Detection of Coxiella burnetii in complex matrices by using multiplex quantitative PCR during a major Q fever outbreak in The Netherlands.

abstract：:The potential for removing uranium from contaminated groundwater by stimulating the in situ activity of dissimilatory metal-reducing microorganisms was evaluated in a uranium-contaminated aquifer located in Rifle, Colo. Acetate (1 to 3 mM) was injected into the subsurface over a 3-month period via an injection gallery...

journal_title：Applied and environmental microbiology

authors： Anderson RT,Vrionis HA,Ortiz-Bernad I,Resch CT,Long PE,Dayvault R,Karp K,Marutzky S,Metzler DR,Peacock A,White DC,Lowe M,Lovley DR

更新日期：2003-10-01 00:00:00

abstract：:Opaque and translucent morphotypes of a TnphoA-containing strain of Vibrio vulnificus were fed to oysters, which were subsequently stored at temperatures ranging from 0.5 to 22 degrees C for 10 days. Samples of oysters were homogenized and plated at intervals to determine the cell density of V. vulnificus and total ae...

journal_title：Applied and environmental microbiology

authors： Murphy SK,Oliver JD

更新日期：1992-09-01 00:00:00

abstract：:An acclimated shake flask CO(2) evolution test was used to study the biodegradability of 14 commercial phthalate esters that are commonly used as plasticizers. Both CO(2) evolution (ultimate biodegradation) and loss of parent phthalate esters (primary biodegradation) were measured. With only a few exceptions, primary ...

journal_title：Applied and environmental microbiology

authors： Sugatt RH,O'grady DP,Banerjee S,Howard PH,Gledhill WE

更新日期：1984-04-01 00:00:00

abstract：:Growth of the Pseudomonas mendocina ymp strain on insoluble ferrihydrite is enhanced by exogenous reductants with concurrent increase in soluble iron concentrations. This shows that exogenous reductants play a substantial role in the overall microbial iron bioavailability. The exogenous reductants may work together wi...

journal_title：Applied and environmental microbiology

authors： Dhungana S,Anthony CR 3rd,Hersman LE

更新日期：2007-05-01 00:00:00

abstract：:It is now possible to obtain accurate total counts of the bacteria of natural waters with the use of acridine orange staining and epifluorescence microscopy. This approach can be coupled to highly sensitive measurements of heterotrophic activity using radioisotopes. To accomplish this, three variations of a "specific ...

journal_title：Applied and environmental microbiology

authors： Wright RT

更新日期：1978-08-01 00:00:00

abstract：:Analysis of electron spin resonance spectra of 5-doxyl stearic acid in aqueous suspensions of Clostridium acetobutylicum ATCC 824 and the butanol-tolerant SA-2 derivative during a small-scale fermentation at three different butanol challenge levels indicated that the SA-2 strain is able to respond to the physical flui...

journal_title：Applied and environmental microbiology

authors： Baer SH,Bryant DL,Blaschek HP

更新日期：1989-10-01 00:00:00

abstract：:Mechanisms for Fe(III) oxide reduction were investigated in Geothrix fermentans, a dissimilatory Fe(III)-reducing microorganism found within the Fe(III) reduction zone of subsurface environments. Culture filtrates of G. fermentans stimulated the reduction of poorly crystalline Fe(III) oxide by washed cell suspensions,...

journal_title：Applied and environmental microbiology

authors： Nevin KP,Lovley DR

更新日期：2002-05-01 00:00:00

abstract：:The N-chloramine compound 3-chloro-4,4-dimethyl-2-oxazolidinone (agent I) has been compared with calcium hypochlorite as to its efficacy as a bactericide for the treatment of water. The study included concentration, contact time, pH, temperature, and water quality as controlled variables. The species of bacteria teste...

journal_title：Applied and environmental microbiology

authors： Williams DE,Worley SD,Wheatley WB,Swango LJ

更新日期：1985-03-01 00:00:00

abstract：:The pyrene-degrading Mycobacterium sp. strain AP1 grew in nutrient-supplemented artificial seawater with a heavy fuel oil as the sole carbon source, causing the complete removal of all linear (C(12) to C(40)) and branched alkanes from the aliphatic fraction, as well as an extensive degradation of the three- and four-r...

journal_title：Applied and environmental microbiology

authors： Vila J,Grifoll M

更新日期：2009-10-01 00:00:00

abstract：:We studied injury of Escherichia coli O157:H7 cells in 11 food items during freeze storage and methods of isolating freeze-injured E. coli O157:H7 cells from foods. Food samples inoculated with E. coli O157:H7 were stored for 16 weeks at -20 degrees C in a freezer. Noninjured and injured cells were counted by using tr...

journal_title：Applied and environmental microbiology

authors： Hara-Kudo Y,Ikedo M,Kodaka H,Nakagawa H,Goto K,Masuda T,Konuma H,Kojima T,Kumagai S

