Direct effects of UV-B radiation on the freshwater heterotrophic nanoflagellate Paraphysomonas sp.

abstract：:Vibrio vulnificus biotype 2 is a primary pathogen for eels and, as has recently been suggested, an opportunistic pathogen for humans. In this study we have investigated the ability of V. vulnificus biotype 2 to obtain iron by siderophore-mediated mechanisms and evaluated the importance of free iron in vibriosis. The v...

journal_title：Applied and environmental microbiology

authors： Biosca EG,Fouz B,Alcaide E,Amaro C

更新日期：1996-03-01 00:00:00

abstract：:Severe intoxications caused by the Bacillus cereus emetic toxin cereulide can hardly be prevented due to the ubiquitous distribution and heat resistance of spores and the extreme thermal and chemical stability of cereulide. It would therefore be desirable to inhibit cereulide synthesis during food manufacturing proces...

journal_title：Applied and environmental microbiology

authors： Frenzel E,Letzel T,Scherer S,Ehling-Schulz M

更新日期：2011-02-01 00:00:00

abstract：:We applied HNPP (2-hydroxy-3-naphthoic acid-2'-phenylanilide phosphate) to direct in situ PCR for the routine detection of specific bacterial cells at the single-cell level. PCR was performed on glass slides with digoxigenin-labeled dUTP. The digoxigenin-labeled PCR products were detected with alkaline phosphatase-lab...

journal_title：Applied and environmental microbiology

authors： Tani K,Kurokawa K,Nasu M

更新日期：1998-04-01 00:00:00

abstract：:In a medium containing ammonia, proteose peptone, and cysteine as nitrogen sources, 17 of 24 Bacteroidaceae strains, 3 of Selenomonas strains, 1 of 7 curved rods, 3 of 7 Spirochaetaceae strains, 8 of 20 Eubacterium strains, 8 of 13 Peptococcaceae strains, 3 of 4 Clostridium strains, 19 of 20 Enterobacteriaceae strains...

journal_title：Applied and environmental microbiology

authors： Takahashi M,Benno Y,Mitsuoka T

更新日期：1980-01-01 00:00:00

abstract：:The influence of trichloroethene (TCE; 0 to 65 mg/liter) and 1,1,1-trichloroethane (1,1,1-TCA; 0 to 103 mg/liter) on methane consumption of a mixed culture of methane-oxidizing bacteria was studied in laboratory batch experiments. Increasing concentrations of TCE or 1,1,1-TCA resulted in decreasing methane consumption...

journal_title：Applied and environmental microbiology

authors： Broholm K,Jensen BK,Christensen TH,Olsen L

更新日期：1990-08-01 00:00:00

abstract：:Five strains of mesophilic, facultatively organotrophic, ore-leaching eubacteria were isolated from solfatara fields in Iceland and a uranium mine in the Federal Republic of Germany. The new organisms are aerobic gram-negative rods. They can use sulfidic ores or elemental sulfur as sole energy source, indicating that ...

journal_title：Applied and environmental microbiology

authors： Huber H,Stetter KO

更新日期：1990-02-01 00:00:00

abstract：:The Lactobacillus johnsonii VPI 11088 groESL operon was localized on the chromosome near the insertion element IS1223. The operon was initially cloned as a series of three overlapping PCR fragments, which were sequenced and used to design primers to amplify the entire operon. The amplified fragment was used as a probe...

journal_title：Applied and environmental microbiology

authors： Walker DC,Girgis HS,Klaenhammer TR

更新日期：1999-07-01 00:00:00

abstract：:Since 2006, Geomyces destructans, the causative agent of white nose syndrome (WNS), has killed over 5.7 million bats in North America. The current hypothesis suggests that this novel fungus is an invasive species from Europe, but little is known about the diversity within the genus Geomyces and its distribution on bat...

journal_title：Applied and environmental microbiology

authors： Johnson LJ,Miller AN,McCleery RA,McClanahan R,Kath JA,Lueschow S,Porras-Alfaro A

更新日期：2013-09-01 00:00:00

abstract：:The thermal inactivation kinetics of Salmonella enteritidis PT4 between 49 and 60 degrees C were investigated. Using procedures designed to eliminate methodological artifacts, we found that the death kinetics deviated from the accepted model of first-order inactivation. When we used high-density stationary-phase popul...

journal_title：Applied and environmental microbiology

authors： Humpheson L,Adams MR,Anderson WA,Cole MB

更新日期：1998-02-01 00:00:00

abstract：:An extracellular serine protease produced by a mutant, M1, derived from Streptomyces albogriseolus S-3253 that no longer produces a protease inhibitor (Streptomyces subtilisin inhibitor [SSI]) was isolated. A 20-kDa protein was purified by its affinity for SSI and designated SAM-P20. The amino acid sequence of the ami...

