Abstract:
:In the biogeography of microorganisms, the habitat size of an attached-living bacterium has never been investigated. We approached this theme with a multilocus sequence analysis (MLSA) study of new strains of Rhodopirellula sp., an attached-living planctomycete. The development of an MLSA for Rhodopirellula baltica enabled the characterization of the genetic diversity at the species level, beyond the resolution of the 16S rRNA gene. The alleles of the nine housekeeping genes acsA, guaA, trpE, purH, glpF, fumC, icd, glyA, and mdh indicated the presence of 13 genetically defined operational taxonomic units (OTUs) in our culture collection. The MLSA-based OTUs coincided with the taxonomic units defined by DNA-DNA hybridization experiments. BOX-PCR supported the MLSA-based differentiation of two closely related OTUs. This study established a taxon-area relationship of cultivable Rhodopirellula species. In European seas, three closely related species covered the Baltic Sea and the eastern North Sea, the North Atlantic region, and the southern North Sea to the Mediterranean. The last had regional genotypes, as revealed by BOX-PCR. This suggests a limited habitat size of attached-living Rhodopirellula species.
journal_name
Appl Environ Microbioljournal_title
Applied and environmental microbiologyauthors
Winkelmann N,Jaekel U,Meyer C,Serrano W,Rachel R,Rosselló-Mora R,Harder Jdoi
10.1128/AEM.01525-09subject
Has Abstractpub_date
2010-02-01 00:00:00pages
776-85issue
3eissn
0099-2240issn
1098-5336pii
AEM.01525-09journal_volume
76pub_type
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journal_title:Applied and environmental microbiology
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journal_title:Applied and environmental microbiology
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更新日期:1987-09-01 00:00:00
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journal_title:Applied and environmental microbiology
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