Localization of inulinase and invertase in Kluyveromyces species.

abstract：:Four different green fluorescent protein (GFP)-based whole-cell biosensors were created based on the DNA damage inducible SOS response of Escherichia coli in order to evaluate the sensitivity of individual SOS promoters toward genotoxic substances. Treatment with the known carcinogen N-methyl-N'-nitro-N-nitrosoguanidi...

journal_title：Applied and environmental microbiology

authors： Norman A,Hestbjerg Hansen L,Sørensen SJ

更新日期：2005-05-01 00:00:00

abstract：:The bacterial succession on the skin of broiler chicken carcasses stored at 2 degrees C was traced, and the ability of representative isolates to produce off-odors was determined by using sterile leg and breast muscle sections. Off-odors were identified by olfactory and chemical means. The inability of peptone-iron ag...

journal_title：Applied and environmental microbiology

authors： Daud HB,McMeekin TA,Thomas CJ

更新日期：1979-03-01 00:00:00

abstract：:A field study was conducted to determine the microbial community structures of streambed sediments across diverse geographic and climatic areas. Sediment samples were collected from three adjacent headwater forest streams within three biomes, eastern deciduous (Pennsylvania), southeastern coniferous (New Jersey), and ...

journal_title：Applied and environmental microbiology

authors： Findlay RH,Yeates C,Hullar MA,Stahl DA,Kaplan LA

更新日期：2008-05-01 00:00:00

abstract：:Ticks are important vectors for many emerging pathogens. However, they are also infected with many symbionts and commensals, often competing for the same niches. In this paper, we characterize the microbiome of Amblyomma americanum (Acari: Ixodidae), the lone star tick, in order to better understand the evolutionary r...

journal_title：Applied and environmental microbiology

authors： Ponnusamy L,Gonzalez A,Van Treuren W,Weiss S,Parobek CM,Juliano JJ,Knight R,Roe RM,Apperson CS,Meshnick SR

更新日期：2014-01-01 00:00:00

abstract：:Ari1p from Saccharomyces cerevisiae, recently identified as an intermediate-subclass short-chain dehydrogenase/reductase, contributes in situ to the detoxification of furfural. Furfural inhibits efficient ethanol production by yeast, particularly when the carbon source is acid-treated lignocellulose, which contains fu...

journal_title：Applied and environmental microbiology

authors： Bowman MJ,Jordan DB,Vermillion KE,Braker JD,Moon J,Liu ZL

更新日期：2010-08-01 00:00:00

abstract：:The preformed antimicrobial compounds produced by maize, 2,4-dihydroxy-7-methoxy-2H-1,4-benzoxazin-3-one and its desmethoxy derivative 2,4-dihydroxy-2H-1,4-benzoxazin-3-one, are highly reactive benzoxazinoids that quickly degrade to the antimicrobials 6-methoxy-2-benzoxazolinone (MBOA) and 2-benzoxazolinone (BOA), res...

journal_title：Applied and environmental microbiology

authors： Glenn AE,Hinton DM,Yates IE,Bacon CW

更新日期：2001-07-01 00:00:00

abstract：:Values of Delta(34)S (=delta(34)S(HS)-delta(34)S(SO(4)), where delta(34)S(HS) and delta(34)S(SO(4)) indicate the differences in the isotopic compositions of the HS(-) and SO(4)(2-) in the eluent, respectively) for many modern marine sediments are in the range of -55 to -75 per thousand, much greater than the -2 to -46...

journal_title：Applied and environmental microbiology

authors： Davidson MM,Bisher ME,Pratt LM,Fong J,Southam G,Pfiffner SM,Reches Z,Onstott TC

更新日期：2009-09-01 00:00:00

abstract：:The proportion of oocysts of Cryptosporidium parvum showing a fold on oocyst walls when incubated with either fluorescent monoclonal antibody or a surface-reactive fluorescent dye was increased by incubating suspensions of oocysts with dimethyl sulfoxide, sucrose, or Hanks' balanced salt solution. Further incubation o...

journal_title：Applied and environmental microbiology

authors： Robertson LJ,Campbell AT,Smith HV

更新日期：1993-08-01 00:00:00

abstract：:Eight strains of the entomopathogen Bacillus popilliae were analyzed by pulsed-field gel electrophoresis, and genomic size estimates of ;sim2,600 to 3,500 kb were obtained. The type strain, ATCC 14706, had a genomic size of 3,395 kb. For the six New Zealand isolates, the degree of similarity in the pulsed-field gel el...

journal_title：Applied and environmental microbiology

authors： Macdonald R,Kalmakoff J

更新日期：1995-06-01 00:00:00

abstract：:PCR generated two distinct products from a toxic isolate of Alexandrium catenella, which had been taken from Dai Ya Bay (southern China), by using primers for large-subunit rRNA. This pattern is distinct from published data for North American Alexandrium species. Sequences of the two products suggest that the smaller ...

