Assessment of microbial diversity in four southwestern United States soils by 16S rRNA gene terminal restriction fragment analysis.

abstract：:"Candidatus Methanoperedens nitroreducens" is an archaeon that couples the anaerobic oxidation of methane to nitrate reduction. In natural and man-made ecosystems, this archaeon is often found at oxic-anoxic interfaces where nitrate, the product of aerobic nitrification, cooccurs with methane produced by methanogens. ...

journal_title：Applied and environmental microbiology

authors： Guerrero-Cruz S,Cremers G,van Alen TA,Op den Camp HJM,Jetten MSM,Rasigraf O,Vaksmaa A

更新日期：2018-11-30 00:00:00

abstract：:A spoilage organism isolated from turbid beer is described. The bacterium was gram negative, catalase negative, strictly anaerobic, and rod shaped, having flagella only on one side of the cell. The main metabolic product was propionic acid. In addition acetic, succinic, and lactic acids and acetoin were formed. Malona...

journal_title：Applied and environmental microbiology

authors： Haikara A,Penttilä L,Enari TM,Lounatmaa K

更新日期：1981-02-01 00:00:00

abstract：:The common reed (Phragmites australis), a cosmopolitan aquatic macrophyte, plays an important role in the structure and function of aquatic ecosystems. We compared bacterial community compositions (BCCs) and their assembly processes in the root-associated compartments (i.e., rhizosphere and endosphere) of reed and bul...

journal_title：Applied and environmental microbiology

authors： He R,Zeng J,Zhao D,Huang R,Yu Z,Wu QL

更新日期：2020-07-02 00:00:00

abstract：:Separating dietary fiber from other polysaccharides in digesta and feces is necessary to understand its mechanisms of action. A gravimetric method that separates fecal plant and bacterial matter based on size and density was evaluated and modified to determine the plant and bacterial mass of lyophilized whole and blen...

journal_title：Applied and environmental microbiology

authors： Cabotaje LM,López-Guisa JM,Shinnick FL,Marlett JA

更新日期：1990-06-01 00:00:00

abstract：:We have extended the technique of electroporation as a genetic tool for manipulating the Agrobacterium tumefaciens chromosome. We used this technique to introduce chromosomal DNA into recipient A. tumefaciens strains by electroporation and constructed isogenic chvE mutants that share the same chromosomal background bu...

journal_title：Applied and environmental microbiology

authors： Charles TC,Doty SL,Nester EW

更新日期：1994-11-01 00:00:00

abstract：:The ability of Shewanella decolorationis S12 to obtain energy for growth by coupling the oxidation of various electron donors to dissimilatory azoreduction was investigated. This microorganism can reduce a variety of azo dyes by use of formate, lactate, pyruvate, or H(2) as the electron donor. Furthermore, strain S12 ...

journal_title：Applied and environmental microbiology

authors： Hong Y,Xu M,Guo J,Xu Z,Chen X,Sun G

更新日期：2007-01-01 00:00:00

abstract：:Biofilm bacteria challenged with monochloramine retained significant respiratory activity, even though they could not be cultured on agar plates. Microbial colony counts on agar media declined by approximately 99.9% after 1 h of disinfection, whereas the number of bacteria stained by a fluorescent redox dye experience...

journal_title：Applied and environmental microbiology

authors： Stewart PS,Griebe T,Srinivasan R,Chen CI,Yu FP,deBeer D,McFeters GA

更新日期：1994-05-01 00:00:00

abstract：:Differentiation of the species within the genus Listeria is important for the food industry but only a few reliable methods are available so far. While a number of studies have used Fourier transform infrared (FTIR) spectroscopy to identify bacteria, the extraction of complex pattern information from the infrared spec...

journal_title：Applied and environmental microbiology

authors： Rebuffo CA,Schmitt J,Wenning M,von Stetten F,Scherer S

更新日期：2006-02-01 00:00:00

abstract：:Sourdough lactic acid bacteria were preliminarily screened for proteolytic activity by using a digest of albumin and globulin polypeptides as a substrate. Based on their hydrolysis profile patterns, Lactobacillus alimentarius 15M, Lactobacillus brevis 14G, Lactobacillus sanfranciscensis 7A, and Lactobacillus hilgardii...

journal_title：Applied and environmental microbiology

authors： Di Cagno R,De Angelis M,Lavermicocca P,De Vincenzi M,Giovannini C,Faccia M,Gobbetti M

更新日期：2002-02-01 00:00:00

abstract：:Arctic ground squirrels (Urocitellus parryii) are active for a scant 3 to 5 months of the year. During the active season, adult squirrels compete for mates, reproduce, and fatten in preparation for hibernation, while juvenile squirrels, weaned in early July, must grow and acquire sufficient fat to survive their first ...

