Isolation and Characterization of Vacuoles from the Ergot Fungus Claviceps purpurea.

abstract：:Filamentous, gliding, sulfide-oxidizing bacteria of the genus Thioploca were found on sediments in profundal areas of Lake Biwa, a Japanese freshwater mesotrophic lake, and were characterized morphologically and phylogenetically. The Lake Biwa Thioploca resembled morphologically Thioploca ingrica, a brackish water spe...

journal_title：Applied and environmental microbiology

authors： Kojima H,Teske A,Fukui M

更新日期：2003-01-01 00:00:00

abstract：:Addition of sodium isoascorbate to the formulation for perishable canned comminuted cured meat markedly enhanced the efficacy of nitrite against Clostridium botulinum. This effect was reproducible through a series of three tests. In one test it was found that the initial addition of 50 microgram of sodium nitrite per ...

journal_title：Applied and environmental microbiology

authors： Tompkin RB,Christiansen LN,Shaparis AB

更新日期：1978-01-01 00:00:00

abstract：:Cytochrome c(3) from Desulfovibrio vulgaris Miyazaki F was successfully expressed in the facultative aerobe Shewanella oneidensis MR-1 under anaerobic, microaerophilic, and aerobic conditions, with yields of 0.3 to 0.5 mg of cytochrome/g of cells. A derivative of the broad-host-range plasmid pRK415 containing the cyto...

journal_title：Applied and environmental microbiology

authors： Ozawa K,Tsapin AI,Nealson KH,Cusanovich MA,Akutsu H

更新日期：2000-09-01 00:00:00

abstract：:Biochemical and proteomic tools have been utilized for investigating the mechanism of action of a new Stenotrophomonas maltophilia strain (SeITE02), a gammaproteobacterium capable of resistance to high concentrations of selenite [SeO(3)(2-), Se(IV)], reducing it to nontoxic elemental selenium under aerobic conditions;...

journal_title：Applied and environmental microbiology

authors： Antonioli P,Lampis S,Chesini I,Vallini G,Rinalducci S,Zolla L,Righetti PG

更新日期：2007-11-01 00:00:00

abstract：:Production of bacteriocin activity designated pediocin PA-1 was associated with the presence of a 6.2-megadalton plasmid in Pediococcus acidilactici PAC1.0. The bacteriocin exhibited activity against P. acidilactici, P. pentosaceus, Lactobacillus plantarum, L. casei, L. bifermentans, and Leuconostoc mesenteroides subs...

journal_title：Applied and environmental microbiology

authors： Gonzalez CF,Kunka BS

更新日期：1987-10-01 00:00:00

abstract：:Protein disulfide isomerase (PDI) is important in assisting the folding and maturation of secretory proteins in eukaryotes. A gene, pdiA, encoding PDIA was previously isolated from Aspergillus niger, and we report its functional characterization here. Functional analysis of PDIA showed that it catalyzes the refolding ...

journal_title：Applied and environmental microbiology

authors： Ngiam C,Jeenes DJ,Punt PJ,Van Den Hondel CA,Archer DB

更新日期：2000-02-01 00:00:00

abstract：:The restriction endonuclease Cac824I has been shown to be a major barrier to electrotransformation of Clostridium acetobutylicum ATCC 824 (L. D. Mermelstein, N. E. Welker, G. N. Bennett, and E. T. Papoutsakis, Bio/Technology 10:190-195, 1992). Methylation by the phi 3T I methyltransferase encoded by Bacillus subtilis ...

journal_title：Applied and environmental microbiology

authors： Mermelstein LD,Papoutsakis ET

更新日期：1993-04-01 00:00:00

abstract：:The Cry2Aa3 gene was introduced into asporogenic Bacillus thuringiensis, and the synthesized protoxin killed Bombyx mori and Lymantria dispar larvae. Chymotrypsin hydrolyzed the linkages between 49Tyr/Val50 and 145Lys/Ser146 in the protoxin, and 50- and 58-kDa fragments were generated, respectively. Both peptides kill...

journal_title：Applied and environmental microbiology

authors： Ohsawa M,Tanaka M,Moriyama K,Shimazu M,Asano S,Miyamoto K,Haginoya K,Mitsui T,Kouya T,Taniguchi M,Hori H

更新日期：2012-07-01 00:00:00

abstract：:The common reed (Phragmites australis), a cosmopolitan aquatic macrophyte, plays an important role in the structure and function of aquatic ecosystems. We compared bacterial community compositions (BCCs) and their assembly processes in the root-associated compartments (i.e., rhizosphere and endosphere) of reed and bul...

journal_title：Applied and environmental microbiology

authors： He R,Zeng J,Zhao D,Huang R,Yu Z,Wu QL

更新日期：2020-07-02 00:00:00

abstract：:Cells of a newly isolated environmental strain of Candida humicola accumulated 10-fold more polyphosphate (polyP), during active growth, when grown in complete glucose-mineral salts medium at pH 5.5 than when grown at pH 7.5. Neither phosphate starvation, nutrient limitation, nor anaerobiosis was required to induce po...