journal_title：Applied and environmental microbiology

authors： Yeung PK,Kong KF,Wong FT,Wong JT

更新日期：1996-11-01 00:00:00

abstract：:Staphylococcal enterotoxins (SE) can cause toxin-mediated disease, and those that function as superantigens are implicated in the pathogenesis of allergic diseases. The prevalence of 19 enterotoxin genes was determined by PCR in clinical S. aureus strains derived from wounds (108) and blood (99). We performed spa typi...

journal_title：Applied and environmental microbiology

authors： Varshney AK,Mediavilla JR,Robiou N,Guh A,Wang X,Gialanella P,Levi MH,Kreiswirth BN,Fries BC

更新日期：2009-11-01 00:00:00

abstract：:The phylogenetic diversity and species richness of ammonia-oxidizing archaea (AOA) and bacteria (AOB) were examined with aquarium biofiltration systems. Species richness, deduced from rarefaction analysis, and diversity indices indicated that the phylogenetic diversity and species richness of AOA are greater than thos...

journal_title：Applied and environmental microbiology

authors： Urakawa H,Tajima Y,Numata Y,Tsuneda S

更新日期：2008-02-01 00:00:00

abstract：:The effects of environmental stress exposure on Listeria monocytogenes growth and virulence-associated characteristics were investigated. Specifically, we measured the effects of temperature (7 or 37 degrees C), pH (5.5 or 7.4), the presence of salt and organic acids (375 mM NaCl, 8.45 mM sodium diacetate [SD], 275 mM...

journal_title：Applied and environmental microbiology

authors： Garner MR,James KE,Callahan MC,Wiedmann M,Boor KJ

更新日期：2006-08-01 00:00:00

abstract：:The methylotrophic yeast Pichia pastoris is widely used for the expression of heterologous enzymes. While the purity of the desired expression product is of major importance for many applications, we found that recombinant enzymes produced in methanol medium were contaminated by a 37-kDa endogenous yeast protease. Thi...

journal_title：Applied and environmental microbiology

authors： Salamin K,Sriranganadane D,Léchenne B,Jousson O,Monod M

更新日期：2010-07-01 00:00:00

abstract：:Through use of commercially available DnaK proteins and anti-DnaK monoclonal antibodies, a competitive enzyme-linked immunosorbent assay was developed to quantify this heat shock protein in Escherichia coli ATCC 25922 subjected to various heating regimens. For a given process lethality (F(70)(10) of 1, 3, and 5 min), ...

journal_title：Applied and environmental microbiology

authors： Seyer K,Lessard M,Piette G,Lacroix M,Saucier L

更新日期：2003-06-01 00:00:00

abstract：:We examined the genetic structure and symbiotic characteristics of Bradyrhizobium isolates recovered from four legume species (Lupinus albus [white lupine], Lupinus angustifolius [blue lupine], Ornithopus compressus [yellow serradella], and Macroptilium atropurpureum [sirato]) grown in an Oregon soil. We established t...

journal_title：Applied and environmental microbiology

authors： Bottomley PJ,Cheng HH,Strain SR

更新日期：1994-06-01 00:00:00

abstract：:Green mold disease (causal agent, Trichoderma) has resulted in severe crop losses on mushroom farms worldwide in recent years. We analyzed 160 isolates of Trichoderma from mushroom farms for morphological, cultural, and molecular characteristics and classified these isolates into phenotypic groups. The most common gro...

journal_title：Applied and environmental microbiology

authors： Castle A,Speranzini D,Rghei N,Alm G,Rinker D,Bissett J

更新日期：1998-01-01 00:00:00

abstract：:Environmental bacteria persist in various habitats, yet little is known about the genes that contribute to growth and survival in their respective ecological niches. Signature-tagged mutagenesis (STM) of Shewanella oneidensis MR-1 coupled with a screen involving incubations of mutant strains in anoxic aquifer sediment...

journal_title：Applied and environmental microbiology

authors： Groh JL,Luo Q,Ballard JD,Krumholz LR

更新日期：2007-01-01 00:00:00

abstract：:Fusarium species from agricultural crops have been well studied with respect to toxin production and genetic diversity, while similar studies of communities from nonagricultural plants are much more limited. We examined 72 Fusarium isolates from a native North American tallgrass prairie and found that Gibberella inter...

journal_title：Applied and environmental microbiology

authors： Leslie JF,Zeller KA,Logrieco A,Mulè G,Moretti A,Ritieni A

更新日期：2004-04-01 00:00:00

abstract：:We describe here the development of a single-reaction multiplex PCR assay for the enterotoxin genes from Staphylococcus aureus that utilizes a universal toxin gene primer in combination with toxin-specific primers to amplify characteristic toxin gene products. In combination with a new DNA purification method, the ass...

