Abstract:
:Short-chain-length-medium-chain-length polyhydroxyalkanoates were synthesized in Saccharomyces cerevisiae from intermediates of the beta-oxidation cycle by expressing the polyhydroxyalkanoate synthases from Aeromonas caviae and Ralstonia eutropha in the peroxisomes. The quantity of polymer produced was increased by using a mutant of the beta-oxidation-associated multifunctional enzyme with low dehydrogenase activity toward R-3-hydroxybutyryl coenzyme A.
journal_name
Appl Environ Microbioljournal_title
Applied and environmental microbiologyauthors
De Oliveira VC,Maeda I,Delessert S,Poirier Ydoi
10.1128/AEM.70.9.5685-5687.2004keywords:
subject
Has Abstractpub_date
2004-09-01 00:00:00pages
5685-7issue
9eissn
0099-2240issn
1098-5336pii
70/9/5685journal_volume
70pub_type
杂志文章abstract::Low-temperature ecosystems represent the largest biosphere on Earth, and yet our understanding of the roles of bacteriophages in these systems is limited. Here, the influence of the cold-active filamentous phage SW1 on the phenotype and gene transcription of its host, Shewanella piezotolerans WP3 (WP3), was investigat...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.01675-13
更新日期:2013-11-01 00:00:00
abstract::Microbial communities in extreme environments often have low diversity and specialized physiologies suggesting a limited resistance to change. The McMurdo Dry Valleys (MDV) are a microbially dominated, extreme ecosystem currently undergoing climate change-induced disturbances, including the melting of massive buried i...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.03414-13
更新日期:2014-05-01 00:00:00
abstract::Enterohemorrhagic and enteropathogenic Escherichia coli (EHEC and EPEC, respectively) strains are closely related human pathogens that are responsible for food-borne epidemics in many countries. Integration host factor (IHF) and the locus of enterocyte effacement-encoded regulator (Ler) are needed for the expression o...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.70.9.5274-5282.2004
更新日期:2004-09-01 00:00:00
abstract::The steady-state effect of 2,5,2',5'-tetrachlorobiphenyl (TCBP) on the green alga Selenastrum capricornutum was investigated in a P-limited two-stage chemostat system. The partition coefficient of this polychlorinated biphenyl congener was 5.9 x 10 in steady-state cultures. At a cellular TCBP concentration of 12.2 x 1...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.54.6.1394-1398.1988
更新日期:1988-06-01 00:00:00
abstract::Cultures of 10 different bacteria were used to serve as food sources for axenically grown Acanthamoeba castellanii, Acanthamoeba polyphaga, and Hartmannella vermiformis. The nonpigmented enterobacteriaceae Escherichia coli K-12 and Klebsiella aerogenes appeared to be excellent feed to all three amoebae. Hardly any gro...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.59.7.2317-2319.1993
更新日期:1993-07-01 00:00:00
abstract::Carnobacterium maltaromaticum UAL307, isolated from fresh pork, exhibits potent activity against a number of gram-positive organisms, including numerous Listeria species. Three bacteriocins were isolated from culture supernatant, and using matrix-assisted laser desorption ionization-time of flight mass spectrometry an...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.00817-08
更新日期:2008-08-01 00:00:00
abstract::Sulfidogenic consortia enriched from an estuarine sediment were maintained on either 2-, 3-, or 4-chlorophenol as the only source of carbon and energy for over 5 years. The enrichment culture on 4-chlorophenol was the most active and this consortium was selected for further characterization. Utilization of chloropheno...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.61.4.1546-1550.1995
更新日期:1995-04-01 00:00:00
abstract::A variety of lactic acid bacteria, isolated from plant surfaces and plant-associated products, were found to be antagonistic to test strains of the phytopathogens Xanthomonas campestris, Erwinia carotovora, and Pseudomonas syringae. Effective "in vitro" inhibition was found both on agar plates and in broth cultures. I...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.52.3.552-555.1986
更新日期:1986-09-01 00:00:00
abstract::A microbial community analysis using 16S rRNA gene sequencing was performed on borehole water and a granite rock core from Henderson Mine, a >1,000-meter-deep molybdenum mine near Empire, CO. Chemical analysis of borehole water at two separate depths (1,044 m and 1,004 m below the mine entrance) suggests that a sharp ...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.01133-07