journal_title：Applied and environmental microbiology

authors： McGrath JW,Quinn JP

更新日期：2000-09-01 00:00:00

abstract：:An extracellular serine protease produced by a mutant, M1, derived from Streptomyces albogriseolus S-3253 that no longer produces a protease inhibitor (Streptomyces subtilisin inhibitor [SSI]) was isolated. A 20-kDa protein was purified by its affinity for SSI and designated SAM-P20. The amino acid sequence of the ami...

journal_title：Applied and environmental microbiology

authors： Taguchi S,Odaka A,Watanabe Y,Momose H

更新日期：1995-01-01 00:00:00

abstract：:Enteric microbiota play a variety of roles in intestinal health and disease. While bacteria in the intestine have been broadly characterized, little is known about the abundance or diversity of enteric fungi. This study utilized a culture-independent method termed oligonucleotide fingerprinting of rRNA genes (OFRG) to...

journal_title：Applied and environmental microbiology

authors： Scupham AJ,Presley LL,Wei B,Bent E,Griffith N,McPherson M,Zhu F,Oluwadara O,Rao N,Braun J,Borneman J

更新日期：2006-01-01 00:00:00

abstract：:Perchlorate is a known health hazard for humans, fish, and other species. Therefore, it is important to assess the response of an ecosystem exposed to perchlorate contamination. The data reported here show that a liquid chromatography-mass spectrometry-based proteomics approach for the detection of perchlorate-reducin...

journal_title：Applied and environmental microbiology

authors： Bansal R,Crawford RL,Paszczynski AJ

更新日期：2011-02-01 00:00:00

abstract：:The moderately halophilic bacterium Halobacillus halophilus carries a homologue of LuxS, a protein involved in the activated methyl cycle and the production of autoinducer-2, which mediates quorum sensing between certain species. luxS of H. halophilus is part of an operon that encodes an S-adenosylmethionine-dependent...

journal_title：Applied and environmental microbiology

authors： Sewald X,Saum SH,Palm P,Pfeiffer F,Oesterhelt D,Müller V

更新日期：2007-01-01 00:00:00

abstract：:A 1.5-kb region immediately downstream of the styABCD operon involved in styrene degradation in Pseudomonas putida CA-3 has been cloned. Sequence analysis revealed a 1,296-bp open reading frame, designated styE, and BLAST P database comparisons of the deduced StyE amino acid sequence revealed 33 to 98% identity with s...

journal_title：Applied and environmental microbiology

authors： Mooney A,O'Leary ND,Dobson AD

更新日期：2006-02-01 00:00:00

abstract：:The harvesting of rainwater is gaining acceptance among many governmental authorities in countries such as Australia, Germany, and South Africa, among others. However, conflicting reports on the microbial quality of harvested rainwater have been published. To monitor the presence of potential pathogenic bacteria durin...

journal_title：Applied and environmental microbiology

authors： Dobrowsky PH,De Kwaadsteniet M,Cloete TE,Khan W

更新日期：2014-04-01 00:00:00

abstract：:Two hybrid bacteriocins, enterocin E50-52/pediocin PA-1 (EP) and pediocin PA-1/enterocin E50-52 (PE), were designed by combining the N terminus of enterocin E50-52 and the C terminus of pediocin PA-1 and by combining the C terminus of pediocin PA-1 and the N terminus of enterocin E50-52, respectively. Both hybrid bact...

journal_title：Applied and environmental microbiology

authors： Tiwari SK,Sutyak Noll K,Cavera VL,Chikindas ML

更新日期：2015-03-01 00:00:00

abstract：:Enterotoxin-positive (Ent+) and enterotoxin-negative (Ent-) strains of Clostridium perfringens were cultured in Duncan-Strong sporulation medium containing starch at 37 and 46 degrees C. At 37 degrees C, all strains degraded starch and sporulated well. However, only Ent- strains could hydrolyze starch, grow extensivel...

journal_title：Applied and environmental microbiology

authors： García-Alvarado JS,Rodriguez MA,Labbé RG

更新日期：1992-01-01 00:00:00

abstract：:IncQ marker plasmids were previously constructed to enable the analysis of the survival of populations of Pseudomonas putida released into lake water (C. Winstanley, J. A. W. Morgan, R. W. Pickup, J. G. Jones, and J. R. Saunders, Appl. Environ. Microbiol. 55:771-777, 1989). We constructed equivalent IncP plasmids, pLV...

journal_title：Applied and environmental microbiology

authors： Winstanley C,Morgan JA,Pickup RW,Saunders JR

更新日期：1991-07-01 00:00:00

abstract：:Symbioses with gut microorganisms provides a means by which terrestrial herbivores are able to obtain energy. These microorganisms ferment cell wall materials of plants to short-chain fatty acids (SCFA), which are then absorbed and used by the host animal. Many marine herbivorous fishes contain SCFA (predominantly ace...

journal_title：Applied and environmental microbiology

authors： Mountfort DO,Campbell J,Clements KD

更新日期：2002-03-01 00:00:00

abstract：:Pesticides are key stressors of soil microorganisms with reciprocal effects on ecosystem functioning. These effects have been mainly attributed to the parent compounds, while the impact of their transformation products (TPs) has been largely overlooked. We assessed in a meadow soil (soil A) the transformation of iprod...

