Abstract:
:The moderately halophilic bacterium Halobacillus halophilus carries a homologue of LuxS, a protein involved in the activated methyl cycle and the production of autoinducer-2, which mediates quorum sensing between certain species. luxS of H. halophilus is part of an operon that encodes an S-adenosylmethionine-dependent methyltransferase, a cysteine synthase, and a beta-cystathionine lyase. Expression of luxS was growth phase dependent, with maximal expression in the mid-exponential growth phase. In addition, transcription of luxS was strictly salt dependent; maximal mRNA concentrations were observed with 2.0 M NaCl in the growth medium. Chloride ions stimulated luxS transcription by a factor of three. Western blot analyses demonstrated a growth phase- and salinity-dependent production of LuxS. Moreover, cellular LuxS levels were strictly chloride dependent. Maximal accumulation of LuxS was observed at 0.5 to 1.0 M Cl(-) and depended on the salinity.
journal_name
Appl Environ Microbioljournal_title
Applied and environmental microbiologyauthors
Sewald X,Saum SH,Palm P,Pfeiffer F,Oesterhelt D,Müller Vdoi
10.1128/AEM.01625-06subject
Has Abstractpub_date
2007-01-01 00:00:00pages
371-9issue
2eissn
0099-2240issn
1098-5336pii
AEM.01625-06journal_volume
73pub_type
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