Enhancing isoprene production by genetic modification of the 1-deoxy-d-xylulose-5-phosphate pathway in Bacillus subtilis.

abstract：:Double-stranded RNA (dsRNA) viruses in some fungi are associated with hypovirulence and have been used or proposed as biological control agents. We isolated 7.5-kb dsRNAs from 13 of 286 field strains of Fusarium graminearum isolated from maize in Korea. One of these strains, DK21, was examined in more detail. This str...

journal_title：Applied and environmental microbiology

authors： Chu YM,Jeon JJ,Yea SJ,Kim YH,Yun SH,Lee YW,Kim KH

更新日期：2002-05-01 00:00:00

abstract：:A gene encoding a new thermostable D-stereospecific alanine amidase from the thermophile Brevibacillus borstelensis BCS-1 was cloned and sequenced. The molecular mass of the purified enzyme was estimated to be 199 kDa after gel filtration chromatography and about 30 kDa on sodium dodecyl sulfate-polyacrylamide gel ele...

journal_title：Applied and environmental microbiology

authors： Baek DH,Kwon SJ,Hong SP,Kwak MS,Lee MH,Song JJ,Lee SG,Yoon KH,Sung MH

更新日期：2003-02-01 00:00:00

abstract：:Using a chemically defined medium without L-alanine, Lactobacillus johnsonii was demonstrated to be strictly auxotrophic for that amino acid. A comparative genetic analysis showed that all known genes involved in L-alanine biosynthesis are absent from the genome of L. johnsonii. This auxotrophy was complemented by het...

journal_title：Applied and environmental microbiology

authors： van der Kaaij H,Desiere F,Mollet B,Germond JE

更新日期：2004-03-01 00:00:00

abstract：:Anaerobic ammonium oxidation is a recent addition to the microbial nitrogen cycle, and its metabolic pathway, including the production and conversion of its intermediate hydrazine, is not well understood. Therefore, the effect of hydroxylamine addition on the hydrazine metabolism of anaerobic ammonium-oxidizing (anamm...

journal_title：Applied and environmental microbiology

authors： van der Star WR,van de Graaf MJ,Kartal B,Picioreanu C,Jetten MS,van Loosdrecht MC

更新日期：2008-07-01 00:00:00

abstract：:Arbitrarily primed polymerase chain reaction, with incorporation of either radioactive or fluorescent labels, was used as a rapid and sensitive method for obtaining genomic fingerprints of strains of Lactococcus lactis. Closely related strains produced almost identical fingerprints. Fingerprints of other strains showe...

journal_title：Applied and environmental microbiology

authors： Cancilla MR,Powell IB,Hillier AJ,Davidson BE

更新日期：1992-05-01 00:00:00

abstract：:Root-colonizing, saprophytic fluorescent pseudomonads of the Pseudomonas putida-P. fluorescens group express similar levels of catalase and superoxide dismutase activities during growth on a sucrose- and amino acid-rich medium. Increased specific activities of catalase but not superoxide dismutase were observed during...

journal_title：Applied and environmental microbiology

authors： Katsuwon J,Anderson AJ

更新日期：1990-11-01 00:00:00

abstract：:The developing intestinal microbiota of breast-fed infants is considered to play an important role in the priming of the infants' mucosal and systemic immunity. Generally, Bifidobacterium and Lactobacillus predominate the microbiota of breast-fed infants. In intervention trials it has been shown that lactobacilli can ...

journal_title：Applied and environmental microbiology

authors： Haarman M,Knol J

更新日期：2006-04-01 00:00:00

abstract：:Bacterial communities associated with marine algae are often dominated by members of the Roseobacter clade, and in the present study, we describe Roseobacter phenotypes that may provide this group of bacteria with selective advantages when colonizing this niche. Nine of 14 members of the Roseobacter clade, of which ha...

journal_title：Applied and environmental microbiology

authors： Bruhn JB,Gram L,Belas R

更新日期：2007-01-01 00:00:00

abstract：:The recent advent of tools enabling statistical inferences to be drawn from comparisons of microbial communities has enabled the focus of microbial ecology to move from characterizing biodiversity to describing the distribution of that biodiversity. Although statistical tools have been developed to compare community s...

journal_title：Applied and environmental microbiology

authors： Schloss PD,Handelsman J

更新日期：2006-10-01 00:00:00

abstract：:Silver toxicity is a problem that microorganisms face in medical and environmental settings. Through exposure to silver compounds, some bacteria have adapted to growth in high concentrations of silver ions. Such adapted microbes may be dangerous as pathogens but, alternatively, could be potentially useful in nanomater...

journal_title：Applied and environmental microbiology

authors： Sedlak RH,Hnilova M,Grosh C,Fong H,Baneyx F,Schwartz D,Sarikaya M,Tamerler C,Traxler B

