Pyrosequencing-based assessment of soil pH as a predictor of soil bacterial community structure at the continental scale.

Abstract:

:Soils harbor enormously diverse bacterial populations, and soil bacterial communities can vary greatly in composition across space. However, our understanding of the specific changes in soil bacterial community structure that occur across larger spatial scales is limited because most previous work has focused on either surveying a relatively small number of soils in detail or analyzing a larger number of soils with techniques that provide little detail about the phylogenetic structure of the bacterial communities. Here we used a bar-coded pyrosequencing technique to characterize bacterial communities in 88 soils from across North and South America, obtaining an average of 1,501 sequences per soil. We found that overall bacterial community composition, as measured by pairwise UniFrac distances, was significantly correlated with differences in soil pH (r = 0.79), largely driven by changes in the relative abundances of Acidobacteria, Actinobacteria, and Bacteroidetes across the range of soil pHs. In addition, soil pH explains a significant portion of the variability associated with observed changes in the phylogenetic structure within each dominant lineage. The overall phylogenetic diversity of the bacterial communities was also correlated with soil pH (R(2) = 0.50), with peak diversity in soils with near-neutral pHs. Together, these results suggest that the structure of soil bacterial communities is predictable, to some degree, across larger spatial scales, and the effect of soil pH on bacterial community composition is evident at even relatively coarse levels of taxonomic resolution.

journal_name

Appl Environ Microbiol

authors

Lauber CL,Hamady M,Knight R,Fierer N

doi

10.1128/AEM.00335-09

subject

Has Abstract

pub_date

2009-08-01 00:00:00

pages

5111-20

issue

15

eissn

0099-2240

issn

1098-5336

pii

AEM.00335-09

journal_volume

75

pub_type

杂志文章
  • Evaluation of the Biolog automated microbial identification system.

    abstract::Biolog's identification system was used to identify 39 American Type Culture Collection reference taxa and 45 gram-negative isolates from water samples. Of the reference strains, 98% were identified to genus level and 76% to species level within 4 to 24 h. Identification of some authentic strains of Enterobacter, Kl...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.58.6.2089-2092.1992

    authors: Klingler JM,Stowe RP,Obenhuber DC,Groves TO,Mishra SK,Pierson DL

    更新日期:1992-06-01 00:00:00

  • Gray mold populations in german strawberry fields are resistant to multiple fungicides and dominated by a novel clade closely related to Botrytis cinerea.

    abstract::The gray mold fungus Botrytis cinerea is a major threat to fruit and vegetable production. Strawberry fields usually receive several fungicide treatments against Botrytis per season. Gray mold isolates from several German strawberry-growing regions were analyzed to determine their sensitivity against botryticides. Fun...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.02655-12

    authors: Leroch M,Plesken C,Weber RW,Kauff F,Scalliet G,Hahn M

    更新日期:2013-01-01 00:00:00

  • Geographical segregation of the neurotoxin-producing cyanobacterium Anabaena circinalis.

    abstract::Blooms of the cyanobacterium Anabaena circinalis are a major worldwide problem due to their production of a range of toxins, in particular the neurotoxins anatoxin-a and paralytic shellfish poisons (PSPs). Although there is a worldwide distribution of A. circinalis, there is a geographical segregation of neurotoxin pr...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/aem.66.10.4468-4474.2000

    authors: Beltran EC,Neilan BA

    更新日期:2000-10-01 00:00:00

  • Lactobacillus bulgaricus proteinase expressed in Lactococcus lactis is a powerful carrier for cell wall-associated and secreted bovine beta-lactoglobulin fusion proteins.

    abstract::Lactic acid bacteria have a good potential as agents for the delivery of heterologous proteins to the gastrointestinal mucosa and thus for the reequilibration of inappropriate immune responses to food antigens. Bovine beta-lactoglobulin (BLG) is considered a major allergen in cow's milk allergy. We have designed recom...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/aem.68.6.2917-2923.2002

    authors: Bernasconi E,Germond JE,Delley M,Fritsché R,Corthésy B

    更新日期:2002-06-01 00:00:00

  • Isolation of Azospirillum lipoferum 4T Tn5 Mutants Affected in Melanization and Laccase Activity.

