An improved enzyme-linked immunosorbent assay for whole-cell determination of methanogens in samples from anaerobic reactors.

abstract：:Botulinum neurotoxin (BoNT), produced by neurotoxigenic clostridia, is the most potent biological toxin known and the causative agent of the paralytic disease botulism. The nutritional, environmental, and genetic regulation of BoNT synthesis, activation, stability, and toxin complex (TC) formation is not well studied....

journal_title：Applied and environmental microbiology

authors： Fredrick CM,Lin G,Johnson EA

更新日期：2017-06-16 00:00:00

abstract：:We describe here a new enzyme-coupled assay for the quantitation of d-xylose using readily available enzymes that allows kinetic evaluation of hemicellulolytic enzymes using natural xylooligosaccharide substrates. Hydrogen peroxide is generated as an intermediary analyte, which allows flexibility in the choice of the ...

journal_title：Applied and environmental microbiology

authors： Wagschal K,Franqui-Espiet D,Lee CC,Robertson GH,Wong DW

更新日期：2005-09-01 00:00:00

abstract：:Isolation and cultivation are a crucial step in elucidating the physiology, biogeochemistry, and ecosystem role of microorganisms. Many abundant marine bacteria, including the widespread Roseobacter clade-affiliated (RCA) cluster group, have not been cultured with traditional methods. Using novel techniques of coculti...

journal_title：Applied and environmental microbiology

authors： Mayali X,Franks PJ,Azam F

更新日期：2008-05-01 00:00:00

abstract：:The composition of predominant soil bacteria during grassland succession was investigated in the Dutch Drentse A area. Five meadows, taken out of agricultural production at different time points, and one currently fertilized plot represented different stages of grassland succession. Since fertilization and agricultura...

journal_title：Applied and environmental microbiology

authors： Felske A,Wolterink A,Van Lis R,De Vos WM,Akkermans AD

更新日期：2000-09-01 00:00:00

abstract：:Although only partially understood, multicellular behavior is relatively common in bacterial pathogens. Bacterial aggregates can resist various host defenses and colonize their environment more efficiently than planktonic cells. For the waterborne pathogen Legionella pneumophila, little is known about the roles of aut...

journal_title：Applied and environmental microbiology

authors： Abdel-Nour M,Duncan C,Prashar A,Rao C,Ginevra C,Jarraud S,Low DE,Ensminger AW,Terebiznik MR,Guyard C

更新日期：2014-02-01 00:00:00

abstract：:Alcaligenes eutrophus was grown autotrophically in 23-liter batch cultures in a controlled H2-O2-CO2 atmosphere. It was demonstrated that the need for periodic supplements of individual nutrients could be anticipated before cell growth depleted these nutrients to the point of becoming growth rate limiting. As a result...

journal_title：Applied and environmental microbiology

authors： Repaske R,Mayer R

更新日期：1976-10-01 00:00:00

abstract：:Lactobacillus gasseri ATCC 33323, Lactobacillus salivarius subsp. salivarius UCC 118, and Lactobacillus casei ATCC 334 contain one (LgaI), four (Sal1, Sal2, Sal3, Sal4), and one (Lca1) distinguishable prophage sequences, respectively. Sequence analysis revealed that LgaI, Lca1, Sal1, and Sal2 prophages belong to the g...

journal_title：Applied and environmental microbiology

authors： Ventura M,Canchaya C,Bernini V,Altermann E,Barrangou R,McGrath S,Claesson MJ,Li Y,Leahy S,Walker CD,Zink R,Neviani E,Steele J,Broadbent J,Klaenhammer TR,Fitzgerald GF,O'toole PW,van Sinderen D

更新日期：2006-05-01 00:00:00

abstract：:The known archaeal family B DNA polymerases are unable to participate in the PCR in the presence of uracil. Here, we report on a novel archaeal family B DNA polymerase from Nanoarchaeum equitans that can successfully utilize deaminated bases such as uracil and hypoxanthine and on its application to PCR. N. equitans fa...

journal_title：Applied and environmental microbiology

authors： Choi JJ,Song JG,Nam KH,Lee JI,Bae H,Kim GA,Sun Y,Kwon ST

更新日期：2008-11-01 00:00:00

abstract：:The industrial production of beta-carotene with the zygomycete Blakeslea trispora involves the joint cultivation of mycelia of opposite sex in the presence of beta-ionone and other chemical activators. We have obtained improved strains by mutation and heterokaryosis. We chose wild strains on the basis of their growth ...

journal_title：Applied and environmental microbiology

authors： Mehta BJ,Obraztsova IN,Cerdá-Olmedo E

更新日期：2003-07-01 00:00:00

abstract：:Enterotoxin-positive (Ent+) and enterotoxin-negative (Ent-) strains of Clostridium perfringens were cultured in Duncan-Strong sporulation medium containing starch at 37 and 46 degrees C. At 37 degrees C, all strains degraded starch and sporulated well. However, only Ent- strains could hydrolyze starch, grow extensivel...

