Use of murine norovirus as a surrogate to evaluate resistance of human norovirus to disinfectants.

abstract：:A pair of vectors for expression of heterologous genes in Lactococcus lactis was constructed. In addition to an origin of replication that has a broad host range, these vectors contain a multiple cloning site flanked by gene expression signals originating from L. lactis subsp. cremoris Wg2. The two vectors, about 3.7 ...

journal_title：Applied and environmental microbiology

authors： van de Guchte M,van der Vossen JM,Kok J,Venema G

更新日期：1989-01-01 00:00:00

abstract：:The existence of a hydrogen sulfide:ferric ion oxidoreductase, which catalyzes the oxidation of elemental sulfur with ferric ions as an electron acceptor to produce ferrous and sulfite ions, was assayed with washed intact cells and cell extracts of various kinds of iron-oxidizing bacteria, such as Thiobacillus ferroox...

journal_title：Applied and environmental microbiology

authors： Sugio T,White KJ,Shute E,Choate D,Blake RC

更新日期：1992-01-01 00:00:00

abstract：:Large numbers of bacteriophages (2 x 10(7) to 1 x 10(8)/ml) were present in ruminal fluid from sheep and cattle. Twenty-six distinct types were identified and placed in three morphological groups; several phages possessed unusual structural features. The large numbers and diversity of phages observed indicates a possi...

journal_title：Applied and environmental microbiology

authors： Klieve AV,Bauchop T

更新日期：1988-06-01 00:00:00

abstract：:We describe here a new enzyme-coupled assay for the quantitation of d-xylose using readily available enzymes that allows kinetic evaluation of hemicellulolytic enzymes using natural xylooligosaccharide substrates. Hydrogen peroxide is generated as an intermediary analyte, which allows flexibility in the choice of the ...

journal_title：Applied and environmental microbiology

authors： Wagschal K,Franqui-Espiet D,Lee CC,Robertson GH,Wong DW

更新日期：2005-09-01 00:00:00

abstract：:Toxin-producing isolates of Bacillus licheniformis were obtained from foods involved in food poisoning incidents, from raw milk, and from industrially produced baby food. The toxin detection method, based on the inhibition of boar spermatozoan motility, has been shown previously to be a sensitive assay for the emetic ...

journal_title：Applied and environmental microbiology

authors： Salkinoja-Salonen MS,Vuorio R,Andersson MA,Kämpfer P,Andersson MC,Honkanen-Buzalski T,Scoging AC

更新日期：1999-10-01 00:00:00

abstract：:There have been few reports on the epidemiological analysis of environmental Leptospira isolates. This is probably because the isolation of leptospires from the environment was usually unsuccessful due to the overgrowth of contaminants and the slow growth of Leptospira. In this study, we collected a total of 88 sample...

journal_title：Applied and environmental microbiology

authors： Saito M,Villanueva SY,Chakraborty A,Miyahara S,Segawa T,Asoh T,Ozuru R,Gloriani NG,Yanagihara Y,Yoshida S

更新日期：2013-01-01 00:00:00

abstract：:A novel technique, combining labelling and stereological methods, for the determination of spatial distribution of two microorganisms in a biofilm is presented. Cells of Nitrosomonas europaea (ATCC 19718) and Nitrobacter agilis (ATCC 14123) were homogeneously distributed in a kappa-carrageenan gel during immobilizatio...

journal_title：Applied and environmental microbiology

authors： Hunik JH,van den Hoogen MP,de Boer W,Smit M,Tramper J

更新日期：1993-06-01 00:00:00

abstract：:Mass spectral analysis of T-2 toxin formed during the growth of Fusarium sporotrichioides (ATCC 24043) in the presence of H(2)O showed incorporation of up to three O atoms per toxin molecule. The carbonyl oxygens of the acetates at C-4 and C-15 and of the isovalerate at C-8 were derived from H(2)O. Toxin formed in the...

journal_title：Applied and environmental microbiology

authors： Desjardins AE,Plattner RD,Vanmiddlesworth F

更新日期：1986-03-01 00:00:00

abstract：:In various sediments in which Fe(III) reduction was the terminal electron-accepting process, [C]glucose was fermented to C-fatty acids in a manner similar to that observed in methanogenic sediments. These results are consistent with the hypothesis that, in Fe(III)-reducing sediments, fermentable substrates are oxidize...

journal_title：Applied and environmental microbiology

authors： Lovley DR,Phillips EJ

更新日期：1989-12-01 00:00:00

abstract：:The vaginal mucosa of ovariectomized female rats has been examined by scanning electron microscopy before and after estrogen [1,3,5(10)-estratriene-3,17beta-diol] treatment. Without estrogen stimulation vaginal colonization is minimal and the epithelium is characterized by a layer of epithelial cells covered with smal...