    abstract::Azospirillum lipoferum 4T has original properties such as nonmotility, melanin synthesis, and laccase activity. Following random Tn5 mutagenesis in A. lipoferum 4T, we obtained 10 mutants which were affected in melanization and laccase activity. The class 1 mutants, with intermediate levels of laccase activity, showed...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.60.9.3413-3415.1994

    authors: Faure D,Bouillant ML,Bally R

    更新日期:1994-09-01 00:00:00

  • Comparison of methods for DNA isolation from food samples for detection of Shiga toxin-producing Escherichia coli by real-time PCR.

    abstract::In this study, food samples were intentionally contaminated with Escherichia coli O157:H7, and then DNA was isolated by using four commercial kits. The isolated DNA samples were compared by using real-time PCR detection of the Shiga toxin genes. The four kits tested worked similarly. ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/aem.69.3.1844-1846.2003

    authors: Heller LC,Davis CR,Peak KK,Wingfield D,Cannons AC,Amuso PT,Cattani J

    更新日期:2003-03-01 00:00:00

  • Form of Vitamin B(12) and Its Role in a Methanol-Utilizing Bacterium Protaminobacter ruber.

    abstract::A methanol-utilizing bacterium, Protaminobacter ruber, produced a large amount of vitamin B(12). The compounds were isolated from the cells and identified as methylcobalamin (methyl-B(12)) and adenosylcobalamin (adenosyl-B(12)) by various tests. The variation in the form of B(12) during cultivation was examined by bio...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.33.3.515-521.1977

    authors: Sato K,Ueda S,Shimizu S

    更新日期:1977-03-01 00:00:00

  • Characterization of cry genes in a Mexican Bacillus thuringiensis strain collection.

    abstract::Mexico is located in a transition zone between the Nearctic and Neotropical biogeographical regions and contains a rich and unique biodiversity. A total of 496 Bacillus thuringiensis strains were isolated from 503 soil samples collected from the five macroregions of the country. The characterization of the strain coll...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.64.12.4965-4972.1998

    authors: Bravo A,Sarabia S,Lopez L,Ontiveros H,Abarca C,Ortiz A,Ortiz M,Lina L,Villalobos FJ,Peña G,Nuñez-Valdez ME,Soberón M,Quintero R

    更新日期:1998-12-01 00:00:00

  • Energetics of arginine and lysine transport by whole cells and membrane vesicles of strain SR, a monensin-sensitive ruminal bacterium.

    abstract::Strain SR, a monensin-sensitive, ammonia-producing ruminal bacterium, grew rapidly on arginine and lysine, but only if sodium was present. Arginine transport could be driven by either an electrical potential or a chemical gradient of sodium. Arginine was converted to ornithine, and it appeared that ornithine efflux cr...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.58.3.969-975.1992

    authors: Van Kessel JS,Russell JB

    更新日期:1992-03-01 00:00:00

  • Biochemical analyses of multiple endoxylanases from the rumen bacterium Ruminococcus albus 8 and their synergistic activities with accessory hemicellulose-degrading enzymes.

    abstract::Ruminococcus albus 8 is a ruminal bacterium capable of metabolizing hemicellulose and cellulose, the major components of the plant cell wall. The enzymes that allow this bacterium to capture energy from the two polysaccharides, therefore, have potential application in plant cell wall depolymerization, a process critic...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.00353-11

    authors: Moon YH,Iakiviak M,Bauer S,Mackie RI,Cann IK

    更新日期:2011-08-01 00:00:00

  • Expression of the arginine deiminase pathway genes in Lactobacillus sakei is strain dependent and is affected by the environmental pH.

