Abstract:
:We sequenced the Staphylococcus aureus phospho-beta-galactosidase gene. The protein product of this gene consisted of 470 amino acids, giving a molecular weight of 54,557. This gene appears to be transcribed as the terminal sequence on a polycistronic message.
journal_name
Appl Environ Microbioljournal_title
Applied and environmental microbiologyauthors
Breidt F Jr,Stewart GCdoi
10.1128/AEM.53.5.969-973.1987subject
Has Abstractpub_date
1987-05-01 00:00:00pages
969-73issue
5eissn
0099-2240issn
1098-5336journal_volume
53pub_type
杂志文章abstract::Fluorescent antibodies (FAs) prepared against 16 ammonia-oxidizing nitrifying bacteria were examined as to cross-reactivity in heterologous FA staining tests. Virtually all cross-reactions to the seven Nitrosomonas FAs were confined to the Nitrosomonas isolates. The five Nitrosospira, three Nitrosolobus, and one Nitro...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.36.4.589-593.1978
更新日期:1978-10-01 00:00:00
abstract::Magnetotactic bacteria comprise a phylogenetically diverse group that is capable of synthesizing intracellular magnetic particles. Although various morphotypes of magnetotactic bacteria have been observed in the environment, bacterial strains available in pure culture are currently limited to a few genera due to diffi...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.02124-09
更新日期:2010-03-01 00:00:00
abstract::Dehalococcoides sp. strain BAV1 couples growth with the reductive dechlorination of vinyl chloride (VC) to ethene. Degenerate primers targeting conserved regions in reductive dehalogenase (RDase) genes were designed and used to PCR amplify putative RDase genes from strain BAV1. Seven unique RDase gene fragments were i...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.70.10.6347-6351.2004
更新日期:2004-10-01 00:00:00
abstract::As a result of the recently proposed mandatory groundwater disinfection requirements to inactivate viruses in potable water supplies, there has been increasing interest in virus fate and transport in the subsurface. Several models have been developed to predict the fate of viruses in groundwater, but few include trans...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.58.5.1609-1616.1992
更新日期:1992-05-01 00:00:00
abstract::The reporter transposon-based system TnFuZ was used to identify exported proteins of the animal pathogen Corynebacterium pseudotuberculosis. Thirty-four out of 1,500 mutants had detectable alkaline phosphatase (PhoZ) activity. This activity was from 21 C. pseudotuberculosis loci that code for fimbrial and transport su...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.00294-06
更新日期:2006-11-01 00:00:00
abstract::Although the high level of competence for natural transformation of Acinetobacter sp. strain BD413 has been the subject of numerous studies, only two competence genes, comC and comP, have been identified to date. By chromosomal walking analysis we found two overlapping open reading frames, designated comE and comF, st...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.65.10.4568-4574.1999
更新日期:1999-10-01 00:00:00
abstract::Cold shock at 0 to 15 degrees C for 1 to 3 h increased the thermal sensitivity of Listeria monocytogenes. In a model broth system, thermal death time at 60 degrees C was reduced by up to 45% after L. monocytogenes Scott A was cold shocked for 3 h. The duration of the cold shock affected thermal tolerance more than did...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/aem.66.10.4345-4350.2000
更新日期:2000-10-01 00:00:00
abstract::Free-living and surface-associated microbial communities in sand-packed columns perfused with groundwater were compared by examination of compositional and functional characteristics. The composition of the microbial communities was assessed by bulk DNA extraction, PCR amplification of 16S ribosomal DNA fragments, sep...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/aem.68.4.1569-1575.2002
更新日期:2002-04-01 00:00:00
abstract::Ring-hydroxylating dioxygenases (RHDs) play a crucial role in the biodegradation of a range of aromatic hydrocarbons found on polluted sites, including polycyclic aromatic hydrocarbons (PAHs). Current knowledge on RHDs comes essentially from studies on culturable bacterial strains, while compelling evidence indicates ...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.01883-14
更新日期:2014-11-01 00:00:00
