Visualization and enumeration of bacteria carrying a specific gene sequence by in situ rolling circle amplification.

abstract：:Toxigenic Vibrio cholerae, the causative agent of the epidemic diarrheal disease cholera, interacts with diverse environmental bacteriophages. These interactions promote genetic diversity or cause selective enrichment of phage-resistant bacterial clones. To identify bacterial genes involved in mediating the phage-resi...

journal_title：Applied and environmental microbiology

authors： Zahid MS,Waise TM,Kamruzzaman M,Ghosh AN,Nair GB,Mekalanos JJ,Faruque SM

更新日期：2010-07-01 00:00:00

abstract：:Biological dinitrogen fixation in mangrove communities of the Tampa Bay region of South Florida was investigated using the acetylene reduction technique. Low rates of acetylene reduction (0.01 to 1.84 nmol of C(2)H(4)/g [wet weight] per h) were associated with plant-free sediments, while plant-associated sediments gav...

journal_title：Applied and environmental microbiology

authors： Zuberer DA,Silver WS

更新日期：1978-03-01 00:00:00

abstract：:rRNA genetic markers were analyzed in 97 isolates of nodule bacteria from six legume species in Chihuahua, Mexico. The most common genotypes were widely shared across host species and had 16S rRNA sequences identical to those of strains from an eastern North American legume (Amphicarpaea) that are closely related to B...

journal_title：Applied and environmental microbiology

authors： Parker MA

更新日期：2002-04-01 00:00:00

abstract：:We found that the human intestinal isolate Bifidobacterium bifidum MIMBb75 strongly adhered to Caco-2 cells. Proteinase K and lithium chloride treatments showed that proteins play a key role in MIMBb75 adhesion to Caco-2 cells. By studying the cell wall-associated proteins, we identified a surface protein, which we la...

journal_title：Applied and environmental microbiology

authors： Guglielmetti S,Tamagnini I,Mora D,Minuzzo M,Scarafoni A,Arioli S,Hellman J,Karp M,Parini C

更新日期：2008-08-01 00:00:00

abstract：:Competitive exclusion of Salmonella enterica serovar Enteritidis by a mixed culture of Lactobacillus crispatus and Clostridium lactatifermentans was studied in a sequencing fed-batch reactor mimicking the cecal ecophysiology of broiler chickens. Growth of serovar Enteritidis was inhibited by a mixed culture of L. cris...

journal_title：Applied and environmental microbiology

authors： van der Wielen PW,Lipman LJ,van Knapen F,Biesterveld S

更新日期：2002-02-01 00:00:00

abstract：:In the luminal contents of metronidazole-treated rats, there was a dominant Bifidobacterium species. A strain has been isolated, its 16S rRNA gene has been sequenced, and the strain has been named Bifidobacterium pseudolongum strain Patronus. In this study, using an experimental model of healthy rats, the effects of m...

journal_title：Applied and environmental microbiology

authors： Vasquez N,Suau A,Magne F,Pochart P,Pélissier MA

更新日期：2009-01-01 00:00:00

abstract：:Sphingomonas paucimobilis B90A is able to degrade the alpha-, beta-, gamma-, and delta-isomers of hexachlorocyclohexane (HCH). It contains the genes linA, linB, linC, linD, linE, and linR, which have been implicated in HCH degradation. In this study, dynamic expression of the lin genes was measured in chemostat-grown ...

journal_title：Applied and environmental microbiology

authors： Suar M,van der Meer JR,Lawlor K,Holliger C,Lal R

更新日期：2004-11-01 00:00:00

abstract：:A protocol was developed to detect bacteria inhabiting microarthropods by means of small-subunit rRNA-targeted fluorescence in situ hybridization and microscopy. The protocol is based on cryosections of whole specimens. In contrast to more commonly applied paraffin-embedding techniques, the protocol is quicker and red...

journal_title：Applied and environmental microbiology

authors： Thimm T,Tebbe CC

更新日期：2003-05-01 00:00:00

abstract：:In our previous study, Bacillus subtilis strain BSK3S, containing a polymyxin biosynthetic gene cluster from Paenibacillus polymyxa, could produce polymyxin only in the presence of exogenously added L-2,4-diaminobutyric acid (Dab). The dependence of polymyxin production on exogenous Dab was removed by introducing an e...

journal_title：Applied and environmental microbiology

authors： Park SY,Choi SK,Kim J,Oh TK,Park SH

更新日期：2012-06-01 00:00:00

abstract：:The uncultivated bacterial endosymbionts of the hydrothermal vent tubeworm Riftia pachyptila play a central role in providing their host with fixed carbon. While this intimate association between host and symbiont indicates tight integration and coordination of function via cellular communication mechanisms, no such s...

