Abstract:
:Here, we describe a novel clade within Ensifer meliloti and consider how geographic and ecological isolation contributed to the limited distribution of this group. Members of the genus Ensifer are best known for their ability to form nitrogen-fixing symbioses with forage legumes of three related genera, Medicago L., Melilotus Mill., and Trigonella L., which are members of the tribe Trifolieae. These legumes have a natural distribution extending from the Mediterranean Basin through western Asia, where there is an unsurpassed number of species belonging to these genera. Trigonella suavissima L. is unusual in that it is the only species in the tribe Trifolieae that is native to Australia. We compared the genetic diversity and taxonomic placement of rhizobia nodulating T. suavissima with those of members of an Ensifer reference collection. Our goal was to determine if the T. suavissima rhizobial strains, like their plant host, are naturally limited to the Australian continent. We used multilocus sequence analysis to estimate the genetic relatedness of 56 T. suavissima symbionts to 28 Ensifer reference strains. Sequence data were partitioned according to the replicons in which the loci are located. The results were used to construct replicon-specific phylogenetic trees. In both the chromosomal and chromid trees, the Australian strains formed a distinct clade within E. meliloti The strains also shared few alleles with Ensifer reference strains from other continents. Carbon source utilization assays revealed that the strains are also unusual in their ability to utilize 2-oxoglutarate as a sole carbon source. A strategy was outlined for locating similar strains elsewhere.IMPORTANCE In this study, we employed a biogeographical approach to investigate the origins of a symbiotic relationship between an Australian legume and its nitrogen-fixing rhizobia. The question of the ancestral origins of these symbionts is based on the observation that the legume host is not closely related to other native Australian legumes. Previous research has shown that the legume host Trigonella suavissima is instead closely related to legumes native to the Mediterranean Basin and western Asia, suggesting that it may have been introduced in Australia from those regions. This led to the question of whether its rhizobia may have been introduced as well. In this study, we were unable to find persuasive evidence supporting this hypothesis. Instead, our results suggest either that the T. suavissima rhizobia are native to Australia or that our methods for locating their close relatives elsewhere are inadequate. A strategy to investigate the latter alternative is proposed.
journal_name
Appl Environ Microbioljournal_title
Applied and environmental microbiologyauthors
Eardly B,Elia P,Brockwell J,Golemboski D,van Berkum Pdoi
10.1128/AEM.03446-16subject
Has Abstractpub_date
2017-05-01 00:00:00issue
10eissn
0099-2240issn
1098-5336pii
AEM.03446-16journal_volume
83pub_type
杂志文章abstract::In Europe, Borrelia burgdorferi genospecies causing Lyme borreliosis are mainly transmitted by the tick Ixodes ricinus. Since its discovery, B. burgdorferi has been the subject of many epidemiological studies to determine its prevalence and the distribution of the different genospecies in ticks. In the current study w...
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pub_type: 杂志文章,meta分析
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journal_title:Applied and environmental microbiology
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更新日期:1981-03-01 00:00:00
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pub_type: 杂志文章
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更新日期:1980-04-01 00:00:00
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更新日期:2001-01-01 00:00:00
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更新日期:1994-08-01 00:00:00
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更新日期:2006-02-01 00:00:00
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journal_title:Applied and environmental microbiology
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doi:10.1128/AEM.32.4.494-497.1976
更新日期:1976-10-01 00:00:00
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pub_type: 杂志文章,评审
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更新日期:2019-07-01 00:00:00
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journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/aem.69.8.4697-4705.2003
更新日期:2003-08-01 00:00:00
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journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.48.1.5-9.1984
更新日期:1984-07-01 00:00:00
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journal_title:Applied and environmental microbiology
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更新日期:2003-09-01 00:00:00