Increasing the carbon flux toward synthesis of short-chain-length--medium-chain-length polyhydroxyalkanoate in the peroxisome of Saccharomyces cerevisiae through modification of the beta-oxidation cycle.

Abstract:

:Short-chain-length-medium-chain-length polyhydroxyalkanoates were synthesized in Saccharomyces cerevisiae from intermediates of the beta-oxidation cycle by expressing the polyhydroxyalkanoate synthases from Aeromonas caviae and Ralstonia eutropha in the peroxisomes. The quantity of polymer produced was increased by using a mutant of the beta-oxidation-associated multifunctional enzyme with low dehydrogenase activity toward R-3-hydroxybutyryl coenzyme A.

journal_name

Appl Environ Microbiol

authors

De Oliveira VC,Maeda I,Delessert S,Poirier Y

doi

10.1128/AEM.70.9.5685-5687.2004

keywords:

subject

Has Abstract

pub_date

2004-09-01 00:00:00

pages

5685-7

issue

9

eissn

0099-2240

issn

1098-5336

pii

70/9/5685

journal_volume

70

pub_type

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