journal_title：Applied and environmental microbiology

authors： Sharma NK,Rees CE,Dodd CE

更新日期：2000-04-01 00:00:00

abstract：:Hyphomicrobium chloromethanicum CM2(T), an aerobic methylotrophic member of the alpha subclass of the class proteobacteria, can grow with chloromethane as the sole carbon and energy source. H. chloromethanicum possesses an inducible enzyme system for utilization of chloromethane, in which two polypeptides (67-kDa CmuA...

journal_title：Applied and environmental microbiology

authors： McAnulla C,Woodall CA,McDonald IR,Studer A,Vuilleumier S,Leisinger T,Murrell JC

更新日期：2001-01-01 00:00:00

abstract：:We investigated previously unknown associations between bacterial endosymbionts and bat flies of the subfamily Nycterophiliinae (Diptera, Streblidae). Molecular analyses revealed a novel clade of Gammaproteobacteria in Nycterophilia bat flies. This clade was not closely related to Arsenophonus-like microbes found in i...

journal_title：Applied and environmental microbiology

authors： Morse SF,Dick CW,Patterson BD,Dittmar K

更新日期：2012-12-01 00:00:00

abstract：:A soy sauce koji mold, Aspergillus flavus var. columnaris Raper and Fennel (ATCC 44310), was treated with UV irradiation to obtain mutant strains possessing high protease activities, high amylase activities, and light-colored conidia. Selected mutant strains were tested for toxicity, and some were found acutely toxic ...

journal_title：Applied and environmental microbiology

authors： Kalayanamitr A,Bhumiratana A,Flegel TW,Glinsukon T,Shinmyo A

更新日期：1987-08-01 00:00:00

abstract：:Respiration rates of bacterial cultures can be a powerful tool in gauging the effects of genetic manipulation and environmental changes affecting overall metabolism. We present an optical method for measuring respiration rates using a robust phosphorescence lifetime-based sensor and off-the-shelf technology. This meth...

journal_title：Applied and environmental microbiology

authors： Strovas TJ,Dragavon JM,Hankins TJ,Callis JB,Burgess LW,Lidstrom ME

更新日期：2006-02-01 00:00:00

abstract：:We report here the cloning and sequencing of the gene for proline dehydrogenase (putA) of Bradyrhizobium japonicum. An open reading frame coding for 1,016 amino acids was identified. The B. japonicum gene codes for a bifunctional protein with proline dehydrogenase and pyrroline-5-carboxylate (P5C) dehydrogenase activi...

journal_title：Applied and environmental microbiology

authors： Straub PF,Reynolds PH,Althomsons S,Mett V,Zhu Y,Shearer G,Kohl DH

更新日期：1996-01-01 00:00:00

abstract：:The insertion of a heterologous gene into commensal bacteria is a common technique to develop a delivery agent for vaccination and therapies, but the pleiotropic effects of genetic modifications need to be investigated before its use in practical applications. Although supplemental properties provided by the expressio...

journal_title：Applied and environmental microbiology

authors： Kajikawa A,Igimi S

更新日期：2009-05-01 00:00:00

abstract：:Salmonella enterica contamination in foods is a significant concern for public health. When DNA detection methods are used for analysis of foods, one of the major concerns is false-positive results from the detection of dead cells. To circumvent this crucial issue, a TaqMan quantitative real-time RT-PCR (qRT-PCR) assa...

journal_title：Applied and environmental microbiology

authors： González-Escalona N,Hammack TS,Russell M,Jacobson AP,De Jesús AJ,Brown EW,Lampel KA

更新日期：2009-06-01 00:00:00

abstract：:DNA was extracted from dry standing dead Spartina alterniflora stalks as well as dry Spartina wrack from the North Inlet (South Carolina) and Sapelo Island (Georgia) salt marshes. Partial nifH sequences were PCR amplified, the products were separated by denaturing gradient gel electrophoresis (DGGE), and the prominent...

journal_title：Applied and environmental microbiology

authors： Lovell CR,Friez MJ,Longshore JW,Bagwell CE

更新日期：2001-11-01 00:00:00

abstract：:The effects of mineral fertilizer (NPK) and organic manure on the community structure of soil ammonia-oxidizing bacteria (AOB) was investigated in a long-term (16-year) fertilizer experiment. The experiment included seven treatments: organic manure, half organic manure N plus half fertilizer N, fertilizer NPK, fertili...

journal_title：Applied and environmental microbiology

authors： Chu H,Fujii T,Morimoto S,Lin X,Yagi K,Hu J,Zhang J

更新日期：2007-01-01 00:00:00

abstract：:Disruption of the aflatoxin biosynthesis cluster gene aflY (hypA) gave Aspergillus parasiticus transformants that accumulated versicolorin A. This gene is predicted to encode the Baeyer-Villiger oxidase necessary for formation of the xanthone ring of the aflatoxin precursor demethylsterigmatocystin. ...

journal_title：Applied and environmental microbiology

authors： Ehrlich KC,Montalbano B,Boué SM,Bhatnagar D

更新日期：2005-12-01 00:00:00