Development of an amylolytic Lactobacillus plantarum silage strain expressing the Lactobacillus amylovorus alpha-amylase gene.

abstract：:Rumen-protected glucose (RPG) plays an important role in alleviating the negative energy balance of dairy cows. This study used a combination of rumen microbes 16S and metabolomics to elucidate the changes of rumen microbial composition and rumen metabolites of different doses of RPG's rumen degradation part in early-...

journal_title：Applied and environmental microbiology

authors： Wang Y,Nan X,Zhao Y,Wang Y,Jiang L,Xiong B

更新日期：2021-01-04 00:00:00

abstract：:This study was initiated to determine if reduced sulfur contained in coal gasifier ash and slag was available for microbial and chemical oxidation because eventual large-quantity landfill disposal of these solid wastes is expected. Continuous application of distilled water to a column containing a high-sulfur-content ...

journal_title：Applied and environmental microbiology

authors： Strayer RF,Davis EC

更新日期：1983-03-01 00:00:00

abstract：:Glucose metabolism and the mechanisms of NADH oxidation by Treponema hyodysenteriae were studied. Under an N2 atmosphere, washed cell suspensions of the spirochete consumed glucose and produced acetate, butyrate, H2, and CO2. Approximately twice as much H2 as CO2 was produced. Determinations of radioactivity in produc...

journal_title：Applied and environmental microbiology

authors： Stanton TB

更新日期：1989-09-01 00:00:00

abstract：:The use of bioluminescence as a sensitive marker for detection of Pseudomonas spp. in the rhizosphere was investigated. Continuous expression of the luxCDABE genes, required for bioluminescence, was not detectable in the rhizosphere. However, when either a naphthalene-inducible luxCDABE construct or a constitutive lux...

journal_title：Applied and environmental microbiology

authors： de Weger LA,Dunbar P,Mahafee WF,Lugtenberg BJ,Sayler GS

更新日期：1991-12-01 00:00:00

abstract：:The MK filter is an electropositively charged filter that can be used to concentrate enteroviruses from large volumes (400 to 1,000 liters) of water. This filter is less expensive than the commonly used 1MDS electropositive filter. In this study, we compared the recovery of poliovirus 1 (PV1) and that of coxsackieviru...

journal_title：Applied and environmental microbiology

authors： Ma JF,Naranjo J,Gerba CP

更新日期：1994-06-01 00:00:00

abstract：:The importance of viruses in aquatic ecosystem functioning has been widely described. However, few studies have examined tropical aquatic ecosystems. Here, we evaluated for the first time viruses and their relationship with other planktonic communities in an Amazonian freshwater ecosystem. Coupling between viruses and...

journal_title：Applied and environmental microbiology

authors： Barros N,Farjalla VF,Soares MC,Melo RC,Roland F

更新日期：2010-11-01 00:00:00

abstract：:Noroviruses, which are members of the Caliciviridae family, represent the leading cause of nonbacterial gastroenteritis in developed countries; such norovirus infections result in high economic costs for health protection. Person-to-person contact, contaminated water, and foods, especially raw shellfish, vegetables, a...

journal_title：Applied and environmental microbiology

authors： Buckow R,Isbarn S,Knorr D,Heinz V,Lehmacher A

更新日期：2008-02-01 00:00:00

abstract：:The pathways responsible for cell wall polysaccharide biosynthesis are vital in eukaryotic microorganisms. The corresponding synthases are potential targets of inhibitors such as fungicides. Despite their fundamental and economical importance, most polysaccharide synthases are not well characterized, and their molecul...

journal_title：Applied and environmental microbiology

authors： Briolay A,Bouzenzana J,Guichardant M,Deshayes C,Sindt N,Bessueille L,Bulone V

更新日期：2009-04-01 00:00:00

abstract：:In many cities, the feral rock dove is an abundant bird species that can harbor Chlamydophila psittaci. We determined the prevalence and genotype of C. psittaci in fresh fecal samples from feral pigeons in Amsterdam, The Netherlands. The prevalence was 7.9% overall (26/331; 95% confidence interval, 5 to 11). Ten genot...

journal_title：Applied and environmental microbiology

authors： Heddema ER,Ter Sluis S,Buys JA,Vandenbroucke-Grauls CM,van Wijnen JH,Visser CE

更新日期：2006-06-01 00:00:00

abstract：:The chromate reductase purified from Pseudomonas ambigua was found to be homologous with several nitroreductases. Escherichia coli DH5alpha and Vibrio harveyi KCTC 2720 nitroreductases were chosen for the present study, and their chromate-reducing activities were determined. A fusion between glutathione S-transferase ...

