Recovery and phylogenetic analysis of nifH sequences from diazotrophic bacteria associated with dead aboveground biomass of Spartina alterniflora.

Abstract:

:DNA was extracted from dry standing dead Spartina alterniflora stalks as well as dry Spartina wrack from the North Inlet (South Carolina) and Sapelo Island (Georgia) salt marshes. Partial nifH sequences were PCR amplified, the products were separated by denaturing gradient gel electrophoresis (DGGE), and the prominent DGGE bands were sequenced. Most sequences (109 of 121) clustered with those from alpha-Proteobacteria, and 4 were very similar (>99%) to that of Azospirillum brasilense. Seven sequences clustered with those from known gamma-Proteobacteria and five with those from known anaerobic diazotrophs. The diazotroph assemblages associated with dead Spartina biomass in these two salt marshes were very similar, and relatively few major lineages were represented.

journal_name

Appl Environ Microbiol

authors

Lovell CR,Friez MJ,Longshore JW,Bagwell CE

doi

10.1128/AEM.67.11.5308-5314.2001

keywords:

subject

Has Abstract

pub_date

2001-11-01 00:00:00

pages

5308-14

issue

11

eissn

0099-2240

issn

1098-5336

journal_volume

67

pub_type

杂志文章
  • Identification of Bifidobacterium strains by rRNA gene restriction patterns.

    abstract::Total DNA from 21 collection or industrial Bifidobacterium strains was cleaved with various restriction endonucleases. Following electrophoresis, the fragments were subjected to Southern blot hybridization with a heterologous [alpha-32P]dCTP-labeled rDNA (genes coding for rRNA) 23S gene probe. The ribosomal patterns a...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.60.5.1451-1458.1994

    authors: Mangin I,Bourget N,Bouhnik Y,Bisetti N,Simonet JM,Decaris B

    更新日期:1994-05-01 00:00:00

  • Glutathione protects Lactococcus lactis against oxidative stress.

    abstract::Glutathione was found in several dairy Lactococcus lactis strains grown in M17 medium. None of these strains was able to synthesize glutathione. In chemically defined medium, L. lactis subsp. cremoris strain SK11 was able to accumulate up to approximately 60 mM glutathione when this compound was added to the medium. S...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/aem.69.10.5739-5745.2003

    authors: Li Y,Hugenholtz J,Abee T,Molenaar D

    更新日期:2003-10-01 00:00:00

  • Microbiological comparison of surface soil and unsaturated subsurface soil from a semiarid high desert.

    abstract::Thirty-two chemoheterotrophic bacteria were isolated from unsaturated subsurface soil samples obtained from ca. 70 m below land surface in a high desert in southeastern Idaho. Most isolates were gram positive (84%) and strict aerobes (79%). Acridine orange direct counts of microbes in one subsurface sample showed lowe...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.55.9.2420-2423.1989

    authors: Colwell FS

    更新日期:1989-09-01 00:00:00

  • Antifungal metabolites (monorden, monocillin IV, and cerebrosides) from Humicola fuscoatra traaen NRRL 22980, a mycoparasite of Aspergillus flavus sclerotia.

    abstract::The mycoparasite Humicola fuscoatra NRRL 22980 was isolated from a sclerotium of Aspergillus flavus that had been buried in a cornfield near Tifton, Ga. When grown on autoclaved rice, this fungus produced the antifungal metabolites monorden, monocillin IV, and a new monorden analog. Each metabolite produced a clear zo...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.64.11.4482-4484.1998

    authors: Wicklow DT,Joshi BK,Gamble WR,Gloer JB,Dowd PF

    更新日期:1998-11-01 00:00:00

  • Shiga toxins, and the genes encoding them, in fecal samples from native Idaho ungulates.

    abstract::Cattle are a known reservoir of Shiga toxin-producing Escherichia coli. The prevalence and stability of Shiga toxin and/or Shiga toxin genes among native wild ungulates in Idaho were investigated. The frequency of both Shiga genes and toxin was similar to that reported for Idaho cattle ( approximately 19%). ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.01158-08

    authors: Gilbreath JJ,Shields MS,Smith RL,Farrell LD,Sheridan PP,Spiegel KM

    更新日期:2009-02-01 00:00:00

  • Effects of organic substrates on dechlorination of aroclor 1242 in anaerobic sediments.

    abstract::The effects of different organic substrates on the abilities of anaerobic sediment enrichments to reductively dechlorinate polychlorinated biphenyls (PCBs) were studied. Sediments collected from a site previously contaminated with PCBs were dosed with additional PCBs (Aroclor 1242; approximately 300 ppm [300 mug/g], s...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.56.9.2612-2617.1990

    authors: Nies L,Vogel TM

    更新日期:1990-09-01 00:00:00

  • Occurrence and phenotypic characterization of Yersinia ruckeri strains with biofilm-forming capacity in a rainbow trout farm.

