Estimation of bacterial nitrate reduction rates at in situ concentrations in freshwater sediments.

abstract：:This paper introduces a novel method for sampling pathogens in natural environments. It uses fabric boot socks worn over walkers' shoes to allow the collection of composite samples over large areas. Wide-area sampling is better suited to studies focusing on human exposure to pathogens (e.g., recreational walking). Thi...

journal_title：Applied and environmental microbiology

authors： Jones NR,Millman C,van der Es M,Hukelova M,Forbes KJ,Glover C,Haldenby S,Hunter PR,Jackson K,O'Brien SJ,Rigby D,Strachan NJC,Williams N,Lake IR,ENIGMA Consortium.

更新日期：2017-06-30 00:00:00

abstract：:Some effects of visible light on the survival of Escherichia coli in waters of the Butrón river were studied by comparing illuminated and nonilluminated systems. The following count methods were used: CFU on a selective medium (eosin-methylene blue agar), CFU on a medium of recuperation (Trypticase soy agar with yeast...

journal_title：Applied and environmental microbiology

authors： Barcina I,González JM,Iriberri J,Egea L

更新日期：1989-01-01 00:00:00

abstract：:Malonyl-coenzyme A (CoA) decarboxylase, malonyl-CoA synthetase, and malonate transporter mutants of Rhizobium leguminosarum bv. viciae and trifolii fixed N2 at wild-type rates on pea and clover, respectively. Thus, malonate does not drive N2 fixation in legume nodules. ...

journal_title：Applied and environmental microbiology

authors： Karunakaran R,East AK,Poole PS

更新日期：2013-07-01 00:00:00

abstract：:A methanol-utilizing bacterium, Protaminobacter ruber, produced a large amount of vitamin B(12). The compounds were isolated from the cells and identified as methylcobalamin (methyl-B(12)) and adenosylcobalamin (adenosyl-B(12)) by various tests. The variation in the form of B(12) during cultivation was examined by bio...

journal_title：Applied and environmental microbiology

authors： Sato K,Ueda S,Shimizu S

更新日期：1977-03-01 00:00:00

abstract：:Chromosomal integration of heterologous metabolic pathways is optimal for industrially relevant fermentation, as plasmid-based fermentation causes extra metabolic burden and genetic instabilities. In this work, chromosomal integration was adapted for the production of mevalonate, which can be readily converted into β-...

journal_title：Applied and environmental microbiology

authors： Wang J,Niyompanich S,Tai YS,Wang J,Bai W,Mahida P,Gao T,Zhang K

更新日期：2016-11-21 00:00:00

abstract：:When a combination of hydrogen peroxide and hypochlorite was used to surface sterilize rice seeds, a 10(2)- to 10(4)-fold decrease in CFU was observed during the first 15 h after inoculation of the rice rhizosphere organism Burkholderia vietnamiensis TVV75. This artifact could not be eliminated simply by rinsing the s...

journal_title：Applied and environmental microbiology

authors： Miché L,Balandreau J

更新日期：2001-07-01 00:00:00

abstract：:Accumulating evidence suggests that pesticides have played a role in the increased rate of honey bee colony loss. One of the most commonly used pesticides in the United States is the neonicotinoid imidacloprid. Although the primary mode of action of imidacloprid is on the insect nervous system, it has also been shown ...

journal_title：Applied and environmental microbiology

authors： Raymann K,Motta EVS,Girard C,Riddington IM,Dinser JA,Moran NA

更新日期：2018-06-18 00:00:00

abstract：:A theoretical analysis of the detachment of bacteria adhering to substratum surfaces upon the passage of an air-liquid interface is given, together with experimental results for bacterial detachment in the absence and presence of a conditioning film on different substratum surfaces. Bacteria (Streptococcus sobrinus HG...

journal_title：Applied and environmental microbiology

authors： Gómez-Suárez C,Busscher HJ,van der Mei HC

更新日期：2001-06-01 00:00:00

abstract：:In Vibrio vulnificus, virulence for eels is associated with serovar E strains. In this study, we investigated some biological properties of purified lipopolysaccharides (LPSs) from serovar E and non-serovar E strains. Purified LPSs retained their O-polysaccharidic side chains and did not show any differences that coul...

journal_title：Applied and environmental microbiology

authors： Biosca EG,Collado RM,Oliver JD,Amaro C

更新日期：1999-02-01 00:00:00

abstract：:The ability of yeasts to ferment cellodextrins is rare. Candida wickerhamii is able to use these sugars for alcohol production because of a cell-bound, extracellular, beta-glucosidase that is unusual by not being inhibited by glucose. A cDNA expression library in lambda phage was prepared with mRNA isolated from cello...

