Use of the yeast Pichia pastoris as an expression host for secretion of enterocin L50, a leaderless two-peptide (L50A and L50B) bacteriocin from Enterococcus faecium L50.

abstract：:Norovirus is the leading cause of food-related illness in the United States, and contamination of ready-to-eat items by food handlers poses a high risk for disease. This study reports the in vitro (suspension test) and in vivo (fingerpad protocol) assessments of a new ethanol-based hand sanitizer containing a synergis...

journal_title：Applied and environmental microbiology

authors： Macinga DR,Sattar SA,Jaykus LA,Arbogast JW

更新日期：2008-08-01 00:00:00

abstract：:Xylella fastidiosa is a gram-negative, xylem-limited bacterium affecting economically important crops (e.g., grapevine, citrus, and coffee). The citrus variegated chlorosis (CVC) strain of X. fastidiosa is the causal agent of this severe disease of citrus in Brazil and represents the first plant-pathogenic bacterium f...

journal_title：Applied and environmental microbiology

authors： Monteiro PB,Teixeira DC,Palma RR,Garnier M,Bové JM,Renaudin J

更新日期：2001-05-01 00:00:00

abstract：:A highly efficient, rapid, and reliable PCR-based method for distinguishing Lactococcus lactis subspecies (L. lactis subsp. lactis and L. lactis subsp. cremoris) is described. Primers complementary to positions in the glutamate decarboxylase gene have been constructed. PCR analysis with extracted DNA or with cells of ...

journal_title：Applied and environmental microbiology

authors： Nomura M,Kobayashi M,Okamoto T

更新日期：2002-05-01 00:00:00

abstract：:Hartmannella vermiformis, a common amoebal inhabitant of potable-water systems, supports intracellular multiplication of Legionella pneumophila and is probably important in the transportation and amplification of legionellae within these systems. To provide a practical guide for decontamination of potable-water system...

journal_title：Applied and environmental microbiology

authors： Kuchta JM,Navratil JS,Shepherd ME,Wadowsky RM,Dowling JN,States SJ,Yee RB

更新日期：1993-12-01 00:00:00

abstract：:Many food-grade bacteria produce exopolysaccharides (EPS) that affect the texture of fermented food products and that may be involved in probiotic properties. Propionibacterium freudenreichii is a Gram-positive food-grade bacterium with reported probiotic capabilities that is widely used as starter in Swiss-type chees...

journal_title：Applied and environmental microbiology

authors： Deutsch SM,Le Bivic P,Hervé C,Madec MN,LaPointe G,Jan G,Le Loir Y,Falentin H

更新日期：2010-05-01 00:00:00

abstract：:Penicillium decumbens is able to epoxidize cis-propenylphosphonic acid (cPA) to produce the antibiotic fosfomycin [FOM; also referred to as phosphonomycin and (-)-cis-1,2-epoxypropylphosphonic acid], a bioconversion of considerable commercial significance. We sought to improve the efficiency of the process by overexpr...

journal_title：Applied and environmental microbiology

authors： Watanabe M,Sumida N,Murakami S,Anzai H,Thompson CJ,Tateno Y,Murakami T

更新日期：1999-03-01 00:00:00

abstract：:Fructansucrase enzymes polymerize the fructose moiety of sucrose into levan or inulin fructans, with beta(2-6) and beta(2-1) linkages, respectively. The probiotic bacterium Lactobacillus johnsonii strain NCC 533 possesses a single fructansucrase gene (open reading frame AAS08734) annotated as a putative levansucrase p...

journal_title：Applied and environmental microbiology

authors： Anwar MA,Kralj S,van der Maarel MJ,Dijkhuizen L

更新日期：2008-06-01 00:00:00

abstract：:Knowledge of the mechanism of pressure-induced inactivation of microorganisms could be helpful in defining an effective, relatively mild pressure treatment as a means of decontamination, especially in combination with other physical treatments or antimicrobial agents. We have studied the effect of high pressure on Lac...

journal_title：Applied and environmental microbiology

authors： Wouters PC,Glaasker E,Smelt JP

更新日期：1998-02-01 00:00:00

abstract：:Sulfidogenic consortia enriched from an estuarine sediment were maintained on either 2-, 3-, or 4-chlorophenol as the only source of carbon and energy for over 5 years. The enrichment culture on 4-chlorophenol was the most active and this consortium was selected for further characterization. Utilization of chloropheno...

journal_title：Applied and environmental microbiology

authors： Häggblom MM,Young LY

更新日期：1995-04-01 00:00:00

abstract：:Trichoderma reesei can produce up to 100 g/liter of extracellular proteins. The major and industrially relevant products are cellobiohydrolase I (CBHI) and the hemicellulase XYNI. The genes encoding both enzymes are transcriptionally activated by the regulatory protein Xyr1. The first 850 nucleotides of the cbh1 promo...