journal_title：Applied and environmental microbiology

authors： Taguchi S,Odaka A,Watanabe Y,Momose H

更新日期：1995-01-01 00:00:00

abstract：:Stability of the plasmid pKK223-200 in Escherichia coli JM105 was studied for both free and immobilized cells during continuous culture. The relationship between plasmid copy number, xylanase activity, which was coded for by the plasmid, and growth rate and culture conditions involved complex interactions which determ...

journal_title：Applied and environmental microbiology

authors： de Taxis du Poët P,Arcand Y,Bernier R Jr,Barbotin JN,Thomas D

更新日期：1987-07-01 00:00:00

abstract：:Bacteria forming light-organ symbiosis with deep-sea chlorophthalmid fishes (Aulopiformes: Chlorophthalmidae) are considered to belong to the species Photobacterium phosphoreum. The identification of these bacteria as P. phosphoreum, however, was based exclusively on phenotypic traits, which may not discriminate betwe...

journal_title：Applied and environmental microbiology

authors： Dunlap PV,Ast JC

更新日期：2005-02-01 00:00:00

abstract：:The wood protection industry has refined their products from chrome-, copper-, and arsenate-based wood preservatives toward solely copper-based preservatives in combination with organic biocides. One of these is Cu-HDO, containing the chelation product of copper and N-cyclohexyldiazenium dioxide (HDO). In this study, ...

journal_title：Applied and environmental microbiology

authors： Jakobs-Schönwandt D,Mathies H,Abraham WR,Pritzkow W,Stephan I,Noll M

更新日期：2010-12-01 00:00:00

abstract：:In this study, the feasibility of fluorescence lifetime imaging (FLIM) for measurement of RNA:DNA ratios in microorganisms was assessed. The fluorescence lifetime of a nucleic acid-specific probe (SYTO 13) was used to directly measure the RNA:DNA ratio inside living bacterial cells. In vitro, SYTO 13 showed shorter fl...

journal_title：Applied and environmental microbiology

authors： Walczysko P,Kuhlicke U,Knappe S,Cordes C,Neu TR

更新日期：2008-01-01 00:00:00

abstract：:In order to explore the biochemical scope of ochratoxin A-producing penicillia, we screened 48 Penicillium verrucosum isolates for the production of secondary metabolites. Fungal metabolites were analyzed by high-pressure liquid or gas chromatography coupled to diode array detection or mass spectrometry. The following...

journal_title：Applied and environmental microbiology

authors： Larsen TO,Svendsen A,Smedsgaard J

更新日期：2001-08-01 00:00:00

abstract：:Carp interstitial nephritis and gill necrosis virus (CNGV) is an unclassified large DNA virus that morphologically resembles members of the Herpesviridae but contains a large (ca. approximately 280-kbp) linear double-stranded DNA. This virus has also been named koi herpesvirus, koi herpes-like virus, and cyprinid herp...

journal_title：Applied and environmental microbiology

authors： Dishon A,Perelberg A,Bishara-Shieban J,Ilouze M,Davidovich M,Werker S,Kotler M

更新日期：2005-11-01 00:00:00

abstract：:Listeria monocytogenes is a foodborne pathogen whose survival in food processing environments may be associated with its tolerance to desiccation. To probe the molecular mechanisms used by this bacterium to adapt to desiccation stress, a transposon library of 11,700 L. monocytogenes mutants was screened, using a micro...

journal_title：Applied and environmental microbiology

authors： Hingston PA,Piercey MJ,Truelstrup Hansen L

更新日期：2015-08-15 00:00:00

abstract：:In this study, food samples were intentionally contaminated with Escherichia coli O157:H7, and then DNA was isolated by using four commercial kits. The isolated DNA samples were compared by using real-time PCR detection of the Shiga toxin genes. The four kits tested worked similarly. ...

journal_title：Applied and environmental microbiology

authors： Heller LC,Davis CR,Peak KK,Wingfield D,Cannons AC,Amuso PT,Cattani J

更新日期：2003-03-01 00:00:00

abstract：:Wood extractives, commonly referred to as pitch, cause major problems in the manufacturing of pulp and paper. Treatment of nonsterile southern yellow pine chips for 14 days with Pseudomonas fluorescens, Pseudomonas sp., Xanthomonas campestris, and Serratia marcescens reduced wood extractives by as much as 40%. Control...

journal_title：Applied and environmental microbiology

authors： Burnes TA,Blanchette RA,Farrell RL

更新日期：2000-12-01 00:00:00

abstract：:In the presence of added sulfate, 2,4-dichlorophenol and 4-chlorophenol were transformed stoichiometrically to 4-chlorophenol and phenol, respectively, in anaerobic freshwater lake sediments between 18 and 40 degrees C. The concomitantly occurring sulfate reduction reduced the initial sulfate concentration from 25 mM ...