journal_title：Applied and environmental microbiology

authors： Stevenson TJ,Buck CL,Duddleston KN

更新日期：2014-07-01 00:00:00

abstract：:An aminopeptidase was purified from cell extracts of Lactococcus lactis subsp. cremoris AM2 by ion-exchange chromatography. After electrophoresis of the purified enzyme in the presence or absence of sodium dodecyl sulfate, one protein band was detected. The enzyme was a 300-kilodalton hexamer composed of identical sub...

journal_title：Applied and environmental microbiology

authors： Neviani E,Boquien CY,Monnet V,Thanh LP,Gripon JC

更新日期：1989-09-01 00:00:00

abstract：:The industrially important organism Corynebacterium glutamicum has been characterized in recent years for its robust ability to assimilate aromatic compounds. In this study, C. glutamicum strain AS 1.542 was investigated for its ability to catabolize phenylacetic acid (PAA). The paa genes were identified; they are org...

journal_title：Applied and environmental microbiology

authors： Chen X,Kohl TA,Rückert C,Rodionov DA,Li LH,Ding JY,Kalinowski J,Liu SJ

更新日期：2012-08-01 00:00:00

abstract：:The killing of Listeria monocytogenes following exposure to low pH, organic acids, and osmotic stress was enhanced by the addition of 5% (vol/vol) ethanol. At pH 3, for example, the presence of this agent stimulated killing by more than 3 log units in 40 min of exposure. The rate of cell death at pH 3.0 was dependent ...

journal_title：Applied and environmental microbiology

authors： Barker C,Park SF

更新日期：2001-04-01 00:00:00

abstract：:The microbial production of biofuels and other added-value chemicals is often limited by the intrinsic toxicity of these compounds. The phasin PhaP from the soil bacterium Azotobacter sp. strain FA8 is a polyhydroxyalkanoate granule-associated protein that protects recombinant Escherichia coli against several kinds of...

journal_title：Applied and environmental microbiology

authors： Mezzina MP,Álvarez DS,Egoburo DE,Díaz Peña R,Nikel PI,Pettinari MJ

更新日期：2017-06-30 00:00:00

abstract：:Wax-coated capsules containing selective ingredients (brilliant green and oxgall) were added at the time of inoculation of most-probable-number media (modified lactose broths). The inhibitory ingredients gradually diffused from the capsules into the nonselective media, imparting selectivity to the media. Concentration...

journal_title：Applied and environmental microbiology

authors： Lanz WW,Hartman PA

更新日期：1976-11-01 00:00:00

abstract：:We report the construction of an Escherichia coli mutant that harbors two compatible plasmids and that is able to synthesize labeled 2-O-alpha-D-mannosyl-D-glycerate from externally added labeled mannose without the loss of specific isotopic enrichment. The strain carries a deletion in the manA gene, encoding phosphom...

journal_title：Applied and environmental microbiology

authors： Sampaio MM,Santos H,Boos W

更新日期：2003-01-01 00:00:00

abstract：:Rabbits have been increasingly linked to the persistence of paratuberculosis (Johne's disease) in domestic ruminants in the United Kingdom. The aims of this study were to determine the routes of intraspecies transmission of Mycobacterium avium subspecies paratuberculosis (MAP) in rabbits and to estimate the probabilit...

journal_title：Applied and environmental microbiology

authors： Judge J,Kyriazakis I,Greig A,Davidson RS,Hutchings MR

更新日期：2006-01-01 00:00:00

abstract：:The changes in motility, chemotactic responsiveness, and flagellation of Rhizobium meliloti RMB7201, L5-30, and JJ1c10 were analyzed after transfer of the bacteria to buffer with no available C, N, or phosphate. Cells of these three strains remained viable for weeks after transfer to starvation buffer (SB) but lost al...

journal_title：Applied and environmental microbiology

authors： Wei X,Bauer WD

更新日期：1998-05-01 00:00:00

abstract：:This work describes the purification and characterization of propionicin F, the first bacteriocin isolated from Propionibacterium freudenreichii. The bacteriocin has a bactericidal activity and is only active against strains of P. freudenreichii. Propionicin F appears to be formed through a processing pathway new to b...

journal_title：Applied and environmental microbiology

authors： Brede DA,Faye T,Johnsborg O,Odegård I,Nes IF,Holo H

更新日期：2004-12-01 00:00:00

abstract：:We constructed a 7.9-kilobase-pair recombinant shuttle plasmid, designated pHR106, by combining desired segments of three plasmids: an Escherichia coli plasmid (pSL100) which provides a multiple cloning site, a Clostridium perfringens plasmid (pJU122) which provides a clostridial origin of replication, and an E. coli ...

journal_title：Applied and environmental microbiology

authors： Roberts I,Holmes WM,Hylemon PB

更新日期：1988-01-01 00:00:00

abstract：:Algal blooms in lakes are often associated with anthropogenic eutrophication; however, they can occur without the human introduction of nutrients to a lake. A rare bloom of the alga Picocystis sp. strain ML occurred in the spring of 2016 at Mono Lake, a hyperalkaline lake in California, which was also at the apex of a...

