Differential Expression of Virulence Genes and Motility in Ralstonia (Pseudomonas) solanacearum during Exponential Growth.

Abstract:

:A complex network regulates virulence in Ralstonia solanacearum (formerly Pseudomonas solanacearum); central to this system is PhcA, a LysR-type transcriptional regulator. We report here that two PhcA-regulated virulence factors, endoglucanase (Egl) and acidic exopolysaccharide I (EPS I), and motility are expressed differentially during exponential growth in batch cultures. Tests with strains carrying lacZ fusions in a wild-type genetic background revealed that expression (on a per-cell basis) of phcA was constant but expression of egl and epsB increased 20- to 50-fold during multiplication from 1 x 10(sup7) to 5 x 10(sup8) CFU/ml. Expression of xpsR, an intermediate regulator downstream of PhcA in the regulatory cascade for eps expression, was similar to that of epsB and egl. Motility track photography revealed that all strains were essentially nonmotile at 10(sup6) CFU/ml. As cell density increased, 30 to 50% of wild-type cells were motile between 10(sup7) and 10(sup8) CFU/ml, but this population was again nonmotile at 10(sup9) CFU/ml. In contrast, about 60% of the cells of phcB and phcA mutants remained motile at 10(sup9) CFU/ml. Expression of phcB, which is not positively regulated by PhcA, was the inverse of epsB, egl, and xpsR (i.e., it decreased 20-fold at high cell density). PhcB is essential for production of an extracellular factor, tentatively identified as 3-hydroxypalmitic acid methyl ester (3-OH PAME), that might act as an exponential-phase signal to activate motility or expression of virulence genes. However, growth of the lacZ fusion strains in medium containing excess 3-OH PAME did not result in motility or expression of virulence genes at dramatically lower cell densities, suggesting that 3-OH PAME is not the only factor controlling these traits.

journal_name

Appl Environ Microbiol

authors

Clough SJ,Flavier AB,Schell MA,Denny TP

doi

10.1128/AEM.63.3.844-850.1997

keywords:

subject

Has Abstract

pub_date

1997-03-01 00:00:00

pages

844-50

issue

3

eissn

0099-2240

issn

1098-5336

journal_volume

63

pub_type

杂志文章
  • Application of recognition of individual genes-fluorescence in situ hybridization (RING-FISH) to detect nitrite reductase genes (nirK) of denitrifiers in pure cultures and environmental samples.

    abstract::Denitrification is an alternative type of anaerobic respiration in which nitrate is reduced to gaseous products via nitrite. The key step in this process is the reduction of nitrite to nitric oxide, which is catalyzed by two structurally different but functionally equivalent forms of nitrite reductase encoded by the n...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.01992-08

    authors: Pratscher J,Stichternoth C,Fichtl K,Schleifer KH,Braker G

    更新日期:2009-02-01 00:00:00

  • Nitrous oxide production and methane oxidation by different ammonia-oxidizing bacteria.

    abstract::Ammonia-oxidizing bacteria (AOB) are thought to contribute significantly to N2O production and methane oxidation in soils. Most of our knowledge derives from experiments with Nitrosomonas europaea, which appears to be of minor importance in most soils compared to Nitrosospira spp. We have conducted a comparative study...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.65.6.2679-2684.1999

    authors: Jiang QQ,Bakken LR

    更新日期:1999-06-01 00:00:00

  • Importance of laccase in vegetative growth of pleurotus Florida.

    abstract::Mycelial culture of Pleurotus florida produced highest extracellular laccase in optimum growth medium. At least two laccases (L(inf1) and L(inf2)) were shown to be present in the culture filtrate. Low-laccase-yielding mutants with impaired L(inf2) activity had poor mycelial growth and could not form fruit body, wherea...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.63.10.4120-4122.1997

    authors: Das N,Sengupta S,Mukherjee M

    更新日期:1997-10-01 00:00:00

  • Effects of surface area and flow rate on marine bacterial growth in activated carbon columns.

