Cyclization reaction catalyzed by glycogen debranching enzyme (EC 2.4.1.25/EC 3.2.1.33) and its potential for cycloamylose production.

Abstract:

:Glycogen debranching enzyme (GDE) has 4-alpha-glucanotransferase and amylo-1,6-glucosidase activities in the single polypeptide chain. We analyzed the detailed action profile of GDE from Saccharomyces cerevisiae on amylose and tested whether GDE catalyzes cyclization of amylose. GDE treatment resulted in a rapid reduction of absorbance of iodine-amylose complex and the accumulation of a product that was resistant to an exo-amylase (glucoamylase [GA]) but was degraded by an endo-type alpha-amylase to glucose and maltose. These results indicated that GDE catalyzed cyclization of amylose to produce cyclic alpha-1,4 glucan (cycloamylose). The formation of cycloamylose was confirmed by high-performance anion-exchange chromatography, and the size was shown to range from a degree of polymerization of 11 to a degree of polymerization around 50. The minimum size and the size distribution of cycloamylose were different from those of cycloamylose produced by other 4-alpha-glucanotransferases. GDE also efficiently produced cycloamylose even from the branched glucan substrate, starch, demonstrating its potential for industrial production of cycloamylose.

journal_name

Appl Environ Microbiol

authors

Yanase M,Takata H,Takaha T,Kuriki T,Smith SM,Okada S

doi

10.1128/aem.68.9.4233-4239.2002

keywords:

subject

Has Abstract

pub_date

2002-09-01 00:00:00

pages

4233-9

issue

9

eissn

0099-2240

issn

1098-5336

journal_volume

68

pub_type

杂志文章
  • Characterization of biphenyl dioxygenase sequences and activities encoded by the metagenomes of highly polychlorobiphenyl-contaminated soils.

    abstract::Total extracted DNA from two heavily polychlorobiphenyl-contaminated soils was analyzed with respect to biphenyl dioxygenase sequences and activities. This was done by PCR amplification and cloning of a DNA segment encoding the active site of the enzyme. The translated sequences obtained fell into three similarity clu...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.07381-11

    authors: Standfuss-Gabisch C,Al-Halbouni D,Hofer B

    更新日期:2012-04-01 00:00:00

  • Genomic potential of Marinobacter aquaeolei, a biogeochemical "opportunitroph".

    abstract::The genus of Marinobacter is one of the most ubiquitous in the global oceans and assumed to significantly impact various biogeochemical cycles. The genome structure and content of Marinobacter aquaeolei VT8 was analyzed and compared with those from other organisms with diverse adaptive strategies. Here, we report the ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.01866-10

    authors: Singer E,Webb EA,Nelson WC,Heidelberg JF,Ivanova N,Pati A,Edwards KJ

    更新日期:2011-04-01 00:00:00

  • Molybdenum Availability Is Key to Nitrate Removal in Contaminated Groundwater Environments.

    abstract::The concentrations of molybdenum (Mo) and 25 other metals were measured in groundwater samples from 80 wells on the Oak Ridge Reservation (ORR) (Oak Ridge, TN), many of which are contaminated with nitrate, as well as uranium and various other metals. The concentrations of nitrate and uranium were in the ranges of 0.1 ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.00917-15

    authors: Thorgersen MP,Lancaster WA,Vaccaro BJ,Poole FL,Rocha AM,Mehlhorn T,Pettenato A,Ray J,Waters RJ,Melnyk RA,Chakraborty R,Hazen TC,Deutschbauer AM,Arkin AP,Adams MW

    更新日期:2015-08-01 00:00:00

  • Antagonistic activity exerted in vitro and in vivo by Lactobacillus casei (strain GG) against Salmonella typhimurium C5 infection.