    abstract::The presence of Yersinia ruckeri in a French fish farm was investigated. Y. ruckeri was isolated mainly from algae and sediment samples rather than from water. Twenty-two Y. ruckeri isolates were obtained, and three strains were distinguished by enterobacterial repetitive intergenic consensus PCR amplification. These ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/aem.68.2.470-475.2002

    authors: Coquet L,Cosette P,Quillet L,Petit F,Junter GA,Jouenne T

    更新日期:2002-02-01 00:00:00

  • Taking the starch out of oral biofilm formation: molecular basis and functional significance of salivary α-amylase binding to oral streptococci.

    abstract::α-Amylase-binding streptococci (ABS) are a heterogeneous group of commensal oral bacterial species that comprise a significant proportion of dental plaque microfloras. Salivary α-amylase, one of the most abundant proteins in human saliva, binds to the surface of these bacteria via specific surface-exposed α-amylase-bi...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章,评审

    doi:10.1128/AEM.02581-12

    authors: Nikitkova AE,Haase EM,Scannapieco FA

    更新日期:2013-01-01 00:00:00

  • Localization and visualization of a coxiella-type symbiont within the lone star tick, Amblyomma americanum.

    abstract::A Coxiella-type microbe occurs at 100% frequency in all Amblyomma americanum ticks thus far tested. Using laboratory-reared ticks free of other microbes, we identified the Amblyomma-associated Coxiella microbe in several types of tissue and at various stages of the life cycle of A. americanum by 16S rRNA gene sequenci...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.00537-07

    authors: Klyachko O,Stein BD,Grindle N,Clay K,Fuqua C

    更新日期:2007-10-01 00:00:00

  • Nitrogen availability of grape juice limits killer yeast growth and fermentation activity during mixed-culture fermentation with sensitive commercial yeast strains.

    abstract::The competition between selected or commercial killer strains of type K2 and sensitive commercial strains of Saccharomyces cerevisiae was studied under various conditions in sterile grape juice fermentations. The focus of this study was the effect of yeast inoculation levels and the role of assimilable nitrogen nutrit...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.63.7.2821-2825.1997

    authors: Medina K,Carrau FM,Gioia O,Bracesco N

    更新日期:1997-07-01 00:00:00

  • Influence of the membrane on T-2 toxin toxicity in Saccharomyces spp.

    abstract::In growing cells of Saccharomyces cerevisiae and Saccharomyces carlsbergensis, T-2 toxin inhibits cell growth. We have examined the role of the yeast membranes in the uptake mechanism(s) of T-2 toxin. The effects of membrane-modulating agents, ethanol, cetyltrimethylammonium bromide, Triton X-100, and heat were studie...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.47.4.681-684.1984

    authors: Schappert KT,Khachatourians GG

    更新日期:1984-04-01 00:00:00

  • Criteria and methodology for identifying respiratory denitrifiers.

    abstract::Respiratory denitrification is not always adequately established when bacteria are characterized. We have tested a simple method that allows one to evaluate whether the two necessary criteria to claim denitrification have been met, namely, that N(inf2) or N(inf2)O is produced from nitrate or nitrite and that this redu...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.61.3.1110-1115.1995

    authors: Mahne I,Tiedje JM

    更新日期:1995-03-01 00:00:00

  • Binding of Insecticidal Crystal Proteins of Bacillus thuringiensis to the Midgut Brush Border of the Cabbage Looper, Trichoplusia ni (Hübner) (Lepidoptera: Noctuidae), and Selection for Resistance to One of the Crystal Proteins.

    abstract::The susceptibility of Trichoplusia ni larvae to several Bacillus thuringiensis insecticidal crystal proteins (ICPs) was tested. Neonatal larvae proved to be susceptible to solubilized trypsin-treated CryIA(a), CryIA(b), and CryIA(c) (50% lethal concentrations [LC(50)s], 570, 480, and 320 ng/cm, respectively) but showe...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.60.10.3840-3846.1994

    authors: Estada U,Ferre J

    更新日期:1994-10-01 00:00:00

  • Oxidation of Nitrapyrin to 6-Chloropicolinic Acid by the Ammonia-Oxidizing Bacterium Nitrosomonas europaea.

    abstract::Suspensions of Nitrosomonas europaea catalyzed the oxidation of the commercial nitrification inhibitor nitrapyrin [2-chloro-6-(trichloromethyl)-pyridine]. Rapid oxidation of nitrapyrin (at a concentration of 10 muM) required the concomitant oxidation of ammonia, hydroxylamine, or hydrazine. The turnover rate was highe...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.58.7.2321-2325.1992

    authors: Vannelli T,Hooper AB

    更新日期:1992-07-01 00:00:00

  • Oxygen-sensing reporter strain of Pseudomonas fluorescens for monitoring the distribution of low-oxygen habitats in soil.