    abstract::The colonization of granular activated carbon columns by bacteria can have both beneficial and potentially detrimental consequences. Bacterial growth on the carbon surface can remove adsorbed organics and thus partially regenerate the carbon bed. However, growth can also increase the levels of bacteria in the column e...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.44.2.471-477.1982

    authors: Shimp RJ,Pfaender FK

    更新日期:1982-08-01 00:00:00

  • Abundance and distribution of Legionellaceae in Puerto Rican waters.

    abstract::Waters in marine and freshwater areas of Puerto Rico were analyzed for the presence of Legionella spp. by direct fluorescent antibody assay with guinea pig confirmation. Several species, including L. bozemanii, L. dumoffii, L. gormanii, L. longbeachae, L. micdadei, and L. pneumophila, were widely distributed among all...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.53.9.2231-2236.1987

    authors: Ortiz-Roque CM,Hazen TC

    更新日期:1987-09-01 00:00:00

  • Improved microbiological assay procedures for dihydrostreptomycin residues in milk and dairy products.

    abstract::Because dihydrostreptomycin can remain as a slowly removed antibiotic residue in dairy animals and because of the need for more sensitive procedures with which to provide information concerning antibiotic residues in food products, procedures were developed in more sensitive assays of dihydrostreptomycin in milk and s...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.35.3.517-520.1978

    authors: Inglis JM,Katz SE

    更新日期:1978-03-01 00:00:00

  • Novel Capsular Polysaccharide Loci and New Diagnostic Tools for High-Throughput Capsular Gene Typing in Streptococcus suis.

    abstract::Streptococcus suis is an important pathogen of pigs and may cause serious disease in humans. Serotyping is an important tool for detection and epidemiological studies of S. suis Thirty-three reference serotypes and nine novel cps loci (NCLs) are recognized in S. suis To gain a better understanding of the prevalence an...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.02102-16

    authors: Qiu X,Bai X,Lan R,Zheng H,Xu J

    更新日期:2016-11-21 00:00:00

  • Flexibility-Rigidity Coordination of the Dense Exopolysaccharide Matrix in Terrestrial Cyanobacteria Acclimated to Periodic Desiccation.

    abstract::A dense exopolysaccharide (EPS) matrix is crucial for cyanobacterial survival in terrestrial xeric environments, in which cyanobacteria undergo frequent expansion and shrinkage processes during environmental desiccation-rehydration cycles. However, it is unclear how terrestrial cyanobacteria coordinate the structural ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.01619-17

    authors: Liu W,Cui L,Xu H,Zhu Z,Gao X

    更新日期:2017-10-31 00:00:00

  • Cloning of the nprA gene for neutral protease A of Bacillus thuringiensis and effect of in vivo deletion of nprA on insecticidal crystal protein.

    abstract::The nprA gene, encoding Bacillus thuringiensis neutral protease A, was cloned by the use of gene-specific oligonucleotides. The size of neutral protease A deduced from the nprA sequence was 566 amino acids (60,982 Da). The cloned nprA gene was partially deleted in vitro, and the deleted allele, designated nprA3, was u...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.63.6.2311-2317.1997

    authors: Donovan WP,Tan Y,Slaney AC

    更新日期:1997-06-01 00:00:00

  • Exposure of Escherichia coli ATCC 12806 to sublethal concentrations of food-grade biocides influences its ability to form biofilm, resistance to antimicrobials, and ultrastructure.

    abstract::Escherichia coli ATCC 12806 was exposed to increasing subinhibitory concentrations of three biocides widely used in food industry facilities: trisodium phosphate (TSP), sodium nitrite (SNI), and sodium hypochlorite (SHY). The cultures exhibited an acquired tolerance to biocides (especially to SNI and SHY) after exposu...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.02283-13

    authors: Capita R,Riesco-Peláez F,Alonso-Hernando A,Alonso-Calleja C

    更新日期:2014-02-01 00:00:00

  • Degradation of pyrene, benz[a]anthracene, and benzo[a]pyrene by Mycobacterium sp. strain RJGII-135, isolated from a former coal gasification site.

    abstract::The degradation of three polycyclic aromatic hydrocarbons (PAH), pyrene (PYR), benz[a]anthracene (BAA), and benzo[a]pyrene (BaP), by Mycobacterium sp. strain RJGII-135 was studied. The bacterium was isolated from an abandoned coal gasification site soil by analog enrichment techniques and found to mineralize [14C]PYR....