更新日期：2000-07-01 00:00:00

abstract：:The influence of modified-atmosphere packaging, storage temperature, and time on survival and growth of Escherichia coli O157:H7 inoculated onto shredded lettuce, sliced cucumber, and shredded carrot was determined. Growth of psychotrophic and mesophilic microorganisms and changes in pH and sensory qualities of vegeta...

journal_title：Applied and environmental microbiology

authors： Abdul-Raouf UM,Beuchat LR,Ammar MS

更新日期：1993-07-01 00:00:00

abstract：:Ammonia monooxygenase of Nitrosomonas europaea catalyzes the oxidation of alkanes (up to C(8)) to alcohols and alkenes (up to C(5)) to epoxides and alcohols in the presence of ammonium ions. Straight-chain, N-terminal alkynes (up to C(10)) all exhibited a time-dependent inhibition of ammonia oxidation without effects ...

journal_title：Applied and environmental microbiology

authors： Hyman MR,Murton IB,Arp DJ

更新日期：1988-12-01 00:00:00

abstract：:Efficacious systems are described for the large-scale growth in tissue culture and concentration of infectious (P3HR-1) and transforming (B95-8) Epstein-Barr virus. Also recorded here are our updated procedures for growing stock cultures and protocols to harvest fluids containing biologically active virus which is inf...

journal_title：Applied and environmental microbiology

authors： Shibley GP,Manousos M,Munch K,Zelljadt I,Fisher L,Mayyasi S,Harewood K,Stevens R,Jensen KE

更新日期：1980-12-01 00:00:00

abstract：:Bacterial Halanaerobium strains become the dominant persisting microbial community member in produced fluids across geographically distinct hydraulically fractured shales. Halanaerobium is believed to be inadvertently introduced into this environment during the drilling and fracturing process and must therefore tolera...

journal_title：Applied and environmental microbiology

authors： Booker AE,Hoyt DW,Meulia T,Eder E,Nicora CD,Purvine SO,Daly RA,Moore JD,Wunch K,Pfiffner SM,Lipton MS,Mouser PJ,Wrighton KC,Wilkins MJ

更新日期：2019-05-30 00:00:00

abstract：:The 3-hydroxy fatty acids (3-OHFAs) in lipopolysaccharides (LPS) play an important role in determining endotoxin activity, and childhood exposure to endotoxin has recently been associated with reduced risk of atopic diseases. To characterize the 3-OHFAs in house dust (HD), we used gas chromatography-mass spectrometry ...

journal_title：Applied and environmental microbiology

authors： Park JH,Szponar B,Larsson L,Gold DR,Milton DK

更新日期：2004-01-01 00:00:00

abstract：:Decomposition of natural populations of Lake Mendota phytoplankton dominated by blue-green algae (cyanobacteria) was monitored by using oxygen uptake and disappearance of chlorophyll, algal volume (fluorescence microscopy), particulate protein, particulate organic carbon, and photosynthetic ability (CO(2) up-take). In...

journal_title：Applied and environmental microbiology

authors： Fallon RD,Brock TD

更新日期：1979-05-01 00:00:00

abstract：:The reliable detection of airborne biological threat agents depends on several factors, including the performance criteria of the detector and its operational environment. One step in improving the detector's performance is to increase our knowledge of the biological aerosol background in potential operational environ...

journal_title：Applied and environmental microbiology

authors： Dybwad M,Granum PE,Bruheim P,Blatny JM

更新日期：2012-03-01 00:00:00

abstract：:Population sizes of algae, bacteria, heterotrophic flagellates, and viruses were observed through the 1989 spring diatom bloom in Raunefjorden in western Norway. The culmination of the diatom bloom was followed by a peak in the concentration of bacteria and an increase in the concentration of heterotrophic flagellates...

journal_title：Applied and environmental microbiology

authors： Bratbak G,Heldal M,Norland S,Thingstad TF

更新日期：1990-05-01 00:00:00

abstract：:Isogenic strains of Escherichia coli O157:H7, missing either stx(2) or the entire Stx2-encoding phage, were compared with the parent strain for their abilities to colonize sheep. The absence of the phage or of the Shiga toxin did not significantly impact the magnitude or duration of shedding of E. coli O157:H7. ...

journal_title：Applied and environmental microbiology

authors： Cornick NA,Helgerson AF,Sharma V

更新日期：2007-01-01 00:00:00

abstract：:Microarray technology provides the opportunity to identify thousands of microbial genes or populations simultaneously, but low microbial biomass often prevents application of this technology to many natural microbial communities. We developed a whole-community genome amplification-assisted microarray detection approac...

journal_title：Applied and environmental microbiology

authors： Wu L,Liu X,Schadt CW,Zhou J

更新日期：2006-07-01 00:00:00

abstract：:Over 1,400 water samples were collected biweekly over 6 years from an intermittent stream protected and unprotected from pasturing cattle. The samples were monitored for host-specific Bacteroidales markers, Cryptosporidium species/genotypes, viruses and coliphages associated with humans or animals, and bacterial zoono...