journal_title：Applied and environmental microbiology

authors： Kiesenhofer DP,Mach RL,Mach-Aigner AR

更新日期：2017-12-15 00:00:00

abstract：:Helaeomyia petrolei larvae isolated from the asphalt seeps of Rancho La Brea in Los Angeles, Calif., were examined for microbial gut contents. Standard counts on Luria-Bertani, MacConkey, and blood agar plates indicated ca. 2 x 10(5) heterotrophic bacteria per larva. The culturable bacteria represented 15 to 20% of th...

journal_title：Applied and environmental microbiology

authors： Kadavy DR,Plantz B,Shaw CA,Myatt J,Kokjohn TA,Nickerson KW

更新日期：1999-04-01 00:00:00

abstract：:An extracellular serine proteinase from Brevibacterium linens ATCC 9174 was purified to homogeneity. pH and temperature optima were 8.5 and 50(deg)C, respectively. The results for the molecular mass of the proteinase were 56 kDa by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and 126 kDa by gel filtration...

journal_title：Applied and environmental microbiology

authors： Rattray FP,Bockelmann W,Fox PF

更新日期：1995-09-01 00:00:00

abstract：:The root-colonizing bacterium Pseudomonas fluorescens CHA0 was used to construct an oxygen-responsive biosensor. An anaerobically inducible promoter of Pseudomonas aeruginosa, which depends on the FNR (fumarate and nitrate reductase regulation)-like transcriptional regulator ANR (anaerobic regulation of arginine deimi...

journal_title：Applied and environmental microbiology

authors： Højberg O,Schnider U,Winteler HV,Sørensen J,Haas D

更新日期：1999-09-01 00:00:00

abstract：:This study evaluated the influence of parameters relevant for cheese making on histamine formation by Streptococcus thermophilus. Strains possessing a histidine decarboxylase (hdcA) gene represented 6% of the dairy isolates screened. The most histaminogenic, S. thermophilus PRI60, exhibited in skim milk a high basal l...

journal_title：Applied and environmental microbiology

authors： Rossi F,Gardini F,Rizzotti L,La Gioia F,Tabanelli G,Torriani S

更新日期：2011-04-01 00:00:00

abstract：:Pure and mixed cultures of Methylophaga sulfidovorans and Thiobacillus thioparus T5 were grown in continuous cultures on either dimethyl sulfide, dimethyl sulfide and H(inf2)S, or H(inf2)S and methanol. In pure cultures, M. sulfidovorans showed a lower affinity for sulfide than T. thioparus T5. Mixed cultures, grown o...

journal_title：Applied and environmental microbiology

authors： De Zwart J,Sluis J,Kuenen JG

更新日期：1997-08-01 00:00:00

abstract：:The objective of this study was to determine the effect of high pressure (HP) on the inactivation of microbial contaminants in Cheddar cheese (Escherichia coli K-12, Staphylococcus aureus ATCC 6538, and Penicillium roqueforti IMI 297987). Initially, cheese slurries inoculated with E. coli, S. aureus, and P. roqueforti...

journal_title：Applied and environmental microbiology

authors： O'Reilly CE,O'Connor PM,Kelly AL,Beresford TP,Murphy PM

更新日期：2000-11-01 00:00:00

abstract：:Anaerobic acetate degradation at 70 degrees C and at 55 degrees C (as a reference) was studied by running laboratory upflow anaerobic sludge blanket (UASB) reactors inoculated with mesophilic granular sludge. In UASB reactors fed with acetate-containing media (3 g of chemical oxygen demand [COD] per liter, correspondi...

journal_title：Applied and environmental microbiology

authors： Rintala J,Lepistö S,Ahring B

更新日期：1993-06-01 00:00:00

abstract：:Microorganisms associated with the stems and roots of nonnodulated (Nod(-)), wild-type nodulated (Nod(+)), and hypernodulated (Nod(++)) soybeans [Glycine max (L.) Merril] were analyzed by ribosomal intergenic transcribed spacer analysis (RISA) and automated RISA (ARISA). RISA of stem samples detected no bands specific...

journal_title：Applied and environmental microbiology

authors： Ikeda S,Rallos LE,Okubo T,Eda S,Inaba S,Mitsui H,Minamisawa K

更新日期：2008-09-01 00:00:00

abstract：:Fermentative production of succinic acid from glucose by Escherichia coli was significantly increased by overexpression of phosphoenolpyruvate carboxylase. In contrast, overexpression of phosphoenolpyruvate carboxykinase had no effect. Under optimized conditions, induction of the carboxylase resulted in a 3.5-fold inc...