更新日期:2008-01-01 00:00:00
abstract::In a field experiment, Pseudomonas cepacia J82rif and J51rif increased sunflower emergence in the presence of the fungus Sclerotinia sclerotiorum. Pyrrolnitrin, aminopyrrolnitrin, and monochloroaminopyrrolnitrin were isolated from J82 and identified by using thin-layer chromatography, high-performance liquid chromatog...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.58.5.1760-1763.1992
更新日期:1992-05-01 00:00:00
abstract::ATP modulates immune cell functions, and ATP derived from gut commensal bacteria promotes the differentiation of T helper 17 (Th17) cells in the intestinal lamina propria. We recently reported that Enterococcus gallinarum, isolated from mice and humans, secretes ATP. We have since found and characterized several ATP-s...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.03871-12
更新日期:2013-04-01 00:00:00
abstract::The 3-hydroxy fatty acids (3-OHFAs) in lipopolysaccharides (LPS) play an important role in determining endotoxin activity, and childhood exposure to endotoxin has recently been associated with reduced risk of atopic diseases. To characterize the 3-OHFAs in house dust (HD), we used gas chromatography-mass spectrometry ...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/aem.70.1.262-267.2004
更新日期:2004-01-01 00:00:00
abstract::Glucose metabolism and the mechanisms of NADH oxidation by Treponema hyodysenteriae were studied. Under an N2 atmosphere, washed cell suspensions of the spirochete consumed glucose and produced acetate, butyrate, H2, and CO2. Approximately twice as much H2 as CO2 was produced. Determinations of radioactivity in produc...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.55.9.2365-2371.1989
更新日期:1989-09-01 00:00:00
abstract::The interaction of the peptide antibiotic nisin with liposomes has been studied. The effect of this interaction was analyzed on the membrane potential (inside negative) and the pH gradient (inside alkaline) in liposomes made from Escherichia coli phosphatidylethanolamine and egg phosphatidylcholine (9:1, wt/wt). The m...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.57.8.2164-2170.1991
更新日期:1991-08-01 00:00:00
abstract::Tropomyosin receptor kinase A (TrkA), a receptor tyrosine kinase, is known to be associated with various diseases. Thus, TrkA has become a major drug-screening target for these diseases. Despite the fact that the production of recombinant proteins by prokaryotic hosts has advantages, such as fast growth and ease of ge...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.03112-14
更新日期:2015-02-01 00:00:00
abstract::Thirty-three standard mycotoxins were assayed by thin-layer chromatography and by cytotoxicity in HEp-2 and Chang cells. Various levels of detection were found. The cytotoxicity test was significantly more sensitive than thin-layer chromatography for the trichothecenes and should be useful for screening extracts from ...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.46.4.948-950.1983
更新日期:1983-10-01 00:00:00
abstract::Seven organotin compounds and tin chloride were tested for their effects on the methanogenic bacteria Methanococcus thermolithotrophicus, Methanococcus deltae delta LH, and Methanosarcina barkeri 227. The methanogens were strongly inhibited by triethyltin, tripropyltin, and monophenyltin compounds, generally at concen...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.57.4.1189-1193.1991
更新日期:1991-04-01 00:00:00
abstract::Twenty known penicillic acid (PA)-producing Aspergillus and Penicillium cultures were grown under various conditions in shaken flasks to determine the highest yielding strains and their requirements for maximum toxin production. Abilities of the cultures to utilize eight different carbon sources in Raulin-Thom medium ...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.34.5.553-556.1977
更新日期:1977-11-01 00:00:00
abstract::There is growing evidence that anthropogenic sources of antibiotics and antimicrobial-resistant bacteria can spill over into natural ecosystems, raising questions about the role wild animals play in the emergence, maintenance, and dispersal of antibiotic resistance genes. In particular, we lack an understanding of how...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.02136-18
更新日期:2018-12-13 00:00:00