journal_title：Applied and environmental microbiology

authors： Wilkes G,Brassard J,Edge TA,Gannon V,Jokinen CC,Jones TH,Marti R,Neumann NF,Ruecker NJ,Sunohara M,Topp E,Lapen DR

更新日期：2013-10-01 00:00:00

abstract：:In order to elucidate interactions between sulfate reduction and dechlorination, we systematically evaluated the effects of different concentrations of sulfate and sulfide on reductive dechlorination by isolates, constructed consortia, and enrichments containing Dehalococcoides sp. Sulfate (up to 5 mM) did not inhibit...

journal_title：Applied and environmental microbiology

authors： Mao X,Polasko A,Alvarez-Cohen L

更新日期：2017-03-31 00:00:00

abstract：:Gene clusters for biosynthesis of the fungal polyketides hypothemycin and radicicol from Hypomyces subiculosus and Pochonia chlamydosporia, respectively, were sequenced. Both clusters encode a reducing polyketide synthase (PKS) and a nonreducing PKS like those in the zearalenone cluster of Gibberella zeae, plus enzyme...

journal_title：Applied and environmental microbiology

authors： Reeves CD,Hu Z,Reid R,Kealey JT

更新日期：2008-08-01 00:00:00

abstract：:A trans unsaturated fatty acid was found as a major constituent in the lipids of Pseudomonas putida P8. The fatty acid was identified as 9-trans-hexadecenoic acid by gas chromatography, argentation thin-layer chromatography, and infrared absorption spectrometry. Growing cells of P. putida P8 reacted to the presence of...

journal_title：Applied and environmental microbiology

authors： Heipieper HJ,Diefenbach R,Keweloh H

更新日期：1992-06-01 00:00:00

abstract：:A nonsporulating strain of Streptomyces diastaticus producing alpha-L-arabinofuranosidase activity (EC 3.2-1.55) was isolated from soil. Two alpha-L-arabinosidases were purified by ion-exchange chromatography and chromatofocusing. The enzymes had molecular weights of 38,000 (C1) and 60,000 (C2) and pIs of 8.8 and 8.3,...

journal_title：Applied and environmental microbiology

authors： Tajana E,Fiechter A,Zimmermann W

更新日期：1992-05-01 00:00:00

abstract：:The phylogenetic diversity and species richness of ammonia-oxidizing archaea (AOA) and bacteria (AOB) were examined with aquarium biofiltration systems. Species richness, deduced from rarefaction analysis, and diversity indices indicated that the phylogenetic diversity and species richness of AOA are greater than thos...

journal_title：Applied and environmental microbiology

authors： Urakawa H,Tajima Y,Numata Y,Tsuneda S

更新日期：2008-02-01 00:00:00

abstract：:In this study, the feasibility of fluorescence lifetime imaging (FLIM) for measurement of RNA:DNA ratios in microorganisms was assessed. The fluorescence lifetime of a nucleic acid-specific probe (SYTO 13) was used to directly measure the RNA:DNA ratio inside living bacterial cells. In vitro, SYTO 13 showed shorter fl...

journal_title：Applied and environmental microbiology

authors： Walczysko P,Kuhlicke U,Knappe S,Cordes C,Neu TR

更新日期：2008-01-01 00:00:00

abstract：:In the biogeography of microorganisms, the habitat size of an attached-living bacterium has never been investigated. We approached this theme with a multilocus sequence analysis (MLSA) study of new strains of Rhodopirellula sp., an attached-living planctomycete. The development of an MLSA for Rhodopirellula baltica en...

journal_title：Applied and environmental microbiology

authors： Winkelmann N,Jaekel U,Meyer C,Serrano W,Rachel R,Rosselló-Mora R,Harder J

更新日期：2010-02-01 00:00:00

abstract：:This study provides a set of nutritional and environmental parameters suitable for the growth of morel (Morchella crassipes) sclerotia in the laboratory, using a modification of the jar method of Ower et al. (U.S. patent 4,594,809, June 1986). The optimum nutritional and environmental conditions for morel sclerotium f...

journal_title：Applied and environmental microbiology

authors： Volk TJ,Leonard TJ

更新日期：1989-12-01 00:00:00

abstract：:The effects of pressure and temperature on the fatty acid composition in a barotolerant deep-sea bacterium that had branched-chain fatty acids were examined. The major fatty acids of the strain at atmospheric pressure were iso-C(15:0), C(16:1), iso-C(17:0), and iso-C(17:1). As the growth pressure increased, the propor...

journal_title：Applied and environmental microbiology

authors： Kamimura K,Fuse H,Takimura O,Yamaoka Y

更新日期：1993-03-01 00:00:00