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.62.1.13-19.1996

    authors: Schneider J,Grosser R,Jayasimhulu K,Xue W,Warshawsky D

    更新日期:1996-01-01 00:00:00

  • Function of native OmtA in vivo and expression and distribution of this protein in colonies of Aspergillus parasiticus.

    abstract::The activities of two enzymes, a 168-kDa protein and a 40-kDa protein, OmtA, purified from the filamentous fungus Aspergillus parasiticus were reported to convert the aflatoxin pathway intermediate sterigmatocystin to O-methylsterigmatocystin in vitro. Our initial goal was to determine if OmtA is necessary and suffici...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/aem.68.11.5718-5727.2002

    authors: Lee LW,Chiou CH,Linz JE

    更新日期:2002-11-01 00:00:00

  • Effects of Temperature and Relative Humidity on the Growth of and Enzyme Production by Actinomucor taiwanensis during Sufu Pehtze Preparation.

    abstract::The growth of and production of protease, alpha-amylase, alpha-galactosidase, and lipase by Actinomucor taiwanensis in relation to temperature and relative humidity during the preparation of sufu (Chinese cheese) pehtze were investigated. The incubation temperature, humidity, and cultivation time greatly affected the ...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.54.3.688-692.1988

    authors: Chou CC,Ho FM,Tsai CS

    更新日期:1988-03-01 00:00:00

  • Epidemiology of Cryptosporidium molnari in Spanish gilthead sea bream (Sparus aurata L.) and European sea bass (Dicentrarchus labrax L.) cultures: from hatchery to market size.

    abstract::A long-term epidemiological study of Cryptosporidium molnari in aquacultured European sea bass (ESB) and gilthead sea bream (GSB) was performed in different types of facilities on the Atlantic, Cantabric, and Mediterranean coasts. Four types of studies were carried out. In study A, fish raised from juveniles to market...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.71.1.131-139.2005

    authors: Sitjà-Bobadilla A,Padrós F,Aguilera C,Alvarez-Pellitero P

    更新日期:2005-01-01 00:00:00

  • Diversity, physiology, and niche differentiation of ammonia-oxidizing archaea.

    abstract::Nitrification, the aerobic oxidation of ammonia to nitrate via nitrite, has been suggested to have been a central part of the global biogeochemical nitrogen cycle since the oxygenation of Earth. The cultivation of several ammonia-oxidizing archaea (AOA) as well as the discovery that archaeal ammonia monooxygenase (amo...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章,评审

    doi:10.1128/AEM.01960-12

    authors: Hatzenpichler R

    更新日期:2012-11-01 00:00:00

  • Role of endoproteolytic dibasic proprotein processing in maturation of secretory proteins in Trichoderma reesei.

    abstract::Cell extracts of Trichoderma reesei exhibited dibasic endopeptidase activity toward the carboxylic side of KR, RR, and PR sequences. This activity was stimulated by the presence of Ca2+ ions and localized in vesicles of low bouyant density; it therefore exhibited some similarity to yeast Kex2. Analytical chromatofocus...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.64.9.3202-3208.1998

    authors: Goller SP,Schoisswohl D,Baron M,Parriche M,Kubicek CP

    更新日期:1998-09-01 00:00:00

  • Uptake and clearance of Vibrio vulnificus from Gulf coast oysters (Crassostrea virginica).