journal_title：Applied and environmental microbiology

authors： Kohring GW,Zhang XM,Wiegel J

更新日期：1989-10-01 00:00:00

abstract：:The degradation of three polycyclic aromatic hydrocarbons (PAH), pyrene (PYR), benz[a]anthracene (BAA), and benzo[a]pyrene (BaP), by Mycobacterium sp. strain RJGII-135 was studied. The bacterium was isolated from an abandoned coal gasification site soil by analog enrichment techniques and found to mineralize [14C]PYR....

journal_title：Applied and environmental microbiology

authors： Schneider J,Grosser R,Jayasimhulu K,Xue W,Warshawsky D

更新日期：1996-01-01 00:00:00

abstract：:Various physical and chemical parameters were monitored to evaluate their influence on the microbial communities present in composting municipal sewage sludge. Temperature, moisture content, depth, pH, protein content, total nitrogen, total carbon, lipid phosphate biomass, and the rates of microbial incorporation of s...

journal_title：Applied and environmental microbiology

authors： McKinley VL,Vestal JR

更新日期：1985-12-01 00:00:00

abstract：:Hepatitis A virus (HAV) and Norwalk-like virus (NLV) were detected by reverse transcription-PCR in clams imported into the United States from China. An epidemiological investigation showed that these clams were associated with five cases of Norwalk-like gastroenteritis in New York State in August 2000 (Food and Drug A...

journal_title：Applied and environmental microbiology

authors： Kingsley DH,Meade GK,Richards GP

更新日期：2002-08-01 00:00:00

abstract：:The lytic action of glycerol and sucrose esters of fatty acids with different carbon chain lengths on the exponentially growing cells of Bacillus subtilis 168 was investigated. Of each series of esters, glycerol dodecanoate and sucrose hexadecanoate were the most active. Lysis at 1 h after the addition of 0.1 mM glyce...

journal_title：Applied and environmental microbiology

authors： Tsuchido T,Ahn YH,Takano M

更新日期：1987-03-01 00:00:00

abstract：:Volume 61, no. 12, p. 4505, Introduction, column 1, line 13, and column 2, line 5: reference 19, which refers to a toxicity test based on the inhibition of (beta)-glucuronidase, not (beta)-galactosidase, in E. coli, should not be cited. [This corrects the article on p. 4505 in vol. 61.]. ...

journal_title：Applied and environmental microbiology

authors： Van Poucke SO,Nelis HJ

更新日期：1996-02-01 00:00:00

abstract：:Yeasts grown in the presence of benzoic acid tolerated 40 to 100% higher benzoic acid concentrations than did those grown in the absence of weak-acid-type preservatives. They also accumulated less benzoate in the presence of glucose. In chemostat cultures, benzoic acid reduced growth yield and the rate of cell product...

journal_title：Applied and environmental microbiology

authors： Warth AD

更新日期：1988-08-01 00:00:00

abstract：:Methanogens can use H(2) produced by cathodic depolarization-mediated oxidation of elemental iron to produce methane. Thermodynamic consideration of the cathodic depolarization mechanism predicts more oxidation of Fe at lower pH. Methanogenic responses to pH by Methanococcus deltae, Methanococcus thermolithotrophicus,...

journal_title：Applied and environmental microbiology

authors： Boopathy R,Daniels L

更新日期：1991-07-01 00:00:00

abstract：:Two Baeyer-Villiger monooxygenases (BVMOs), designated BoBVMO and AmBVMO, were discovered from Bradyrhizobium oligotrophicum and Aeromicrobium marinum, respectively. Both monooxygenases displayed novel features for catalyzing the asymmetric sulfoxidation of bulky and pharmaceutically relevant thioethers. Evolutionary ...

journal_title：Applied and environmental microbiology

authors： Zhang Y,Liu F,Xu N,Wu YQ,Zheng YC,Zhao Q,Lin G,Yu HL,Xu JH

更新日期：2018-07-02 00:00:00

abstract：:Herein, we report that a modified gentamicin cassette and a PCR-based method can be used for targeted mutagenesis of the oral spirochete Treponema denticola. This approach minimizes polar effects and spontaneous antibiotic resistance. Therefore, it can serve as a reliable tool for genetic manipulation of T. denticola....

journal_title：Applied and environmental microbiology

authors： Bian J,Fenno JC,Li C

更新日期：2012-03-01 00:00:00

abstract：:The purpose of this study was to examine host distribution patterns among fecal bacteria in the order Bacteroidales, with the goal of using endemic sequences as markers for fecal source identification in aquatic environments. We analyzed Bacteroidales 16S rRNA gene sequences from the feces of eight hosts: human, bovin...

journal_title：Applied and environmental microbiology

authors： Dick LK,Bernhard AE,Brodeur TJ,Santo Domingo JW,Simpson JM,Walters SP,Field KG

更新日期：2005-06-01 00:00:00