Improved microbiological assay procedures for dihydrostreptomycin residues in milk and dairy products.

abstract：:Eleven of 13 Enterobacteriaceae species tested grew in moist stored wheat, highlighting a potential risk of this energy-saving airtight storage method. When Hansenula anomala was coinoculated, all Enterobacteriaceae species were significantly inhibited after 2 months of storage, six of them to below the detection limi...

journal_title：Applied and environmental microbiology

authors： Olstorpe M,Schnürer J,Passoth V

更新日期：2012-01-01 00:00:00

abstract：:A gene encoding a putative 150-amino-acid methylglyoxal synthase was identified in Clostridium acetobutylicum ATCC 824. The enzyme was overexpressed in Escherichia coli and purified. Methylglyoxal synthase has a native molecular mass of 60 kDa and an optimum pH of 7.5. The Km and Vmax values for the substrate dihydrox...

journal_title：Applied and environmental microbiology

authors： Huang K,Rudolph FB,Bennett GN

更新日期：1999-07-01 00:00:00

abstract：:The occurrence of 10 pathogens and three fecal indicators was assessed by quantitative PCR in manures of Australian feedlot cattle. Most samples tested positive for one or more pathogens. For the dominant pathogens Campylobacter jejuni, Listeria monocytogenes, Giardia spp., Cryptosporidium spp., and eaeA-positive Esch...

journal_title：Applied and environmental microbiology

authors： Klein M,Brown L,Tucker RW,Ashbolt NJ,Stuetz RM,Roser DJ

更新日期：2010-10-01 00:00:00

abstract：:So far, only scarce and rather diffuse information is available on bacteriophages infecting members of the genus Bifidobacterium. In the current study, we investigated the genetic organization, phylogenetic relationships, and, in some cases, transcription profiles and inducibility of 19 prophage-like elements present ...

journal_title：Applied and environmental microbiology

authors： Ventura M,Turroni F,Lima-Mendez G,Foroni E,Zomer A,Duranti S,Giubellini V,Bottacini F,Horvath P,Barrangou R,Sela DA,Mills DA,van Sinderen D

更新日期：2009-11-01 00:00:00

abstract：:Hypoxia is encountered frequently by Candida albicans during systemic infection of the human host. We tested if hypoxia allows biofilm formation by C. albicans, which is a major cause of perseverance and antifungal resistance in C. albicans infections. Using an in vitro biofilm system, we unexpectedly discovered that ...

journal_title：Applied and environmental microbiology

authors： Stichternoth C,Ernst JF

更新日期：2009-06-01 00:00:00

abstract：:Bioaugmentation can alter the potential activity as well as the composition of the naturally occurring microbial biota during bioremediation of a contaminated site. The focus of the current study is the pollutant 17β-estradiol (E2), which can cause endocrine effects and is potentially harmful to aquatic biota and to p...

journal_title：Applied and environmental microbiology

authors： Iasur-Kruh L,Hadar Y,Minz D

更新日期：2011-06-01 00:00:00

abstract：:Light microscopy and a radioassay detected no significant difference in adherence of Kanagawa-positive and Kanagawa-negative strains of Vibrio parahaemolyticus to human epithelial cell lines. ...

journal_title：Applied and environmental microbiology

authors： Gingras SP,Howard LV

更新日期：1980-02-01 00:00:00

abstract：:Previously isolated dissimilatory perchlorate-reducing bacteria (DPRB) have been primarily affiliated with the Betaproteobacteria. Enrichments from the cathodic chamber of a bioelectrical reactor (BER) inoculated from creek water in Berkeley, CA, yielded a novel organism most closely related to a previously described ...

journal_title：Applied and environmental microbiology

authors： Thrash JC,Ahmadi S,Torok T,Coates JD

更新日期：2010-07-01 00:00:00

abstract：:Portions of published procedures for measurement of ergosterol content of decomposing plants were examined for their influence upon ergosterol yield. Common methods of treatment of plant samples prior to sterol extraction (e.g., oven drying, freezing, lyophilization) led to reduced recoveries of ergosterol (ca. 20 to ...

journal_title：Applied and environmental microbiology

authors： Newell SY,Arsuffi TL,Fallon RD

更新日期：1988-07-01 00:00:00

abstract：:A modification of the Adler capillary assay was used to evaluate the chemotactic responses of several denitrifiers to nitrate and nitrite. Strong positive chemotaxis was observed to NO(3) and NO(2) by soil isolates of Pseudomonas aeruginosa, Pseudomonas fluorescens, and Pseudomonas stutzeri, with the peak response occ...

journal_title：Applied and environmental microbiology

authors： Kennedy MJ,Lawless JG

更新日期：1985-01-01 00:00:00

abstract：:There is currently little information on nonphosphorylated sugar epimerases, which are of potential interest for producing rare sugars. We found a gene (the TM0416 gene) encoding a putative d-tagatose-3-epimerase-related protein from the hyperthermophilic bacterium Thermotoga maritima We overexpressed the TM0416 gene ...

