Development and application of a new method to extract bacterial DNA from soil based on separation of bacteria from soil with cation-exchange resin.

abstract：:Sulfolobus solfataricus is an aerobic crenarchaeal hyperthermophile with optimum growth at temperatures greater than 80°C and pH 2 to 4. Within the crenarchaeal group of Sulfolobales, N-acetylglucosamine (GlcNAc) has been shown to be a component of exopolysaccharides, forming their biofilms, and of the N-glycan decora...

journal_title：Applied and environmental microbiology

authors： Iacono R,Strazzulli A,Maurelli L,Curci N,Casillo A,Corsaro MM,Moracci M,Cobucci-Ponzano B

更新日期：2019-01-09 00:00:00

abstract：:In studies designed to determine the factors responsible for control of herpesvirus replicaton in an infected cell, we examined the interaction of varicella-zoster (VZ) virus-infected human embryonic lung cells with the pesticide carbaryl. The replication of the cell-associated VZ virus was enhanced 2- to 13-fold as c...

journal_title：Applied and environmental microbiology

authors： Abrahamsen LH,Jerkofsky M

更新日期：1981-03-01 00:00:00

abstract：:The axy43A gene encoding the intracellular trifunctional xylanolytic enzyme from Paenibacillus curdlanolyticus B-6 was cloned and expressed in Escherichia coli Recombinant PcAxy43A consisting of a glycoside hydrolase family 43 and a family 6 carbohydrate-binding module exhibited endo-xylanase, β-xylosidase, and arabin...

journal_title：Applied and environmental microbiology

authors： Teeravivattanakit T,Baramee S,Phitsuwan P,Waeonukul R,Pason P,Tachaapaikoon C,Sakka K,Ratanakhanokchai K

更新日期：2016-12-01 00:00:00

abstract：:Mycobacterium vaccae can catabolize a number of major groundwater pollutants. When added singly, acetone, cyclohexane, styrene, benzene, ethylbenzene, propylbenzene, dioxane, and 1,2-dichloroethylene can be catabolized by M. vaccae. Catabolism of a number of these chemicals was monitored by gas-chromatographic analysi...

journal_title：Applied and environmental microbiology

authors： Burback BL,Perry JJ

更新日期：1993-04-01 00:00:00

abstract：:Small streams and their headwaters are key sources of microbial diversity in fluvial systems and serve as an entry point for bacteria from surrounding environments. Community assembly processes occurring in these streams shape downstream population structure and nutrient cycles. To elucidate the development and stabil...

journal_title：Applied and environmental microbiology

authors： Teachey ME,McDonald JM,Ottesen EA

更新日期：2019-05-16 00:00:00

abstract：:The chlorosis-inducing phytotoxin coronatine is produced by several Pseudomonas syringae pathovars, including glycinea, morsprunorum, atropurpurea, and the closely related tomato and maculicola. To date, all coronatine-producing pv. glycinea, morsprunorum, and atropurpurea strains that have been examined carry the gen...

journal_title：Applied and environmental microbiology

authors： Cuppels DA,Ainsworth T

更新日期：1995-10-01 00:00:00

abstract：:This study was aimed at assessing whether the repeated exposure of 12 strains of Salmonella spp., Escherichia coli, and Listeria monocytogenes to alternative nonthermal decontamination techniques with UV light (UV-C) and nonthermal atmospheric plasma (NTAP) may cause the emergence of variants showing increased resista...

journal_title：Applied and environmental microbiology

authors： Álvarez-Molina A,de Toro M,Ruiz L,López M,Prieto M,Alvarez-Ordóñez A

更新日期：2020-04-17 00:00:00

abstract：:Isolates of Gaeumannomyces graminis var. tritici, the causal agent of take-all of wheat, varied in sensitivity in vitro to the antibiotics phenazine-1-carboxylic acid (PCA) and 2,4-diacetylphloroglucinol (Phl) produced by fluorescent Pseudomonas spp. shown previously to have potential for biological control of this pa...

journal_title：Applied and environmental microbiology

authors： Mazzola M,Fujimoto DK,Thomashow LS,Cook RJ

更新日期：1995-07-01 00:00:00

abstract：:An amylosucrase gene was subjected to high-rate segmental random mutagenesis, which was directed toward a segment encoding amino acids that influence the interaction with substrate molecules in subsites -1 to +3. A screen was used to identify enzyme variants with compromised glucan chain elongation. With an average mu...

journal_title：Applied and environmental microbiology

authors： Schneider J,Fricke C,Overwin H,Hofmann B,Hofer B

更新日期：2009-12-01 00:00:00

abstract：:Vibrio vulnificus is a human and animal pathogen that carries the highest death rate of any food-borne disease agent. It colonizes shellfish and forms biofilms on the surfaces of plankton, algae, fish, and eels. Greater understanding of biofilm formation by the organism could provide insight into approaches to decreas...

