Localization and visualization of a coxiella-type symbiont within the lone star tick, Amblyomma americanum.

Abstract:

:A Coxiella-type microbe occurs at 100% frequency in all Amblyomma americanum ticks thus far tested. Using laboratory-reared ticks free of other microbes, we identified the Amblyomma-associated Coxiella microbe in several types of tissue and at various stages of the life cycle of A. americanum by 16S rRNA gene sequencing and diagnostic PCR. We visualized Amblyomma-associated Coxiella through the use of a diagnostic fluorescence in situ hybridization (FISH) assay supplemented with PCR-based detection, nucleic acid fluorescent staining, wide-field epifluorescence and confocal microscopy, and transmission electron microscopy (TEM). Specific fluorescent foci were observed in several tick tissues, including the midgut and the Malpighian tubules, but particularly bright signals were observed in the granular acini of salivary gland clusters and in both small and large oocytes. TEM confirmed intracellular bacterial structures in the same tissues. The presence of Amblyomma-associated Coxiella within oocytes is consistent with the vertical transmission of these endosymbionts. Further, the presence of the Amblyomma-associated Coxiella symbiont in other tissues such as salivary glands could potentially lead to interactions with horizontally acquired pathogens.

journal_name

Appl Environ Microbiol

authors

Klyachko O,Stein BD,Grindle N,Clay K,Fuqua C

doi

10.1128/AEM.00537-07

subject

Has Abstract

pub_date

2007-10-01 00:00:00

pages

6584-94

issue

20

eissn

0099-2240

issn

1098-5336

pii

AEM.00537-07

journal_volume

73

pub_type

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