Abstract:
:We describe the stabilization by pressure of enzymes, including a hydrogenase from Methanococcus jannaschii, an extremely thermophilic deep-sea methanogen. This is the first published report of proteins from thermophiles being stabilized by pressure. Inactivation studies of partially purified hydrogenases from an extreme thermophile (Methanococcus igneus), a moderate thermophile (Methanococcus thermolithotrophicus), and a mesophile (Methanococcus maripaludis), all from shallow marine sites, show that pressure stabilization is not unique to enzymes isolated from high-pressure environments. These studies suggest that pressure stabilization of an enzyme may be related to its thermophilicity. Further experiments comparing the effects of increased pressure on the stability of alpha-glucosidases from the hyperthermophile Pyrococcus furiosus and Saccharomyces cerevisiae support this possibility. We have also examined pressure effects on several highly homologous glyceraldehyde-3-phosphate dehydrogenases from mesophilic and thermophilic sources and a rubredoxin from P. furiosus. The results suggest that hydrophobic interactions, which have been implicated in the stabilization of many thermophilic proteins, contribute to the pressure stabilization of enzymes from thermophiles.
journal_name
Appl Environ Microbioljournal_title
Applied and environmental microbiologyauthors
Hei DJ,Clark DSdoi
10.1128/AEM.60.3.932-939.1994keywords:
subject
Has Abstractpub_date
1994-03-01 00:00:00pages
932-9issue
3eissn
0099-2240issn
1098-5336journal_volume
60pub_type
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更新日期:2006-01-01 00:00:00
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pub_type: 杂志文章
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更新日期:1982-07-01 00:00:00
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journal_title:Applied and environmental microbiology
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doi:10.1128/AEM.33.3.577-584.1977
更新日期:1977-03-01 00:00:00
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pub_type: 杂志文章
doi:10.1128/AEM.39.5.1078-1080.1980
更新日期:1980-05-01 00:00:00
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pub_type: 杂志文章
doi:10.1128/AEM.55.3.733-740.1989
更新日期:1989-03-01 00:00:00
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journal_title:Applied and environmental microbiology
pub_type: 杂志文章
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更新日期:1985-05-01 00:00:00
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journal_title:Applied and environmental microbiology
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更新日期:2004-03-01 00:00:00
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pub_type: 杂志文章
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更新日期:1993-09-01 00:00:00
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pub_type: 杂志文章
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journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.59.12.4216-4222.1993
更新日期:1993-12-01 00:00:00
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journal_title:Applied and environmental microbiology
pub_type: 杂志文章
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更新日期:1986-09-01 00:00:00
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journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.65.2.404-408.1999
更新日期:1999-02-01 00:00:00