    abstract::Oysters collected in late winter, when they were free of Vibrio vulnificus, were exposed in the organism in the laboratory. The oysters effectively concentrated the bacteria from seawater, but when the inoculum was removed, the bacteria were rapidly cleared from the oyster tissues. These results suggest that V. vulnif...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.50.6.1548-1549.1985

    authors: Kelly MT,Dinuzzo A

    更新日期:1985-12-01 00:00:00

  • Effect of hydrogenase and mixed sulfate-reducing bacterial populations on the corrosion of steel.

    abstract::The importance of hydrogenase activity to corrosion of steel was assessed by using mixed populations of sulfate-reducing bacteria isolated from corroded and noncorroded oil pipelines. Biofilms which developed on the steel studs contained detectable numbers of sulfate-reducing bacteria (10 increasing to 10/0.5 cm). How...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.57.10.2804-2809.1991

    authors: Bryant RD,Jansen W,Boivin J,Laishley EJ,Costerton JW

    更新日期:1991-10-01 00:00:00

  • Anti-Candida albicans activity of Pichia anomala as determined by a growth rate reduction assay.

    abstract::Killer toxin activity of Pichia anomala WC65 appeared fungicidal for P. bimundalis WC38 and fungistatic for Candida albicans RC1. Inhibitory activity against sensitive C. albicans showed a linear relationship between toxin concentrations and the inverse of the reduced growth rates. The plot of toxin concentrations aga...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.54.5.1099-1103.1988

    authors: Sawant AD,Abdelal AT,Ahearn DG

    更新日期:1988-05-01 00:00:00

  • Appraisal of media and methods for assay of bacteriophages of lactic streptococci.

    abstract::To assess the relative merits of tryptone yeast extract agar, the same medium unbuffered, and medium M17 for the assay of nine bacteriophages of lactic streptococci, comparative plaque counts were made with an overlay of 3 or 9 ml. Four of the phages exhibited no significant difference in plating efficiency between me...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.40.4.798-802.1980

    authors: Keogh BP

    更新日期:1980-10-01 00:00:00

  • Genetic Diversity of Flavobacterium psychrophilum Isolates from Three Oncorhynchus spp. in the United States, as Revealed by Multilocus Sequence Typing.

    abstract:UNLABELLED:The use of a multilocus sequence typing (MLST) technique has identified the intraspecific genetic diversity of U.S. Flavobacterium psychrophilum, an important pathogen of salmonids worldwide. Prior to this analysis, little U.S. F. psychrophilum genetic information was known; this is of importance when consid...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.00411-16

    authors: Van Vliet D,Wiens GD,Loch TP,Nicolas P,Faisal M

    更新日期:2016-05-16 00:00:00

  • Evaluation of a strategy for Toxoplasma gondii oocyst detection in water.

    abstract::Several recent outbreaks of toxoplasmosis were related to drinking water. We propose a strategy for Toxoplasma oocyst detection as part of an approach to detecting multiple waterborne parasites, including Giardia and Cryptosporidium spp., by the U.S. Environmental Protection Agency method with the same sample. Water s...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.70.7.4035-4039.2004

    authors: Villena I,Aubert D,Gomis P,Ferté H,Inglard JC,Denis-Bisiaux H,Dondon JM,Pisano E,Ortis N,Pinon JM

    更新日期:2004-07-01 00:00:00

  • Different effects of transgenic maize and nontransgenic maize on nitrogen-transforming archaea and bacteria in tropical soils.

    abstract::The composition of the rhizosphere microbiome is a result of interactions between plant roots, soil, and environmental conditions. The impact of genetic variation in plant species on the composition of the root-associated microbiota remains poorly understood. This study assessed the abundances and structures of nitrog...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.01778-14

    authors: Cotta SR,Dias AC,Marriel IE,Andreote FD,Seldin L,van Elsas JD

    更新日期:2014-10-01 00:00:00

  • Decrease in culturability of Vibrio cholerae caused by glucose.

