Functional expression of full-length TrkA in the prokaryotic host Magnetospirillum magneticum AMB-1 by using a magnetosome display system.

Abstract:

:Tropomyosin receptor kinase A (TrkA), a receptor tyrosine kinase, is known to be associated with various diseases. Thus, TrkA has become a major drug-screening target for these diseases. Despite the fact that the production of recombinant proteins by prokaryotic hosts has advantages, such as fast growth and ease of genetic engineering, the efficient production of functional receptor tyrosine kinase by prokaryotic hosts remains a major experimental challenge. Here, we report the functional expression of full-length TrkA on magnetosomes in Magnetospirillum magneticum AMB-1 by using a magnetosome display system. TrkAwas fused with the magnetosome-localized protein Mms13 and expressed on magnetosome surfaces. Recombinant TrkA showed both nerve growth factor (NGF)-binding and autophosphorylation activities. TrkA expressed on magnetosomes has the potential to be used, not only for further functional analysis of TrkA, but also for ligand screening.

journal_name

Appl Environ Microbiol

authors

Honda T,Yasuda T,Tanaka T,Hagiwara K,Arai T,Yoshino T

doi

10.1128/AEM.03112-14

subject

Has Abstract

pub_date

2015-02-01 00:00:00

pages

1472-6

issue

4

eissn

0099-2240

issn

1098-5336

pii

AEM.03112-14

journal_volume

81

pub_type

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