journal_title：Applied and environmental microbiology

authors： Vasileiadis S,Puglisi E,Papadopoulou ES,Pertile G,Suciu N,Pappolla RA,Tourna M,Karas PA,Papadimitriou F,Kasiotakis A,Ipsilanti N,Ferrarini A,Sułowicz S,Fornasier F,Menkissoglu-Spiroudi U,Nicol GW,Trevisan M,Karpouzas DG

更新日期：2018-10-30 00:00:00

abstract：:The Tinto River (Huelva, southwestern Spain) is an extreme environment with a rather constant acidic pH along the entire river and a high concentration of heavy metals. The extreme conditions of the Tinto ecosystem are generated by the metabolic activity of chemolithotrophic microorganisms thriving in the rich complex...

journal_title：Applied and environmental microbiology

authors： González-Toril E,Llobet-Brossa E,Casamayor EO,Amann R,Amils R

更新日期：2003-08-01 00:00:00

abstract：:Streptomyces exfoliatus SMF19 produced two types of extracellular beta-lactamase-inhibiting proteins, 48 (beta LIP-I) and 33 (beta LIP-II) kDa. The inhibition mode of beta LIP-I on Bacto Penase and benzylpenicillin as substrates was uncompetitive (i.e., both Km and Vmax were changed), while that of beta LIP-II was non...

journal_title：Applied and environmental microbiology

authors： Kim MK,Lee KJ

更新日期：1994-03-01 00:00:00

abstract：:Isolates of the obligately biotrophic fungus Uncinula necator cluster in three distinct genetic groups (groups I, II, and III). We designed PCR primers specific for these groups in order to monitor field populations of U. necator. We used the nucleotide sequences of the gene that encodes eburicol 14alpha-demethylase (...

journal_title：Applied and environmental microbiology

authors： Délye C,Ronchi V,Laigret F,Corio-Costet MF

更新日期：1999-09-01 00:00:00

abstract：:Due to concerns about a link between variant Creutzfeldt-Jakob disease in humans and similar prion protein-induced disease in cattle, i.e., bovine spongiform encephalopathy (BSE), strict controls are in place to exclude BSE-positive animals and/or specified risk materials including bovine central nervous system (CNS) ...

journal_title：Applied and environmental microbiology

authors： Daly DJ,Prendergast DM,Sheridan JJ,Blair IS,McDowell DA

更新日期：2002-02-01 00:00:00

abstract：:Salmonella enterica serovar Enteritidis strain E40 filaments were developed under conditions of a reduced water activity (a(w)) of 0.95 in tryptic soy broth (TSB) or tryptic soy agar (TSA) supplemented with 8% or 7% NaCl, respectively. Filament formation was accompanied by an increase of biomass without an increase in...

journal_title：Applied and environmental microbiology

authors： Stackhouse RR,Faith NG,Kaspar CW,Czuprynski CJ,Wong AC

更新日期：2012-04-01 00:00:00

abstract：:High-throughput sequencing of the taxonomically informative 16S rRNA gene provides a powerful approach for exploring microbial diversity. Here we compare the performances of two common "benchtop" sequencing platforms, Illumina MiSeq and Ion Torrent Personal Genome Machine (PGM), for bacterial community profiling by 16...

journal_title：Applied and environmental microbiology

authors： Salipante SJ,Kawashima T,Rosenthal C,Hoogestraat DR,Cummings LA,Sengupta DJ,Harkins TT,Cookson BT,Hoffman NG

更新日期：2014-12-01 00:00:00

abstract：:A 15-mer peptide fragment derived from pediocin PA-1 (from residue 20 to residue 34) specifically inhibited the bactericidal activity of pediocin PA-1. The fragment did not inhibit the pediocin-like bacteriocins sakacin P, leucocin A, and curvacin A to nearly the same extent as it inhibited pediocin PA-1. Enterocin A,...

journal_title：Applied and environmental microbiology

authors： Fimland G,Jack R,Jung G,Nes IF,Nissen-Meyer J

更新日期：1998-12-01 00:00:00

abstract：:Celiac disease (CD) is an immune-mediated enteropathy triggered by the ingestion of cereal gluten proteins. This disorder is associated with imbalances in the gut microbiota composition that could be involved in the pathogenesis of CD. The aim of this study was to characterize the composition and diversity of the cult...

journal_title：Applied and environmental microbiology

authors： Sánchez E,Donat E,Ribes-Koninckx C,Fernández-Murga ML,Sanz Y

更新日期：2013-09-01 00:00:00

abstract：:Microbial transformation of lapachol, a naturally occurring naphthoquinone, was carried out by Curvularia lunata (NRRL 2178). The fungus brings about oxidative cyclization of the substrate to dehydro-alpha-lapachone, which was isolated and characterized by nuclear magnetic resonance and mass spectral analyses; its str...

journal_title：Applied and environmental microbiology

authors： Otten S,Rosazza JP

更新日期：1979-08-01 00:00:00