Abstract:
:The detection and enumeration of most cultivable human enteric viruses from water is possible if samples are first inoculated onto a suitable cell line such as MA-104 or BGM. Virus growth is then detected by an indirect immunoperoxidase method with human immune serum globulin as the source of antibody to most enteric viruses. The number of positive cell cultures in the immunoperoxidase assay is used to calculate the virus titer (as a most probable number) in the sample assayed.
journal_name
Appl Environ Microbioljournal_title
Applied and environmental microbiologyauthors
Payment P,Trudel Mdoi
10.1128/AEM.50.5.1308-1310.1985subject
Has Abstractpub_date
1985-11-01 00:00:00pages
1308-10issue
5eissn
0099-2240issn
1098-5336journal_volume
50pub_type
杂志文章abstract::Intracellular granules containing ferric and ferrous iron formed in Shewanella putrefaciens CN32 during dissimilatory reduction of solid-phase ferric iron. It is the first in situ detection at high resolution (150 nm) of a mixed-valence metal particle residing within a prokaryotic cell. The relationship of the interna...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.01492-06
更新日期:2007-02-01 00:00:00
abstract::An amylolytic Lactobacillus plantarum silage strain with the starch-degrading ability displayed by Lactobacillus amylovorus was developed. An active fragment of the gene coding for alpha-amylase production in L. amylovorus was cloned and integrated into the chromosome of the competitive inoculant strain L. plantarum L...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.60.10.3529-3535.1994
更新日期:1994-10-01 00:00:00
abstract::Of 44 fluorogenic substrates tested for their ability to differentiate species of fecal streptococci, four yielded species-differentiating reactions. The remaining substrates either yielded uniformly positive, negative, or variable strain-dependent reactions. One substrate, 4-methylumbelliferone-alpha-D-galactoside, w...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.45.2.622-627.1983
更新日期:1983-02-01 00:00:00
abstract::Rolling circle amplification (RCA) generates large single-stranded and tandem repeats of target DNA as amplicons. This technique was applied to in situ nucleic acid amplification (in situ RCA) to visualize and count single Escherichia coli cells carrying a specific gene sequence. The method features (i) one short targ...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.71.12.7933-7940.2005
更新日期:2005-12-01 00:00:00
abstract::A push-pull method, previously used in groundwater analyses, was successfully adapted for measuring sulfide turnover rates in situ at different depths in the meromictic Lake Cadagno. In the layer of phototrophic bacteria at about 12 m in depth net sulfide consumption was observed during the day, indicating active bact...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/aem.66.2.712-717.2000
更新日期:2000-02-01 00:00:00
abstract::Light-dependent regulation of cell cycle progression in the marine cyanobacterium Synechococcus strain WH-8101 was demonstrated through the use of flow cytometry. Our results show that, similar to eucaryotic cells, marine Synechococcus spp. display two gaps in DNA synthesis, at the beginning and at the end of the cell...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.55.2.425-432.1989
更新日期:1989-02-01 00:00:00
abstract::We reported previously on the function of acyl coenzyme A (acyl-CoA) oxidase isozymes in the yeast Yarrowia lipolytica by investigating strains disrupted in one or several acyl-CoA oxidase-encoding genes (POX1 through POX5) (H. Wang et al., J. Bacteriol. 181:5140-5148, 1999). Here, these mutants were studied for lacto...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/aem.66.3.1233-1236.2000
更新日期:2000-03-01 00:00:00
abstract::A soil bacterial consortium that was grown on diesel fuel and consisted of more than 10 members from different genera was maintained through repetitive subculturing and was utilized as a practical model to investigate a bacterial community that was continuously exposed to petroleum hydrocarbons. Through metagenomics a...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.02268-20
更新日期:2020-12-17 00:00:00
abstract::The ferric uptake regulator (Fur) box-like sequence was located upstream of the serine protease-encoding gene (aprII) from a marine bacterium, Alteromonas sp. strain O-7. To clarify whether the production of AprII (the gene product of aprII) is regulated by the environmental iron concentrations, this strain was cultur...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/aem.66.9.3778-3783.2000