    abstract::The aim of this study was to compare the antagonistic properties of Lactobacillus casei GG exerted in vitro against Salmonella typhimurium C5 in a cellular model, cultured enterocyte-like Caco-2 cells, to those exerted in vivo in an animal model, C3H/He/Oujco mice. Our results show that a 1-h contact between the invad...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.63.2.513-518.1997

    authors: Hudault S,Liévin V,Bernet-Camard MF,Servin AL

    更新日期:1997-02-01 00:00:00

  • Evaluation of broiler litter with reference to the microbial composition as assessed by using 16S rRNA and functional gene markers.

    abstract::Very little is known about the microbial composition of animal bedding wastes, including poultry litter, and what is known has been deduced from standard culture methods, by which some fastidious organisms that exist in the environment may not be detected. We evaluated the bacterial composition of poultry litter by us...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/aem.69.2.901-908.2003

    authors: Lu J,Sanchez S,Hofacre C,Maurer JJ,Harmon BG,Lee MD

    更新日期:2003-02-01 00:00:00

  • Isolation and characterization of Micromonospora phage PhiHAU8 and development into a phasmid.

    abstract::PhiHAU8, a temperate Micromonospora phage, which is capable of infecting Micromonospora sp. strains 40027 and A-M-01, was isolated. The PhiHAU8 virion has a polyhedral head and a flexible tail and has a small genome (ca. 42.5 kb) with double-stranded DNA and cohesive ends. PhiHAU8 was most stable at 4 degrees C in Dif...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.70.7.3893-3897.2004

    authors: Li X,Zhou X,Deng Z

    更新日期:2004-07-01 00:00:00

  • Construction of an Escherichia coli-Clostridium perfringens shuttle vector and plasmid transformation of Clostridium perfringens.

    abstract::A stable shuttle vector which replicates in Escherichia coli and Clostridium perfringens was constructed by ligating a 3.6-kilobase (kb) fragment of plasmid pBR322 with C. perfringens plasmid pHB101 (3.1 kb). The marker for this shuttle plasmid originated from the 1.3-kb chloramphenicol resistance gene of plasmid pHR1...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.55.2.360-365.1989

    authors: Kim AY,Blaschek HP

    更新日期:1989-02-01 00:00:00

  • Role of host nutrition in symbiont regulation: impact of dietary nitrogen on proliferation of obligate and facultative bacterial endosymbionts of the pea aphid Acyrthosiphon pisum.

    abstract::The impact of host nutrition on symbiont regulation in the pea aphid Acyrthosiphon pisum was investigated. The population density of the obligate symbiont Buchnera aphidicola positively correlated with dietary nitrogen levels. In contrast, the population density of the facultative symbiont Serratia symbiotica increase...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.01211-06

    authors: Wilkinson TL,Koga R,Fukatsu T

    更新日期:2007-02-01 00:00:00

  • Characterization of a new pathway for epichlorohydrin degradation by whole cells of xanthobacter strain py2.

    abstract::The degradation of epichlorohydrin (3-chloropropylene oxide or 1-chloro-2,3-epoxypropane) by whole-cell suspensions of Xanthobacter strain Py2 was investigated. Cell suspensions prepared from cultures grown with propylene as the carbon source readily degraded epichlorohydrin. The ability to degrade epichlorohydrin cor...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.61.4.1507-1513.1995

    authors: Small FJ,Tilley JK,Ensign SA

    更新日期:1995-04-01 00:00:00

  • Molecular evidence for multiple infections as revealed by typing of Asaia bacterial symbionts of four mosquito species.

    abstract::The recent increased detection of acetic acid bacteria (AAB) of the genus Asaia as symbionts of mosquitoes, such as Anopheles spp. and Aedes spp., prompted us to investigate the diversity of these symbionts and their relationships in different mosquito species and populations. Following cultivation-dependent and -inde...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.01747-10

    authors: Chouaia B,Rossi P,Montagna M,Ricci I,Crotti E,Damiani C,Epis S,Faye I,Sagnon N,Alma A,Favia G,Daffonchio D,Bandi C

    更新日期:2010-11-01 00:00:00

  • Differential Expression of Virulence Genes and Motility in Ralstonia (Pseudomonas) solanacearum during Exponential Growth.