journal_title：Applied and environmental microbiology

authors： Larsen B,Markovetz AJ,Galask RP

更新日期：1977-07-01 00:00:00

abstract：:A polymerase chain reaction (PCR) method designed to sensitively detect and identify Campylobacter jejuni and Campylobacter coli without the need for isolating and culturing strains is described. The intergenic sequence between the flagellin genes flaA and flaB was amplified and characterized with a triple primer or s...

journal_title：Applied and environmental microbiology

authors： Wegmüller B,Lüthy J,Candrian U

更新日期：1993-07-01 00:00:00

abstract：:In soil, Acidobacteria constitute on average 20% of all bacteria, are highly diverse, and are physiologically active in situ. However, their individual functions and interactions with higher taxa in soil are still unknown. Here, potential effects of land use, soil properties, plant diversity, and soil nanofauna on aci...

journal_title：Applied and environmental microbiology

authors： Naether A,Foesel BU,Naegele V,Wüst PK,Weinert J,Bonkowski M,Alt F,Oelmann Y,Polle A,Lohaus G,Gockel S,Hemp A,Kalko EK,Linsenmair KE,Pfeiffer S,Renner S,Schöning I,Weisser WW,Wells K,Fischer M,Overmann J,Friedric

更新日期：2012-10-01 00:00:00

abstract：:The accurate description of a microbial community is an important first step in understanding the roles of its components in ecosystem function. A method for surveying microbial communities termed serial analysis of rRNA genes (SARD) is described here. Through a series of molecular cloning steps, short DNA sequence ta...

journal_title：Applied and environmental microbiology

authors： Ashby MN,Rine J,Mongodin EF,Nelson KE,Dimster-Denk D

更新日期：2007-07-01 00:00:00

abstract：:We constructed a 7.9-kilobase-pair recombinant shuttle plasmid, designated pHR106, by combining desired segments of three plasmids: an Escherichia coli plasmid (pSL100) which provides a multiple cloning site, a Clostridium perfringens plasmid (pJU122) which provides a clostridial origin of replication, and an E. coli ...

journal_title：Applied and environmental microbiology

authors： Roberts I,Holmes WM,Hylemon PB

更新日期：1988-01-01 00:00:00

abstract：:A novel method of detecting extracellular protease activity at biofilm-substratum interfaces was developed. This method utilizes fluorescent molecules bound to cellulose substrata with a lectin. Extracellular proteases degrade the lectin and release the fluorochrome into solution. This new technique and a standard dis...

journal_title：Applied and environmental microbiology

authors： Francoeur SN,Wetzel RG,Neely RK

更新日期：2001-09-01 00:00:00

abstract：:Since 2006, Geomyces destructans, the causative agent of white nose syndrome (WNS), has killed over 5.7 million bats in North America. The current hypothesis suggests that this novel fungus is an invasive species from Europe, but little is known about the diversity within the genus Geomyces and its distribution on bat...

journal_title：Applied and environmental microbiology

authors： Johnson LJ,Miller AN,McCleery RA,McClanahan R,Kath JA,Lueschow S,Porras-Alfaro A

更新日期：2013-09-01 00:00:00

abstract：:The actions of cyclomaltodextrin glucanotransferases (CGTase; EC 2.4.1.19) from alkalophilic Bacillus sp. strain A2-5a (A2-5a CGTase), Bacillus macerans (Bmac CGTase), and Bacillus stearothermophilus (Bste CGTase) on amylose were investigated. All three enzymes produced large cyclic alpha-1,4-glucans (cycloamyloses) a...

journal_title：Applied and environmental microbiology

authors： Terada Y,Sanbe H,Takaha T,Kitahata S,Koizumi K,Okada S

更新日期：2001-04-01 00:00:00

abstract：:The interaction between Glomus intraradices and Pratylenchus coffeae on transformed carrot roots was studied in root organ culture. G. intraradices provided the roots with increased protection against P. coffeae by suppressing nematode reproduction in the roots. The internal and external mycorrhizal development was no...

journal_title：Applied and environmental microbiology

authors： Elsen A,Declerck S,De Waele D

更新日期：2003-07-01 00:00:00

abstract：:Two microorganisms which could degrade soybean lees efficiently were isolated and identified as Bacillus circulans and B. stearothermophilus. These two strains secreted thermostable proteases into the medium and could digest soybean lees rapidly and completely at 50 degrees C. Initially, the soybean lees were degraded...

journal_title：Applied and environmental microbiology

authors： Kubo M,Okajima J,Hasumi F

更新日期：1994-01-01 00:00:00

abstract：:Predictive models, based on environmental and water quality variables, have been used to improve the timeliness and accuracy of recreational water quality assessments, but their effectiveness has not been studied in inland waters. Sampling at eight inland recreational lakes in Ohio was done in order to investigate usi...