更新日期：2012-04-01 00:00:00

abstract：:The abundance of quorum quenching (QQ) activity was evaluated in cultivable bacteria obtained from oceanic and estuarine seawater and compared with the frequency of QQ enzyme sequences in the available marine metagenomic collections. The possible role of the high QQ activity found among marine bacteria is discussed. ...

journal_title：Applied and environmental microbiology

authors： Romero M,Martin-Cuadrado AB,Otero A

更新日期：2012-09-01 00:00:00

abstract：:Pseudomonas aeruginosa exhibits flagellum-mediated swimming in liquid and swarming on hydrated surfaces under diverse nutrient conditions. Prior studies have implicated a phosphodiesterase, DipA, in regulating these flagellum-mediated motilities, but collectively, the necessity for DipA was unclear. In this study, we ...

journal_title：Applied and environmental microbiology

authors： Mattingly AE,Kamatkar NG,Morales-Soto N,Borlee BR,Shrout JD

更新日期：2018-03-19 00:00:00

abstract：:We have constructed the first Escherichia coli-Veillonella shuttle vector based on an endogenous plasmid (pVJL1) isolated from a clinical Veillonella strain. A highly transformable Veillonella strain was also identified. Both the shuttle vector and the transformable strain should be valuable tools for future Veillonel...

journal_title：Applied and environmental microbiology

authors： Liu J,Xie Z,Merritt J,Qi F

更新日期：2012-05-01 00:00:00

abstract：:Parasitic chytrid fungi can inflict significant mortality on cyanobacteria but frequently fail to keep cyanobacterial dominance and bloom formation in check. Our study tested whether oligopeptide production, a common feature in many cyanobacteria, can be a defensive mechanism against chytrid parasitism. The study empl...

journal_title：Applied and environmental microbiology

authors： Rohrlack T,Christiansen G,Kurmayer R

更新日期：2013-04-01 00:00:00

abstract：:Bacteriophage resistance mechanisms which are derived from a bacteriophage genome are termed Per (phage-encoded resistance). When present in trans in Lactococcus lactis NCK203, Per50, the cloned origin of replication from phage phi50, interferes with phi50 replication. The per50 fragment was found to afford negligible...

journal_title：Applied and environmental microbiology

authors： O'sullivan DJ,Hill C,Klaenhammer TR

更新日期：1993-08-01 00:00:00

abstract：:The genetic diversity of 138 strains of the Xanthomonas pathovar mangiferaeindicae, which were isolated from three different hosts (mango, ambarella, and pepper tree) in 14 different countries, was assessed with restriction fragment length polymorphism markers. An analysis of patterns obtained by hybridization with an...

journal_title：Applied and environmental microbiology

authors： Gagnevin L,Leach JE,Pruvost O

更新日期：1997-01-01 00:00:00

abstract：:Interactions between photoautotrophic and heterotrophic microorganisms are central to the marine microbial ecosystem. Lab cultures of one of the dominant marine photoautotrophs, Synechococcus, have historically been difficult to render axenic, presumably because these bacteria depend upon other organisms to grow under...

journal_title：Applied and environmental microbiology

authors： Zheng Q,Wang Y,Xie R,Lang AS,Liu Y,Lu J,Zhang X,Sun J,Suttle CA,Jiao N

更新日期：2018-01-17 00:00:00

abstract：:Contamination of soil and water with heavy metals and metalloids is a serious environmental problem. Cadmium and arsenic are major environmental contaminants that pose a serious threat to human health. Although toxicities of cadmium and arsenic to living organisms have been extensively studied, the molecular mechanism...

journal_title：Applied and environmental microbiology

authors： Techo T,Charoenpuntaweesin S,Auesukaree C

更新日期：2020-10-15 00:00:00

abstract：:Strain UHM-5, a pSym Exo derivative of the aluminum-tolerant Rhizobium leguminosarum bv. phaseoli strain CIAT899, was equally tolerant of aluminum (Al) as the parental culture. Dialyzed culture supernatants of the wild-type cells grown in YEM broth (10 cells ml) contained 185 mug of glucose equivalents ml whereas UHM-...

journal_title：Applied and environmental microbiology

authors： Kingsley MT,Bohlool BB

更新日期：1992-04-01 00:00:00

abstract：:The known archaeal family B DNA polymerases are unable to participate in the PCR in the presence of uracil. Here, we report on a novel archaeal family B DNA polymerase from Nanoarchaeum equitans that can successfully utilize deaminated bases such as uracil and hypoxanthine and on its application to PCR. N. equitans fa...

journal_title：Applied and environmental microbiology

authors： Choi JJ,Song JG,Nam KH,Lee JI,Bae H,Kim GA,Sun Y,Kwon ST

更新日期：2008-11-01 00:00:00

abstract：:Human beta-hexosaminidase A (HexA) is a heterodimeric glycoprotein composed of alpha- and beta-subunits that degrades GM2 gangliosides in lysosomes. GM2 gangliosidosis is a lysosomal storage disease in which an inherited deficiency of HexA causes the accumulation of GM2 gangliosides. In order to prepare a large amount...

