Characterization of methylglyoxal synthase from Clostridium acetobutylicum ATCC 824 and its use in the formation of 1, 2-propanediol.

Abstract:

:A gene encoding a putative 150-amino-acid methylglyoxal synthase was identified in Clostridium acetobutylicum ATCC 824. The enzyme was overexpressed in Escherichia coli and purified. Methylglyoxal synthase has a native molecular mass of 60 kDa and an optimum pH of 7.5. The Km and Vmax values for the substrate dihydroxyacetone phosphate were 0.53 mM and 1.56 mmol min(-1) microgram(-1), respectively. When E. coli glycerol dehydrogenase was coexpressed with methylglyoxal synthase in E. coli BL21(DE3), 3.9 mM 1,2-propanediol was produced.

journal_name

Appl Environ Microbiol

authors

Huang K,Rudolph FB,Bennett GN

doi

10.1128/AEM.65.7.3244-3247.1999

keywords:

subject

Has Abstract

pub_date

1999-07-01 00:00:00

pages

3244-7

issue

7

eissn

0099-2240

issn

1098-5336

journal_volume

65

pub_type

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