    abstract::The root-colonizing bacterium Pseudomonas fluorescens CHA0 was used to construct an oxygen-responsive biosensor. An anaerobically inducible promoter of Pseudomonas aeruginosa, which depends on the FNR (fumarate and nitrate reductase regulation)-like transcriptional regulator ANR (anaerobic regulation of arginine deimi...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.65.9.4085-4093.1999

    authors: Højberg O,Schnider U,Winteler HV,Sørensen J,Haas D

    更新日期:1999-09-01 00:00:00

  • Use of a marker organism to model the spread of central nervous system tissue in cattle and the abattoir environment during commercial stunning and carcass dressing.

    abstract::Due to concerns about a link between variant Creutzfeldt-Jakob disease in humans and similar prion protein-induced disease in cattle, i.e., bovine spongiform encephalopathy (BSE), strict controls are in place to exclude BSE-positive animals and/or specified risk materials including bovine central nervous system (CNS) ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/aem.68.2.791-798.2002

    authors: Daly DJ,Prendergast DM,Sheridan JJ,Blair IS,McDowell DA

    更新日期:2002-02-01 00:00:00

  • Viability of lyophilized cyanobacteria (blue-green algae).

    abstract::Lyophilizatin of 13 cyanobacterial cultures belonging to seven genera was attempted in a variety of suspending substances. All organisms survived lyophilization when suspended in lamb serum. Some of the organisms could be successfully lyophilized in horse serum, beef serum, fetal bovine serum, or human ascites fluid. ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.32.6.777-780.1976

    authors: Corbett LL,Parker DL

    更新日期:1976-12-01 00:00:00

  • Characterization of aquatic bacteria and cloning of genes specifying partial degradation of 2,4-dichlorophenoxyacetic acid.

    abstract::Water samples from rivers, streams, ponds, and activated sewage were tested for the presence of bacteria which utilize 2,4-dichlorophenoxyacetic acid (2,4-D) as a sole source of carbon. Seventy percent of the attempted enrichments yielded pure cultures of 2,4-D-metabolizing bacteria. All but 1 of the 30 isolates were ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.49.5.1237-1245.1985

    authors: Amy PS,Schulke JW,Frazier LM,Seidler RJ

    更新日期:1985-05-01 00:00:00

  • Chemical transformation of eremofortin C into PR toxin.

    abstract::The natural products of both eremofortin C (EC) and PR toxin are secondary metabolites of Penicillium roqueforti. Because the chemical structures of EC and PR toxin are closely related to each other and differ only by a hydroxyl functional group in EC and an aldehyde functional group in PR toxin at the C-12 position, ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.50.3.729-731.1985

    authors: Li SY,Chang SC,Wei RD

    更新日期:1985-09-01 00:00:00

  • Use of pulsed-field gel electrophoresis to link sporadic cases of invasive listeriosis with recalled chocolate milk.

    abstract::Pulsed-field gel electrophoresis established the linkage between recalled chocolate milk and a multistate invasive listeriosis outbreak during a four-product recall period. Listeria monocytogenes isolates from four hospitalized patients and an environmental dairy sample displayed AscI restriction endonuclease digestio...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.61.8.3177-3179.1995

    authors: Proctor ME,Brosch R,Mellen JW,Garrett LA,Kaspar CW,Luchansky JB

    更新日期:1995-08-01 00:00:00

  • Induced Natural Transformation of Acinetobacter calcoaceticus in Soil Microcosms.

    abstract::Factors affecting natural transformation of Acinetobacter calcoaceticus BD413 with homologous chromosomal DNA in a silt loam soil microcosm were investigated. Inducible transformation of declining populations of noncompetent A. calcoaceticus cells was detectable for up to 6 days when a simple carbon source, salts, and...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.63.10.3972-3977.1997

    authors: Nielsen KM,Bones AM,Van Elsas JD

    更新日期:1997-10-01 00:00:00

  • Presence and characterization of shiga toxin-producing Escherichia coli and other potentially diarrheagenic E. coli strains in retail meats.

    abstract::To determine the presence of Shiga toxin-producing Escherichia coli (STEC) and other potentially diarrheagenic E. coli strains in retail meats, 7,258 E. coli isolates collected by the U.S. National Antimicrobial Resistance Monitoring System (NARMS) retail meat program from 2002 to 2007 were screened for Shiga toxin ge...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.01968-09

    authors: Xia X,Meng J,McDermott PF,Ayers S,Blickenstaff K,Tran TT,Abbott J,Zheng J,Zhao S

