Enhanced production of succinic acid by overexpression of phosphoenolpyruvate carboxylase in Escherichia coli.

abstract：:The relationship between the production of reduced oxygen species, hydrogen peroxide (H(2)O(2)), superoxide (O(2)), and hydroxyl radical (.OH), and the oxidation of synthetic lignin to CO(2) was studied in whole cultures of the white-rot fungus Phanerochaete chrysosporium Burds. The kinetics of the synthesis of H(2)O(...

journal_title：Applied and environmental microbiology

authors： Faison BD,Kirk TK

更新日期：1983-11-01 00:00:00

abstract：:Extracellular electron transfer (EET) is a strategy for respiration in which electrons generated from metabolism are moved outside the cell to a terminal electron acceptor, such as iron or manganese oxide. EET has primarily been studied in two model systems, Shewanella oneidensis and Geobacter sulfurreducens Metal red...

journal_title：Applied and environmental microbiology

authors： Conley BE,Intile PJ,Bond DR,Gralnick JA

更新日期：2018-11-15 00:00:00

abstract：:Patulin production by Penicillium griseofulvum was monitored with Sep-Pak cartridges and high-pressure liquid chromatography. Determination and quantification of this metabolite proved to be very simple, and our method saved time and a large amount of organic solvents. ...

journal_title：Applied and environmental microbiology

authors： Torres M,Sanchis V,Riba M,Canela R

更新日期：1986-01-01 00:00:00

abstract：:Ammonia-oxidizing bacteria (AOB) are thought to contribute significantly to N2O production and methane oxidation in soils. Most of our knowledge derives from experiments with Nitrosomonas europaea, which appears to be of minor importance in most soils compared to Nitrosospira spp. We have conducted a comparative study...

journal_title：Applied and environmental microbiology

authors： Jiang QQ,Bakken LR

更新日期：1999-06-01 00:00:00

abstract：:Here, we describe a novel clade within Ensifer meliloti and consider how geographic and ecological isolation contributed to the limited distribution of this group. Members of the genus Ensifer are best known for their ability to form nitrogen-fixing symbioses with forage legumes of three related genera, Medicago L., M...

journal_title：Applied and environmental microbiology

authors： Eardly B,Elia P,Brockwell J,Golemboski D,van Berkum P

更新日期：2017-05-01 00:00:00

abstract：:The occurrence of pigmented bacteria in potable water, from raw source water through treatment to distribution water, including dead-end locations, was compared at sample sites in a large municipal water system. Media used to enumerate heterotrophic bacteria and differentiate pigmented colonies were standard method pl...

journal_title：Applied and environmental microbiology

authors： Reasoner DJ,Blannon JC,Geldreich EE,Barnick J

更新日期：1989-04-01 00:00:00

abstract：:Laccase is a copper-containing polyphenol oxidase that has great potential in industrial and biotechnological applications. Previous research has suggested that fungal laccase may be involved in the defense against oxidative stress, but there is little direct evidence supporting this hypothesis, and the mechanism by w...

journal_title：Applied and environmental microbiology

authors： Yang Y,Fan F,Zhuo R,Ma F,Gong Y,Wan X,Jiang M,Zhang X

更新日期：2012-08-01 00:00:00

abstract：:Quantitative assessment of growth of filamentous microorganisms, such as streptomycetes, is generally restricted to determination of dry weight. Here, we describe a straightforward methylene blue-based sorption assay to monitor microbial growth quantitatively, simply, and rapidly. The assay is equally applicable to un...

journal_title：Applied and environmental microbiology

authors： Fischer M,Sawers RG

更新日期：2013-07-01 00:00:00

abstract：:Oxolinic acid (OA), a quinolone, inhibits the activity of DNA gyrase composed of GyrA and GyrB and shows antibacterial activity against Burkholderia glumae. Since B. glumae causes bacterial seedling rot and grain rot of rice, both of which are devastating diseases, the emergence of OA-resistant bacteria has important ...

journal_title：Applied and environmental microbiology

authors： Maeda Y,Kiba A,Ohnishi K,Hikichi Y

更新日期：2004-09-01 00:00:00

abstract：:Francisella tularensis is found in a wide variety of hosts and extrahost environments, making culture recovery a diagnostic challenge. Here we demonstrate improved recovery times and good sensitivity (90%) when cultures were inoculated on the site of an investigation using fresh tissues. For contaminated specimens, an...

journal_title：Applied and environmental microbiology

authors： Petersen JM,Schriefer ME,Gage KL,Montenieri JA,Carter LG,Stanley M,Chu MC