journal_title：Applied and environmental microbiology

authors： Shin SM,Cao TP,Choi JM,Kim SB,Lee SJ,Lee SH,Lee DW

更新日期：2017-05-01 00:00:00

abstract：:Francisella tularensis is found in a wide variety of hosts and extrahost environments, making culture recovery a diagnostic challenge. Here we demonstrate improved recovery times and good sensitivity (90%) when cultures were inoculated on the site of an investigation using fresh tissues. For contaminated specimens, an...

journal_title：Applied and environmental microbiology

authors： Petersen JM,Schriefer ME,Gage KL,Montenieri JA,Carter LG,Stanley M,Chu MC

更新日期：2004-06-01 00:00:00

abstract：:The chromate reductase purified from Pseudomonas ambigua was found to be homologous with several nitroreductases. Escherichia coli DH5alpha and Vibrio harveyi KCTC 2720 nitroreductases were chosen for the present study, and their chromate-reducing activities were determined. A fusion between glutathione S-transferase ...

journal_title：Applied and environmental microbiology

authors： Kwak YH,Lee DS,Kim HB

更新日期：2003-08-01 00:00:00

abstract：:Two types of mesophilic, methanogenic bacteria were isolated in pure culture from anaerobic freshwater and marine mud with 2-propanol as the hydrogen donor. The freshwater strain (SK) was a Methanospirillum species, the marine, salt-requiring strain (CV), which had irregular coccoid cells, resembled Methanogenium sp. ...

journal_title：Applied and environmental microbiology

authors： Widdel F

更新日期：1986-05-01 00:00:00

abstract：:3-Deoxy-D-arabino-heptulosonate 7-phosphate (DAHP) synthase (EC 2.5.1.54) catalyzes the first step of the shikimate pathway that finally leads to the biosynthesis of aromatic amino acids phenylalanine (Phe), tryptophan (Trp), and tyrosine (Tyr). In Corynebacterium glutamicum ATCC 13032, two chromosomal genes, NCgl0950...

journal_title：Applied and environmental microbiology

authors： Liu YJ,Li PP,Zhao KX,Wang BJ,Jiang CY,Drake HL,Liu SJ

更新日期：2008-09-01 00:00:00

abstract：:Cellulose is an abundant and renewable biopolymer that can be used for biofuel generation; however, structural entrapment with other cell wall components hinders enzyme-substrate interactions, a key bottleneck for ethanol production. Biomass is routinely subjected to treatments that facilitate cellulase-cellulose cont...

journal_title：Applied and environmental microbiology

authors： Wang H,Squina F,Segato F,Mort A,Lee D,Pappan K,Prade R

更新日期：2011-08-01 00:00:00

abstract：:Lactococcin 972 (Lcn972) is a nonlantibiotic bacteriocin that inhibits septum biosynthesis in Lactococcus lactis rather than forming pores in the cytoplasmic membrane. In this study, a deeper analysis of the molecular basis of the mode of action of Lcn972 was performed. Of several lipid cell wall precursors, only lipi...

journal_title：Applied and environmental microbiology

authors： Martínez B,Böttiger T,Schneider T,Rodríguez A,Sahl HG,Wiedemann I

更新日期：2008-08-01 00:00:00

abstract：:Current models suggest that (i) filamentous bacteria are protected against predation by nanoflagellates, (ii) prey size is positively correlated with prey-predator contact probability, and (iii) contact probability is mainly responsible for size-selective predation by interception-feeding flagellates. We used five str...

journal_title：Applied and environmental microbiology

authors： Wu QL,Boenigk J,Hahn MW

更新日期：2004-01-01 00:00:00

abstract：:Ophthalmic acid (OA; l-γ-glutamyl-l-2-aminobutyryl-glycine) is an analog of glutathione (GSH; l-γ-glutamyl-l-cysteinyl-glycine) in which the cysteine moiety is replaced by l-2-aminobutyrate. OA is a useful peptide for the pharmaceutical and/or food industries. Herein, we report a method for the production of OA using ...

journal_title：Applied and environmental microbiology

authors： Ito T,Tokoro M,Hori R,Hemmi H,Yoshimura T

更新日期：2018-03-19 00:00:00

abstract：:The blacklegged tick, Ixodes scapularis, is of significant public health importance as a vector of Borrelia burgdorferi, the agent of Lyme borreliosis. The timing of seasonal activity of each immature I. scapularis life stage relative to the next is critical for the maintenance of B. burgdorferi because larvae must fe...

journal_title：Applied and environmental microbiology

authors： Gatewood AG,Liebman KA,Vourc'h G,Bunikis J,Hamer SA,Cortinas R,Melton F,Cislo P,Kitron U,Tsao J,Barbour AG,Fish D,Diuk-Wasser MA