    更新日期:2010-03-01 00:00:00

  • Streptococcus suis serotypes characterized by analysis of chaperonin 60 gene sequences.

    abstract::Streptococcus suis is an important pathogen of swine which occasionally infects humans as well. There are 35 serotypes known for this organism, and it would be desirable to develop rapid methods methods to identify and differentiate the strains of this species. To that effect, partial chaperonin 60 gene sequences were...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/aem.67.10.4828-4833.2001

    authors: Brousseau R,Hill JE,Préfontaine G,Goh SH,Harel J,Hemmingsen SM

    更新日期:2001-10-01 00:00:00

  • Susceptibility of halobacteria to heavy metals.

    abstract::Sixty-eight halobacteria, including both culture collection strains and fresh isolates from widely differing geographical areas, were tested for susceptibility to arsenate, cadmium, chromium, cobalt, copper, lead, mercury, nickel, silver, and zinc ions by an agar dilution technique. The culture collection strains show...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.53.5.1199-1202.1987

    authors: Nieto JJ,Ventosa A,Ruiz-Berraquero F

    更新日期:1987-05-01 00:00:00

  • The twin-arginine signal peptide of Bacillus subtilis YwbN can direct either Tat- or Sec-dependent secretion of different cargo proteins: secretion of active subtilisin via the B. subtilis Tat pathway.

    abstract::Proteins that are produced for commercial purposes in Bacillus subtilis are commonly secreted via the Sec pathway. Despite its high secretion capacity, the secretion of heterologous proteins via the Sec pathway is often unsuccessful. Alternative secretion routes, like the Tat pathway, are therefore of interest. Two pa...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.01401-08

    authors: Kolkman MA,van der Ploeg R,Bertels M,van Dijk M,van der Laan J,van Dijl JM,Ferrari E

    更新日期:2008-12-01 00:00:00

  • Toxic and enzymatic activities of Vibrio vulnificus biotype 2 with respect to host specificity.

    abstract::In this work, the enzymatic activities of selected strains of biotypes 1 and 2 of Vabrio vulnificus were analyzed by using conventional methods and the API ZYM system. The toxic activities of extracellular products (ECPs) were further evaluated by in vitro and in vivo experiments. The ECPs of both biotypes (i) showed ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.62.7.2331-2337.1996

    authors: Biosca EG,Amaro C

    更新日期:1996-07-01 00:00:00

  • Species co-occurrence patterns among Lyme borreliosis pathogens in the tick vector Ixodes ricinus.

    abstract::Mixed infections have important consequences for the ecology and evolution of host-parasite interactions. In vector-borne diseases, interactions between pathogens occur in both the vertebrate host and the arthropod vector. Spirochete bacteria belonging to the Borrelia burgdorferi sensu lato genospecies complex are tra...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.02158-13

    authors: Herrmann C,Gern L,Voordouw MJ

    更新日期:2013-12-01 00:00:00

  • Genetic and Phenotypic Diversity of Bacillus polymyxa in Soil and in the Wheat Rhizosphere.

    abstract::Diversity among 130 strains of Bacillus polymyxa was studied; the bacteria were isolated by immunotrapping from nonrhizosphere soil (32 strains), rhizosphere soil (38 strains), and the rhizoplane (60 strains) of wheat plantlets growing in a growth chamber. The strains were characterized phenotypically by 63 auxanograp...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.58.6.1894-1903.1992

    authors: Mavingui P,Laguerre G,Berge O,Heulin T

    更新日期:1992-06-01 00:00:00

  • Degradation of Bis(4-Hydroxyphenyl)methane (bisphenol F) by Sphingobium yanoikuyae strain FM-2 isolated from river water.

    abstract::Three bacteria capable of utilizing bis(4-hydroxyphenyl)methane (bisphenol F [BPF]) as the sole carbon source were isolated from river water, and they all belonged to the family Sphingomonadaceae. One of the isolates, designated Sphingobium yanoikuyae strain FM-2, at an initial cell density of 0.01 (optical density at...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.01708-07

    authors: Inoue D,Hara S,Kashihara M,Murai Y,Danzl E,Sei K,Tsunoi S,Fujita M,Ike M

    更新日期:2008-01-01 00:00:00

  • Composition of Bacterial Communities Associated with Aurelia aurita Changes with Compartment, Life Stage, and Population.

    abstract::The scyphozoan Aurelia aurita is recognized as a key player in marine ecosystems and a driver of ecosystem change. It is thus intensely studied to address ecological questions, although its associations with microorganisms remain so far undescribed. In the present study, the microbiota associated with A. aurita was vi...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.01601-15

    authors: Weiland-Bräuer N,Neulinger SC,Pinnow N,Künzel S,Baines JF,Schmitz RA

    更新日期:2015-09-01 00:00:00