更新日期：2004-06-01 00:00:00

abstract：:Lactocin 705 is a bacteriocin whose activity depends upon the complementation of two peptides, termed Lac705alpha and Lac705beta. Neither Lac705alpha nor Lac705beta displayed bacteriocin activity by itself when the growth of sensitive cells was monitored. To obtain molecular insights into the lactocin 705 mechanism of...

journal_title：Applied and environmental microbiology

authors： Castellano P,Vignolo G,Farías RN,Arrondo JL,Chehín R

更新日期：2007-01-01 00:00:00

abstract：:The genus Aeromonas includes some species that have now been identified as human pathogens of significant medical importance. We investigated the ability of 13 selected Aeromonas strains belonging to nine species isolated from clinical cases (n = 5), environmental waters (n = 5), and fish (n = 3) to adhere to and tran...

journal_title：Applied and environmental microbiology

authors： Hatje E,Neuman C,Katouli M

更新日期：2014-01-01 00:00:00

abstract：:We developed a chemiluminometric assay of beta-galactosidase in coliform bacteria, using a phenylgalactose-substituted 1,2-dioxetane derivative as a substrate. Permeabilization of cells is required to ensure the efficient cellular uptake of this compound. By this method, one coliform seeded in 100 ml of sterile water ...

journal_title：Applied and environmental microbiology

authors： Van Poucke SO,Nelis HJ

更新日期：1995-12-01 00:00:00

abstract：:Several natural isolates of Escherichia coli O157:H7 have previously been shown to exhibit stationary-phase-dependent variation in their resistance to inactivation by high hydrostatic pressure. In this report we demonstrate that loss of the stationary-phase-inducible sigma factor RpoS resulted in decreased resistance ...

journal_title：Applied and environmental microbiology

authors： Robey M,Benito A,Hutson RH,Pascual C,Park SF,Mackey BM

更新日期：2001-10-01 00:00:00

abstract：:Mercury is a redox-active heavy metal that reacts with active thiols and depletes cellular antioxidants. Active resistance to the mercuric ion is a widely distributed trait among bacteria and results from the action of mercuric reductase (MerA). Protein phylogenetic analysis of MerA in bacteria indicated the occurrenc...

journal_title：Applied and environmental microbiology

authors： Simbahan J,Kurth E,Schelert J,Dillman A,Moriyama E,Jovanovich S,Blum P

更新日期：2005-12-01 00:00:00

abstract：:To survive in various environments, from host tissue to soil, opportunistic bacterial pathogens must be metabolically flexible and able to use a variety of nutrient sources. We are interested in Pseudomonas aeruginosa's catabolism of quaternary amine compounds that are prevalent in association with eukaryotes. Carniti...

journal_title：Applied and environmental microbiology

authors： Meadows JA,Wargo MJ

更新日期：2013-06-01 00:00:00

abstract：:The isophthalate (IPA) degradation gene cluster (iphACBDR) responsible for the conversion of IPA into protocatechuate (PCA) was isolated from Comamonas sp. strain E6, which utilizes phthalate isomers as sole carbon and energy sources via the PCA 4,5-cleavage pathway. Based on amino acid sequence similarity, the iphA, ...

journal_title：Applied and environmental microbiology

authors： Fukuhara Y,Inakazu K,Kodama N,Kamimura N,Kasai D,Katayama Y,Fukuda M,Masai E

更新日期：2010-01-01 00:00:00

abstract：:Phylogenetic relationships, diversity, and in situ identification of spirochetes in the gut of the termite Neotermes koshunensis were examined without cultivation, with an emphasis on ectosymbionts attached to flagellated protists. Spirochetes in the gut microbial community investigated so far are related to the genus...

journal_title：Applied and environmental microbiology

authors： Noda S,Ohkuma M,Yamada A,Hongoh Y,Kudo T

更新日期：2003-01-01 00:00:00

abstract：:Vibrio vulnificus naturally inhabits a variety of aquatic organisms, including oysters, and is the leading cause of seafood-related death in the United States. Strains of this bacterium are genetically classified into environmental (E) and clinical (C) genotypes, which correlate with source of isolation. E-genotype st...

journal_title：Applied and environmental microbiology

authors： Williams TC,Ayrapetyan M,Oliver JD

更新日期：2014-03-01 00:00:00

abstract：:Dehalococcoides sp. strain BAV1 couples growth with the reductive dechlorination of vinyl chloride (VC) to ethene. Degenerate primers targeting conserved regions in reductive dehalogenase (RDase) genes were designed and used to PCR amplify putative RDase genes from strain BAV1. Seven unique RDase gene fragments were i...

journal_title：Applied and environmental microbiology

authors： Krajmalnik-Brown R,Hölscher T,Thomson IN,Saunders FM,Ritalahti KM,Löffler FE