    abstract::The adaptation of Lactobacillus sakei to a meat environment is reflected in its metabolic potential. For instance, the ability to utilize arginine through the arginine deiminase (ADI) pathway, resulting in additional ATP, represents a competitive benefit. In L. sakei CTC 494, the arc operon (arcABCTDR) shows the same ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.07724-11

    authors: Rimaux T,Rivière A,Illeghems K,Weckx S,De Vuyst L,Leroy F

    更新日期:2012-07-01 00:00:00

  • Cell wall polysaccharide synthases are located in detergent-resistant membrane microdomains in oomycetes.

    abstract::The pathways responsible for cell wall polysaccharide biosynthesis are vital in eukaryotic microorganisms. The corresponding synthases are potential targets of inhibitors such as fungicides. Despite their fundamental and economical importance, most polysaccharide synthases are not well characterized, and their molecul...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.02728-08

    authors: Briolay A,Bouzenzana J,Guichardant M,Deshayes C,Sindt N,Bessueille L,Bulone V

    更新日期:2009-04-01 00:00:00

  • Use of acid treatment and a selective medium to enhance the recovery of Francisella tularensis from water.

    abstract::Francisella tularensis has been associated with naturally occurring waterborne outbreaks and is also of interest as a potential biological weapon. Recovery of this pathogen from water using cultural methods is challenging due to the organism's fastidious growth requirements and interference by indigenous bacteria. A 1...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.05226-11

    authors: Humrighouse BW,Adcock NJ,Rice EW

    更新日期:2011-09-01 00:00:00

  • Integration of metabolic modeling and phenotypic data in evaluation and improvement of ethanol production using respiration-deficient mutants of Saccharomyces cerevisiae.

    abstract::Flux balance analysis and phenotypic data were used to provide clues to the relationships between the activities of gene products and the phenotypes resulting from the deletion of genes involved in respiratory function in Saccharomyces cerevisiae. The effect of partial or complete respiratory deficiency on the ethanol...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.00009-08

    authors: Dikicioglu D,Pir P,Onsan ZI,Ulgen KO,Kirdar B,Oliver SG

    更新日期:2008-09-01 00:00:00

  • Genetic characterization of plasmid-associated benzalkonium chloride resistance determinants in a Listeria monocytogenes strain from the 1998-1999 outbreak.

    abstract::Quaternary ammonium compounds such as benzalkonium chloride (BC) are widely used as disinfectants in both food processing and medical environments. BC-resistant strains of Listeria monocytogenes have been implicated in multistate outbreaks of listeriosis and have been frequently isolated from food processing plants. H...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.02056-10

    authors: Elhanafi D,Dutta V,Kathariou S

    更新日期:2010-12-01 00:00:00

  • Pgas, a Low-pH-Induced Promoter, as a Tool for Dynamic Control of Gene Expression for Metabolic Engineering of Aspergillus niger.

    abstract::The dynamic control of gene expression is important for adjusting fluxes in order to obtain desired products and achieve appropriate cell growth, particularly when the synthesis of a desired product drains metabolites required for cell growth. For dynamic gene expression, a promoter responsive to a particular environm...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.03222-16

    authors: Yin X,Shin HD,Li J,Du G,Liu L,Chen J

    更新日期:2017-03-02 00:00:00

  • Occurrence of fusaproliferin and beauvericin in Fusarium-contaminated livestock feed in Iowa.

    abstract::Fusarium fungal contaminants and related mycotoxins were investigated in eight maize feed samples submitted to the Iowa State University Veterinary Diagnostic Laboratory. Fusarium moniliforme, F. proliferatum, and F. subglutinans were isolated from seven, eight, and five samples, respectively. These strains belonged t...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.64.10.3923-3926.1998

    authors: Munkvold G,Stahr HM,Logrieco A,Moretti A,Ritieni A

    更新日期:1998-10-01 00:00:00

  • Metabolic Capability and Phylogenetic Diversity of Mono Lake during a Bloom of the Eukaryotic Phototroph Picocystis sp. Strain ML.