journal_title：Applied and environmental microbiology

authors： Akeboshi H,Chiba Y,Kasahara Y,Takashiba M,Takaoka Y,Ohsawa M,Tajima Y,Kawashima I,Tsuji D,Itoh K,Sakuraba H,Jigami Y

更新日期：2007-08-01 00:00:00

abstract：:The presence of Yersinia ruckeri in a French fish farm was investigated. Y. ruckeri was isolated mainly from algae and sediment samples rather than from water. Twenty-two Y. ruckeri isolates were obtained, and three strains were distinguished by enterobacterial repetitive intergenic consensus PCR amplification. These ...

journal_title：Applied and environmental microbiology

authors： Coquet L,Cosette P,Quillet L,Petit F,Junter GA,Jouenne T

更新日期：2002-02-01 00:00:00

abstract：:The respiratory and photosynthetic quinones of microbial mats which occurred in Japanese sulfide-containing neutral-pH hot springs at different temperatures were analyzed by spectrochromatography and mass spectrometry. All of the microbial mats that developed at high temperatures (temperatures above 68 degreesC) were ...

journal_title：Applied and environmental microbiology

authors： Hiraishi A,Umezawa T,Yamamoto H,Kato K,Maki Y

更新日期：1999-01-01 00:00:00

abstract：:Vegetative mycelia and spores of the investigated high- and low-producer strains of Streptomyces griseus bound significant amounts (4%) of streptomycin, which could be removed by increasing ionic strength. The release of antibiotic from the spores was easier when the spores were germinating. This phenomenon is conside...

journal_title：Applied and environmental microbiology

authors： Szabó I,Benedek A,Barabás G

更新日期：1985-08-01 00:00:00

abstract：:The population dynamics of pseudomonads in gilt-head sea bream Mediterranean fish (Sparus aurata) stored under different conditions were studied. Phenotypic analysis and sodium dodecyl sulfate-polyacrylamide gel electrophoresis of whole-cell proteins were performed to identify a total of 106 Pseudomonas strains isolat...

journal_title：Applied and environmental microbiology

authors： Tryfinopoulou P,Tsakalidou E,Nychas GJ

更新日期：2002-01-01 00:00:00

abstract：:The bacteriophage T4 denV gene encodes a well-characterized DNA repair enzyme involved in pyrimidine photodimer excision. We have discovered the first homologs of the denV gene in chlorella viruses, which are common in fresh water. This gene functions in vivo and also when cloned in Escherichia coli. Photodamaged viru...

journal_title：Applied and environmental microbiology

authors： Furuta M,Schrader JO,Schrader HS,Kokjohn TA,Nyaga S,McCullough AK,Lloyd RS,Burbank DE,Landstein D,Lane L,Van Etten JL

更新日期：1997-04-01 00:00:00

abstract：:Leghorn hens over 50 weeks of age were assigned to two treatment groups designated as either unmolted controls or molted. A forced molt was induced by a 9-day feed withdrawal, and each hen was challenged orally with 10(5) Salmonella enteritidis organisms on day 4 of feed withdrawal. On days 4 and 9 of molt, the number...

journal_title：Applied and environmental microbiology

authors： Durant JA,Corrier DE,Byrd JA,Stanker LH,Ricke SC

更新日期：1999-05-01 00:00:00

abstract：:The ability of Streptococcus species to selectively adhere to the oral epithelial cells of newborns was studied in vitro. On day 1 of life, mucosal cells from normal infants demonstrated selective attraction for the natural distribution of streptococci that would soon colonize these surfaces. Streptococcus salivarius ...

journal_title：Applied and environmental microbiology

authors： Long SS,Swenson RM

更新日期：1976-10-01 00:00:00

abstract：:Microbial communities in cores obtained from methane hydrate-bearing deep marine sediments (down to more than 300 m below the seafloor) in the forearc basin of the Nankai Trough near Japan were characterized with cultivation-dependent and -independent techniques. Acridine orange direct count data indicated that cell n...

journal_title：Applied and environmental microbiology

authors： Reed DW,Fujita Y,Delwiche ME,Blackwelder DB,Sheridan PP,Uchida T,Colwell FS

更新日期：2002-08-01 00:00:00

abstract：:Nested PCR with three highly degenerate primers was used for amplification and identification of DNA polymerase (pol) genes from viruses which infect three genera of microalgae. Group-specific primers (AVS1 and AVS2) were designed on the basis of inferred amino acid sequences unique to the DNA pol genes of viruses (PB...

journal_title：Applied and environmental microbiology

authors： Chen F,Suttle CA

更新日期：1995-04-01 00:00:00