    abstract::A complex network regulates virulence in Ralstonia solanacearum (formerly Pseudomonas solanacearum); central to this system is PhcA, a LysR-type transcriptional regulator. We report here that two PhcA-regulated virulence factors, endoglucanase (Egl) and acidic exopolysaccharide I (EPS I), and motility are expressed di...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.63.3.844-850.1997

    authors: Clough SJ,Flavier AB,Schell MA,Denny TP

    更新日期:1997-03-01 00:00:00

  • The Effects of Soil Depth on the Structure of Microbial Communities in Agricultural Soils in Iowa, USA.

    abstract::This study investigated the differences in microbial community abundance, composition and diversity throughout the depth profiles in soils collected from corn and soybean fields in lowa, USA using 16S rRNA amplicon sequencing. The results revealed decreased richness and diversity in microbial communities at increasing...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.02673-20

    authors: Hao J,Chai YN,Lopes LD,Ordóñez RA,Wright EE,Archontoulis S,Schachtman DP

    更新日期:2020-12-11 00:00:00

  • Concentration and diversity of noroviruses detected in Luxembourg wastewaters in 2008-2009.

    abstract::Noroviruses (NoV) in 78 wastewater samples from Luxembourg were quantified, cloned, and sequenced in 2008-2009. The concentrations of NoV genogroup II and the relative occurrences of certain genotypes changed significantly during the winter season. NoV genogroup I was frequently detected by real-time reverse transcrip...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.00632-11

    authors: Skraber S,Langlet J,Kremer JR,Mossong J,De Landtsheer S,Even J,Muller CP,Hoffmann L,Cauchie HM

    更新日期:2011-08-01 00:00:00

  • Comparison of methods for DNA isolation from food samples for detection of Shiga toxin-producing Escherichia coli by real-time PCR.

    abstract::In this study, food samples were intentionally contaminated with Escherichia coli O157:H7, and then DNA was isolated by using four commercial kits. The isolated DNA samples were compared by using real-time PCR detection of the Shiga toxin genes. The four kits tested worked similarly. ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/aem.69.3.1844-1846.2003

    authors: Heller LC,Davis CR,Peak KK,Wingfield D,Cannons AC,Amuso PT,Cattani J

    更新日期:2003-03-01 00:00:00

  • Poultry-associated Salmonella enterica subsp. enterica serovar 4,12:d:- reveals high clonality and a distinct pathogenicity gene repertoire.

    abstract::A European baseline survey during the years 2005 and 2006 has revealed that the monophasic Salmonella enterica subsp. enterica serovar 4,12:d:- was, with a prevalence of 23.6%, the most frequently isolated serovar in German broiler flocks. In Denmark and the United Kingdom, its serovar prevalences were 15.15% and 2.8%...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.02187-08

    authors: Huehn S,Bunge C,Junker E,Helmuth R,Malorny B

    更新日期:2009-02-01 00:00:00

  • Prevalence of and associated risk factors for shedding Cryptosporidium parvum oocysts and Giardia cysts within feral pig populations in California.

    abstract::Populations of feral pigs (Sus scrofa) may serve as an environmental reservoir of Cryptosporidium parvum oocysts and Giardia sp. cysts for source water. We conducted a cross-sectional study to determine the prevalence of and associated demographic and environmental risk factors for the shedding of C. parvum oocysts an...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.63.10.3946-3949.1997

    authors: Atwill ER,Sweitzer RA,Pereira MG,Gardner IA,Van Vuren D,Boyce WM

    更新日期:1997-10-01 00:00:00

  • Nitrogen control of atrazine utilization in Pseudomonas sp. strain ADP.

