Abstract:
:The composition of phospholipids from Mycobacterium convolutum R22 was determined after growth at two temperatures (20 and 30 degrees C) with 1-chlorohexadecane as the substrate. Comparisons were made with the phospholipids of cells grown on n-hexadecane. Phosphatidylinositolmannosides and phosphatidylethanolamine (PE) were the major phospholipids in n-hexadecane-grown cells. In 1-chlorohexadecane-grown cells, phosphatidylinositolmannosides were approximately half of the total phospholipids, with lesser amounts of PE and cardiolipin (CL). The relative level of PE was greater at 20 degrees C (versus that at 30 degrees C) after growth on either substrate. A determination was made of structure and positional distribution of constituent fatty acid in both CL and PE. The relative amount of unsaturated fatty acid was higher at 20 degrees C. There were two C16:1 fatty acids (C16:1 delta 9 and C16:1 delta 11), and these had positional preferences in both CL and PE. The positional sites of chlorinated fatty acids differed in both CL and PE at the two temperatures. The results confirm that microorganisms can specifically distribute chlorinated fatty acids into cellular phospholipids.
journal_name
Appl Environ Microbioljournal_title
Applied and environmental microbiologyauthors
Murphy GL,Perry JJdoi
10.1128/AEM.53.1.10-13.1987subject
Has Abstractpub_date
1987-01-01 00:00:00pages
10-3issue
1eissn
0099-2240issn
1098-5336journal_volume
53pub_type
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journal_title:Applied and environmental microbiology
pub_type: 杂志文章
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更新日期:1991-09-01 00:00:00
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