journal_title：Applied and environmental microbiology

authors： Skory CD,Freer SN

更新日期：1995-02-01 00:00:00

abstract：:The plant-pathogenic fungus Fusarium oxysporum was successfully transformed with the beta-D-glucuronidase gene from Escherichia coli (gusA) (GUS system) in combination with the gene for nitrate reductase (niaD) as the selectable marker. The frequency of cotransformation, as determined by GUS expression on plates conta...

journal_title：Applied and environmental microbiology

authors： Couteaudier Y,Daboussi MJ,Eparvier A,Langin T,Orcival J

更新日期：1993-06-01 00:00:00

abstract：:DNA probes that are specific for each of five predominant species of human colonic Bacteroides (B. thetaiotaomicron, B. uniformis, B. distasonis, "Bacteroides group 3452-A", and B. ovatus) were used to detect and enumerate these species in fecal samples from two adult volunteers. These five species are capable of ferm...

journal_title：Applied and environmental microbiology

authors： Kuritza AP,Shaughnessy P,Salyers AA

更新日期：1986-02-01 00:00:00

abstract：:The diversity of deep-sea high-pressure-adapted (piezophilic) microbes in isolated monoculture remains low. In this study, a novel obligately psychropiezophilic bacterium was isolated from seawater collected from the Puerto Rico Trench at a depth of ∼6,000 m. This isolate, designated YC-1, grew best in a nutrient-rich...

journal_title：Applied and environmental microbiology

authors： Cao Y,Chastain RA,Eloe EA,Nogi Y,Kato C,Bartlett DH

更新日期：2014-01-01 00:00:00

abstract：:The expanded Fermi solution was originally developed for estimating the number of food-poisoning victims when information concerning the circumstances of exposure is scarce. The method has been modified for estimating the initial number of pathogenic or probiotic cells or spores so that enough of them will survive the...

journal_title：Applied and environmental microbiology

authors： Peleg M,Normand MD,Horowitz J,Corradini MG

更新日期：2011-01-01 00:00:00

abstract：:Listeria monocytogenes is a food-borne, opportunistic, bacterial pathogen causing a wide spectrum of diseases, including meningitis, septicemia, abortion, and gastroenteritis, in humans and animals. Among the 13 L. monocytogenes serovars described, human listeriosis is mostly associated with strains of serovars 4b, 1/...

journal_title：Applied and environmental microbiology

authors： Severino P,Dussurget O,Vêncio RZ,Dumas E,Garrido P,Padilla G,Piveteau P,Lemaître JP,Kunst F,Glaser P,Buchrieser C

更新日期：2007-10-01 00:00:00

abstract：:Saccharomyces cerevisiae normally cannot assimilate mannitol, a promising brown macroalgal carbon source for bioethanol production. The molecular basis of this inability remains unknown. We found that cells capable of assimilating mannitol arose spontaneously from wild-type S. cerevisiae during prolonged culture in ma...

journal_title：Applied and environmental microbiology

authors： Chujo M,Yoshida S,Ota A,Murata K,Kawai S

更新日期：2015-01-01 00:00:00

abstract：:Addition of sodium isoascorbate to the formulation for perishable canned comminuted cured meat markedly enhanced the efficacy of nitrite against Clostridium botulinum. This effect was reproducible through a series of three tests. In one test it was found that the initial addition of 50 microgram of sodium nitrite per ...

journal_title：Applied and environmental microbiology

authors： Tompkin RB,Christiansen LN,Shaparis AB

更新日期：1978-01-01 00:00:00

abstract：:Accurate enumeration of viruses within environmental samples is critical for investigations of the ecological role of viruses and viral infection within microbial communities. This report evaluates differences in viral and bacterial direct counts between estuarine sediment samples which were either immediately process...

journal_title：Applied and environmental microbiology

authors： Helton RR,Liu L,Wommack KE

更新日期：2006-07-01 00:00:00

abstract：:Yarrowia lipolytica produces brown extracellular pigments that correlate with tyrosine catabolism. During tyrosine depletion, the yeast accumulated homogentisic acid, p-hydroxyphenylethanol, and p-hydroxyphenylacetic acid in the medium. Homogentisic acid accumulated under all aeration conditions tested, but its concen...

journal_title：Applied and environmental microbiology

authors： Carreira A,Ferreira LM,Loureiro V

更新日期：2001-08-01 00:00:00

abstract：:Lactocin 705 is a bacteriocin whose activity depends upon the complementation of two peptides, termed Lac705alpha and Lac705beta. Neither Lac705alpha nor Lac705beta displayed bacteriocin activity by itself when the growth of sensitive cells was monitored. To obtain molecular insights into the lactocin 705 mechanism of...

