Abstract:
:Amino acid modifications of the Thermobifida fusca Cel9A-68 catalytic domain or carbohydrate binding module 3c (CBM3c) were combined to create enzymes with changed amino acids in both domains. Bacterial crystalline cellulose (BC) and swollen cellulose (SWC) assays of the expressed and purified enzymes showed that three combinations resulted in 150% and 200% increased activity, respectively, and also increased synergistic activity with other cellulases. Several other combinations resulted in drastically lowered activity, giving insight into the need for a balance between the binding in the catalytic cleft on either side of the cleavage site, as well as coordination between binding affinity for the catalytic domain and CBM3c. The same combinations of amino acid variants in the whole enzyme, Cel9A-90, did not increase BC or SWC activity but did have higher filter paper (FP) activity at 12% digestion.
journal_name
Appl Environ Microbioljournal_title
Applied and environmental microbiologyauthors
Li Y,Irwin DC,Wilson DBdoi
10.1128/AEM.02735-09subject
Has Abstractpub_date
2010-04-01 00:00:00pages
2582-8issue
8eissn
0099-2240issn
1098-5336pii
AEM.02735-09journal_volume
76pub_type
杂志文章abstract::To enhance the production of isoprene, a volatile 5-carbon hydrocarbon, in the Gram-positive spore-forming rod-shaped bacterium Bacillus subtilis, 1-deoxy-d-xylulose-5-phosphate synthase (Dxs) and 1-deoxy-d-xylulose-5-phosphate reductoisomerase (Dxr) were overexpressed in B. subtilis DSM 10. For the strain that overex...
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pub_type: 杂志文章,随机对照试验
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journal_title:Applied and environmental microbiology
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pub_type: 杂志文章,meta分析
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journal_title:Applied and environmental microbiology
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journal_title:Applied and environmental microbiology
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doi:10.1128/AEM.45.6.1761-1766.1983
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journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.56.5.1504-1508.1990
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journal_title:Applied and environmental microbiology
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journal_title:Applied and environmental microbiology
pub_type: 杂志文章
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journal_title:Applied and environmental microbiology
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journal_title:Applied and environmental microbiology
pub_type: 杂志文章
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更新日期:1999-04-01 00:00:00