Abstract:
:DNA was extracted from dry standing dead Spartina alterniflora stalks as well as dry Spartina wrack from the North Inlet (South Carolina) and Sapelo Island (Georgia) salt marshes. Partial nifH sequences were PCR amplified, the products were separated by denaturing gradient gel electrophoresis (DGGE), and the prominent DGGE bands were sequenced. Most sequences (109 of 121) clustered with those from alpha-Proteobacteria, and 4 were very similar (>99%) to that of Azospirillum brasilense. Seven sequences clustered with those from known gamma-Proteobacteria and five with those from known anaerobic diazotrophs. The diazotroph assemblages associated with dead Spartina biomass in these two salt marshes were very similar, and relatively few major lineages were represented.
journal_name
Appl Environ Microbioljournal_title
Applied and environmental microbiologyauthors
Lovell CR,Friez MJ,Longshore JW,Bagwell CEdoi
10.1128/AEM.67.11.5308-5314.2001keywords:
subject
Has Abstractpub_date
2001-11-01 00:00:00pages
5308-14issue
11eissn
0099-2240issn
1098-5336journal_volume
67pub_type
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