journal_title：Applied and environmental microbiology

authors： Millard CS,Chao YP,Liao JC,Donnelly MI

更新日期：1996-05-01 00:00:00

abstract：:Unlike general peroxidases, Pleurotus ostreatus MnP2 was reported to have a unique property of direct oxidization of high-molecular-weight compounds, such as Poly R-478 and RNase A. To elucidate the mechanism for oxidation of polymeric substrates by MnP2, a series of mutant enzymes were produced by using a homologous ...

journal_title：Applied and environmental microbiology

authors： Tsukihara T,Honda Y,Sakai R,Watanabe T,Watanabe T

更新日期：2008-05-01 00:00:00

abstract：:A predictive and feedback proportional control algorithm, developed for fed-batch fermentations and described in a companion paper (G. L. Kleman, J. J. Chalmers, G. W. Luli, and W. R. Strohl, Appl. Environ. Microbiol. 57:910-917, 1991), was used in this work to control a continuous culture on the basis of the soluble-...

journal_title：Applied and environmental microbiology

authors： Kleman GL,Chalmers JJ,Luli GW,Strohl WR

更新日期：1991-04-01 00:00:00

abstract：:Columns were packed with clean quartz sand, sterilized, and inoculated with different strains of bacteria, which multiplied within the sand at the expense of a continuous supply of fresh nutrient medium. The saturated hydraulic conductivity (HCsat) of the sand was monitored over time. Among the four bacterial strains ...

journal_title：Applied and environmental microbiology

authors： Vandevivere P,Baveye P

更新日期：1992-05-01 00:00:00

abstract：:A homokaryotic, clavine alkaloid-producing strain of ergot, Claviceps sp. strain SD 58, was used in an attempt to demonstrate parasexuality. Genetically marked auxotrophic strains were produced by mutation with N-methyl-N'-nitro-N-nitrosoguanidine. Protoplast fusion of pairs of unlike doubly auxotrophic strains and is...

journal_title：Applied and environmental microbiology

authors： Brauer KL,Robbers JE

更新日期：1987-01-01 00:00:00

abstract：:Drinking water quality management requires early warning tools which enable water supply companies to detect quickly and to forecast degradation of the microbial quality of drinking water during its transport throughout distribution systems. This study evaluated the feasibility of assessing, in real time, drinking wat...

journal_title：Applied and environmental microbiology

authors： Delille A,Quilès F,Humbert F

更新日期：2007-09-01 00:00:00

abstract：:The thermophilic acetogenic bacterium Thermoanaerobacter kivui, previously described not to use carbon monoxide as a carbon and energy source, was adapted to grow on CO. This was achieved by using a preculture grown on H2 plus CO2 and by increasing the CO concentration in small, 10% increments.T. kivui was finally abl...

journal_title：Applied and environmental microbiology

authors： Weghoff MC,Müller V

更新日期：2016-04-04 00:00:00

abstract：:The transport of branched-chain amino acids in Streptococcus thermophilus was energy dependent. The metabolic inhibitors of glycolysis and ATPase enzymes were active, but the proton-conducting uncouplers were not. Transport was optimal at temperatures of between 30 and 45 degrees C and at pH 7.0 for the three amino ac...

journal_title：Applied and environmental microbiology

authors： Akpemado KM,Bracquart PA

更新日期：1983-01-01 00:00:00

abstract：:In the present work, we aimed at determining the relationship between the hepatitis A virus (HAV) numbers in imported frozen coquina clams involved in two hepatitis outbreaks, as well as the risk for human health. Due to HAV unculturability, a standardized TaqMan real-time reverse transcription-PCR controlling the vir...

journal_title：Applied and environmental microbiology

authors： Pintó RM,Costafreda MI,Bosch A

更新日期：2009-12-01 00:00:00

abstract：:[This corrects the article on p. 1301 in vol. 50.]. ...

journal_title：Applied and environmental microbiology

authors：

更新日期：1986-03-01 00:00:00

abstract：:Arthrobacter viscosus NRRL B-1973 produces its viscous extracellular polysaccharides (EPS) when grown on media containing xylose or enzymatic xylan hydrolysates. Crude EPS formation from xylose averaged 12 g/liter when initial culture pH was adjusted to 8.0 and total nitrogen was limited to 0.03%. Purified EPS from pe...

journal_title：Applied and environmental microbiology

authors： Novak JS,Tanenbaum SW,Nakas JP

更新日期：1992-11-01 00:00:00

abstract：:The relationship between physiochemical surface parameters and adhesion of bacterial cells to negatively charged polystyrene was studied. Cell surface hydrophobicity and electrokinetic potential were determined by contact angle measurement and electrophoresis, respectively. Both parameters influence cell adhesion. The...

journal_title：Applied and environmental microbiology

authors： van Loosdrecht MC,Lyklema J,Norde W,Schraa G,Zehnder AJ

更新日期：1987-08-01 00:00:00