Distribution and survival of motile Aeromonas spp. in brackish water receiving sewage treatment effluent.

abstract：:Hydrolytic extracellular enzymes degrading host tissues potentially play a role in bacterial pathogenesis. Flavobacterium psychrophilum is an important bacterial pathogen of salmonid fish reared in freshwater throughout the world. Diversity among isolates has been described at the phenotypic, serological, and genomic ...

journal_title：Applied and environmental microbiology

authors： Rochat T,Pérez-Pascual D,Nilsen H,Carpentier M,Bridel S,Bernardet JF,Duchaud E

更新日期：2019-03-06 00:00:00

abstract：:Studies of time-dependent drug and environmental effects on single, live bacterial cells would benefit significantly from a permeable, nonperturbative, long-lived fluorescent stain specific to the nucleoids (chromosomal DNA). The ideal stain would not affect cell growth rate or nucleoid morphology and dynamics, even d...

journal_title：Applied and environmental microbiology

authors： Bakshi S,Choi H,Rangarajan N,Barns KJ,Bratton BP,Weisshaar JC

更新日期：2014-08-01 00:00:00

abstract：:Two anaerobic bacteria were isolated from polyethylene glycol (PEG)-degrading, methanogenic, enrichment cultures obtained from a municipal sludge digester. One isolate, identified as Desulfovibrio desulfuricans (strain DG2), metabolized oligomers ranging from ethylene glycol (EG) to tetraethylene glycol. The other iso...

journal_title：Applied and environmental microbiology

authors： Dwyer DF,Tiedje JM

更新日期：1986-10-01 00:00:00

abstract：:Rhizonin is a hepatotoxic cyclopeptide isolated from cultures of a fungal Rhizopus microsporus strain that grew on moldy ground nuts in Mozambique. Reinvestigation of this fungal strain by a series of experiments unequivocally revealed that this "first mycotoxin from lower fungi" is actually not produced by the fungus...

journal_title：Applied and environmental microbiology

authors： Partida-Martinez LP,de Looss CF,Ishida K,Ishida M,Roth M,Buder K,Hertweck C

更新日期：2007-02-01 00:00:00

abstract：:In this work, we report the expression and secretion of the leaderless two-peptide (EntL50A and EntL50B) bacteriocin enterocin L50 from Enterococcus faecium L50 by the methylotrophic yeast Pichia pastoris X-33. The bacteriocin structural genes entL50A and entL50B were fused to the Saccharomyces cerevisiae gene region ...

journal_title：Applied and environmental microbiology

authors： Basanta A,Gómez-Sala B,Sánchez J,Diep DB,Herranz C,Hernández PE,Cintas LM

更新日期：2010-05-01 00:00:00

abstract：:We describe a novel quantitative real-time (Q)-PCR assay for Listeria monocytogenes based on the coamplification of a target hly gene fragment and an internal amplification control (IAC). The IAC is a chimeric double-stranded DNA containing a fragment of the rapeseed BnACCg8 gene flanked by the hly-specific target seq...

journal_title：Applied and environmental microbiology

authors： Rodríguez-Lázaro D,Pla M,Scortti M,Monzó HJ,Vázquez-Boland JA

更新日期：2005-12-01 00:00:00

abstract：:The killing of Listeria monocytogenes following exposure to low pH, organic acids, and osmotic stress was enhanced by the addition of 5% (vol/vol) ethanol. At pH 3, for example, the presence of this agent stimulated killing by more than 3 log units in 40 min of exposure. The rate of cell death at pH 3.0 was dependent ...

journal_title：Applied and environmental microbiology

authors： Barker C,Park SF

更新日期：2001-04-01 00:00:00

abstract：:It has been suggested (L. H. Harris, R. P. Desai, N. E. Welker, and E. T. Papoutsakis, Biotechnol. Bioeng. 67:1-11, 2000) that butyryl phosphate (BuP) is a regulator of solventogenesis in Clostridium acetobutylicum. Here, we determined BuP and acetyl phosphate (AcP) levels in fermentations of C. acetobutylicum wild ty...

journal_title：Applied and environmental microbiology

authors： Zhao Y,Tomas CA,Rudolph FB,Papoutsakis ET,Bennett GN

更新日期：2005-01-01 00:00:00

abstract：:Sixty-eight halobacteria, including both culture collection strains and fresh isolates from widely differing geographical areas, were tested for susceptibility to arsenate, cadmium, chromium, cobalt, copper, lead, mercury, nickel, silver, and zinc ions by an agar dilution technique. The culture collection strains show...

journal_title：Applied and environmental microbiology

authors： Nieto JJ,Ventosa A,Ruiz-Berraquero F

更新日期：1987-05-01 00:00:00

abstract：:The susceptibility of a strain of Legionella pneumophila to disinfection by an organic halamine, free chlorine, and a mixture of the organic halamine and free chlorine was assessed. The organic halamine was found to have superior stability in solution and to exhibit adequate disinfectant potential over a period of 1 m...