journal_title：Applied and environmental microbiology

authors： Nakhamchik A,Wilde C,Rowe-Magnus DA

更新日期：2008-07-01 00:00:00

abstract：:This was the first molecular study of the bacterial flora of the sheep scab mite (Psoroptes ovis). A sequence analysis of genes coding for 16S rRNA revealed that Serratia marcescens and bacteria closely related to Staphylococcus intermedius or Staphylococcus chromogens and Alloiococcus otitidis were present. These bac...

journal_title：Applied and environmental microbiology

authors： Hogg JC,Lehane MJ

更新日期：1999-09-01 00:00:00

abstract：:Bacillus subtilis and Bacillus licheniformis are widely used for the large-scale industrial production of proteins. These strains can efficiently secrete proteins into the culture medium using the general secretion (Sec) pathway. A characteristic feature of all secreted proteins is their N-terminal signal peptides, wh...

journal_title：Applied and environmental microbiology

authors： Degering C,Eggert T,Puls M,Bongaerts J,Evers S,Maurer KH,Jaeger KE

更新日期：2010-10-01 00:00:00

abstract：:Rotavirus virus-like particles (VLPs) and MS2 bacteriophages were bioaccumulated in bivalve mollusks to evaluate viral persistence in shellfish during depuration and relaying under natural conditions. Using this nonpathogenic surrogate virus, we were able to demonstrate that about 1 log10 of VLPs was depurated after 1...

journal_title：Applied and environmental microbiology

authors： Loisy F,Atmar RL,Le Saux JC,Cohen J,Caprais MP,Pommepuy M,Le Guyader FS

更新日期：2005-10-01 00:00:00

abstract：:Thirty years of intensive research have significantly contributed to our understanding of Helicobacter pylori biology and pathogenesis. However, the lack of convenient genetic tools, in particular the limited effectiveness of available reporter systems, has notably limited the toolbox for fundamental and applied studi...

journal_title：Applied and environmental microbiology

authors： Vannini A,Agriesti F,Mosca F,Roncarati D,Scarlato V,Danielli A

更新日期：2012-09-01 00:00:00

abstract：:The detection and enumeration of most cultivable human enteric viruses from water is possible if samples are first inoculated onto a suitable cell line such as MA-104 or BGM. Virus growth is then detected by an indirect immunoperoxidase method with human immune serum globulin as the source of antibody to most enteric ...

journal_title：Applied and environmental microbiology

authors： Payment P,Trudel M

更新日期：1985-11-01 00:00:00

abstract：:Escherichia coli isolates from rectal swabs from 62 chickens and stools from 42 children living in close contact with chickens on the same farms in Kiambu district, Kenya, were compared for their genetic relatedness. Antibiotic susceptibility profiles broadly categorized isolates from the children and from the chicken...

journal_title：Applied and environmental microbiology

authors： Kariuki S,Gilks C,Kimari J,Obanda A,Muyodi J,Waiyaki P,Hart CA

更新日期：1999-02-01 00:00:00

abstract：:Surfaces made of copper or its alloys have strong antimicrobial properties against a wide variety of microorganisms. However, the molecular mode of action responsible for the antimicrobial efficacy of metallic copper is not known. Here, we show that dry copper surfaces inactivate Candida albicans and Saccharomyces cer...

journal_title：Applied and environmental microbiology

authors： Quaranta D,Krans T,Espírito Santo C,Elowsky CG,Domaille DW,Chang CJ,Grass G

更新日期：2011-01-01 00:00:00

abstract：:Enteric microbiota play a variety of roles in intestinal health and disease. While bacteria in the intestine have been broadly characterized, little is known about the abundance or diversity of enteric fungi. This study utilized a culture-independent method termed oligonucleotide fingerprinting of rRNA genes (OFRG) to...

journal_title：Applied and environmental microbiology

authors： Scupham AJ,Presley LL,Wei B,Bent E,Griffith N,McPherson M,Zhu F,Oluwadara O,Rao N,Braun J,Borneman J

更新日期：2006-01-01 00:00:00

abstract：:Microbial transformation of lapachol, a naturally occurring naphthoquinone, was carried out by Curvularia lunata (NRRL 2178). The fungus brings about oxidative cyclization of the substrate to dehydro-alpha-lapachone, which was isolated and characterized by nuclear magnetic resonance and mass spectral analyses; its str...

journal_title：Applied and environmental microbiology

authors： Otten S,Rosazza JP

更新日期：1979-08-01 00:00:00

abstract：:Upflow, anaerobic, fixed-bed, activated charcoal biotreatment columns capable of operating at free cyanide concentrations of greater than 100 mg liter-1 with a hydraulic retention time of less than 48 h were developed. Methanogenesis was maintained under a variety of feed medium conditions which included ethanol, phen...