journal_title：Applied and environmental microbiology

authors： García-Alvarado JS,Rodriguez MA,Labbé RG

更新日期：1992-01-01 00:00:00

abstract：:Thirty years of intensive research have significantly contributed to our understanding of Helicobacter pylori biology and pathogenesis. However, the lack of convenient genetic tools, in particular the limited effectiveness of available reporter systems, has notably limited the toolbox for fundamental and applied studi...

journal_title：Applied and environmental microbiology

authors： Vannini A,Agriesti F,Mosca F,Roncarati D,Scarlato V,Danielli A

更新日期：2012-09-01 00:00:00

abstract：:Three 16S rRNA hybridization probes were developed and tested for genus-specific detection of Bifidobacterium species in the human fecal flora. Variable regions V2, V4, and V8 of the 16S rRNA contained sequences unique to this genus and proved applicable as target sites for oligodeoxynucleotide probes. Determination o...

journal_title：Applied and environmental microbiology

authors： Langendijk PS,Schut F,Jansen GJ,Raangs GC,Kamphuis GR,Wilkinson MH,Welling GW

更新日期：1995-08-01 00:00:00

abstract：:White rot fungi can oxidize high-molecular-weight polycyclic aromatic hydrocarbons (PAH) rapidly to polar metabolites, but only limited mineralization takes place. The objectives of this study were to determine if the polar metabolites can be readily mineralized by indigenous microflora from several inoculum sources, ...

journal_title：Applied and environmental microbiology

authors： Kotterman MJ,Vis EH,Field JA

更新日期：1998-08-01 00:00:00

abstract：:The development of resistance in foodborne pathogens to food preservation techniques is an issue of increasing concern, especially in minimally processed foods where safety relies on hurdle technology. In this context, mild heat can be used in combination with so-called nonthermal processes, such as high hydrostatic p...

journal_title：Applied and environmental microbiology

authors： Gayán E,Cambré A,Michiels CW,Aertsen A

更新日期：2016-10-27 00:00:00

abstract：:We report here the cloning and sequencing of the gene for proline dehydrogenase (putA) of Bradyrhizobium japonicum. An open reading frame coding for 1,016 amino acids was identified. The B. japonicum gene codes for a bifunctional protein with proline dehydrogenase and pyrroline-5-carboxylate (P5C) dehydrogenase activi...

journal_title：Applied and environmental microbiology

authors： Straub PF,Reynolds PH,Althomsons S,Mett V,Zhu Y,Shearer G,Kohl DH

更新日期：1996-01-01 00:00:00

abstract：:Three crude oil bioremediation techniques were applied in a randomized block field experiment simulating a coastal oil spill. Four treatments (no oil control, oil alone, oil plus nutrients, and oil plus nutrients plus an indigenous inoculum) were applied. In situ microbial community structures were monitored by phosph...

journal_title：Applied and environmental microbiology

authors： MacNaughton SJ,Stephen JR,Venosa AD,Davis GA,Chang YJ,White DC

更新日期：1999-08-01 00:00:00

abstract：:This investigation introduces the use of an immunoglobulin A mouse myeloma protein for the detection of Salmonella spp. in milk. The immunoglobulin A protein M467 reacts with flagellin from a wide variety of serotypes. Two assays were developed which used an enzyme-linked immunosorbent assay (ELISA) and M467. Alkaline...

journal_title：Applied and environmental microbiology

authors： Smith AM,Jones C

更新日期：1983-10-01 00:00:00

abstract：:A 2.2-kb fragment containing a replicon from pBtoxis, the large plasmid that encodes the insecticidal endotoxins of Bacillus thuringiensis subsp. israelensis, was identified, cloned, and sequenced. This fragment contains cis elements, including iterons, found in replication origins of other large plasmids and suggests...

journal_title：Applied and environmental microbiology

authors： Tang M,Bideshi DK,Park HW,Federici BA

更新日期：2006-11-01 00:00:00

abstract：:Two activities involved in terminal pathway conversion of sterigmatocystin to aflatoxin B1 were isolated from an aflatoxin-nonproducing mutant of Aspergillus parasiticus (avn-1), and the time course of appearance of the activities in culture was determined. Subcellular fractionation of fungal mycelia resolved the two ...

journal_title：Applied and environmental microbiology

authors： Cleveland TE,Lax AR,Lee LS,Bhatnagar D

更新日期：1987-07-01 00:00:00

abstract：:We previously demonstrated that xanthobaccin A from the rhizoplane bacterium Lysobacter sp. strain SB-K88 suppresses damping-off disease caused by Pythium sp. in sugar beet. In this study we focused on modes of Lysobacter sp. strain SB-K88 root colonization and antibiosis of the bacterium against Aphanomyces cochlioid...