更新日期:2000-09-01 00:00:00
abstract::Here, we report cloning of cyanobacterial genes encoding pteridine glycosyltransferases that catalyze glucosyl or xylosyl transfer from UDP-sugars to tetrahydrobiopterin. The genes were cloned by PCR amplification from genomic DNA which was isolated from culture and environmental samples and overexpressed in Escherich...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.01083-10
更新日期:2010-11-01 00:00:00
abstract::In this study, the effect of individual lecithin phospholipids on the antimicrobial properties of eugenol against Escherichia coli C600 was investigated. We tested five major phospholipids common in soy or egg lecithin (1,2-dihexadecanoyl-sn-glycero-3-phosphocholine [DPPC], 1,2-dioctadecanoyl-sn-glycero-3-phosphocholi...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.01583-17
更新日期:2017-10-17 00:00:00
abstract::The methylotrophic yeast Pichia pastoris has been utilized for heterologous protein expression for over 30 years. Because P. pastoris secretes few of its own proteins, the exported recombinant protein is the major polypeptide in the extracellular medium, making purification relatively easy. Unfortunately, some recombi...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.01615-19
更新日期:2019-11-27 00:00:00
abstract::Salmonella enterica serovar Enteritidis that grows to a higher cell density (SE-HCD) than wild type while retaining O-chain lipopolysaccharide was isolated by transforming wild type serovar Enteritidis with the cell density sensor plasmid pSB402 and selecting for bioluminescence. A luminescent strain, SE-HCD, that emi...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.64.6.2166-2172.1998
更新日期:1998-06-01 00:00:00
abstract::Bacterial Halanaerobium strains become the dominant persisting microbial community member in produced fluids across geographically distinct hydraulically fractured shales. Halanaerobium is believed to be inadvertently introduced into this environment during the drilling and fracturing process and must therefore tolera...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.00018-19
更新日期:2019-05-30 00:00:00
abstract::The distribution, architecture, and conjugal capacity of nisin-sucrose elements in wild-type Lactococcus lactis strains were studied. Element architecture was analyzed with the aid of hybridizations to different probes derived from the nisin-sucrose transposon Tn5276 of L. lactis NIZO R5, including its left and right ...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.60.6.1798-1804.1994
更新日期:1994-06-01 00:00:00
abstract::A new method for the rapid and sensitive detection of Legionella pneumophila in hot water systems has been developed. The method is based on an IF assay combined with detection by solid-phase cytometry. This method allowed the enumeration of L. pneumophila serogroup 1 and L. pneumophila serogroups 2 to 6, 8 to 10, and...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/aem.70.3.1651-1657.2004
更新日期:2004-03-01 00:00:00
abstract::Bacterioplankton community diversity was investigated in the subtropical Brisbane River-Moreton Bay estuary, Australia (27 degrees 25 minutes S, 153 degrees 5 minutes E). Bacterial communities were studied using automated rRNA intergenic spacer analysis (ARISA), which amplifies 16S-23S ribosomal DNA internally transcr...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.70.6.3425-3433.2004
更新日期:2004-06-01 00:00:00
abstract::The complete nucleotide sequences of two alleles of cellobiose dehydrogenase, cdh-1 (3,627 bp) and cdh-2 (3,623 bp), from Phanerochaete chrysosporium OGC101 are reported. The nucleotide sequences of cdh-1 and cdh-2 exhibit 97% similarity. A total of eighty-six point mutations between cdh-1 and cdh-2 are observed. Both...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.63.2.796-799.1997
更新日期:1997-02-01 00:00:00
abstract::Pyrobaculum islandicum uses iron, thiosulfate, and elemental sulfur for anaerobic respiration, while Pyrobaculum aerophilum uses iron and nitrate; however, the constraints on these processes and their physiological mechanisms for iron and sulfur reduction are not well understood. Growth rates on sulfur compounds are h...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.02033-07
更新日期:2008-01-01 00:00:00
