with onset of stationary phase. Alternatively, 1 added to stationary-phase culture (40 mg/liter) disappeared within 2 h and hydroxylation occurred concurrently at C-2, C-15, and C-16. Enzymatic activity of stationary-phase culture was totally cell associated and was present despite the absence of 1 during the preparatory growth phase. Resuspension of mature fungi as free mycelia or immobilized in calcium alginate beads did not diminish the effectiveness of the biotransformation, although two new metabolites, 15-hydroxy-8,9,11,12-tetradehydro-7,8-dihydroabietic acid (5) and 16-hydroxy-8,9,11,12-tetradehydro-7,8-dihydroabietic acid (7) were formed. Immobilized mycelia retained hydroxylase activity for greater than 110 days whether or not they were challenged with fresh 1 on a regular basis. In this respect they are more long-lived than resuspended free mycelia are.

journal_name

Appl Environ Microbiol

authors

Kutney JP,Choi LS,Hewitt GM,Salisbury PJ,Singh M

doi

10.1128/AEM.49.1.96-100.1985

subject

Has Abstract

pub_date

1985-01-01 00:00:00

pages

96-100

issue

1

eissn

0099-2240

issn

1098-5336

journal_volume

49

pub_type

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