Reduction of cytotoxicity in virus concentrates from environmental samples.

abstract：:Histoplasma capsulatum is very prevalent in the environment and is one of the most common causes of mycoses in humans and diverse animals in Brazil. Multiple typing methods have been developed to study H. capsulatum epidemiology; however, there is limited information concerning comparisons of results obtained with dif...

journal_title：Applied and environmental microbiology

authors： Muniz Mde M,Morais E Silva Tavares P,Meyer W,Nosanchuk JD,Zancope-Oliveira RM

更新日期：2010-07-01 00:00:00

abstract：UNLABELLED:By combining genomics and isotope imaging analysis using high-resolution secondary ion mass spectrometry (NanoSIMS), we examined the function and evolution of Bacteroidales ectosymbionts of the protist Barbulanympha from the hindguts of the wood-eating cockroach Cryptocercus punctulatus In particular, we inv...

journal_title：Applied and environmental microbiology

authors： Tai V,Carpenter KJ,Weber PK,Nalepa CA,Perlman SJ,Keeling PJ

更新日期：2016-07-15 00:00:00

abstract：:Heterologous protein production can be doubled by increasing the copy number of the corresponding heterologous gene. We constructed a host-vector system in the yeast Kluyveromyces lactis that was able to induce copy number amplification of pKD1 plasmid-based vectors upon expression of an integrated copy of the plasmid...

journal_title：Applied and environmental microbiology

authors： Morlino GB,Tizzani L,Fleer R,Frontali L,Bianchi MM

更新日期：1999-11-01 00:00:00

abstract：:We determined the effects of grain size and nutritional conditions on the penetration rate and metabolic activity of Escherichia coli strains in anaerobic, nutrient-saturated chambers packed with different sizes of glass beads (diameters, 116 to 767 mum) under static conditions. The chambers had nearly equal porositie...

journal_title：Applied and environmental microbiology

authors： Sharma PK,McInerney MJ

更新日期：1994-05-01 00:00:00

abstract：:O-antigen biosynthetic (wbf) regions for Vibrio cholerae serogroups O5, O8, and O108 were isolated and sequenced. Sequences were compared to those of other published V. cholerae O-antigen regions. These wbf regions showed a high degree of heterogeneity both in gene content and in gene order. Genes identified frequentl...

journal_title：Applied and environmental microbiology

authors： Aydanian A,Tang L,Morris JG,Johnson JA,Stine OC

更新日期：2011-04-01 00:00:00

abstract：:Our previous genetic analysis (J. W. Willis, J. K. Engwall, and A. K. Chatterjee, Phytopathology 77:1199-1205, 1987) had revealed a tight linkage between pel-3 (pel, pectate lyase gene) and peh-1 (peh, polygalacturonase gene) within the chromosome of Erwinia carotovora subsp. carotovora 71. Nucleotide sequencing, tran...

journal_title：Applied and environmental microbiology

authors： Liu Y,Chatterjee A,Chatterjee AK

更新日期：1994-07-01 00:00:00

abstract：:Rhodococcus erythropolis BG43 is able to degrade the Pseudomonas aeruginosa quorum sensing signal molecules PQS (Pseudomonas quinolone signal) [2-heptyl-3-hydroxy-4(1H)-quinolone] and HHQ [2-heptyl-4(1H)-quinolone] to anthranilic acid. Based on the hypothesis that degradation of HHQ might involve hydroxylation to PQS ...

journal_title：Applied and environmental microbiology

authors： Müller C,Birmes FS,Rückert C,Kalinowski J,Fetzner S

更新日期：2015-11-01 00:00:00

abstract：:Enrichment was performed to isolate organisms that could utilize reduced phosphorus compounds as their sole phosphorus sources. One isolate that grew well with either hypophosphite or phosphite was identified by 16S rRNA gene analysis as a strain of Alcaligenes faecalis. The genes required for oxidation of hypophosphi...

journal_title：Applied and environmental microbiology

authors： Wilson MM,Metcalf WW

更新日期：2005-01-01 00:00:00

abstract：:Low-temperature ecosystems represent the largest biosphere on Earth, and yet our understanding of the roles of bacteriophages in these systems is limited. Here, the influence of the cold-active filamentous phage SW1 on the phenotype and gene transcription of its host, Shewanella piezotolerans WP3 (WP3), was investigat...

journal_title：Applied and environmental microbiology

authors： Jian H,Xiao X,Wang F

更新日期：2013-11-01 00:00:00

abstract：:The 3-hydroxy fatty acids (3-OHFAs) in lipopolysaccharides (LPS) play an important role in determining endotoxin activity, and childhood exposure to endotoxin has recently been associated with reduced risk of atopic diseases. To characterize the 3-OHFAs in house dust (HD), we used gas chromatography-mass spectrometry ...