journal_title：Applied and environmental microbiology

authors： Stamps BW,Nunn HS,Petryshyn VA,Oremland RS,Miller LG,Rosen MR,Bauer KW,Thompson KJ,Tookmanian EM,Waldeck AR,Loyd SJ,Johnson HA,Stevenson BS,Berelson WM,Corsetti FA,Spear JR

更新日期：2018-10-17 00:00:00

abstract：:A nested single-copy locus-based quantitative PCR (qPCR) assay and a multicopy locus-based qPCR assay were developed to estimate endophytic biomass of fungal root symbionts belonging to the Phialocephala fortinii sensu lato-Acephala applanata species complex (PAC). Both assays were suitable for estimation of endophyti...

journal_title：Applied and environmental microbiology

authors： Tellenbach C,Grünig CR,Sieber TN

更新日期：2010-09-01 00:00:00

abstract：:Helix alpha 4 of Bacillus thuringiensis Cry toxins is thought to play a critical role in the toxins' mode of action. Accordingly, single-site substitutions of many Cry1Aa helix alpha 4 amino acid residues have previously been shown to cause substantial reductions in the protein's pore-forming activity. Changes in prot...

journal_title：Applied and environmental microbiology

authors： Girard F,Vachon V,Préfontaine G,Marceau L,Schwartz JL,Masson L,Laprade R

更新日期：2009-01-01 00:00:00

abstract：:Pseudomonas putida KT2440, a saprophytic soil bacterium that colonizes the plant root, is a suitable microorganism for the removal of pollutants and a stable host for foreign genes used in biotransformation processes. Because of its potential use in agriculture and industry, we investigated the conditions for the opti...

journal_title：Applied and environmental microbiology

authors： Muñoz-Rojas J,Bernal P,Duque E,Godoy P,Segura A,Ramos JL

更新日期：2006-01-01 00:00:00

abstract：:"Fingerprints" of strains of methanol-oxidizing bacteria were obtained by exposing them to a set of chemicals which could stimulate or inhibit the growth. The chemicals gave quantitative results, which were used to calculate the similarities between the strains. The method has been used for establishing identity or no...

journal_title：Applied and environmental microbiology

authors： Kühn I

更新日期：1978-11-01 00:00:00

abstract：:An extracellular serine proteinase from Brevibacterium linens ATCC 9174 was purified to homogeneity. pH and temperature optima were 8.5 and 50(deg)C, respectively. The results for the molecular mass of the proteinase were 56 kDa by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and 126 kDa by gel filtration...

journal_title：Applied and environmental microbiology

authors： Rattray FP,Bockelmann W,Fox PF

更新日期：1995-09-01 00:00:00

abstract：:We present a phylogenetic analysis of nine strains of symbiotic nitrogen-fixing bacteria isolated from nodules of tagasaste (Chamaecytisus proliferus) and other endemic woody legumes of the Canary Islands, Spain. These and several reference strains were characterized genotypically at different levels of taxonomic reso...

journal_title：Applied and environmental microbiology

authors： Vinuesa P,Rademaker JL,de Bruijn FJ,Werner D

更新日期：1998-06-01 00:00:00

abstract：:Salmonella enterica contamination in foods is a significant concern for public health. When DNA detection methods are used for analysis of foods, one of the major concerns is false-positive results from the detection of dead cells. To circumvent this crucial issue, a TaqMan quantitative real-time RT-PCR (qRT-PCR) assa...

journal_title：Applied and environmental microbiology

authors： González-Escalona N,Hammack TS,Russell M,Jacobson AP,De Jesús AJ,Brown EW,Lampel KA

更新日期：2009-06-01 00:00:00

abstract：:Protease-negative variants were shown to outcompete the wild-type strains of Streptococcus cremoris E(8), HP, and Wg(2) at pH values higher than 6.0 in milk. For S. cremoris E(8) this process was studied in more detail. At lower pH values the wild type had a selective advantage. This pH-dependent selection was not fou...

journal_title：Applied and environmental microbiology

authors： Hugenholtz J,Splint R,Konings WN,Veldkamp H

更新日期：1987-02-01 00:00:00

abstract：:Xylella fastidiosa is a gram-negative, xylem-limited bacterium affecting economically important crops (e.g., grapevine, citrus, and coffee). The citrus variegated chlorosis (CVC) strain of X. fastidiosa is the causal agent of this severe disease of citrus in Brazil and represents the first plant-pathogenic bacterium f...

journal_title：Applied and environmental microbiology

authors： Monteiro PB,Teixeira DC,Palma RR,Garnier M,Bové JM,Renaudin J

更新日期：2001-05-01 00:00:00