Suitability of quantitative real-time PCR to estimate the biomass of fungal root endophytes.

abstract：:In vivo hydrolysis of inulin and sucrose was examined in selected yeasts of the genus Kluyveromyces. Cells, grown in sucrose-limited chemostat cultures, were subjected to treatments for the removal of inulinase, the enzyme responsible for the hydrolysis of both inulin and sucrose. The effects of these treatments were ...

journal_title：Applied and environmental microbiology

authors： Rouwenhorst RJ,Ritmeester WS,Scheffers WA,Van Dijken JP

更新日期：1990-11-01 00:00:00

abstract：:Current approaches used for dose-response modeling of low-dose exposures of pathogens rely on assumptions and extrapolations. These models are important for quantitative microbial risk assessment of food. A mechanistic framework has been advocated as an alternative approach for evaluating dose-response relationships. ...

journal_title：Applied and environmental microbiology

authors： Abe H,Koyama K,Koseki S

更新日期：2020-12-17 00:00:00

abstract：:This report demonstrates the applicability of a combination of matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) mass spectrometry (MS) and chemometrics for rapid and reliable identification of vegetative cells of the causative agent of anthrax, Bacillus anthracis. Bacillus cultures were prepared ...

journal_title：Applied and environmental microbiology

authors： Lasch P,Beyer W,Nattermann H,Stämmler M,Siegbrecht E,Grunow R,Naumann D

更新日期：2009-11-01 00:00:00

abstract：:L-threonine can be made by the amino acid-producing bacterium Corynebacterium glutamicum. However, in the course of this process, some of the L-threonine is degraded to glycine. We detected an aldole cleavage activity of L-threonine in crude extracts with an activity of 2.2 nmol min(-1) (mg of protein)(-1). In order t...

journal_title：Applied and environmental microbiology

authors： Simic P,Willuhn J,Sahm H,Eggeling L

更新日期：2002-07-01 00:00:00

abstract：:Suspensions of Nitrosomonas europaea catalyzed the oxidation of the commercial nitrification inhibitor nitrapyrin [2-chloro-6-(trichloromethyl)-pyridine]. Rapid oxidation of nitrapyrin (at a concentration of 10 muM) required the concomitant oxidation of ammonia, hydroxylamine, or hydrazine. The turnover rate was highe...

journal_title：Applied and environmental microbiology

authors： Vannelli T,Hooper AB

更新日期：1992-07-01 00:00:00

abstract：:On the basis of bioinformatic evidence, we suspected that proteins with a CYTH (CyaB thiamine triphosphatase) domain and/or a CHAD (conserved histidine α-helical domain) motif might represent polyphosphate (polyP) granule-associated proteins. We found no evidence of polyP targeting by proteins with CYTH domains. In co...

journal_title：Applied and environmental microbiology

authors： Tumlirsch T,Jendrossek D

更新日期：2017-03-17 00:00:00

abstract：:The variation in manure-amended soil survival capability among 18 Escherichia coli O157 strains (8 animal, 1 food, and 9 human isolates) was studied using a single sandy soil sample and a single sample of cattle manure as the inoculum carrier. The virulence profiles of E. coli O157 strains were characterized by detect...

journal_title：Applied and environmental microbiology

authors： Franz E,van Hoek AH,Bouw E,Aarts HJ

更新日期：2011-11-01 00:00:00

abstract：:White rot basidiomycetes were able to biodegrade styrene (1-phenylethene) graft copolymers of lignin containing different proportions of lignin and polystyrene [poly(1-phenylethylene)]. The biodegradation tests were run on lignin-styrene copolymerization products which contained 10.3, 32.2, and 50.4% (wt/wt) lignin. T...

journal_title：Applied and environmental microbiology

authors： Milstein O,Gersonde R,Huttermann A,Chen MJ,Meister JJ

更新日期：1992-10-01 00:00:00

abstract：:Monoclonal antibodies (MAbs) against Vibrio species that infect humans, fish, and shellfish were developed for application in rapid identifications. The pathogens included Vibrio alginolyticus, V. anguillarum, V. carchariae, V. cholerae, V. damsela, V. furnissii, V. harveyi, V. ordalii, V. parahaemolyticus, and V. vul...

journal_title：Applied and environmental microbiology

authors： Chen D,Hanna PJ,Altmann K,Smith A,Moon P,Hammond LS

更新日期：1992-11-01 00:00:00

abstract：:Anaerobic ammonium oxidation is a recent addition to the microbial nitrogen cycle, and its metabolic pathway, including the production and conversion of its intermediate hydrazine, is not well understood. Therefore, the effect of hydroxylamine addition on the hydrazine metabolism of anaerobic ammonium-oxidizing (anamm...

journal_title：Applied and environmental microbiology

authors： van der Star WR,van de Graaf MJ,Kartal B,Picioreanu C,Jetten MS,van Loosdrecht MC