abstract::A beta-tubulin gene from a UV-irradiated benomyl-resistant mutant of Fusarium moniliforme was isolated, cloned, and sequenced. The gene encodes a 446-amino-acid polypeptide with homology to other fungal beta-tubulins. RNA blot analysis showed expression of the gene during vegetative growth and conidial germination but...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.62.8.3053-3056.1996
更新日期:1996-08-01 00:00:00
abstract::Novel strains of obligately chemolithoautotrophic, sulfur-oxidizing bacteria have been isolated from various depths of Lake Fryxell, Antarctica. Physiological, morphological, and phylogenetic analyses showed these strains to be related to mesophilic Thiobacillus species, such as T. thioparus. However, the psychrotoler...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.00702-06
更新日期:2006-08-01 00:00:00
abstract::Despite the benefits to the global food supply and agricultural economies, pesticides are believed to pose a threat to the health of both humans and wildlife. Chlorpyrifos (CP), a commonly used organophosphate insecticide, has poor target specificity and causes acute neurotoxicity in a wide range of species via the su...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.02820-17
更新日期:2018-04-16 00:00:00
abstract::We describe the stabilization by pressure of enzymes, including a hydrogenase from Methanococcus jannaschii, an extremely thermophilic deep-sea methanogen. This is the first published report of proteins from thermophiles being stabilized by pressure. Inactivation studies of partially purified hydrogenases from an extr...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.60.3.932-939.1994
更新日期:1994-03-01 00:00:00
abstract::Six strains of Clostridium thermocellum isolated from various environments were characterized as to growth rate, production of reducing sugars, ethanol, and acetic acid from cellulose, base composition of DNA, and the abilities to adapt to ethanol and to grow at 45 degrees C. Five of the six new isolates produced 7 to...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.49.3.475-477.1985
更新日期:1985-03-01 00:00:00
abstract::Nested PCR with three highly degenerate primers was used for amplification and identification of DNA polymerase (pol) genes from viruses which infect three genera of microalgae. Group-specific primers (AVS1 and AVS2) were designed on the basis of inferred amino acid sequences unique to the DNA pol genes of viruses (PB...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.61.4.1274-1278.1995
更新日期:1995-04-01 00:00:00
abstract::Analysis of food samples for E. coli O157:H7 using the standard U.S. Food and Drug Administration procedure is frequently complicated by overgrowth of nontarget microorganisms. A new procedure was developed for enrichment of enterohemorrhagic E. coli (EHEC) which utilizes exposure to pH 2.00 for 2 h. This procedure yi...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/aem.70.2.1226-1230.2004
更新日期:2004-02-01 00:00:00
abstract::Batch cultures of a lithotrophic Fe(II)-oxidizing bacterium, strain BrT, isolated from the rhizosphere of a wetland plant, were grown in bioreactors and used to determine the significance of microbial Fe(II) oxidation at circumneutral pH and to identify abiotic variables that affect the partitioning between microbial ...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/aem.68.8.3988-3995.2002
更新日期:2002-08-01 00:00:00
abstract::The transmission of Escherichia coli O157:H7 from manure-contaminated soil and irrigation water to lettuce plants was demonstrated using laser scanning confocal microscopy, epifluorescence microscopy, and recovery of viable cells from the inner tissues of plants. E. coli O157:H7 migrated to internal locations in plant...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/aem.68.1.397-400.2002
更新日期:2002-01-01 00:00:00
abstract::The maximum growth temperature, the optimal growth temperature, and the estimated normal physiological range for growth of Shewanella gelidimarina are functions of water activity (a(w)), which can be manipulated by changing the concentration of sodium chloride. The growth temperatures at the boundaries of the normal p...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/aem.66.6.2422-2429.2000
更新日期:2000-06-01 00:00:00
abstract::Ammonia monooxygenase of Nitrosomonas europaea catalyzes the oxidation of alkanes (up to C(8)) to alcohols and alkenes (up to C(5)) to epoxides and alcohols in the presence of ammonium ions. Straight-chain, N-terminal alkynes (up to C(10)) all exhibited a time-dependent inhibition of ammonia oxidation without effects ...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.54.12.3187-3190.1988