    abstract::Pseudomonas sp. strain ADP uses the herbicide atrazine as the sole nitrogen source. We have devised a simple atrazine degradation assay to determine the effect of other nitrogen sources on the atrazine degradation pathway. The atrazine degradation rate was greatly decreased in cells grown on nitrogen sources that supp...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/aem.69.12.6987-6993.2003

    authors: García-González V,Govantes F,Shaw LJ,Burns RG,Santero E

    更新日期:2003-12-01 00:00:00

  • Adaptation of Denitrifying Populations to Low Soil pH.

    abstract::Natural denitrification rates and activities of denitrifying enzymes were measured in an agricultural soil which had a 20-year past history of low pH (pH ca. 4) due to fertilization with acid-generating ammonium salts. The soil adjacent to this site had been limed and had a pH of ca. 6.0. Natural denitrification rates...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.49.5.1053-1056.1985

    authors: Parkin TB,Sexstone AJ,Tiedje JM

    更新日期:1985-05-01 00:00:00

  • Expression of a Clostridium thermocellum endoglucanase gene in Lactobacillus plantarum.

    abstract::Recombinant plasmid pM25 containing the celE gene of Clostridium thermocellum, which codes for an enzymatically active endoglucanase, was transformed into Lactobacillus plantarum by electroporation. Strains harboring pM25 expressed thermostable endoglucanase, which was found predominantly in the culture medium. Two ot...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.55.8.2095-2097.1989

    authors: Bates EE,Gilbert HJ,Hazlewood GP,Huckle J,Laurie JI,Mann SP

    更新日期:1989-08-01 00:00:00

  • Odorous Sulfur Compounds Emitted during Production of Compost Used as a Substrate in Mushroom Cultivation.

    abstract::Large-scale composting facilities are known to cause environmental problems, mainly through pungent air emitted by composting material. In air samples taken above stacks set up to prepare compost used as a substrate in mushroom cultivation, several volatile compounds were identified by means of the coupled techniques ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.56.1.176-180.1990

    authors: Derikx PJ,Op Den Camp HJ,van der Drift C,van Griensven LJ,Vogels GD

    更新日期:1990-01-01 00:00:00

  • Lysogenic strains of lactic Acid streptococci and lytic spectra of their temperate bacteriophages.

    abstract::A total of 113 strains of mesophilic strains lactic streptococci of the species Streptococcus lactis, S. lactis subsp. diacetilactis, and S. cremoris, chosen from 291 strains that had been previously classified into six groups on the basis of their sensitivity to 132 virulent phages, were subjected to induction with m...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.43.2.349-356.1982

    authors: Reyrolle J,Chopin MC,Letellier F,Novel G

    更新日期:1982-02-01 00:00:00

  • Microbial Communities of Orange Tubercles in Accelerated Low-Water Corrosion.

    abstract::The rapid degradation of marine infrastructure at the low tide level due to accelerated low-water corrosion (ALWC) is a problem encountered worldwide. Despite this, there is limited understanding of the microbial communities involved in this process. We obtained samples of the orange-colored tubercles commonly associa...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.00610-20

    authors: Phan HC,Wade SA,Blackall LL

    更新日期:2020-06-17 00:00:00

  • Detoxification of corn antimicrobial compounds as the basis for isolating Fusarium verticillioides and some other Fusarium species from corn.

    abstract::The preformed antimicrobial compounds produced by maize, 2,4-dihydroxy-7-methoxy-2H-1,4-benzoxazin-3-one and its desmethoxy derivative 2,4-dihydroxy-2H-1,4-benzoxazin-3-one, are highly reactive benzoxazinoids that quickly degrade to the antimicrobials 6-methoxy-2-benzoxazolinone (MBOA) and 2-benzoxazolinone (BOA), res...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.67.7.2973-2981.2001

    authors: Glenn AE,Hinton DM,Yates IE,Bacon CW

    更新日期:2001-07-01 00:00:00

  • Deterioration-Associated Microbiome of Stone Monuments: Structure, Variation, and Assembly.

