Marker rescue studies of the transfer of recombinant DNA to Streptococcus gordonii in vitro, in foods and gnotobiotic rats.

abstract：:Mesophilic and psychrotrophic populations from refrigerated meat were identified in this study, and the spoilage potential of microbial isolates in packaged beef was evaluated by analyzing the release of volatile organic compounds (VOC) by gas chromatography-mass spectrometry (GC/MS). Fifty mesophilic and twenty-nine ...

journal_title：Applied and environmental microbiology

authors： Ercolini D,Russo F,Nasi A,Ferranti P,Villani F

更新日期：2009-04-01 00:00:00

abstract：:It has previously been shown that the tomato pathogen Clavibacter michiganensis subsp. michiganensis secretes a 14-kDa protein, C. michiganensis subsp. michiganensis AMP-I (CmmAMP-I), that inhibits growth of Clavibacter michiganensis subsp. sepedonicus, the causal agent of bacterial ring rot of potato. Using sequences...

journal_title：Applied and environmental microbiology

authors： Liu Z,Ma P,Holtsmark I,Skaugen M,Eijsink VG,Brurberg MB

更新日期：2013-09-01 00:00:00

abstract：:Atypical El Tor strains of Vibrio cholerae O1 harboring variant ctxB genes of cholera toxin (CT) have gradually become a major cause of recent cholera epidemics. Vibrio mimicus occasionally produces CT, encoded by ctxAB on CTXФ genome; toxin-coregulated pilus (TCP), a major intestinal colonization factor; and also the...

journal_title：Applied and environmental microbiology

authors： Neogi SB,Chowdhury N,Awasthi SP,Asakura M,Okuno K,Mahmud ZH,Islam MS,Hinenoya A,Nair GB,Yamasaki S

更新日期：2019-01-23 00:00:00

abstract：:The effects of organic and inorganic nutrient additions on the specific growth rates of bacterioplankton in oligotrophic lake water cultures were investigated. Lake water was first passed through 0.8-mum-pore-size filters (prescreening) to remove bacterivores and to minimize confounding effects of algae. Specific grow...

journal_title：Applied and environmental microbiology

authors： Coveney MF,Wetzel RG

更新日期：1992-01-01 00:00:00

abstract：:The physiology of filamentous fungi at growth rates approaching zero has been subject to limited study and exploitation. With the aim of uncoupling product formation from growth, we have revisited and improved the retentostat cultivation method for Aspergillus niger. A new retention device was designed allowing reliab...

journal_title：Applied and environmental microbiology

authors： Jørgensen TR,Nitsche BM,Lamers GE,Arentshorst M,van den Hondel CA,Ram AF

更新日期：2010-08-01 00:00:00

abstract：:The N-chloramine compound 3-chloro-4,4-dimethyl-2-oxazolidinone (agent I) has been compared with calcium hypochlorite as to its efficacy as a bactericide for the treatment of water. The study included concentration, contact time, pH, temperature, and water quality as controlled variables. The species of bacteria teste...

journal_title：Applied and environmental microbiology

authors： Williams DE,Worley SD,Wheatley WB,Swango LJ

更新日期：1985-03-01 00:00:00

abstract：:A cattle trial using artificially inoculated calves was conducted to determine the effect of the addition of colicinogenic Escherichia coli strains capable of producing colicin E7 (a 61-kDa DNase) to feed on the fecal shedding of serotype O157:H7. The experiment was divided into three periods. In period 1, which laste...

journal_title：Applied and environmental microbiology

authors： Schamberger GP,Phillips RL,Jacobs JL,Diez-Gonzalez F

更新日期：2004-10-01 00:00:00

abstract：:Biodesulfurization was monitored in a recombinant Pseudomonas putida CECT5279 strain. DszB desulfinase activity reached a sharp maximum at the early exponential phase, but it rapidly decreased at later growth phases. A model two-step resting-cell process combining sequentially P. putida cells from the late and early e...

journal_title：Applied and environmental microbiology

authors： Calzada J,Zamarro MT,Alcón A,Santos VE,Díaz E,García JL,Garcia-Ochoa F

更新日期：2009-02-01 00:00:00

abstract：:The beta-xylosidase-encoding xlnD gene of Aspergillus niger 90196 was amplified by the PCR technique from first-strand cDNA synthesized on mRNA isolated from the fungus. The nucleotide sequence of the cDNA fragment was verified to contain a 2,412-bp open reading frame that encodes a 804-amino-acid propeptide. The 778-...

journal_title：Applied and environmental microbiology

authors： La Grange DC,Pretorius IS,Claeyssens M,van Zyl WH

更新日期：2001-12-01 00:00:00

abstract：:Upon exposure of Nitrosomonas europaea to chloroform (7 microM, 1 h), transcripts for 175 of 2,460 genes were found at higher levels in treated cells than in untreated cells and transcripts for 501 genes were found at lower levels. With chloromethane (3.2 mM, 1 h), transcripts for 67 genes were at higher levels and tr...