journal_title：Applied and environmental microbiology

authors： Fallon RD,Cooper DA,Speece R,Henson M

更新日期：1991-06-01 00:00:00

abstract：:The occurrence of 10 pathogens and three fecal indicators was assessed by quantitative PCR in manures of Australian feedlot cattle. Most samples tested positive for one or more pathogens. For the dominant pathogens Campylobacter jejuni, Listeria monocytogenes, Giardia spp., Cryptosporidium spp., and eaeA-positive Esch...

journal_title：Applied and environmental microbiology

authors： Klein M,Brown L,Tucker RW,Ashbolt NJ,Stuetz RM,Roser DJ

更新日期：2010-10-01 00:00:00

abstract：:A bile salt hydrolase (BSH) was isolated from Bifidobacterium longum SBT2928, purified, and characterized. Furthermore, we describe for the first time cloning and analysis of the gene encoding BSH (bsh) in a member of the genus Bifidobacterium. The enzyme has a native molecular weight of 125,000 to 130,000 and a subun...

journal_title：Applied and environmental microbiology

authors： Tanaka H,Hashiba H,Kok J,Mierau I

更新日期：2000-06-01 00:00:00

abstract：:Since their initial discovery in samples from the north Atlantic Ocean, 16S rRNA genes related to the environmental gene clone cluster known as SAR202 have been recovered from pelagic freshwater, marine sediment, soil, and deep subsurface terrestrial environments. Together, these clones form a major, monophyletic subg...

journal_title：Applied and environmental microbiology

authors： Morris RM,Rappé MS,Urbach E,Connon SA,Giovannoni SJ

更新日期：2004-05-01 00:00:00

abstract：:Bacillus sp. strain SS, an aerobic, gram-positive sporeformer, was isolated from seeds of Astragalus crotalariae, a selenium-accumulating plant. This bacillus grew in a nutrient broth (containing beef extract and peptone) if the medium was supplemented with high concentrations of selenium. Concentrations of Na(2)SeO(3...

journal_title：Applied and environmental microbiology

authors： Lindblow-Kull C,Shrift A,Gherna RL

更新日期：1982-09-01 00:00:00

abstract：:A radioisotope method was devised to study bacterial respiratory reduction of arsenate in sediments. The following two arsenic-rich soda lakes in California were chosen for comparison on the basis of their different salinities: Mono Lake (approximately 90 g/liter) and Searles Lake (approximately 340 g/liter). Profiles...

journal_title：Applied and environmental microbiology

authors： Kulp TR,Hoeft SE,Miller LG,Saltikov C,Murphy JN,Han S,Lanoil B,Oremland RS

更新日期：2006-10-01 00:00:00

abstract：:Eukaryotic marine microbes play pivotal roles in biogeochemical nutrient cycling and ecosystem function, but studies that focus on the protistan biogeography and genetic diversity lag-behind studies of other microbes. 18S rRNA PCR amplification and clone library sequencing are commonly used to assess diversity that is...

journal_title：Applied and environmental microbiology

authors： Koid A,Nelson WC,Mraz A,Heidelberg KB

更新日期：2012-06-01 00:00:00

abstract：:Examination of cucumber roots (Cucumis sativus L.) grown in bark compost media and of the surrounding edaphic substrate showed profiles of polar lipid fatty acids commonly found in bacteria. The composition of fatty acids in these profiles differed significantly between roots grown in a medium naturally suppressive to...

journal_title：Applied and environmental microbiology

authors： Tunlid A,Hoitink HA,Low C,White DC

更新日期：1989-06-01 00:00:00

abstract：:This study reports on an emerging fungal disease of the edible crab, Cancer pagurus. Juvenile (prerecruit) crabs were found to be subject to this disease condition during the months of May to September at two intertidal sites in South Wales, United Kingdom. Histopathology revealed that the fungi overwhelm the host res...

journal_title：Applied and environmental microbiology

authors： Smith AL,Hamilton KM,Hirschle L,Wootton EC,Vogan CL,Pope EC,Eastwood DC,Rowley AF

更新日期：2013-02-01 00:00:00

abstract：:The structural gene coding for the lysine-sensitive aspartokinase II of the methylotrophic thermotolerant Bacillus sp. strain MGA3 was cloned from a genomic library by complementation of an Escherichia coli auxotrophic mutant lacking all three aspartokinase isozymes. The nucleotide sequence of the entire 2.2-kb PstI f...

journal_title：Applied and environmental microbiology

authors： Schendel FJ,Flickinger MC

更新日期：1992-09-01 00:00:00

abstract：:The thermophilic bacterium Bacillus thermoantarcticus produces two exocellular polysaccharides (EPS 1 and EPS 2), which can be obtained from the supernatant of liquid cultures by cold-ethanol precipitation, in yields as high as 400 mg liter(sup-1). The EPS fraction was produced with all substrates tested, although a h...

journal_title：Applied and environmental microbiology

authors： Manca MC,Lama L,Improta R,Esposito E,Gambacorta A,Nicolaus B

更新日期：1996-09-01 00:00:00