    abstract::Algal blooms in lakes are often associated with anthropogenic eutrophication; however, they can occur without the human introduction of nutrients to a lake. A rare bloom of the alga Picocystis sp. strain ML occurred in the spring of 2016 at Mono Lake, a hyperalkaline lake in California, which was also at the apex of a...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.01171-18

    authors: Stamps BW,Nunn HS,Petryshyn VA,Oremland RS,Miller LG,Rosen MR,Bauer KW,Thompson KJ,Tookmanian EM,Waldeck AR,Loyd SJ,Johnson HA,Stevenson BS,Berelson WM,Corsetti FA,Spear JR

    更新日期:2018-10-17 00:00:00

  • Comparative transcriptomic and phenotypic analysis of the responses of Bacillus cereus to various disinfectant treatments.

    abstract::Antimicrobial chemicals are widely applied to clean and disinfect food-contacting surfaces. However, the cellular response of bacteria to various disinfectants is unclear. In this study, the physiological and genome-wide transcriptional responses of Bacillus cereus ATCC 14579 exposed to four different disinfectants (b...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.03003-09

    authors: Ceragioli M,Mols M,Moezelaar R,Ghelardi E,Senesi S,Abee T

    更新日期:2010-05-01 00:00:00

  • Deep-Subsurface Pressure Stimulates Metabolic Plasticity in Shale-Colonizing Halanaerobium spp.

    abstract::Bacterial Halanaerobium strains become the dominant persisting microbial community member in produced fluids across geographically distinct hydraulically fractured shales. Halanaerobium is believed to be inadvertently introduced into this environment during the drilling and fracturing process and must therefore tolera...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.00018-19

    authors: Booker AE,Hoyt DW,Meulia T,Eder E,Nicora CD,Purvine SO,Daly RA,Moore JD,Wunch K,Pfiffner SM,Lipton MS,Mouser PJ,Wrighton KC,Wilkins MJ

    更新日期:2019-05-30 00:00:00

  • Effect of chlorine dioxide gas on fungi and mycotoxins associated with sick building syndrome.

    abstract::The growth of indoor molds and their resulting products (e.g., spores and mycotoxins) can present health hazards for human beings. The efficacy of chlorine dioxide gas as a fumigation treatment for inactivating sick building syndrome-related fungi and their mycotoxins was evaluated. Filter papers (15 per organism) fea...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.71.9.5399-5403.2005

    authors: Wilson SC,Wu C,Andriychuk LA,Martin JM,Brasel TL,Jumper CA,Straus DC

    更新日期:2005-09-01 00:00:00

  • Development and application of a new method to extract bacterial DNA from soil based on separation of bacteria from soil with cation-exchange resin.

    abstract::A new method for the extraction of bacterial DNA from soil has been developed. Soil samples of 50 g were dispersed, and bacteria were released by use of a cation-exchange resin; subsequently, bacteria were separated from soil particles by low-speed centrifugation and lysed with lysozyme and ionic detergent, and the DN...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.58.8.2458-2462.1992

    authors: Jacobsen CS,Rasmussen OF

    更新日期:1992-08-01 00:00:00

  • Synthesis of [C]Cellobiose with Clostridium thermocellum Cellobiose Phosphorylase.

    abstract::Cellobiose labeled either at the reducing end (4-O-beta-d-glucopyranosyl-d-[U-C]glucopyranose) or at the nonreducing end (4-O-beta-d-[U-C]glucopyranosyl-d -glucopyranose) was synthesized with Clostridium thermocellum cellobiose phosphorylase at greater than 98% purity. The radioactive cellobioses were identical to aut...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.52.4.902-904.1986

    authors: Ng TK,Zeikus JG

    更新日期:1986-10-01 00:00:00

  • Sensitive and specific detection of Listeria monocytogenes in milk and ground beef with the polymerase chain reaction.