journal_title：Applied and environmental microbiology

authors： Gvakharia BO,Permina EA,Gelfand MS,Bottomley PJ,Sayavedra-Soto LA,Arp DJ

更新日期：2007-05-01 00:00:00

abstract：:Through use of commercially available DnaK proteins and anti-DnaK monoclonal antibodies, a competitive enzyme-linked immunosorbent assay was developed to quantify this heat shock protein in Escherichia coli ATCC 25922 subjected to various heating regimens. For a given process lethality (F(70)(10) of 1, 3, and 5 min), ...

journal_title：Applied and environmental microbiology

authors： Seyer K,Lessard M,Piette G,Lacroix M,Saucier L

更新日期：2003-06-01 00:00:00

abstract：:The presence of PCR inhibitors in water samples is well known and contributes to the fact that a practical PCR assay has not been developed for legionella surveillance. In this study, we devised a new seminested PCR assay for detection of Legionella spp. in water samples as a means of overriding the PCR inhibitors wit...

journal_title：Applied and environmental microbiology

authors： Miyamoto H,Yamamoto H,Arima K,Fujii J,Maruta K,Izu K,Shiomori T,Yoshida S

更新日期：1997-07-01 00:00:00

abstract：:An obligately anaerobic bacterium which transforms several chlorinated phenols was isolated. Dechlorination of the substituents ortho to the phenolic OH group was preferred, while removal of a meta-substituted chlorine was observed only with 3,5-dichlorophenol. The bacterium was a gram-positive, endospore-forming, mot...

journal_title：Applied and environmental microbiology

authors： Madsen T,Licht D

更新日期：1992-09-01 00:00:00

abstract：:Nontoxigenic strains of Clostridium botulinum types C and D are converted to toxigenic strains by infection with specific Tox+ bacteriophages. The nucleic acids were extracted from five converting phages, c-st, c-468, c-203, c-d6f, and d-1873, and one nonconverting phage, c-n71, and treated with nucleases. The nucleic...

journal_title：Applied and environmental microbiology

authors： Fujii N,Oguma K,Yokosawa N,Kimura K,Tsuzuki K

更新日期：1988-01-01 00:00:00

abstract：:A sedimentary bacterial isolate has been shown to contain trans-monounsaturated fatty acids (6% of the total fatty acids). The ratio of trans- to cis-acids in this isolate was in the range 3.2 to 7.6. The identification of trans-monounsaturated acids in a marine bacterium implied that the trans-acids which have been r...

journal_title：Applied and environmental microbiology

authors： Gillan FT,Johns RB,Verheyen TV,Volkman JK,Bavor HJ

更新日期：1981-04-01 00:00:00

abstract：:A screen of the Saccharomyces cerevisiae deletion strain set was performed to identify genes affecting hydrogen sulfide (H(2)S) production. Mutants were screened using two assays: colony color on BiGGY agar, which detects the basal level of sulfite reductase activity, and production of H(2)S in a synthetic juice mediu...

journal_title：Applied and environmental microbiology

authors： Linderholm AL,Findleton CL,Kumar G,Hong Y,Bisson LF

更新日期：2008-03-01 00:00:00

abstract：:Soils contain a tangle of minerals, water, nutrients, gases, plant roots, decaying organic matter, and microorganisms which work together to cycle nutrients and support terrestrial plant growth. Most soil microorganisms live in periodically interconnected communities closely associated with soil aggregates, i.e., smal...

journal_title：Applied and environmental microbiology

authors： Wilpiszeski RL,Aufrecht JA,Retterer ST,Sullivan MB,Graham DE,Pierce EM,Zablocki OD,Palumbo AV,Elias DA

更新日期：2019-07-01 00:00:00

abstract：:The Chloroflexi CL500-11 clade contributes a large proportion of the bacterial biomass in the oxygenated hypolimnia of deep lakes worldwide, including the world's largest freshwater system, the Laurentian Great Lakes. Traits that allow CL500-11 to thrive and its biogeochemical role in these environments are currently ...

journal_title：Applied and environmental microbiology

authors： Denef VJ,Mueller RS,Chiang E,Liebig JR,Vanderploeg HA

更新日期：2015-12-18 00:00:00

abstract：:Stored sugarbeet roots began fermenting within 24 h after oxygen was depleted at 26 C when the resident bacterial populations increased dramatically. Most of the bacteria present after anaerobic storage for 7 days at 26 C could hydrolyze sucrose in vitro. Although pH and sucrose levels decreased and invert sugar level...

journal_title：Applied and environmental microbiology

authors： Cole DF,Bugbee WM

更新日期：1976-05-01 00:00:00

abstract：:Kinetic parameters and the role of cytochrome c(3) in sulfate, Fe(III), and U(VI) reduction were investigated in Desulfovibrio vulgaris Hildenborough. While sulfate reduction followed Michaelis-Menten kinetics (K(m) = 220 micro M), loss of Fe(III) and U(VI) was first-order at all concentrations tested. Initial reducti...