更新日期：2004-10-01 00:00:00

abstract：:Alternariol and alternariol methyl ether were tested in the Ames Salmonella typhimurium assay, and both were shown, with and without metabolic activation, to be nonmutagenic to strains TA98 and TA100. The finding of other investigators that alternariol methyl ether is weakly mutagenic to TA98 without metabolic activat...

journal_title：Applied and environmental microbiology

authors： Davis VM,Stack ME

更新日期：1994-10-01 00:00:00

abstract：:Methane is an undesirable end product of rumen fermentative activity because of associated environmental impacts and reduced host feed efficiency. Our study characterized the rumen microbial methanogenic community in beef cattle divergently selected for phenotypic residual feed intake (RFI) while offered a high-forage...

journal_title：Applied and environmental microbiology

authors： Carberry CA,Waters SM,Kenny DA,Creevey CJ

更新日期：2014-01-01 00:00:00

abstract：:The industrial production of beta-carotene with the zygomycete Blakeslea trispora involves the joint cultivation of mycelia of opposite sex in the presence of beta-ionone and other chemical activators. We have obtained improved strains by mutation and heterokaryosis. We chose wild strains on the basis of their growth ...

journal_title：Applied and environmental microbiology

authors： Mehta BJ,Obraztsova IN,Cerdá-Olmedo E

更新日期：2003-07-01 00:00:00

abstract：:The circulation of human parechoviruses (HPeVs) in the population was studied by environmental surveillance comprising of molecular analyses of sewage samples (n = 89) that were collected from 15 different locations in The Netherlands. Samples were taken from sewage originating from schools (n = 9) or from parts of mu...

journal_title：Applied and environmental microbiology

authors： Lodder WJ,Wuite M,de Roda Husman AM,Rutjes SA

更新日期：2013-10-01 00:00:00

abstract：:The practical task of adapting an original potentiometric technique to the bacteriological analysis of water is discussed. Various laboratory strains of organisms belonging to the usual aquatic flora were inoculated one by one in a minimal lactose broth supplied with lipoic (thioctic) acid. The time evolution of the r...

journal_title：Applied and environmental microbiology

authors： Charriere G,Jouenne T,Lemeland JF,Selegny E,Junter GA

更新日期：1984-01-01 00:00:00

abstract：:Marine microbiologists commonly assay lipase activities by using a synthetic fluorescent analog, 4-methylumbelliferyl (MUF)-oleate. The technique is convenient, but it is considered to be unspecific because of the structure of this analog. This study reports the design of a new specific and sensitive lipase assay base...

journal_title：Applied and environmental microbiology

authors： Bourguet N,Torréton JP,Galy O,Arondel V,Goutx M

更新日期：2003-12-01 00:00:00

abstract：:A database was probed with artificial neural network (ANN) and multivariate logistic regression (MLR) models to investigate the efficacy of predicting PCR-identified human adenovirus (ADV), Norwalk-like virus (NLV), and enterovirus (EV) presence or absence in shellfish harvested from diverse countries in Europe (Spain...

journal_title：Applied and environmental microbiology

authors： Brion G,Viswanathan C,Neelakantan TR,Lingireddy S,Girones R,Lees D,Allard A,Vantarakis A

更新日期：2005-09-01 00:00:00

abstract：:Laboratory strains of Saccharomyces cerevisiae have been widely used as a model for studying eukaryotic cells and mapping the molecular mechanisms of many different human diseases. Industrial wine yeasts, on the other hand, have been selected on the basis of their adaptation to stringent environmental conditions and t...

journal_title：Applied and environmental microbiology

authors： Pizarro FJ,Jewett MC,Nielsen J,Agosin E

更新日期：2008-10-01 00:00:00

abstract：:The Tinto River (Huelva, southwestern Spain) is an extreme environment with a rather constant acidic pH along the entire river and a high concentration of heavy metals. The extreme conditions of the Tinto ecosystem are generated by the metabolic activity of chemolithotrophic microorganisms thriving in the rich complex...

journal_title：Applied and environmental microbiology

authors： González-Toril E,Llobet-Brossa E,Casamayor EO,Amann R,Amils R

更新日期：2003-08-01 00:00:00

abstract：:The well-established killing of bacteria by copper surfaces, also called contact killing, is currently believed to be a combined effect of bacterial contact with the copper surface and the dissolution of copper, resulting in lethal bacterial damage. Iron can similarly be released in ionic form from iron surfaces and w...

journal_title：Applied and environmental microbiology

authors： Mathews S,Kumar R,Solioz M

更新日期：2015-09-01 00:00:00