journal_title：Applied and environmental microbiology

authors： Hughes DS,Felbeck H,Stein JL

更新日期：1997-09-01 00:00:00

abstract：:The consumption of acetate in soils taken from a nutrient gradient in the northern Florida Everglades was studied by stable isotope probing. Bacterial and archaeal 16S rRNA gene clone libraries from eutrophic and oligotrophic soil microcosms strongly suggest that a significant amount of acetate is consumed by syntroph...

journal_title：Applied and environmental microbiology

authors： Chauhan A,Ogram A

更新日期：2006-10-01 00:00:00

abstract：:During incubation of seawater in bottles, the decrease in dissolved oxygen is often nonlinear over time scales frequently used to measure respiration. Numbers of bacteria always increase, and rates of assimilation of dissolved leucine often increase exponentially. This suggests that sample handling disrupts the previo...

journal_title：Applied and environmental microbiology

authors： Pomeroy LR,Sheldon JE,Sheldon WM

更新日期：1994-01-01 00:00:00

abstract：:In this study, we fused six self-assembling amphipathic peptides (SAPs) with cyclodextrin glycosyltransferase (CGTase) from Paenibacillus macerans to catalyze 2-O-D-glucopyranosyl-L-ascorbic acid (AA-2G) production with cheap substrates, including maltose, maltodextrin, and soluble starch as glycosyl donors. The resul...

journal_title：Applied and environmental microbiology

authors： Han R,Li J,Shin HD,Chen RR,Liu L,Du G,Chen J

更新日期：2014-08-01 00:00:00

abstract：:Vinyl chloride reductases (VC-RDase) are the key enzymes for complete microbial reductive dehalogenation of chloroethenes, including the groundwater pollutants tetrachloroethene and trichloroethene. Analysis of the codon usage of the VC-RDase genes vcrA and bvcA showed that these genes are highly unusual and are chara...

journal_title：Applied and environmental microbiology

authors： McMurdie PJ,Behrens SF,Holmes S,Spormann AM

更新日期：2007-04-01 00:00:00

abstract：:Plasmodium falciparum, in addition to scavenging essential fatty acids from its intra- and intercellular environments, possesses a functional complement of type II fatty acid synthase (FAS) enzymes targeted to the apicoplast organelle. Recent evidence suggests that products of the plasmodial FAS II system may be criti...

journal_title：Applied and environmental microbiology

authors： Du Y,Gisselberg JE,Johnson JD,Lee PJ,Prigge ST,Bachmann BO

更新日期：2010-06-01 00:00:00

abstract：:Leptospirosis, an emerging zoonotic disease, remains poorly understood because of a lack of genetic manipulation tools available for pathogenic leptospires. Current genetic manipulation techniques include insertion of DNA by random transposon mutagenesis and homologous recombination via suicide vectors. This study des...

journal_title：Applied and environmental microbiology

authors： Pappas CJ,Benaroudj N,Picardeau M

更新日期：2015-05-01 00:00:00

abstract：:We recently characterized a transposon-induced NaCl-sensitive mutant of Staphylococcus aureus (U. Vijaranakul, M. J. Nadakavukaren, D. O. Bayles, B. J. Wilkinson, and R. K. Jayaswal, Appl. Environ. Microbiol. 63:1889-1897, 1997). To further characterize this mutant, we determined the nucleotide sequence at the inserti...

journal_title：Applied and environmental microbiology

authors： Vijaranakul U,Xiong A,Lockwood K,Jayaswal RK

更新日期：1998-02-01 00:00:00

abstract：:Amino acid modifications of the Thermobifida fusca Cel9A-68 catalytic domain or carbohydrate binding module 3c (CBM3c) were combined to create enzymes with changed amino acids in both domains. Bacterial crystalline cellulose (BC) and swollen cellulose (SWC) assays of the expressed and purified enzymes showed that thre...

journal_title：Applied and environmental microbiology

authors： Li Y,Irwin DC,Wilson DB

更新日期：2010-04-01 00:00:00

abstract：:Bacteria in the phylum Acidobacteria are widely distributed and abundant in soils, but their ecological roles are poorly understood, owing in part to a paucity of cultured representatives. In a molecular survey of acidobacterial diversity at the Michigan State University Kellogg Biological Station Long-Term Ecological...

journal_title：Applied and environmental microbiology

authors： Eichorst SA,Breznak JA,Schmidt TM

更新日期：2007-04-01 00:00:00

abstract：:A molecular method based on restriction fragment length polymorphism (RFLP) of PCR-amplified fragments of the 23S rRNA gene was designed to rapidly identify Listeria strains to the species level. Two fragments (S1, 460 bp, and S2, 890 bp) were amplified from boiled DNA. S2 was cut with the restriction enzymes XmnI or ...