更新日期:1988-12-01 00:00:00
abstract::Proteolytic but chitinase-deficient microbial cultures were isolated from shrimp shell waste and characterized. The most efficient isolate was found to be a mixed culture consisting of two Bacillus licheniformis strains, which were first determined microscopically and physiologically. Molecular characterization was ca...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.00938-06
更新日期:2006-12-01 00:00:00
abstract::beta-Aminoglutaric acid, a nonprotein amino acid isomer of glutamic acid, was found in the free amino acid pool of a marine bacterium, Alteromonas luteoviolacea. It was also found in a mixed culture of fermenting bacteria enriched from an anoxic marine sediment. ...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.50.2.543-545.1985
更新日期:1985-08-01 00:00:00
abstract::Enterococcus mundtii CUGF08, a lactic acid bacterium isolated from alfalfa sprouts, was found to produce mundticin L, a new class IIa bacteriocin that has a high level of inhibitory activity against the genus Listeria. The plasmid-associated operons containing genes for the mundticin L precursor, the ATP binding casse...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.00752-09
更新日期:2009-09-01 00:00:00
abstract::Two duck farms in Hong Kong were examined monthly for 1 year for the occurrence and persistence of influenza viruses within the duck communities. The predominant virus in one community was H3N2, a virus antigenically related to the pandemic Hong Kong strain. This virus was isolated monthly throughout the year from fec...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.43.1.110-115.1982
更新日期:1982-01-01 00:00:00
abstract::The vertical and seasonal distribution and diversity of archaeal sequences was investigated in a hypersaline, stratified, monomictic lake, Solar Lake, Sinai, Egypt, during the limnological development of stratification and mixing. Archaeal sequences were studied via phylogenetic analysis of 16S rDNA sequences as well ...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/aem.66.8.3269-3276.2000
更新日期:2000-08-01 00:00:00
abstract::Here we describe novel forms of structural integration between endo- and episymbiotic microbes and an unusual new species of snail from hydrothermal vents in the Indian Ocean. The snail houses a dense population of gamma-proteobacteria within the cells of its greatly enlarged esophageal gland. This tissue setting diff...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/aem.70.5.3082-3090.2004
更新日期:2004-05-01 00:00:00
abstract::A physiological, unbalanced model is presented that explicitly describes growth of the marine cyanobacterium Trichodesmium sp. at the expense of N(2) (diazotrophy). The model involves the dynamics of intracellular reserves of carbon and nitrogen and allows the uncoupling of the metabolism of these elements. The result...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.72.5.3217-3227.2006
更新日期:2006-05-01 00:00:00
abstract::DNA fingerprinting and molecular characterization showed that the tetracycline-resistant Staphylococcus aureus population of a South African poultry processing plant comprised one or possibly several tet(K)-containing endemic clones that contaminated chicken and machinery surfaces at all sampled processing stages. The...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.71.1.574-579.2005
更新日期:2005-01-01 00:00:00
abstract::Ergosterol is a membrane component specific to fungi that can be used to estimate fungal biomass using appropriate factors of conversion. Our objectives were to determine the limits of use of ergosterol content as a measure of biomass for aquatic hyphomycetes, and to evaluate a previously established ergosterol-to-bio...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.67.5.2051-2055.2001
更新日期:2001-05-01 00:00:00
abstract::Escherichia coli is being developed as a biocatalyst for bulk chemical production from inexpensive carbohydrates derived from lignocellulose. Potential substrates include the soluble xylodextrins (xyloside, xylooligosaccharide) and xylobiose that are produced by treatments designed to expose cellulose for subsequent e...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/aem.69.10.5957-5967.2003
更新日期:2003-10-01 00:00:00