更新日期：2009-04-01 00:00:00

abstract：:The impact of feed supplementation with bambermycin, monensin, narasin, virginiamycin, chlortetracycline, penicillin, salinomycin, and bacitracin on the distribution of Escherichia coli pathotypes in broiler chickens was investigated using an E. coli virulence DNA microarray. Among 256 E. coli isolates examined, 59 (2...

journal_title：Applied and environmental microbiology

authors： Bonnet C,Diarrassouba F,Brousseau R,Masson L,Topp E,Diarra MS

更新日期：2009-11-01 00:00:00

abstract：:In this study, the genes involved in the initial attack on fluorene by Sphingomonas sp. strain LB126 were investigated. The alpha and beta subunits of a dioxygenase complex (FlnA1-FlnA2), showing 63 and 51% sequence identity, respectively, to the subunits of an angular dioxygenase from the gram-positive dibenzofuran d...

journal_title：Applied and environmental microbiology

authors： Schuler L,Ní Chadhain SM,Jouanneau Y,Meyer C,Zylstra GJ,Hols P,Agathos SN

更新日期：2008-02-01 00:00:00

abstract：:Identification and classification of Vibrio species have relied upon band pattern methods (e.g., amplified fragment length polymorphism) and DNA-DNA hybridization. However, data generated by these methods cannot be used to build an online electronic taxonomy. In order to overcome these limitations, we developed the fi...

journal_title：Applied and environmental microbiology

authors： Thompson FL,Gomez-Gil B,Vasconcelos AT,Sawabe T

更新日期：2007-07-01 00:00:00

abstract：:Since the food-borne pathogen Listeria monocytogenes is common in dairy farm environments, it is likely that phages infecting this bacterium ("listeriaphages") are abundant on dairy farms. To better understand the ecology and diversity of listeriaphages on dairy farms and to develop a diverse phage collection for furt...

journal_title：Applied and environmental microbiology

authors： Vongkamjan K,Switt AM,den Bakker HC,Fortes ED,Wiedmann M

更新日期：2012-12-01 00:00:00

abstract：:Methods for the production and regeneration of Lactobacillus casei protoplasts are described. Protoplasts of L. casei strains were obtained by treatment with mutanolysin or with mutanolysin and lysozyme together in a protoplast formation buffer containing 0.02 M HEPES (N-2-hydroxyethylpiperazine-N'-2-ethanesulfonic ac...

journal_title：Applied and environmental microbiology

authors： Lee-Wickner LJ,Chassy BM

更新日期：1984-11-01 00:00:00

abstract：:To overcome the limited availability of antibiotic resistance markers in filamentous fungi, we adapted the FLP/FRT recombination system from the yeast Saccharomyces cerevisiae for marker recycling. We tested this system in the penicillin producer Penicillium chrysogenum using different experimental approaches. In a tw...

journal_title：Applied and environmental microbiology

authors： Kopke K,Hoff B,Kück U

更新日期：2010-07-01 00:00:00

abstract：:Presently, the only effective treatment for celiac disease is a life-long gluten-free diet. In this work, we used a new mixture of selected sourdough lactobacilli and fungal proteases to eliminate the toxicity of wheat flour during long-time fermentation. Immunological (R5 antibody-based sandwich and competitive enzym...

journal_title：Applied and environmental microbiology

authors： Rizzello CG,De Angelis M,Di Cagno R,Camarca A,Silano M,Losito I,De Vincenzi M,De Bari MD,Palmisano F,Maurano F,Gianfrani C,Gobbetti M

更新日期：2007-07-01 00:00:00

abstract：:Flow cytometry, using fluorescein-bound specific antibodies and propidium iodide, was shown to be effective in detecting Legionella spp. in cooling tower waters. The procedure was quicker and less labor intensive than fluorescent microscopy. The use of these procedures also identified qualitative differences, perhaps ...

journal_title：Applied and environmental microbiology

authors： Tyndall RL,Hand RE Jr,Mann RC,Evans C,Jernigan R

更新日期：1985-04-01 00:00:00

abstract：:Two constructs derived from the alpha-amylase gene (amyA) of Lactobacillus amylovorus were expressed in Lactobacillus plantarum, and their expression products were purified, characterized, and compared. These products correspond to the complete (AmyA) and truncated (AmyADelta) forms of alpha-amylase; AmyADelta lacks t...

journal_title：Applied and environmental microbiology

authors： Rodriguez Sanoja R,Morlon-Guyot J,Jore J,Pintado J,Juge N,Guyot JP

更新日期：2000-08-01 00:00:00

abstract：:In order to identify antifungal compounds from natural sources to be used as ingredients in the bakery industry, water/salt-soluble extracts (WSE) from different legume flour hydrolysates obtained by the use of a fungal protease were assayed against Penicillium roqueforti DPPMAF1. The agar diffusion assays allowed the...

journal_title：Applied and environmental microbiology

authors： Rizzello CG,Lavecchia A,Gramaglia V,Gobbetti M

更新日期：2015-06-15 00:00:00