    abstract::Research on the microbial communities that colonize stone monuments may provide a new understanding of stone biodeterioration and microbe-induced carbonate precipitation. This work investigated the seasonal variation of microbial communities in 2016 and 2017, as well as its effects on stone monuments. We determined th...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.02680-17

    authors: Li Q,Zhang B,Yang X,Ge Q

    更新日期:2018-03-19 00:00:00

  • Identification of bacteriophages for biocontrol of the kiwifruit canker phytopathogen Pseudomonas syringae pv. actinidiae.

    abstract::Pseudomonas syringae pv. actinidiae is a reemerging pathogen which causes bacterial canker of kiwifruit (Actinidia sp.). Since 2008, a global outbreak of P. syringae pv. actinidiae has occurred, and in 2010 this pathogen was detected in New Zealand. The economic impact and the development of resistance in P. syringae ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.00062-14

    authors: Frampton RA,Taylor C,Holguín Moreno AV,Visnovsky SB,Petty NK,Pitman AR,Fineran PC

    更新日期:2014-04-01 00:00:00

  • Dissimilatory arsenate and sulfate reduction in sediments of two hypersaline, arsenic-rich soda lakes: Mono and Searles Lakes, California.

    abstract::A radioisotope method was devised to study bacterial respiratory reduction of arsenate in sediments. The following two arsenic-rich soda lakes in California were chosen for comparison on the basis of their different salinities: Mono Lake (approximately 90 g/liter) and Searles Lake (approximately 340 g/liter). Profiles...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.01066-06

    authors: Kulp TR,Hoeft SE,Miller LG,Saltikov C,Murphy JN,Han S,Lanoil B,Oremland RS

    更新日期:2006-10-01 00:00:00

  • Imidacloprid Decreases Honey Bee Survival Rates but Does Not Affect the Gut Microbiome.

    abstract::Accumulating evidence suggests that pesticides have played a role in the increased rate of honey bee colony loss. One of the most commonly used pesticides in the United States is the neonicotinoid imidacloprid. Although the primary mode of action of imidacloprid is on the insect nervous system, it has also been shown ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.00545-18

    authors: Raymann K,Motta EVS,Girard C,Riddington IM,Dinser JA,Moran NA

    更新日期:2018-06-18 00:00:00

  • Characterization of novel polycyclic aromatic hydrocarbon dioxygenases from the bacterial metagenomic DNA of a contaminated soil.

    abstract::Ring-hydroxylating dioxygenases (RHDs) play a crucial role in the biodegradation of a range of aromatic hydrocarbons found on polluted sites, including polycyclic aromatic hydrocarbons (PAHs). Current knowledge on RHDs comes essentially from studies on culturable bacterial strains, while compelling evidence indicates ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.01883-14

    authors: Chemerys A,Pelletier E,Cruaud C,Martin F,Violet F,Jouanneau Y

    更新日期:2014-11-01 00:00:00

  • Effects of a Bacterial Hay Preservative (Pediococcus pentosaceus) on Hay under Experimental Storage Conditions.

    abstract::The abundant growth of molds and thermophilic actinomycetes in stored hay decreases its quality and can be hazardous for the producer who inhales these contaminants when the moldy hay is fed in closed barns. These microbes are responsible for a respiratory disease called farmer's lung. Products, including bacterial cu...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.61.12.4240-4243.1995

    authors: Duchaine C,Lavoie MC,Cormier Y

    更新日期:1995-12-01 00:00:00

  • Development of multiple-locus variable-number tandem-repeat analysis for the molecular subtyping of Enterobacter sakazakii.

    abstract::The genomic content of Enterobacter sakazakii strain ATCC BAA-894 was analyzed for variable-number tandem repeats (VNTRs). In this study we report the development of a multiple-locus VNTR analysis (MLVA) strategy for the subtyping of E. sakazakii. The method is based on a GeneScan analysis of four VNTR loci labeled wi...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.01726-07

    authors: Mullane NR,Ryan M,Iversen C,Murphy M,O'Gaora P,Quinn T,Whyte P,Wall PG,Fanning S

    更新日期:2008-02-01 00:00:00