journal_title：Applied and environmental microbiology

authors： Islam MT,Hashidoko Y,Deora A,Ito T,Tahara S

更新日期：2005-07-01 00:00:00

abstract：:A European baseline survey during the years 2005 and 2006 has revealed that the monophasic Salmonella enterica subsp. enterica serovar 4,12:d:- was, with a prevalence of 23.6%, the most frequently isolated serovar in German broiler flocks. In Denmark and the United Kingdom, its serovar prevalences were 15.15% and 2.8%...

journal_title：Applied and environmental microbiology

authors： Huehn S,Bunge C,Junker E,Helmuth R,Malorny B

更新日期：2009-02-01 00:00:00

abstract：:Thermal inactivation of desiccation-adapted Salmonella spp. in aged chicken litter was investigated in comparison with that in a nonadapted control to examine potential cross-tolerance of desiccation-adapted cells to heat treatment. A mixture of four Salmonella serovars was inoculated into the finished compost with 20...

journal_title：Applied and environmental microbiology

authors： Chen Z,Diao J,Dharmasena M,Ionita C,Jiang X,Rieck J

更新日期：2013-11-01 00:00:00

abstract：:Heat-treated Escherichia coli producing Thermus polyphosphate kinase regenerated ATP by using exogenous polyphosphate. This recombinant could be used as a platform to produce valuable compounds in combination with thermostable phosphorylating or energy-requiring enzymes. In this work, we demonstrated the production of...

journal_title：Applied and environmental microbiology

authors： Iwamoto S,Motomura K,Shinoda Y,Urata M,Kato J,Takiguchi N,Ohtake H,Hirota R,Kuroda A

更新日期：2007-09-01 00:00:00

abstract：:Wastewater disinfection is used in many countries for reducing fecal coliform levels in effluents. Disinfection is therefore frequently used to improve recreational bathing waters which do not comply with microbiological standards. It is unknown whether human enteric viruses (which are responsible for waterborne disea...

journal_title：Applied and environmental microbiology

authors： Tree JA,Adams MR,Lees DN

更新日期：2003-04-01 00:00:00

abstract：:Formation of the lateral flagella by Vibrio parahaemolyticus was inhibited under an alkaline condition of the medium. However, flagellation of the polar monotrichous flagellum was not affected in the same condition. Flagellation of the lateral flagella depended on the pH of the medium. ...

journal_title：Applied and environmental microbiology

authors： Kimura K,Tateiri S,Iida H

更新日期：1979-06-01 00:00:00

abstract：:Examination of Listeria monocytogenes prevalence among ready-to-eat foods in Japan revealed frequent (5.7 to 12.1%) contamination of minced tuna and fish roe products, and the isolates had the same virulence levels as clinical isolates in terms of invasion efficiency and infectivity in cell cultures and a murine infec...

journal_title：Applied and environmental microbiology

authors： Miya S,Takahashi H,Ishikawa T,Fujii T,Kimura B

更新日期：2010-05-01 00:00:00

abstract：:The sediment-water interface in Halifax Harbor supports a microbial population of 6.95 x 10 cells per g (dry weight). As determined by the standard technique of suspending subsamples in filtered seawater, the uptake of added glutamic acid by this population is 113.5 ng g (dry weight) h. An alternate technique was deve...

journal_title：Applied and environmental microbiology

authors： Novitsky JA

更新日期：1983-06-01 00:00:00

abstract：:Xylanase activity from naturally occurring color variants of Aureobasidium pullulans was associated with extracellular monomeric proteins of 20 to 21 kilodaltons. Xylanase represented nearly half the total extracellular protein, with a yield of up to 0.3 g of xylanase per liter. The specific activity of partially puri...

journal_title：Applied and environmental microbiology

authors： Leathers TD

更新日期：1986-11-01 00:00:00

abstract：UNLABELLED:Copper sulfate (CuSO4) has been widely used as an algicide to control harmful cyanobacterial blooms (CyanoHABs) in freshwater lakes. However, there are increasing concerns about this application, due mainly to the general toxicity of CuSO4 to other aquatic species and its long-term persistence in the environ...

journal_title：Applied and environmental microbiology

authors： Zhang BH,Ding ZG,Li HQ,Mou XZ,Zhang YQ,Yang JY,Zhou EM,Li WJ

更新日期：2016-08-15 00:00:00

abstract：:The determination of bacterial densities in aquatic sediments generally requires that a dilution-mixing treatment be used before enumeration of organisms by the most-probable-number fermentation tube method can be done. Differential sediment and organism settling rates may, however, influence the distribution of the m...

journal_title：Applied and environmental microbiology

authors： Doyle JD,Tunnicliff B,Kramer RE,Brickler SK

更新日期：1984-10-01 00:00:00