journal_title：Applied and environmental microbiology

authors： Elias DA,Suflita JM,McInerney MJ,Krumholz LR

更新日期：2004-01-01 00:00:00

abstract：:Metabolic inhibitors were added to a culture medium inoculated with theramlly stressed Vibrio parahaemolyticus to obtain information pertaining to biosynthetic processes required for recovery from heat damage. Ribonucleic acid and protein syntheses, in addition to membrane repair, were required during recovery of inju...

journal_title：Applied and environmental microbiology

authors： Heinis JJ,Beuchat LR,Boswell FC

更新日期：1978-06-01 00:00:00

abstract：:A 15-mer peptide fragment derived from pediocin PA-1 (from residue 20 to residue 34) specifically inhibited the bactericidal activity of pediocin PA-1. The fragment did not inhibit the pediocin-like bacteriocins sakacin P, leucocin A, and curvacin A to nearly the same extent as it inhibited pediocin PA-1. Enterocin A,...

journal_title：Applied and environmental microbiology

authors： Fimland G,Jack R,Jung G,Nes IF,Nissen-Meyer J

更新日期：1998-12-01 00:00:00

abstract：:Piriformospora indica (Hymenomycetes, Basidiomycota) is a newly described cultivable endophyte that colonizes roots. Inoculation with the fungus and application of fungal culture filtrate promotes plant growth and biomass production. Due to its ease of culture, this fungus provides a model organism for the study of be...

journal_title：Applied and environmental microbiology

authors： Varma A,Savita Verma,Sudha,Sahay N,Butehorn B,Franken P

更新日期：1999-06-01 00:00:00

abstract：:A molecular method based on restriction fragment length polymorphism (RFLP) of PCR-amplified fragments of the 23S rRNA gene was designed to rapidly identify Listeria strains to the species level. Two fragments (S1, 460 bp, and S2, 890 bp) were amplified from boiled DNA. S2 was cut with the restriction enzymes XmnI or ...

journal_title：Applied and environmental microbiology

authors： Paillard D,Dubois V,Duran R,Nathier F,Guittet C,Caumette P,Quentin C

更新日期：2003-11-01 00:00:00

abstract：:Conditions for detection and quantitation of Clostridium perfringens enterotoxin by counterimmunoelectrophoresis are described. As little as 0.2 microgram of enterotoxin per ml could be detected. The test was found to be rapid, sensitive, specific and easy for the detection and quantitation of enterotoxin. ...

journal_title：Applied and environmental microbiology

authors： Naik HS,Duncan CL

更新日期：1977-08-01 00:00:00

abstract：:A novel death-specific gene, ScDSP, was obtained from a death stage subtraction cDNA library of the diatom Skeletonema costatum. The full length of ScDSP cDNA was 921 bp in length, containing a 699-bp open reading frame encoding 232 amino acids and two stretches of 66 and 156 bp in the 5' and 3' untranslated regions, ...

journal_title：Applied and environmental microbiology

authors： Chung CC,Hwang SP,Chang J

更新日期：2005-12-01 00:00:00

abstract：:The possible origin of beef contamination and genetic diversity of Escherichia coli populations in beef cattle, on carcasses and ground beef, was examined by using random amplification of polymorphic DNA (RAPD) and PCR-restriction fragment length polymorphism (PCR-RFLP) analysis of the fliC gene. E. coli was recovered...

journal_title：Applied and environmental microbiology

authors： Aslam M,Nattress F,Greer G,Yost C,Gill C,McMullen L

更新日期：2003-05-01 00:00:00

abstract：:Phages lytic to Vibrio vulnificus were found in estuarine waters, sediments, plankton, crustacea, molluscan shellfish, and the intestines of finfish of the U.S. Gulf Coast, but no apparent relationship between densities of V. vulnificus and its phages was observed. Phage diversity and abundance in molluscan shellfish ...

journal_title：Applied and environmental microbiology

authors： DePaola A,McLeroy S,McManus G

更新日期：1997-06-01 00:00:00

abstract：:Q fever, caused by Coxiella burnetii, is a zoonosis with a worldwide distribution. A large rural area in the southeast of the Netherlands was heavily affected by Q fever between 2007 and 2009. This initiated the development of a robust and internally controlled multiplex quantitative PCR (qPCR) assay for the detection...

journal_title：Applied and environmental microbiology

authors： de Bruin A,de Groot A,de Heer L,Bok J,Wielinga PR,Hamans M,van Rotterdam BJ,Janse I

更新日期：2011-09-01 00:00:00

abstract：:A new strategy for the detection of infectious Cryptosporidium parvum oocysts in water samples, which combines immunomagnetic separation (IMS) for recovery of oocysts with in vitro cell culturing and PCR (CC-PCR), was field tested with a total of 122 raw source water samples and 121 filter backwash water grab samples ...

journal_title：Applied and environmental microbiology

authors： Di Giovanni GD,Hashemi FH,Shaw NJ,Abrams FA,LeChevallier MW,Abbaszadegan M

更新日期：1999-08-01 00:00:00