Color Variants of Aureobasidium pullulans Overproduce Xylanase with Extremely High Specific Activity.

abstract：:Our goal was to develop a field soil biodegradation assay using (13)C-labeled compounds and identify the active microorganisms by analyzing 16S rRNA genes in soil-derived (13)C-labeled DNA. Our biodegradation approach sought to minimize microbiological artifacts caused by physical and/or nutritional disturbance of soi...

journal_title：Applied and environmental microbiology

authors： Padmanabhan P,Padmanabhan S,DeRito C,Gray A,Gannon D,Snape JR,Tsai CS,Park W,Jeon C,Madsen EL

更新日期：2003-03-01 00:00:00

abstract：:The quantification of 16S rRNA by oligonucleotide probe hybridization was investigated with MagnaGraph (Micron Separation, Inc. [MSI]), Magna Charge (MSI), Magna (MSI), Immobilon-N (Millipore Corporation), and Nytran (Schleicher & Schuell, Inc.) membranes as supports for nucleic acid immobilization. The levels of dete...

journal_title：Applied and environmental microbiology

authors： Raskin L,Capman WC,Kane MD,Rittmann BE,Stahl DA

更新日期：1996-01-01 00:00:00

abstract：:Lantibiotics, such as nisin and subtilin, are lanthionine-containing peptides that exhibit antimicrobial as well as pheromone-like autoinducing activity. Autoinduction is specific for each lantibiotic, and reporter systems for nisin and subtilin autoinduction are available. In this report, we used the previously repor...

journal_title：Applied and environmental microbiology

authors： Fuchs SW,Jaskolla TW,Bochmann S,Kötter P,Wichelhaus T,Karas M,Stein T,Entian KD

更新日期：2011-03-01 00:00:00

abstract：:Enzymatic activities that could be involved in methanethiol generation in five cheese-ripening bacteria were assayed, and the major sulfur compounds produced were identified. L-Methionine and alpha-keto-gamma-methyl-thio-butyric acid demethiolating activities were detected in whole cells and cell extracts (CFEs) of al...

journal_title：Applied and environmental microbiology

authors： Bonnarme P,Psoni L,Spinnler HE

更新日期：2000-12-01 00:00:00

abstract：:We analyzed the communities of soil basidiomycetes in agroecosystems that differ in tillage history at the Kellogg Biological Station Long-Term Ecological Research site near Battle Creek, Michigan. The approach combined soil DNA extraction through a bead-beating method modified to increase recovery of fungal DNA, PCR ...

journal_title：Applied and environmental microbiology

authors： Lynch MD,Thorn RG

更新日期：2006-11-01 00:00:00

abstract：:Fermentation of the pentose sugar xylose to ethanol in lignocellulosic biomass would make bioethanol production economically more competitive. Saccharomyces cerevisiae, an efficient ethanol producer, can utilize xylose only when expressing the heterologous genes XYL1 (xylose reductase) and XYL2 (xylitol dehydrogenase)...

journal_title：Applied and environmental microbiology

authors： Johansson B,Christensson C,Hobley T,Hahn-Hägerdal B

更新日期：2001-09-01 00:00:00

abstract：:Ursodeoxycholic acid dissolves cholesterol gallstones in humans. In the present study optimum conditions for ursodeoxycholic acid production by Fusarium equiseti M41 were studied. Resting mycelia of F. equiseti M41 showed maximum conversion at 28 degrees C, pH 8.0, and dissolved oxygen tension of higher than 60% satur...

journal_title：Applied and environmental microbiology

authors： Kulprecha S,Ueda T,Nihira T,Yoshida T,Taguchi H

更新日期：1985-02-01 00:00:00

abstract：:There is growing interest in the application of human-associated fecal source identification quantitative real-time PCR (qPCR) technologies for water quality management. The transition from a research tool to a standardized protocol requires a high degree of confidence in data quality across laboratories. Data quality...

journal_title：Applied and environmental microbiology

authors： Shanks OC,Kelty CA,Oshiro R,Haugland RA,Madi T,Brooks L,Field KG,Sivaganesan M

更新日期：2016-04-18 00:00:00

abstract：:A 1,6-dichlorohexane-degrading strain of Acinetobacter sp. was isolated from activated sludge. The organism could grow with and quantitatively release halide from 1,6-dichlorohexane, 1,9-dichlorononane, 1-chloropentane, 1-chlorobutane, 1-bromopentane, ethylbromide, and 1-iodopropane. Crude extracts contained an induci...

journal_title：Applied and environmental microbiology

authors： Janssen DB,Jager D,Witholt B

更新日期：1987-03-01 00:00:00

abstract：:A method was developed to detect NO- or N(2)O-producing bacteria in solid or liquid medium by their ability to oxidize the redox indicator resazurin from its reduced colorless form to its oxidized pink form. The method was sensitive to as little as 35 nM N(2)O or 0.5 nM NO. Ninety-one percent of the colonies that oxid...