journal_title：Applied and environmental microbiology

authors： Swango LJ,Wilt GR,Killen AD,Williams DE,Worley SD

更新日期：1987-12-01 00:00:00

abstract：:Three of 36 raw milk isolates of Yersinia enterocolitica produced enterotoxin in milk at 25 degrees C, but not at 4 degrees C. No strain tested could survive pasteurization. ...

journal_title：Applied and environmental microbiology

authors： Francis DW,Spaulding PL,Lovett J

更新日期：1980-07-01 00:00:00

abstract：:Clostridium acetobutylicum is not able to grow on glycerol as the sole carbon source since it cannot reoxidize the excess of NADH generated by glycerol catabolism. Nevertheless, when the pSPD5 plasmid, carrying the NADH-consuming 1,3-propanediol pathway from C. butyricum VPI 3266, was introduced into C. acetobutylicum...

journal_title：Applied and environmental microbiology

authors： González-Pajuelo M,Meynial-Salles I,Mendes F,Soucaille P,Vasconcelos I

更新日期：2006-01-01 00:00:00

abstract：:Hypoxia is encountered frequently by Candida albicans during systemic infection of the human host. We tested if hypoxia allows biofilm formation by C. albicans, which is a major cause of perseverance and antifungal resistance in C. albicans infections. Using an in vitro biofilm system, we unexpectedly discovered that ...

journal_title：Applied and environmental microbiology

authors： Stichternoth C,Ernst JF

更新日期：2009-06-01 00:00:00

abstract：:An anaerobic diplococcoid bacterium able to degrade hydrolyzable tannins was isolated from the ruminal fluid of a goat fed desmodium (Desmodium ovalifolium), a tropical legume which contains levels as high as 17% condensed tannins. This strain grew under anaerobic conditions in the presence of up to 30 g of tannic aci...

journal_title：Applied and environmental microbiology

authors： Nelson KE,Pell AN,Schofield P,Zinder S

更新日期：1995-09-01 00:00:00

abstract：:Bacteria were isolated from lake water, and their ability to remain viable in a dilute, nutrient-deficient environment was tested by a method that permits suspension of test bacteria between two appressed microporous membranes in an aqueous environment. This approach permitted separation of the lake isolates into two ...

journal_title：Applied and environmental microbiology

authors： Sjogren RE,Gibson MJ

更新日期：1981-06-01 00:00:00

abstract：:Culture-independent molecular phylogenetic methods were used to explore the breadth of diversity and environmental distribution of members of the division-level "candidate" phylogenetic group WS6, recently discovered in a contaminated aquifer and with no cultivated representatives. A broad diversity of WS6-affiliated ...

journal_title：Applied and environmental microbiology

authors： Dojka MA,Harris JK,Pace NR

更新日期：2000-04-01 00:00:00

abstract：:A 2.2-kb fragment containing a replicon from pBtoxis, the large plasmid that encodes the insecticidal endotoxins of Bacillus thuringiensis subsp. israelensis, was identified, cloned, and sequenced. This fragment contains cis elements, including iterons, found in replication origins of other large plasmids and suggests...

journal_title：Applied and environmental microbiology

authors： Tang M,Bideshi DK,Park HW,Federici BA

更新日期：2006-11-01 00:00:00

abstract：:The natural abundance of stable carbon isotopes measured in bacterial nucleic acids extracted from estuarine bacterial concentrates was used to trace sources of organic matter for bacteria in aquatic environments. The stable carbon isotope ratios of Pseudomonas aeruginosa and nucleic acids extracted from cultures rese...

journal_title：Applied and environmental microbiology

authors： Coffin RB,Velinsky DJ,Devereux R,Price WA,Cifuentes LA

更新日期：1990-07-01 00:00:00

abstract：:The circulation of human parechoviruses (HPeVs) in the population was studied by environmental surveillance comprising of molecular analyses of sewage samples (n = 89) that were collected from 15 different locations in The Netherlands. Samples were taken from sewage originating from schools (n = 9) or from parts of mu...

journal_title：Applied and environmental microbiology

authors： Lodder WJ,Wuite M,de Roda Husman AM,Rutjes SA

更新日期：2013-10-01 00:00:00

abstract：:Cells of the gram-negative bacterium Ralstonia sp. strain SBUG 290 grown in the presence of biphenyl are able to cooxidize dibenzofuran which has been 1,2-hydroxylated. Meta cleavage of the 1, 2-dihydroxydibenzofuran between carbon atoms 1 and 9b produced 2-hydroxy-4-(3'-oxo-3'H-benzofuran-2'-yliden)but-2-enoic acid, ...