abstract::The metabolic and energetic characterization of the growth of Leuconostoc oenos on glucose-citrate or glucose-fructose mixtures enables the potential role of this bacterium in the wine-making process to be ascertained. Moreover, mixotrophic conditions remain a suitable means for improving biomass productivities of mal...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.60.5.1459-1466.1994
更新日期:1994-05-01 00:00:00
abstract::The effects of different organic substrates on the abilities of anaerobic sediment enrichments to reductively dechlorinate polychlorinated biphenyls (PCBs) were studied. Sediments collected from a site previously contaminated with PCBs were dosed with additional PCBs (Aroclor 1242; approximately 300 ppm [300 mug/g], s...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.56.9.2612-2617.1990
更新日期:1990-09-01 00:00:00
abstract::FK506 is an important 23-member polyketide macrolide with immunosuppressant activity. Its entire biosynthetic gene cluster was previously cloned from Streptomyces sp. strain KCTC 11604BP, and sequence analysis identified three putative regulatory genes, tcs2, tcs7, and fkbN, which encode proteins with high similarity ...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.06766-11
更新日期:2012-04-01 00:00:00
abstract::The gene encoding a novel 5-oxoprolinase without ATP-hydrolyzing activity from Alcaligenes faecalis N-38A was cloned and characterized. The coding region of this gene is 1,299 bp long. The predicted primary protein is composed of 433 amino acid residues, with a 31-amino-acid signal peptide. The mature protein is compo...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/aem.66.8.3201-3205.2000
更新日期:2000-08-01 00:00:00
abstract::To secure food and water safety, quantitative information on multiple pathogens is important. In this study, we developed a microfluidic quantitative PCR (MFQPCR) system to simultaneously quantify 11 major human viral pathogens, including adenovirus, Aichi virus, astrovirus, enterovirus, human norovirus, rotavirus, sa...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.02578-14
更新日期:2014-12-01 00:00:00
abstract::Pyrobaculum islandicum uses iron, thiosulfate, and elemental sulfur for anaerobic respiration, while Pyrobaculum aerophilum uses iron and nitrate; however, the constraints on these processes and their physiological mechanisms for iron and sulfur reduction are not well understood. Growth rates on sulfur compounds are h...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.02033-07
更新日期:2008-01-01 00:00:00
abstract::Pediocin PA-1 is an antimicrobial peptide (called bacteriocin) that shows inhibitory activity against the food-borne pathogen Listeria monocytogenes. To elucidate which residue(s) is responsible for this function, the antimicrobial activities of pediocin PA-1 mutants were evaluated and compared. Each of the 44 native ...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.72.2.1141-1147.2006
更新日期:2006-02-01 00:00:00
abstract::The known archaeal family B DNA polymerases are unable to participate in the PCR in the presence of uracil. Here, we report on a novel archaeal family B DNA polymerase from Nanoarchaeum equitans that can successfully utilize deaminated bases such as uracil and hypoxanthine and on its application to PCR. N. equitans fa...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.00624-08
更新日期:2008-11-01 00:00:00
abstract::Pseudomonas fluorescens Pf-5 is a soil bacterium that suppresses plant pathogens due in part to its production of the antibiotic pyoluteorin. Previous characterization of Pf-5 revealed three global regulators, including the stationary-phase sigma factor sigma(S) and the two-component regulators GacA and GacS, that inf...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/aem.66.7.2718-2725.2000
更新日期:2000-07-01 00:00:00
abstract::Six laboratories actively involved in water virology research participated in a methods evaluation study, conducted under the auspices of the American Society for Testing and Materials Committee on Viruses in the Aquatic Environment, Task Force on Drinking Water. Each participant was asked to examine the Viradel (viru...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.47.1.144-150.1984
更新日期:1984-01-01 00:00:00
abstract::Aerobic anoxygenic phototrophic (AAP) bacteria are photoheterotrophic microbes that are found in a broad range of aquatic environments. Although potentially significant to the microbial ecology and biogeochemistry of marine ecosystems, their abundance and genetic diversity and the environmental variables that regulate...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.06268-11
更新日期:2012-04-01 00:00:00