journal_title：Applied and environmental microbiology

authors： Castellano P,Vignolo G,Farías RN,Arrondo JL,Chehín R

更新日期：2007-01-01 00:00:00

abstract：:Examination of cucumber roots (Cucumis sativus L.) grown in bark compost media and of the surrounding edaphic substrate showed profiles of polar lipid fatty acids commonly found in bacteria. The composition of fatty acids in these profiles differed significantly between roots grown in a medium naturally suppressive to...

journal_title：Applied and environmental microbiology

authors： Tunlid A,Hoitink HA,Low C,White DC

更新日期：1989-06-01 00:00:00

abstract：:Laboratory strains of Saccharomyces cerevisiae have been widely used as a model for studying eukaryotic cells and mapping the molecular mechanisms of many different human diseases. Industrial wine yeasts, on the other hand, have been selected on the basis of their adaptation to stringent environmental conditions and t...

journal_title：Applied and environmental microbiology

authors： Pizarro FJ,Jewett MC,Nielsen J,Agosin E

更新日期：2008-10-01 00:00:00

abstract：:A multiplex fluorogenic PCR assay for simultaneous detection of pathogenic Salmonella strains and Escherichia coli O157:H7 was developed and evaluated for use in detecting very low levels of these pathogens in meat and feces. Two sets of primers were used to amplify a junctional segment of virulence genes sipB and sip...

journal_title：Applied and environmental microbiology

authors： Sharma VK,Carlson SA

更新日期：2000-12-01 00:00:00

abstract：:Factors affecting natural transformation of Acinetobacter calcoaceticus BD413 with homologous chromosomal DNA in a silt loam soil microcosm were investigated. Inducible transformation of declining populations of noncompetent A. calcoaceticus cells was detectable for up to 6 days when a simple carbon source, salts, and...

journal_title：Applied and environmental microbiology

authors： Nielsen KM,Bones AM,Van Elsas JD

更新日期：1997-10-01 00:00:00

abstract：:Short peptides of 11 residues were synthesized and tested against the economically important plant pathogenic bacteria Erwinia amylovora, Pseudomonas syringae, and Xanthomonas vesicatoria and compared to the previously described peptide Pep3 (WKLFKKILKVL-NH(2)). The antimicrobial activity of Pep3 and 22 analogues was ...

journal_title：Applied and environmental microbiology

authors： Ferre R,Badosa E,Feliu L,Planas M,Montesinos E,Bardají E

更新日期：2006-05-01 00:00:00

abstract：:The active population of low-affinity methanotrophs in a peat soil microcosm was characterized by stable-isotope probing. "Heavy" (13)C-labeled DNA, produced after microbial growth on (13)CH(4), was separated from naturally abundant (12)C-DNA by cesium chloride density gradient centrifugation and used as a template fo...

journal_title：Applied and environmental microbiology

authors： Morris SA,Radajewski S,Willison TW,Murrell JC

更新日期：2002-03-01 00:00:00

abstract：:The aim of this work was to develop a strategy to isolate a morphologically stable mutant of Streptomyces ambofaciens ATCC 15154 which produced high titers of spiramycin. The rationale was to grow a nitrosoguanidine-mutated population for many generations under nonselective conditions followed by two cycles of protopl...

journal_title：Applied and environmental microbiology

authors： Ford LM,Eaton TE,Godfrey OW

更新日期：1990-11-01 00:00:00

abstract：:Source attribution studies report that the consumption of contaminated poultry is the primary source for acquiring human campylobacteriosis. Oral administration of an engineered Escherichia coli strain expressing the Campylobacter jejuni N-glycan reduces bacterial colonization in specific-pathogen-free leghorn chicken...

journal_title：Applied and environmental microbiology

authors： Nothaft H,Perez-Muñoz ME,Gouveia GJ,Duar RM,Wanford JJ,Lango-Scholey L,Panagos CG,Srithayakumar V,Plastow GS,Coros C,Bayliss CD,Edison AS,Walter J,Szymanski CM

更新日期：2017-11-16 00:00:00

abstract：:Previous studies have shown that the cellobiohydrolases of the white rot basidiomycete Phanerochaete chrysosporium are encoded by a family of structurally related genes. In this investigation, we identified and sequenced the most highly transcribed gene, cbh1-4. Evidence suggests that in this fungus the dominant isozy...

journal_title：Applied and environmental microbiology

authors： Vanden Wymelenberg A,Covert S,Cullen D

更新日期：1993-10-01 00:00:00

abstract：:Protease-negative variants were shown to outcompete the wild-type strains of Streptococcus cremoris E(8), HP, and Wg(2) at pH values higher than 6.0 in milk. For S. cremoris E(8) this process was studied in more detail. At lower pH values the wild type had a selective advantage. This pH-dependent selection was not fou...

journal_title：Applied and environmental microbiology

authors： Hugenholtz J,Splint R,Konings WN,Veldkamp H

更新日期：1987-02-01 00:00:00