journal_title：Applied and environmental microbiology

authors： Francy DS,Stelzer EA,Duris JW,Brady AM,Harrison JH,Johnson HE,Ware MW

更新日期：2013-03-01 00:00:00

abstract：:In order to develop a rapid and specific detection test for bacteria in soil, we improved a method based on the polymerase chain reaction (PCR). Each step of the protocol, including direct lysis of cells, DNA purification, and PCR amplification, was optimized. To increase the efficiency of lysis, a step particularly c...

journal_title：Applied and environmental microbiology

authors： Picard C,Ponsonnet C,Paget E,Nesme X,Simonet P

更新日期：1992-09-01 00:00:00

abstract：:The presence of Yersinia ruckeri in a French fish farm was investigated. Y. ruckeri was isolated mainly from algae and sediment samples rather than from water. Twenty-two Y. ruckeri isolates were obtained, and three strains were distinguished by enterobacterial repetitive intergenic consensus PCR amplification. These ...

journal_title：Applied and environmental microbiology

authors： Coquet L,Cosette P,Quillet L,Petit F,Junter GA,Jouenne T

更新日期：2002-02-01 00:00:00

abstract：:The Pseudomonas putida KT2440 TOL upper pathway is repressed under nonlimiting conditions in cells growing in chemostat with succinate as a carbon source. We show that the ptsN gene product IIA(Ntr) participates in this repression. Crc, involved in yeast extract-dependent repression in batch cultures, did not influenc...

journal_title：Applied and environmental microbiology

authors： Aranda-Olmedo I,Marín P,Ramos JL,Marqués S

更新日期：2006-11-01 00:00:00

abstract：:The anaerobic bacterium Eubacterium lentum, a common constituent of the intestinal microflora, inactivates digoxin by reducing the unsaturated lactone ring. Reduction of the cardiac glycoside by growing cultures of E. lentum ATCC 25559 proceeded in a stereospecific manner, with the 20R-dihydrodigoxin constituting more...

journal_title：Applied and environmental microbiology

authors： Robertson LW,Chandrasekaran A,Reuning RH,Hui J,Rawal BD

更新日期：1986-06-01 00:00:00

abstract：:1,2,3-Trichloropropane (TCP) is a highly toxic and recalcitrant compound. Haloalkane dehalogenases are bacterial enzymes that catalyze the cleavage of a carbon-halogen bond in a wide range of organic halogenated compounds. Haloalkane dehalogenase LinB from Sphingobium japonicum UT26 has, for a long time, been consider...

journal_title：Applied and environmental microbiology

authors： Monincová M,Prokop Z,Vévodová J,Nagata Y,Damborsky J

更新日期：2007-03-01 00:00:00

abstract：:The genetic diversity of Borrelia burgdorferi sensu stricto, the agent of Lyme disease in North America, has consequences for the performance of serological diagnostic tests and disease severity. To investigate B. burgdorferi diversity in Canada, where Lyme disease is emerging, bacterial DNA in 309 infected adult Ixod...

journal_title：Applied and environmental microbiology

authors： Ogden NH,Margos G,Aanensen DM,Drebot MA,Feil EJ,Hanincová K,Schwartz I,Tyler S,Lindsay LR

更新日期：2011-05-01 00:00:00

abstract：:phi-0303 is a temperate bacteriophage isolated from Lactobacillus helveticus CNRZ 303 strain after mitomycin C induction. In this work, the gene coding for a lytic protein of this bacteriophage was cloned using a library of phi-0303 in Escherichia coli DH5alpha. The lytic activity was detected by its expression, using...

journal_title：Applied and environmental microbiology

authors： Deutsch SM,Guezenec S,Piot M,Foster S,Lortal S

更新日期：2004-01-01 00:00:00

abstract：:The volatile substances present in a eutrophic shallow lake were determined qualitatively and quantitatively during the growing season, allowing a first-time analysis of the dynamics of these compounds in a water body. The major compounds found were beta-cyclocitral, alpha-cyclocitral, beta-ionone, 1,3,3-trimethylcycl...

journal_title：Applied and environmental microbiology

authors： Jüttner F

更新日期：1984-04-01 00:00:00

abstract：:Inactivation of Bacillus cereus spores during cooling (10 degrees C/h) from 90 degrees C occurred in two phases. One phase occurred during cooling from 90 to 80 degrees C; the second occurred during cooling from 46 to 38 degrees C. In contrast, no inactivation occurred when spores were cooled from a maximum temperatur...

journal_title：Applied and environmental microbiology

authors： Johnson KM,Busta FF

更新日期：1984-04-01 00:00:00

abstract：:A method was developed to follow bacterial nitrate reduction in freshwater sediments by using common high-performance liquid chromatographic equipment. The low detection limit (14 pmol) of the method enabled us to study concentration profiles and reaction kinetics under natural conditions. Significant nitrate concentr...

journal_title：Applied and environmental microbiology

authors： Hordijk CA,Snieder M,van Engelen JJ,Cappenberg TE

更新日期：1987-02-01 00:00:00