journal_title：Applied and environmental microbiology

authors： Kwak YH,Lee DS,Kim HB

更新日期：2003-08-01 00:00:00

abstract：:To produce high levels of β-lactams, the filamentous fungus Penicillium rubens (previously named Penicillium chrysogenum) has been subjected to an extensive classical strain improvement (CSI) program during the last few decades. This has led to the accumulation of many mutations that were spread over the genome. Detai...

journal_title：Applied and environmental microbiology

authors： Wu M,Crismaru CG,Salo O,Bovenberg RAL,Driessen AJM

更新日期：2020-01-21 00:00:00

abstract：:The bacterial host range of coliphage P1 was extented by using the heat-inducible phage P1clr100KM. A gene for kanamycin resistance was transferred from Escherichia coli to members of the family Enterobacteriaceae and some other genera of gram-negative bacteria. P1 phage was produced by thermal induction from the lyso...

journal_title：Applied and environmental microbiology

authors： Murooka Y,Harada T

更新日期：1979-10-01 00:00:00

abstract：:An aminotransferase capable of transaminating 2-oxo-4-[(hydroxy)(methyl)phosphinoyl]butyric acid to L-phosphinothricin [L-homoalanine-4-yl-(methyl)phosphinic acid], the active ingredient of the herbicide Basta (Hoechst AG), was purified to apparent homogeneity from Escherichia coli K-12. The enzyme catalyzes the trans...

journal_title：Applied and environmental microbiology

authors： Schulz A,Taggeselle P,Tripier D,Bartsch K

更新日期：1990-01-01 00:00:00

abstract：:The reduction of cytotoxicity in virus concentrates from environmental samples was accomplished by high-speed centrifugation and by filtration of the samples through positively charged depth filters. ...

journal_title：Applied and environmental microbiology

authors： Hejkal TW,Gerba CP,Rao VC

更新日期：1982-03-01 00:00:00

abstract：:Vegetative cells of Bacillus cereus were subjected to low doses of nisin (0.06 microg/ml) and mild pulsed-electric field treatment (16.7 kV/cm, 50 pulses each of 2-micros duration). Combining both treatments resulted in a reduction of 1.8 log units more than the sum of the reductions obtained with the single treatment...

journal_title：Applied and environmental microbiology

authors： Pol IE,Mastwijk HC,Bartels PV,Smid EJ

更新日期：2000-01-01 00:00:00

abstract：:A multilocus enzyme electrophoresis analysis of 96 strains of the salmonellae distinguished 80 electrophoretic types (ETs) and placed them in eight groups, seven of which correspond precisely to the seven taxonomic groups (I, II, IIIa, IIIb, IV, V, and VI) previously defined on the basis of biotype and genomic DNA hyb...

journal_title：Applied and environmental microbiology

authors： Boyd EF,Wang FS,Whittam TS,Selander RK

更新日期：1996-03-01 00:00:00

abstract：:Despite a wealth of studies examining the toxicity of engineered nanomaterials, current knowledge on their cytotoxic mechanisms (particularly from a physical perspective) remains limited. In this work, we imaged and quantitatively characterized the biomechanical (hardness and elasticity), adhesive, and surface electri...

journal_title：Applied and environmental microbiology

authors： Zhang W,Hughes J,Chen Y

更新日期：2012-06-01 00:00:00

abstract：:Multiple anthropogenic disturbances to bacterial diversity have been investigated in coastal ecosystems, in which temporal variability in the bacterioplankton community has been considered a ubiquitous process. However, far less is known about the temporal dynamics of a bacterioplankton community responding to polluti...

journal_title：Applied and environmental microbiology

authors： Wang K,Zhang D,Xiong J,Chen X,Zheng J,Hu C,Yang Y,Zhu J

更新日期：2015-01-01 00:00:00

abstract：:[This corrects the article on p. 1239 in vol. 45.]. ...

journal_title：Applied and environmental microbiology

authors：

更新日期：1983-07-01 00:00:00

abstract：:Salmonella enterica serovar Enteritidis that grows to a higher cell density (SE-HCD) than wild type while retaining O-chain lipopolysaccharide was isolated by transforming wild type serovar Enteritidis with the cell density sensor plasmid pSB402 and selecting for bioluminescence. A luminescent strain, SE-HCD, that emi...