    abstract::The culturability of Vibrio cholerae O1 serotype Inaba strain 569B was decreased by the addition of glucose to cell suspensions in starvation media. A similar effect was observed with sucrose, maltose, and fructose. We term this inhibitory effect glucose shock. It was not observed with arabinose or xylose or with carb...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.61.7.2583-2588.1995

    authors: Shiba T,Hill RT,Straube WL,Colwell RR

    更新日期:1995-07-01 00:00:00

  • Purification and Characterization of a Substrate-Size-Recognizing Metalloendopeptidase from Streptococcus cremoris H61.

    abstract::During the ripening of Gouda-type cheese, two kinds of endopeptidases were found to participate in the degradation of alphas1-CN(f1-23), a specific product from alphas1-casein hydrolyzed by chymosin. One of the endopeptidases, lactic acid bacteria endopeptidase (LEP-II), which can recognize the size of its substrates,...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.53.10.2296-2302.1987

    authors: Yan TR,Azuma N,Kaminogawa S,Yamauchi K

    更新日期:1987-10-01 00:00:00

  • Linked redox precipitation of sulfur and selenium under anaerobic conditions by sulfate-reducing bacterial biofilms.

    abstract::A biofilm-forming strain of sulfate-reducing bacteria (SRB), isolated from a naturally occurring mixed biofilm and identified by 16S rDNA analysis as a strain of Desulfomicrobium norvegicum, rapidly removed 200 micro M selenite from solution during growth on lactate and sulfate. Elemental selenium and elemental sulfur...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/aem.69.12.7063-7072.2003

    authors: Hockin SL,Gadd GM

    更新日期:2003-12-01 00:00:00

  • Pattern of organotin inhibition of methanogenic bacteria.

    abstract::Seven organotin compounds and tin chloride were tested for their effects on the methanogenic bacteria Methanococcus thermolithotrophicus, Methanococcus deltae delta LH, and Methanosarcina barkeri 227. The methanogens were strongly inhibited by triethyltin, tripropyltin, and monophenyltin compounds, generally at concen...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.57.4.1189-1193.1991

    authors: Boopathy R,Daniels L

    更新日期:1991-04-01 00:00:00

  • Multiple syringe inoculator for agar plates.

    abstract::The design and operation of a manually operated multiple syringe inoculator was described. Either 9 or 21 inoculations of constant volume could be made simultaneously. Up to 100 plates could be inoculated in 15 min with excellent reproducibility. No contact occurred between the inoculating needles and the agar surface...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.33.4.982-985.1977

    authors: Kaneko T,Holder-Franklin M,Franklin M

    更新日期:1977-04-01 00:00:00

  • Unique substrate spectrum and PCR application of Nanoarchaeum equitans family B DNA polymerase.

    abstract::The known archaeal family B DNA polymerases are unable to participate in the PCR in the presence of uracil. Here, we report on a novel archaeal family B DNA polymerase from Nanoarchaeum equitans that can successfully utilize deaminated bases such as uracil and hypoxanthine and on its application to PCR. N. equitans fa...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.00624-08

    authors: Choi JJ,Song JG,Nam KH,Lee JI,Bae H,Kim GA,Sun Y,Kwon ST

    更新日期:2008-11-01 00:00:00

  • Genome-driven investigation of compatible solute biosynthesis pathways of Pseudomonas syringae pv. syringae and their contribution to water stress tolerance.

    abstract::The foliar pathogen Pseudomonas syringae pv. syringae exhibits an exceptional ability to survive on asymptomatic plants as an epiphyte. Intermittent wetting events on plants lead to osmotic and matric stresses which must be tolerated for survival as an epiphyte. In this study, we have applied bioinformatic, genetic, a...

    journal_title:Applied and environmental microbiology

    pub_type: 杂志文章

    doi:10.1128/AEM.00686-10

    authors: Kurz M,Burch AY,Seip B,Lindow SE,Gross H

    更新日期:2010-08-01 00:00:00