journal_title：Applied and environmental microbiology

authors： Jenneman GE,Montgomery AD,McInerney MJ

更新日期：1986-04-01 00:00:00

abstract：UNLABELLED:Copper sulfate (CuSO4) has been widely used as an algicide to control harmful cyanobacterial blooms (CyanoHABs) in freshwater lakes. However, there are increasing concerns about this application, due mainly to the general toxicity of CuSO4 to other aquatic species and its long-term persistence in the environ...

journal_title：Applied and environmental microbiology

authors： Zhang BH,Ding ZG,Li HQ,Mou XZ,Zhang YQ,Yang JY,Zhou EM,Li WJ

更新日期：2016-08-15 00:00:00

abstract：:In order to identify antifungal compounds from natural sources to be used as ingredients in the bakery industry, water/salt-soluble extracts (WSE) from different legume flour hydrolysates obtained by the use of a fungal protease were assayed against Penicillium roqueforti DPPMAF1. The agar diffusion assays allowed the...

journal_title：Applied and environmental microbiology

authors： Rizzello CG,Lavecchia A,Gramaglia V,Gobbetti M

更新日期：2015-06-15 00:00:00

abstract：:Members of the uncultivated bacterial division TM7 have been detected in the human mouth, but little information is available regarding their prevalence and diversity at this site. Human subgingival plaque samples from healthy sites and sites exhibiting various stages of periodontal disease were analyzed for the prese...

journal_title：Applied and environmental microbiology

authors： Brinig MM,Lepp PW,Ouverney CC,Armitage GC,Relman DA

更新日期：2003-03-01 00:00:00

abstract：:Transformed root cultures of three nonmycotrophic and one mycotrophic plant species stimulated germination and hyphal growth of the vesicular-arbuscular mycorrhizal fungus Glomus etunicatum (Becker & Gerd.) in a gel medium. However, only roots of the mycotrophic species (carrot) supported continued hyphal exploration ...

journal_title：Applied and environmental microbiology

authors： Schreiner RP,Koide RT

更新日期：1993-08-01 00:00:00

abstract：:The industrially important organism Corynebacterium glutamicum has been characterized in recent years for its robust ability to assimilate aromatic compounds. In this study, C. glutamicum strain AS 1.542 was investigated for its ability to catabolize phenylacetic acid (PAA). The paa genes were identified; they are org...

journal_title：Applied and environmental microbiology

authors： Chen X,Kohl TA,Rückert C,Rodionov DA,Li LH,Ding JY,Kalinowski J,Liu SJ

更新日期：2012-08-01 00:00:00

abstract：:Phages play a key role in the marine environment by regulating the transfer of energy between trophic levels and influencing global carbon and nutrient cycles. The diversity of marine phage communities remains difficult to characterize because of the lack of a signature gene common to all phages. Recent studies have d...

journal_title：Applied and environmental microbiology

authors： Goldsmith DB,Crosti G,Dwivedi B,McDaniel LD,Varsani A,Suttle CA,Weinbauer MG,Sandaa RA,Breitbart M

更新日期：2011-11-01 00:00:00

abstract：:In this study, the feasibility of fluorescence lifetime imaging (FLIM) for measurement of RNA:DNA ratios in microorganisms was assessed. The fluorescence lifetime of a nucleic acid-specific probe (SYTO 13) was used to directly measure the RNA:DNA ratio inside living bacterial cells. In vitro, SYTO 13 showed shorter fl...

journal_title：Applied and environmental microbiology

authors： Walczysko P,Kuhlicke U,Knappe S,Cordes C,Neu TR

更新日期：2008-01-01 00:00:00

abstract：:Time series incubations were conducted to provide estimates for the size selectivities and rates of protistan grazing that may be occurring in a sandy, contaminated aquifer. The experiments involved four size classes of fluorescently labeled groundwater bacteria (FLB) and 2- to 3-microns-long nanoflagellates, primaril...

journal_title：Applied and environmental microbiology

authors： Kinner NE,Harvey RW,Blakeslee K,Novarino G,Meeker LD

更新日期：1998-02-01 00:00:00

abstract：:Compared to freshwater and the open ocean, less is known about bacterioplankton community structure and spatiotemporal dynamics in estuaries, particularly those with long residence times. The Chesapeake Bay is the largest estuary in the United States, but despite its ecological and economic significance, little is kno...

journal_title：Applied and environmental microbiology

authors： Kan J,Suzuki MT,Wang K,Evans SE,Chen F

更新日期：2007-11-01 00:00:00

abstract：:An examination was made of various sites along the length of the swine large intestine, using strictly anaerobic culture methods. Sites were separated by differential washing into fractions described as lumenal content, lumenal surface layer, and intestinal wall tissue. Direct microscopic clump counts averaged 13.3 x ...