journal_title：Applied and environmental microbiology

authors： Becher D,Specht M,Hammer E,Francke W,Schauer F

更新日期：2000-10-01 00:00:00

abstract：:Portions of published procedures for measurement of ergosterol content of decomposing plants were examined for their influence upon ergosterol yield. Common methods of treatment of plant samples prior to sterol extraction (e.g., oven drying, freezing, lyophilization) led to reduced recoveries of ergosterol (ca. 20 to ...

journal_title：Applied and environmental microbiology

authors： Newell SY,Arsuffi TL,Fallon RD

更新日期：1988-07-01 00:00:00

abstract：:Unspecific peroxygenases (UPOs) constitute a new family of fungal heme-thiolate enzymes in which there is high biotechnological interest. Although several thousand genes encoding hypothetical UPO-type proteins have been identified in sequenced fungal genomes and other databases, only a few UPO enzymes have been experi...

journal_title：Applied and environmental microbiology

authors： Linde D,Olmedo A,González-Benjumea A,Estévez M,Renau-Mínguez C,Carro J,Fernández-Fueyo E,Gutiérrez A,Martínez AT

更新日期：2020-03-18 00:00:00

abstract：:Antibody against nivalenol tetraacetate (tetra-Ac-NIV) was prepared by immunization of rabbits with triacetyl-15-pimelate-NIV conjugated to bovine serum albumin. By using tritiated tetra-Ac-NIV as the test ligand, antibody titers were demonstrated as early as 4 weeks after immunization. Useful antibody for radioimmuno...

journal_title：Applied and environmental microbiology

authors： Wang CR,Chu FS

更新日期：1991-04-01 00:00:00

abstract：:Molecular analysis of grassland rhizosphere soil has demonstrated complex and diverse bacterial communities, with resultant difficulties in detecting links between plant and bacterial communities. These studies have, however, analyzed "bulk" rhizosphere soil, rather than rhizoplane communities, which interact most clo...

journal_title：Applied and environmental microbiology

authors： Nunan N,Daniell TJ,Singh BK,Papert A,McNicol JW,Prosser JI

更新日期：2005-11-01 00:00:00

abstract：:Xanthomonas citri subsp. citri is the causal agent of citrus canker, which has a significant impact on citrus production. In this study, we characterized the galU gene of X. citri subsp. citri. Two galU mutants (F6 and D12) were identified in an X. citri subsp. citri EZ-Tn5 Tnp transposon library. ...

journal_title：Applied and environmental microbiology

authors： Guo Y,Sagaram US,Kim JS,Wang N

更新日期：2010-04-01 00:00:00

abstract：:This study was undertaken to find optimum conditions of tryptone, yeast extract, glucose, Tween 80, and incubation temperature for the growth of Lactobacillus casei YIT 9018 and to assess the effects of these factors by use of response surface methodology. A central composite design was used as an experimental design ...

journal_title：Applied and environmental microbiology

authors： Oh S,Rheem S,Sim J,Kim S,Baek Y

更新日期：1995-11-01 00:00:00

abstract：:Francisella tularensis is found in a wide variety of hosts and extrahost environments, making culture recovery a diagnostic challenge. Here we demonstrate improved recovery times and good sensitivity (90%) when cultures were inoculated on the site of an investigation using fresh tissues. For contaminated specimens, an...

journal_title：Applied and environmental microbiology

authors： Petersen JM,Schriefer ME,Gage KL,Montenieri JA,Carter LG,Stanley M,Chu MC

更新日期：2004-06-01 00:00:00

abstract：:The quorum sensing (QS) system controls bacterial group behaviors in response to cell density. In vibrios, LuxR and AphA are two master QS regulators (MQSRs) controlling gene expression in response to high or low cell density. Other regulators involved in the regulation of these two MQSRs and QS pathways remain to be ...

journal_title：Applied and environmental microbiology

authors： Gao X,Wang X,Mao Q,Xu R,Zhou X,Ma Y,Liu Q,Zhang Y,Wang Q

更新日期：2018-05-31 00:00:00

abstract：:The lytic action of glycerol and sucrose esters of fatty acids with different carbon chain lengths on the exponentially growing cells of Bacillus subtilis 168 was investigated. Of each series of esters, glycerol dodecanoate and sucrose hexadecanoate were the most active. Lysis at 1 h after the addition of 0.1 mM glyce...

journal_title：Applied and environmental microbiology

authors： Tsuchido T,Ahn YH,Takano M

更新日期：1987-03-01 00:00:00

abstract：:To cause an infection, bacteriophages must penetrate the alginate exopolysaccharide of Pseudomonas aeruginosa to reach the bacterial surface. Despite a lack of intrinsic motility, phage were shown to diffuse through alginate gels at alginate concentrations up to 8% (wt/vol) and to bring about a 2-log reduction in the ...

journal_title：Applied and environmental microbiology

authors： Hanlon GW,Denyer SP,Olliff CJ,Ibrahim LJ

更新日期：2001-06-01 00:00:00