journal_title：Applied and environmental microbiology

authors： Paillard D,Dubois V,Duran R,Nathier F,Guittet C,Caumette P,Quentin C

更新日期：2003-11-01 00:00:00

abstract：:Ruminal fluid from sheep fed hay plus concentrate diets containing 1.8% urea, 6% casein, or 6% egg albumin had proteolytic activities of 4.12, 3.02, or 4.00 mg of [14C]casein hydrolyzed ml-1 h-1, respectively. Dietary albumin had no effect on the rate of albumin breakdown relative to that of casein (0.06). Greater num...

journal_title：Applied and environmental microbiology

authors： Wallace RJ,Broderick GA,Brammall ML

更新日期：1987-04-01 00:00:00

abstract：:Pathogens may reach agricultural soils through application of animal manure and thereby pose a risk of contaminating crops as well as surface and groundwater. Treatment and handling of manure for improved nutrient and odor management may also influence the amount and fate of manure-borne pathogens in the soil. A study...

journal_title：Applied and environmental microbiology

authors： Amin MG,Forslund A,Bui XT,Juhler RK,Petersen SO,Lægdsmand M

更新日期：2013-01-01 00:00:00

abstract：:Antibodies to methanol dehydrogenase purified from Methylobacterium sp. strain AM1 and Methylomonas sp. strain A4 were raised. The antibody preparations were used in indirect immunogold labeling studies. With this approach, methanol dehydrogenase was found to be preferentially localized to the periplasmic region of th...

journal_title：Applied and environmental microbiology

authors： Fassel TA,Buchholz LA,Collins ML,Remsen CC

更新日期：1992-07-01 00:00:00

abstract：:We analyzed changes in bacterioplankton morphology and composition during enhanced protozoan grazing by image analysis and fluorescent in situ hybridization with group-specific rRNA-targeted oligonucleotide probes. Enclosure experiments were conducted in a small, fishless freshwater pond which was dominated by the cla...

journal_title：Applied and environmental microbiology

authors： Jürgens K,Pernthaler J,Schalla S,Amann R

更新日期：1999-03-01 00:00:00

abstract：:Lyophilizatin of 13 cyanobacterial cultures belonging to seven genera was attempted in a variety of suspending substances. All organisms survived lyophilization when suspended in lamb serum. Some of the organisms could be successfully lyophilized in horse serum, beef serum, fetal bovine serum, or human ascites fluid. ...

journal_title：Applied and environmental microbiology

authors： Corbett LL,Parker DL

更新日期：1976-12-01 00:00:00

abstract：:Obligate aerobic organisms rely on a functional electron transport chain for energy conservation and NADH oxidation. Because of this essential requirement, the genes of this pathway are likely constitutively and highly expressed to avoid a cofactor imbalance and energy shortage under fluctuating environmental conditio...

journal_title：Applied and environmental microbiology

authors： Nies SC,Dinger R,Chen Y,Wordofa GG,Kristensen M,Schneider K,Büchs J,Petzold CJ,Keasling JD,Blank LM,Ebert BE

更新日期：2020-05-19 00:00:00

abstract：:Microwave radiation in Escherichia coli and Bacillus subtilis cell suspensions resulted in a dramatic reduction of the viable counts as well as increases in the amounts of DNA and protein released from the cells according to the increase of the final temperature of the cell suspensions. However, no significant reducti...

journal_title：Applied and environmental microbiology

authors： Woo IS,Rhee IK,Park HD

更新日期：2000-05-01 00:00:00

abstract：:Mineral soil-based inoculants of Rhizobium meliloti and Rhizobium phaseoli survived better at 4 degrees C than at higher temperatures, but ca. 15% of the cells were viable at 37 degrees C after 27 days. Soil-based inoculants of R. meliloti, R. phaseoli, Rhizobium japonicum, and a cowpea Rhizobium sp. applied to seeds ...

journal_title：Applied and environmental microbiology

authors： Chao WL,Alexander M

更新日期：1984-01-01 00:00:00

abstract：:Comparative analysis of the 16S rRNA gene and fluorescent in situ hybridization (FISH) was used to identify epibiotic filamentous bacteria living on the marine amphipod crustacean Urothoe poseidonis. The epibionts belong to the gamma proteobacteria and represent a novel marine phylotype within the genus Thiothrix. FIS...

journal_title：Applied and environmental microbiology

authors： Gillan DC,Dubilier N

更新日期：2004-06-01 00:00:00

abstract：:The temperature-driven adaptation of the bacterial community in peat was studied, by altering temperature to simulate self-heating and a subsequent return to mesophilic conditions. The technique used consisted of extracting the bacterial community from peat using homogenization-centrifugation and measuring the rates o...

journal_title：Applied and environmental microbiology

authors： Ranneklev SB,Bååth E

更新日期：2001-03-01 00:00:00