journal_title：Applied and environmental microbiology

authors： Park JH,Szponar B,Larsson L,Gold DR,Milton DK

更新日期：2004-01-01 00:00:00

abstract：:The binding affinities and specificities of six truncated S-layer homology domain (SLH) polypeptides of mosquitocidal Bacillus sphaericus strain C3-41 with the purified cell wall sacculi have been assayed. The results indicated that the SLH polypeptide comprised of amino acids 31 to 210 was responsible for anchoring t...

journal_title：Applied and environmental microbiology

authors： Li J,Hu X,Yan J,Yuan Z

更新日期：2009-06-01 00:00:00

abstract：:A purified lipase from the yeast Cryptococcus sp. strain S-2 exhibited remote homology to proteins belonging to the cutinase family rather than to lipases. This enzyme could effectively degrade the high-molecular-weight compound polylactic acid, as well as other biodegradable plastics, including polybutylene succinate...

journal_title：Applied and environmental microbiology

authors： Masaki K,Kamini NR,Ikeda H,Iefuji H

更新日期：2005-11-01 00:00:00

abstract：:In this study, host-associated molecular markers and bacterial 16S rRNA gene community analysis using high-throughput sequencing were used to identify the sources of fecal pollution in environmental waters in Brisbane, Australia. A total of 92 fecal and composite wastewater samples were collected from different host g...

journal_title：Applied and environmental microbiology

authors： Ahmed W,Staley C,Sadowsky MJ,Gyawali P,Sidhu JP,Palmer A,Beale DJ,Toze S

更新日期：2015-10-01 00:00:00

abstract：:A full-scale study evaluating an inoculum addition to stimulate in situ bioremediation of oily-sludge-contaminated soil was conducted at an oil refinery where the indigenous population of hydrocarbon-degrading bacteria in the soil was very low (10(3) to 10(4) CFU/g of soil). A feasibility study was conducted prior to ...

journal_title：Applied and environmental microbiology

authors： Mishra S,Jyot J,Kuhad RC,Lal B

更新日期：2001-04-01 00:00:00

abstract：:We applied a multilocus phylogenetic approach to elucidate the origin of serradella and lupin Bradyrhizobium strains that persist in soils of Western Australia and South Africa. The selected strains belonged to different randomly amplified polymorphic DNA (RAPD)-PCR clusters that were distinct from RAPD clusters of ap...

journal_title：Applied and environmental microbiology

authors： Stepkowski T,Moulin L,Krzyzańska A,McInnes A,Law IJ,Howieson J

更新日期：2005-11-01 00:00:00

abstract：:Phomenoic acid and phomenolactone, previously isolated from the fungus Phoma lingam (Tode) Desm., have shown moderate antifungal and antibacterial properties in vitro. To rationalize the production of phomenoic acid, a kinetic study of its biosynthesis in the mycelium was performed. Phomenoic acid and phomenolactone a...

journal_title：Applied and environmental microbiology

authors： Topgi RS,Devys M,Bousquet JF,Kollmann A,Barbier M

更新日期：1987-05-01 00:00:00

abstract：:A gene fragment belonging to the cytochrome P-450 superfamily has been cloned and identified from stationary cultures of the filamentous fungus Phanerochaete chrysosporium by reverse transcriptase (RT)-PCR. A set of degenerate primers homologous to highly conserved regions of known cytochrome P-450 sequences were used...

journal_title：Applied and environmental microbiology

authors： Kullman SW,Matsumura F

更新日期：1997-07-01 00:00:00

abstract：:Vibrio cholerae O1 strain TSI-4 (El Tor, Ogawa) can shift to a rugose colony morphology from its normal translucent colony morphology in response to nutrient starvation. We have investigated differences between the rugose and translucent forms of V. cholerae O1 strain TSI-4. Electron microscopic examination of the rug...

journal_title：Applied and environmental microbiology

authors： Wai SN,Mizunoe Y,Takade A,Kawabata SI,Yoshida SI

更新日期：1998-10-01 00:00:00

abstract：:The ability of terminal restriction fragment (T-RFLP or TRF) profiles of 16S rRNA genes to provide useful information about the relative diversity of complex microbial communities was investigated by comparison with other methods. Four soil communities representing two pinyon rhizosphere and two between-tree (interspa...

journal_title：Applied and environmental microbiology

authors： Dunbar J,Ticknor LO,Kuske CR

更新日期：2000-07-01 00:00:00

abstract：:Simkania negevensis is a recently discovered chlamydia-like intracellular microorganism which has been associated with bronchiolitis in infants and with community-acquired pneumonia in adults; a high seroprevalence of antibodies to the microorganism has been found in various population groups. S. negevensis can be gro...

journal_title：Applied and environmental microbiology

authors： Kahane S,Platzner N,Dvoskin B,Itzhaki A,Friedman MG

更新日期：2004-06-01 00:00:00

abstract：:A field evaluation of double violet agar for the isolation and presumptive identification of Klebsiella pneumoniae from water has been performed. Water from the North Oconee River, Clarke County, Ga., was cultured for presence of klebsiellae using the membrane filter technique. Colonies were presumptively identified a...