abstract::A membrane filtration method incorporating a combination of anaerobic and aerobic incubation has been developed for the enumeration of Aeromonas spp. in drinking water. The use of anaerobic incubation improved the detection of Aeromonas spp. by reducing the growth of nonaeromonads. The confirmation rate of presumptive...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.55.9.2138-2140.1989
更新日期:1989-09-01 00:00:00
abstract::The interactive effect of phosphate-solubilizing bacteria and arbuscular mycorrhizal (AM) fungi on plant use of soil P sources of low bioavailability (endogenous or added as rock phosphate [RP] material) was evaluated by using soil microcosms which integrated (sup32)P isotopic dilution techniques. The microbial inocul...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.63.11.4408-4412.1997
更新日期:1997-11-01 00:00:00
abstract::An acclimated shake flask CO(2) evolution test was used to study the biodegradability of 14 commercial phthalate esters that are commonly used as plasticizers. Both CO(2) evolution (ultimate biodegradation) and loss of parent phthalate esters (primary biodegradation) were measured. With only a few exceptions, primary ...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.47.4.601-606.1984
更新日期:1984-04-01 00:00:00
abstract::Since Vibrio cholerae O139 first appeared in 1992, both O1 El Tor and O139 have been recognized as the epidemic serogroups, although their geographic distribution, endemicity, and reservoir are not fully understood. To address this lack of information, a study of the epidemiology and ecology of V. cholerae O1 and O139...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.00066-06
更新日期:2006-06-01 00:00:00
abstract::A strain that efficiently degraded methyl tert-butyl ether (MTBE) was obtained by initial selection on the recalcitrant compound tert-butyl alcohol (TBA). This strain, a gram-positive methylotrophic bacterium identified as Mycobacterium austroafricanum IFP 2012, was also able to degrade tert-amyl methyl ether and tert...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/aem.68.6.2754-2762.2002
更新日期:2002-06-01 00:00:00
abstract::To gain a predictive understanding of the interspecies interactions within microbial communities that govern community function, the genomic complement of every member population must be determined. Although metagenomic sequencing has enabled the de novo reconstruction of some microbial genomes from environmental comm...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.02274-15
更新日期:2015-10-23 00:00:00
abstract::A novel single-step procedure for the purification of staphylococcal enterotoxin A (SEA), namely, dye ligand affinity chromatography with the triazine dye Red A, was developed. SEA purified by this method produced a single band when subjected to sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The yield from...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.54.7.1761-1765.1988
更新日期:1988-07-01 00:00:00
abstract::A novel method of detecting extracellular protease activity at biofilm-substratum interfaces was developed. This method utilizes fluorescent molecules bound to cellulose substrata with a lectin. Extracellular proteases degrade the lectin and release the fluorochrome into solution. This new technique and a standard dis...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/aem.67.9.4329-4334.2001
更新日期:2001-09-01 00:00:00
abstract::We developed a chemiluminometric assay of beta-galactosidase in coliform bacteria, using a phenylgalactose-substituted 1,2-dioxetane derivative as a substrate. Permeabilization of cells is required to ensure the efficient cellular uptake of this compound. By this method, one coliform seeded in 100 ml of sterile water ...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.61.12.4505-4509.1995
更新日期:1995-12-01 00:00:00
abstract::Dextransucrase (DSR-S) from Leuconostoc mesenteroides NRRL B-512F is a glucosyltransferase that catalyzes synthesis of soluble dextran from sucrose. In the presence of efficient acceptor molecules, such as maltose, the reaction pathway is shifted toward glucooligosaccharide synthesis. Like glucosyltransferases from or...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.64.5.1644-1649.1998
更新日期:1998-05-01 00:00:00
abstract::Studies of time-dependent drug and environmental effects on single, live bacterial cells would benefit significantly from a permeable, nonperturbative, long-lived fluorescent stain specific to the nucleoids (chromosomal DNA). The ideal stain would not affect cell growth rate or nucleoid morphology and dynamics, even d...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.00989-14
更新日期:2014-08-01 00:00:00