    abstract::A sensitive and specific method for detection of Listeria monocytogenes in milk and ground-beef samples is described. It consists of culturing samples in listeria enrichment broth (LEB) and subculturing them from LEB to listeria plating media, followed by DNA extraction and species-specific detection of the organism b...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.57.9.2576-2580.1991

    authors: Thomas EJ,King RK,Burchak J,Gannon VP

    更新日期:1991-09-01 00:00:00

  • Thermal inactivation and injury of Bacillus stearothermophilus spores.

    abstract::Aqueous spore suspensions of Bacillus stearothermophilus ATCC 12980 were heated at different temperatures for various time intervals in a resistometer, spread plated on antibiotic assay medium supplemented with 0.1% soluble starch without (AAMS) or with (AAMS-S) 0.9% NaCl, and incubated at 55 degrees C unless otherwis...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.53.2.365-370.1987

    authors: Feeherry FE,Munsey DT,Rowley DB

    更新日期:1987-02-01 00:00:00

  • Minireplicon from pBtoxis of Bacillus thuringiensis subsp. israelensis.

    abstract::A 2.2-kb fragment containing a replicon from pBtoxis, the large plasmid that encodes the insecticidal endotoxins of Bacillus thuringiensis subsp. israelensis, was identified, cloned, and sequenced. This fragment contains cis elements, including iterons, found in replication origins of other large plasmids and suggests...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.00976-06

    authors: Tang M,Bideshi DK,Park HW,Federici BA

    更新日期:2006-11-01 00:00:00

  • Multiplex reverse transcription-PCR for simultaneous detection of beet necrotic yellow vein virus, Beet soilborne virus, and Beet virus Q and their vector Polymyxa betae KESKIN on sugar beet.

    abstract::Three soilborne viruses transmitted by Polymyxa betae KESKIN in sugar beet have been described: Beet necrotic yellow vein virus (BNYVV), the agent of rhizomania, Beet soilborne virus (BSBV), and Beet virus Q (BVQ). A multiplex reverse transcription-PCR technique was developed to simultaneously detect BNYVV, BSBV, and ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/aem.69.4.2356-2360.2003

    authors: Meunier A,Schmit JF,Stas A,Kutluk N,Bragard C

    更新日期:2003-04-01 00:00:00

  • Posttranslational Targeting of a Recombinant Protein Promotes Its Efficient Secretion into the Escherichia coli Periplasm.

    abstract::Many recombinant proteins that are produced in Escherichia coli have to be targeted to the periplasm to be functional. N-terminal signal peptides can be used to direct recombinant proteins to the membrane-embedded Sec translocon, a multiprotein complex that translocates proteins across the membrane into the periplasm....

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.00671-19

    authors: Ytterberg AJ,Zubarev RA,Baumgarten T

    更新日期:2019-06-17 00:00:00

  • Cloning and nucleotide sequences of the homoserine dehydrogenase genes (hom) and the threonine synthase genes (thrC) of the gram-negative obligate methylotroph Methylobacillus glycogenes.

    abstract::We have cloned the homoserine dehydrogenase genes (hom) from the gram-negative obligate methylotrophs Methylobacillus glycogenes ATCC 21276 and ATCC 21371 by complementation of an Escherichia coli homoserine dehydrogenase-deficient mutant. The 4.15-kb DNA fragment cloned from M. glycogenes ATCC 21371 also complemented...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.60.1.111-119.1994

    authors: Motoyama H,Maki K,Anazawa H,Ishino S,Teshiba S

    更新日期:1994-01-01 00:00:00

  • Enzymatic manganese(II) oxidation by a marine alpha-proteobacterium.

    abstract::A yellow-pigmented marine bacterium, designated strain SD-21, was isolated from surface sediments of San Diego Bay, San Diego, Calif., based on its ability to oxidize soluble Mn(II) to insoluble Mn(III, IV) oxides. 16S rRNA analysis revealed that this organism was most closely related to members of the genus Erythroba...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/aem.67.9.4024-4029.2001

    authors: Francis CA,Co EM,Tebo BM

    更新日期:2001-09-01 00:00:00