更新日期：2008-07-01 00:00:00

abstract：:Reservoir 29 and Lake B are extremely acid lakes (epilimnion pHs of 2.7 and 3.2, respectively), because they receive acidic discharges from coal refuse piles. They differ in that the pH of profundal sediments in Reservoir 29 increased from 2.7 to 3.8 during the period of thermal stratification, whereas permanently ano...

journal_title：Applied and environmental microbiology

authors： Gyure RA,Konopka A,Brooks A,Doemel W

更新日期：1987-09-01 00:00:00

abstract：:Gamma irradiation is a nonthermal processing technology that has been used for the preservation of a variety of food products. This technology has been shown to effectively inactivate bacterial pathogens. Currently, the FDA has approved doses of up to 4.0 kGy to control food-borne pathogens in fresh iceberg lettuce an...

journal_title：Applied and environmental microbiology

authors： Feng K,Divers E,Ma Y,Li J

更新日期：2011-05-01 00:00:00

abstract：:Understanding the survival of fecal indicator bacteria (FIB) and microbial source-tracking (MST) markers is critical to developing pathogen fate and transport models. Although pathogen survival in water microcosms and manure-amended soils is well documented, little is known about their survival in intact cow pats depo...

journal_title：Applied and environmental microbiology

authors： Oladeinde A,Bohrmann T,Wong K,Purucker ST,Bradshaw K,Brown R,Snyder B,Molina M

更新日期：2014-01-01 00:00:00

abstract：:Achromobacter spp. are nonfermentative Gram-negative bacilli considered emergent pathogens in cystic fibrosis (CF). Although some cross-transmission events between CF patients have been described, Achromobacter strains were mostly patient specific, suggesting sporadic acquisitions from nonhuman reservoirs. However, so...

journal_title：Applied and environmental microbiology

authors： Dupont C,Jumas-Bilak E,Doisy C,Aujoulat F,Chiron R,Marchandin H

更新日期：2018-11-15 00:00:00

abstract：:Burkholderia pseudomallei is a saprophytic bacterium which is the causative agent of melioidosis, a common cause of fatal bacterial pneumonia and sepsis in the tropics. The incidence of melioidosis is clustered spatially and temporally and is heavily linked to rainfall and extreme weather events. Clinical case cluster...

journal_title：Applied and environmental microbiology

authors： Baker A,Tahani D,Gardiner C,Bristow KL,Greenhill AR,Warner J

更新日期：2011-10-01 00:00:00

abstract：:One emerging disease of grapevine in Europe is Bois noir (BN), a phytoplasmosis caused by "Candidatus Phytoplasma solani" and spread in vineyards by the planthopper Hyalesthes obsoletus (Hemiptera: Cixiidae). Here we present the first full characterization of the bacterial community of this important disease vector co...

journal_title：Applied and environmental microbiology

authors： Gonella E,Negri I,Marzorati M,Mandrioli M,Sacchi L,Pajoro M,Crotti E,Rizzi A,Clementi E,Tedeschi R,Bandi C,Alma A,Daffonchio D

更新日期：2011-02-01 00:00:00

abstract：:Geographical and seasonal variation in the incidence and prevalence of Campylobacter jejuni and C. coli in housed broiler flocks reared in Great Britain in 2004 to 2006 was investigated in this study. Ceca (30) from 797 flocks, not subject to prior partial depopulation and reared on 211 farms, were examined individual...

journal_title：Applied and environmental microbiology

authors： Jorgensen F,Ellis-Iversen J,Rushton S,Bull SA,Harris SA,Bryan SJ,Gonzalez A,Humphrey TJ

更新日期：2011-06-01 00:00:00

abstract：:3-Deoxy-D-arabino-heptulosonate 7-phosphate (DAHP) synthase (EC 2.5.1.54) catalyzes the first step of the shikimate pathway that finally leads to the biosynthesis of aromatic amino acids phenylalanine (Phe), tryptophan (Trp), and tyrosine (Tyr). In Corynebacterium glutamicum ATCC 13032, two chromosomal genes, NCgl0950...

journal_title：Applied and environmental microbiology

authors： Liu YJ,Li PP,Zhao KX,Wang BJ,Jiang CY,Drake HL,Liu SJ

更新日期：2008-09-01 00:00:00

abstract：:A heat-stable extracellular protease from Pseudomonas fluorescens was purified by chromatography on a DEAE-cellulose column and gel filtration on a Sephadex G100 column. The homogeneous enzyme preparation was used to prepare antiserum in rabbits. The rabbit antiserum was used to study the antigenic relatedness of prot...

journal_title：Applied and environmental microbiology

authors： Jackman DM,Bartlett FM,Patel TR

更新日期：1983-07-01 00:00:00

abstract：:Conjugal transfer of lactose-fermenting ability (Lac+), nisin resistance (Nisr), and phage resistance (Hsp+) was demonstrated in matings between Streptococcus lactis ME2 (donor) and Streptococcus cremoris M43a (recipient), a derivative of M12R. Transconjugants were detected by transfer of Lac+ and were found to exhibi...