journal_title：Applied and environmental microbiology

authors： Campbell LM,Roth IL,Klein RD

更新日期：1976-02-01 00:00:00

abstract：:A plasmid marker rescue system based on restoration of the nptII gene was established in Streptococcus gordonii to study the transfer of bacterial and transgenic plant DNA by transformation. In vitro studies revealed that the marker rescue efficiency depends on the type of donor DNA. Plasmid and chromosomal DNA of bac...

journal_title：Applied and environmental microbiology

authors： Kharazmi M,Sczesny S,Blaut M,Hammes WP,Hertel C

更新日期：2003-10-01 00:00:00

abstract：:Relative to those of unexposed cultures, the transcript levels of the four CprA-type reductive dehalogenase genes (cprA2, cprA3, cprA4, and cprA5) in Desulfitobacterium hafniense PCP-1 were measured in cultures exposed to chlorophenols. In 2,4,6-trichlorophenol-amended cultures, cprA2 and cprA3 were upregulated, as wa...

journal_title：Applied and environmental microbiology

authors： Bisaillon A,Beaudet R,Lépine F,Villemur R

更新日期：2011-09-01 00:00:00

abstract：:With the submersible JAGO and by scuba diving we discovered three remarkable geothermal cones, rising 33, 25, and 45 m from the seafloor at a depth of 65 m in Eyjafjordur, northern Iceland. The greatest geothermal activity was on the highest cone, which discharged up to 50 liters of freshwater per s at 72 degrees C an...

journal_title：Applied and environmental microbiology

authors： Marteinsson VT,Kristjánsson JK,Kristmannsdóttir H,Dahlkvist M,Saemundsson K,Hannington M,Pétursdóttir SK,Geptner A,Stoffers P

更新日期：2001-02-01 00:00:00

abstract：:The preparation of DNA by alcohol precipitation in the presence of Na1 was used to enable the direct detection of Listeria monocytogenes in the amount of 10(3) CFU/0.5 g of sample. This procedure produces PCR-quality DNA directly from foods, such as soft cheese and minced meat. ...

journal_title：Applied and environmental microbiology

authors： Makino S,Okada Y,Maruyama T

更新日期：1995-10-01 00:00:00

abstract：:Enterotoxigenic Escherichia coli (ETEC) strains that produce immunologically heterogeneous fimbriae and enterotoxins are the primary cause of neonatal diarrhea and postweaning diarrhea in young pigs. A multivalent vaccine inducing protective immunity against ideally all ETEC fimbriae and enterotoxins could be effectiv...

journal_title：Applied and environmental microbiology

authors： Lu T,Moxley RA,Zhang W

更新日期：2019-05-16 00:00:00

abstract：:A molecular method based on restriction fragment length polymorphism (RFLP) of PCR-amplified fragments of the 23S rRNA gene was designed to rapidly identify Listeria strains to the species level. Two fragments (S1, 460 bp, and S2, 890 bp) were amplified from boiled DNA. S2 was cut with the restriction enzymes XmnI or ...

journal_title：Applied and environmental microbiology

authors： Paillard D,Dubois V,Duran R,Nathier F,Guittet C,Caumette P,Quentin C

更新日期：2003-11-01 00:00:00

abstract：:High-throughput sequencing of the taxonomically informative 16S rRNA gene provides a powerful approach for exploring microbial diversity. Here we compare the performances of two common "benchtop" sequencing platforms, Illumina MiSeq and Ion Torrent Personal Genome Machine (PGM), for bacterial community profiling by 16...

journal_title：Applied and environmental microbiology

authors： Salipante SJ,Kawashima T,Rosenthal C,Hoogestraat DR,Cummings LA,Sengupta DJ,Harkins TT,Cookson BT,Hoffman NG

更新日期：2014-12-01 00:00:00

abstract：:Novel variants of Bacillus thuringiensis were isolated from the phylloplane of deciduous and conifer trees as well as of other plants. These isolates displayed a range of toxicity towards Trichoplusia ni. Immunoblot and toxin protein analysis indicate that these strains included representatives of the three principal ...

journal_title：Applied and environmental microbiology

authors： Smith RA,Couche GA

更新日期：1991-01-01 00:00:00

abstract：:Pili (fimbriae) were observed on cells of each of the five strains of Bradyrhizobium japonicum and the one strain of Rhizobium trifolii examined. Pili on B. japonicum were about 4 nm in diameter and polarly expressed. Piliated cells were estimated by transmission electron microscopy and hydrophobic attachment to polys...

journal_title：Applied and environmental microbiology

authors： Vesper SJ,Bauer WD

更新日期：1986-07-01 00:00:00