journal_title：Applied and environmental microbiology

authors： Russell EG

更新日期：1979-02-01 00:00:00

abstract：:The effect of long-term application of heavy metal-laden sewage sludge on the total heterotrophic aerobic and the cadmium-resistant soil bacterial communities was studied. Gram-positive bacteria were completely absent from resistant communities. These findings suggest that this group is highly susceptible to Cd. Shann...

journal_title：Applied and environmental microbiology

authors： Barkay T,Tripp SC,Olson BH

更新日期：1985-02-01 00:00:00

abstract：:This study investigated the effect of a 30-cm covering of finished compost (FC) on survival of Escherichia coli O157:H7 and Salmonella spp. in active static and windrow composting systems. Feedstocks inoculated with E. coli O157:H7 (7.41 log CFU/g) and Salmonella (6.46 log CFU/g) were placed in biosentry tubes (7.5-cm...

journal_title：Applied and environmental microbiology

authors： Patel JR,Yossa I,Macarisin D,Millner P

更新日期：2015-03-01 00:00:00

abstract：:Bacteria play important roles in mineral weathering and soil formation. However, few reports of mineral weathering bacteria inhabiting subsurfaces of soil profiles have been published, raising the question of whether the subsurface weathering bacteria are fundamentally distinct from those in surface communities. To ad...

journal_title：Applied and environmental microbiology

authors： Huang J,Sheng XF,Xi J,He LY,Huang Z,Wang Q,Zhang ZD

更新日期：2014-01-01 00:00:00

abstract：:Murine norovirus (MNV) was used as a surrogate to study resistance of human norovirus to disinfectants used in hospitals. MNV was sensitive to alcohol, alcohol hand rubs, bleach, and povidone iodine-based disinfectant. Real-time reverse transcription-PCR results indicated that the presence of viral RNA did not correla...

journal_title：Applied and environmental microbiology

authors： Belliot G,Lavaux A,Souihel D,Agnello D,Pothier P

更新日期：2008-05-01 00:00:00

abstract：:Methods to assess the impact of goose fecal contamination are needed as the result of the increasing number of Canada geese (Branta canadensis) near North American inland waters. However, there is little information on goose fecal microbial communities, and such data are important for the development of host-specific ...

journal_title：Applied and environmental microbiology

authors： Lu J,Santo Domingo JW,Hill S,Edge TA

更新日期：2009-09-01 00:00:00

abstract：:Of 98 suspected food-borne Clostridium perfringens isolates obtained from a nationwide survey by the Food and Consumer Product Safety Authority in The Netherlands, 59 strains were identified as C. perfringens type A. Using PCR-based techniques, the cpe gene encoding enterotoxin was detected in eight isolates, showing ...

journal_title：Applied and environmental microbiology

authors： Xiao Y,Wagendorp A,Moezelaar R,Abee T,Wells-Bennik MH

更新日期：2012-10-01 00:00:00

abstract：:The degradation of three polycyclic aromatic hydrocarbons (PAH), pyrene (PYR), benz[a]anthracene (BAA), and benzo[a]pyrene (BaP), by Mycobacterium sp. strain RJGII-135 was studied. The bacterium was isolated from an abandoned coal gasification site soil by analog enrichment techniques and found to mineralize [14C]PYR....

journal_title：Applied and environmental microbiology

authors： Schneider J,Grosser R,Jayasimhulu K,Xue W,Warshawsky D

更新日期：1996-01-01 00:00:00

abstract：:Infrared spectra of type cultures of the six recognized species of the genus Listeria and of Listeria grayi subsp. murrayi were recorded. By use of a library of 59 spectra, comprising at least six replicates of each type, discrimination by canonical variate analysis of the spectral amplitudes allowed all of the spectr...

journal_title：Applied and environmental microbiology

authors： Holt C,Hirst D,Sutherland A,MacDonald F

更新日期：1995-01-01 00:00:00

abstract：:Gas chromatography-mass spectrometry and nuclear magnetic resonance spectroscopy were used to study the metabolism of deuterated n-alkanes (C6 to C12) and 1-13C-labeled n-hexane by a highly enriched sulfate-reducing bacterial culture. All substrates were activated via fumarate addition to form the corresponding alkyls...

journal_title：Applied and environmental microbiology

authors： Davidova IA,Gieg LM,Nanny M,Kropp KG,Suflita JM

更新日期：2005-12-01 00:00:00

abstract：:A method has been described which can compare the efficacy of different nongermicidal handwashing protocols for removal of transient microbial flora without the necessity of establishing or relying on a previously determined baseline for an individual subject. The wash effluent is collected, and colony counts for the ...

journal_title：Applied and environmental microbiology

authors： Vesley D,Lillquist DR,Le CT

更新日期：1985-05-01 00:00:00