journal_title：Applied and environmental microbiology

authors： Steenson LR,Klaenhammer TR

更新日期：1985-10-01 00:00:00

abstract：:An acetate-fermenting strain of Methanosarcina was isolated from an acetate enrichment culture inoculated with anaerobic sludge from a waste treatment digestor. In pure culture, this organism fermented acetate in the absence of added hydrogen at rates comparable in magnitude to those found in digestor systems. This ra...

journal_title：Applied and environmental microbiology

authors： Mah RA,Smith MR,Baresi L

更新日期：1978-06-01 00:00:00

abstract：:Conserved regions about 420 bp long of the pelADE cluster specific to Erwinia chrysanthemi were amplified by PCR and used to differentiate 78 strains of E. chrysanthemi that were obtained from different hosts and geographical areas. No PCR products were obtained from DNA samples extracted from other pectinolytic and n...

journal_title：Applied and environmental microbiology

authors： Nassar A,Darrasse A,Lemattre M,Kotoujansky A,Dervin C,Vedel R,Bertheau Y

更新日期：1996-07-01 00:00:00

abstract：:Gram-positive bacteria of the genus Exiguobacterium have been repeatedly isolated from Siberian permafrost ranging in age from 20,000 to 2 to 3 million years and have been sporadically recovered from markedly diverse habitats, including microbial mats in Lake Fryxell (Antarctic), surface water, and food-processing env...

journal_title：Applied and environmental microbiology

authors： Vishnivetskaya TA,Kathariou S

更新日期：2005-11-01 00:00:00

abstract：:The production of polyhydroxybutyrate (PHB) by Alcaligenes eutrophus DSM 545 was studied in a synthetic medium with 3% glucose at pH 7.0 supplemented with several ammonium substrates and cane molasses. Growth was measured by dry cell weight, and the PHB content was measured by gas chromatography. The effects of ammoni...

journal_title：Applied and environmental microbiology

authors： Beaulieu M,Beaulieu Y,Melinard J,Pandian S,Goulet J

更新日期：1995-01-01 00:00:00

abstract：:Enterococci are one of the major facultative anaerobic bacterial groups that reside in the human gastrointestinal tract. In the present study, the composition of the enterococcal fecal flora in three healthy humans was analyzed before, during, and after the daily consumption of approximately 125 g of a raw-milk Chedda...

journal_title：Applied and environmental microbiology

authors： Gelsomino R,Vancanneyt M,Cogan TM,Swings J

更新日期：2003-01-01 00:00:00

abstract：:Rhodococcus erythropolis I-19, containing multiple copies of key dsz genes, was used to desulfurize alkylated dibenzothiophenes (Cx-DBTs) found in a hydrodesulfurized middle-distillate petroleum (MD 1850). Initial desulfurization rates of dibenzothiophene (DBT) and MD 1850 by I-19 were 5.0 and 2.5 micromol g dry cell ...

journal_title：Applied and environmental microbiology

authors： Folsom BR,Schieche DR,DiGrazia PM,Werner J,Palmer S

更新日期：1999-11-01 00:00:00

abstract：:The intestinal contents of 59 Zairese freshwater fish were examined for the presence of potential human enteric pathogens. Edwardsiella tarda and Plesiomonas shigelloides were isolated from 57 and 59% of them, respectively. For both microorganisms there was a significant difference between the isolation rates from lak...

journal_title：Applied and environmental microbiology

authors： Van Damme LR,Vandepitte J

更新日期：1980-03-01 00:00:00

abstract：:We screened an Aspergillus tubingensis expression library constructed in the yeast Kluyveromyces lactis for xylogalacturonan-hydrolyzing activity in microwell plates by using a bicinchoninic acid assay. This assay detects reducing carbohydrate groups when they are released from a carbohydrate by enzymatic activity. Tw...

journal_title：Applied and environmental microbiology

authors： van der Vlugt-Bergmans CJ,Meeuwsen PJ,Voragen AG,van Ooyen AJ

更新日期：2000-01-01 00:00:00

abstract：:Flies may act as potential vectors for the spread of resistant bacteria to different environments. This study was intended to evaluate the presence of Escherichia coli strains resistant to cephalosporins in flies captured in the areas surrounding five broiler farms. Phenotypic and molecular characterization of the res...

journal_title：Applied and environmental microbiology

authors： Solà-Ginés M,González-López JJ,Cameron-Veas K,Piedra-Carrasco N,Cerdà-Cuéllar M,Migura-Garcia L

更新日期：2015-06-01 00:00:00

abstract：:This study presents population analyses of microbial communities inhabiting a site of extreme acid mine drainage (AMD) production. The site is the inactive underground Richmond mine at Iron Mountain, Calif., where the weathering of a massive sulfide ore body (mostly pyrite) produces solutions with pHs of approximately...

journal_title：Applied and environmental microbiology

authors： Bond PL,Druschel GK,Banfield JF

更新日期：2000-11-01 00:00:00