journal_title：Applied and environmental microbiology

authors： Guard-Petter J

更新日期：1998-06-01 00:00:00

abstract：:There is a wealth of evidence indicating that mobile genetic elements can spread in natural microbial communities. However, little is known regarding the fraction of the community that actually engages in this behavior. Here we report on a new approach to quantify the fraction of a bacterial community that is able to ...

journal_title：Applied and environmental microbiology

authors： Musovic S,Dechesne A,Sørensen J,Smets BF

更新日期：2010-07-01 00:00:00

abstract：:The inactivation of catechol 2,3-dioxygenase from Pseudomonas putida mt-2 by 3-chloro- and 3-fluorocatechol and the iron-chelating agent Tiron (catechol-3,5-disulfonate) was studied. Whereas inactivation by Tiron is an oxygen-independent and mostly reversible process, inactivation by the 3-halocatechols was only obser...

journal_title：Applied and environmental microbiology

authors： Bartels I,Knackmuss HJ,Reineke W

更新日期：1984-03-01 00:00:00

abstract：:Ceftiofur, a third-generation cephalosporin antimicrobial, was used in Canadian hatcheries for many years to prevent early mortality in chicks, leading to a high prevalence of cephalosporin resistance in Escherichia coli in chickens. Preventive use of ceftiofur in hatcheries ceased in 2014. We examined the effect of c...

journal_title：Applied and environmental microbiology

authors： Verrette L,Fairbrother JM,Boulianne M

更新日期：2019-06-17 00:00:00

abstract：:Staphylococcus aureus is a common hospital and household pathogen. Given the emergence of antibiotic-resistant derivatives of this pathogen resulting from the use of antibiotics as general treatment, development of alternative therapeutic strategies is urgently needed. Here, we assess the feasibility of killing S. aur...

journal_title：Applied and environmental microbiology

authors： Chen C,Chen L,Yi Y,Chen C,Wu LF,Song T

更新日期：2016-02-12 00:00:00

abstract：:Legionella pneumophila, the agent of Legionnaires' disease, is an intracellular parasite of aquatic amoebae and human macrophages. A key factor for L. pneumophila in intracellular infection is its type II protein secretion system (Lsp). In order to more completely define Lsp output, we recently performed a proteomic a...

journal_title：Applied and environmental microbiology

authors： Rossier O,Dao J,Cianciotto NP

更新日期：2008-02-01 00:00:00

abstract：:Quantitative assessment of growth of filamentous microorganisms, such as streptomycetes, is generally restricted to determination of dry weight. Here, we describe a straightforward methylene blue-based sorption assay to monitor microbial growth quantitatively, simply, and rapidly. The assay is equally applicable to un...

journal_title：Applied and environmental microbiology

authors： Fischer M,Sawers RG

更新日期：2013-07-01 00:00:00

abstract：:The luminescence signal of luxAB-encoded bacterial luciferase strongly depends on the metabolic state of the host cell, which restricts the use of this reporter system to metabolically active bacteria. Here we show that in stationary-phase cells of Lactococcus lactis, detection of luciferase is significantly improved ...

journal_title：Applied and environmental microbiology

authors： Bachmann H,Santos F,Kleerebezem M,van Hylckama Vlieg JE

更新日期：2007-07-01 00:00:00

abstract：:The effects of antibiotic production on rhizosphere microbial communities of field-grown Phaseolus vulgaris were assessed by using ribosomal intergenic spacer analysis. Inoculum strains of Rhizobium etli CE3 differing only in trifolitoxin production were used. Trifolitoxin production dramatically reduced the diversity...

journal_title：Applied and environmental microbiology

authors： Robleto EA,Borneman J,Triplett EW

更新日期：1998-12-01 00:00:00

abstract：:We evaluated the restriction fragment length polymorphism of genomic DNA among 53 strains of the phytopathogenic bacterium Pseudomonas syringae pv. syringae. Twenty-nine strains were isolated from beans, and the rest were isolated from 11 other hosts. Southern blots of DNA digested with EcoRI or HindIII were hybridize...

journal_title：Applied and environmental microbiology

authors： Legard DE,Aquadro CF,Hunter JE

更新日期：1993-12-01 00:00:00

abstract：:Standard plate count (SPC) bacteria were isolated from a drinking-water treatment facility and from the river supplying the facility. All isolates were identified and tested for their resistance to six antibiotics to determine if drug-resistant bacteria were selected for as a consequence of water treatment. Among the ...

journal_title：Applied and environmental microbiology

authors： Armstrong JL,Calomiris JJ,Seidler RJ

更新日期：1982-08-01 00:00:00