Construction of a lactococcal expression vector: expression of hen egg white lysozyme in Lactococcus lactis subsp. lactis.

abstract：:Studies have shown that certain opportunistic pathogenic species of nontuberculous mycobacteria (NTM) can be present in distributed drinking water. However, detailed information about NTM population composition in drinking water is lacking. Therefore, NTM communities in unchlorinated drinking water from the distributi...

journal_title：Applied and environmental microbiology

authors： van der Wielen PW,Heijnen L,van der Kooij D

更新日期：2013-10-01 00:00:00

abstract：:The siderophore synthetic system in Shewanella species is able to synthesize dozens of macrocyclic siderophores in vitro with synthetic precursors. In vivo, however, although three siderophores are produced naturally in Shewanella algae B516, which carries a lysine decarboxylase (AvbA) specific for siderophore synthes...

journal_title：Applied and environmental microbiology

authors： Wang S,Liang H,Liu L,Jiang X,Wu S,Gao H

更新日期：2020-03-18 00:00:00

abstract：:Microcosm experiments were conducted with soils contaminated with heavy metals (Pb and Cr) and aromatic hydrocarbons to determine the effects of each upon microbial community structure and function. Organic substrates were added as a driving force for change in the microbial community. Glucose represented an energy so...

journal_title：Applied and environmental microbiology

authors： Nakatsu CH,Carmosini N,Baldwin B,Beasley F,Kourtev P,Konopka A

更新日期：2005-12-01 00:00:00

abstract：:To understand phage infection and host cell lysis mechanisms in pathogenic Salmonella, a novel Salmonella enterica serovar Typhimurium-targeting bacteriophage, SPN9CC, belonging to the Podoviridae family was isolated and characterized. The phage infects S. Typhimurium via the O antigen of lipopolysaccharide (LPS) and ...

journal_title：Applied and environmental microbiology

authors： Shin H,Lee JH,Yoon H,Kang DH,Ryu S

更新日期：2014-01-01 00:00:00

abstract：:Strain SR, a monensin-sensitive, ammonia-producing ruminal bacterium, grew rapidly on arginine and lysine, but only if sodium was present. Arginine transport could be driven by either an electrical potential or a chemical gradient of sodium. Arginine was converted to ornithine, and it appeared that ornithine efflux cr...

journal_title：Applied and environmental microbiology

authors： Van Kessel JS,Russell JB

更新日期：1992-03-01 00:00:00

abstract：:The aim of this study was to investigate the effects on the cell membranes of Escherichia coli of 2.45-GHz microwave (MW) treatment under various conditions with an average temperature of the cell suspension maintained at 37°C in order to examine the possible thermal versus nonthermal effects of short-duration MW expo...

journal_title：Applied and environmental microbiology

authors： Rougier C,Prorot A,Chazal P,Leveque P,Leprat P

更新日期：2014-08-01 00:00:00

abstract：:The developing intestinal microbiota of breast-fed infants is considered to play an important role in the priming of the infants' mucosal and systemic immunity. Generally, Bifidobacterium and Lactobacillus predominate the microbiota of breast-fed infants. In intervention trials it has been shown that lactobacilli can ...

journal_title：Applied and environmental microbiology

authors： Haarman M,Knol J

更新日期：2006-04-01 00:00:00

abstract：:Here, we report simultaneous surface profile measurements of several bacterial species involved in microbially influenced corrosion and their solid-surface interfaces by using vertical scanning interferometry. The capacity to nondestructively quantify microscale topographic changes beneath a single bacterium without i...

journal_title：Applied and environmental microbiology

authors： Waters MS,El-Naggar MY,Hsu L,Sturm CA,Luttge A,Udwadia FE,Cvitkovitch DG,Goodman SD,Nealson KH

更新日期：2009-03-01 00:00:00

abstract：:The milk supply is considered a primary route for a bioterrorism attack with Bacillus anthracis spores because typical high-temperature short-time (HTST) pasteurization conditions cannot inactivate spores. In the event of intentional contamination, an effective method to inactivate the spores in milk under HTST proces...

journal_title：Applied and environmental microbiology

authors： Xu S,Labuza TP,Diez-Gonzalez F

更新日期：2008-06-01 00:00:00

abstract：:This work presents data on the application of a bacterial luciferase used to monitor gene expression of Streptococcus thermophilus in the digestive tract. The main result is that the bacterium was able to produce an active beta-galactosidase in the digestive tract, although it did not multiply during its transit. This...

journal_title：Applied and environmental microbiology

authors： Drouault S,Anba J,Corthier G

更新日期：2002-02-01 00:00:00

abstract：:A culture-independent method called mRNA profiling has been developed for the analysis of fecal enterobacteria and their physiological status in environmental samples. This taxon-specific approach determines the single-cell content of selected gene transcripts whose abundance is either directly or inversely proportion...

journal_title：Applied and environmental microbiology

authors： Chen H,Ponniah G,Salonen N,Blum P

更新日期：2004-08-01 00:00:00

abstract：:A molecular diagnostic system using single nucleotide polymorphisms (SNPs) was developed to identify four Sclerotinia species: S. sclerotiorum (Lib.) de Bary, S. minor Jagger, S. trifoliorum Erikss., and the undescribed species Sclerotinia species 1. DNAs of samples are hybridized with each of five 15-bp oligonucleoti...

journal_title：Applied and environmental microbiology

authors： Andrew M,Kohn LM

更新日期：2009-09-01 00:00:00

abstract：:The ability of yeasts to ferment cellodextrins is rare. Candida wickerhamii is able to use these sugars for alcohol production because of a cell-bound, extracellular, beta-glucosidase that is unusual by not being inhibited by glucose. A cDNA expression library in lambda phage was prepared with mRNA isolated from cello...

journal_title：Applied and environmental microbiology

authors： Skory CD,Freer SN

更新日期：1995-02-01 00:00:00

abstract：:To enhance food safety and stability, the food industry tends to use natural antimicrobials such as plant-derived compounds as an attractive alternative to chemical preservatives. Nonetheless, caution must be exercised in light of the potential for bacterial adaptation to these molecules, a phenomenon previously obser...

journal_title：Applied and environmental microbiology

authors： Dubois-Brissonnet F,Naïtali M,Mafu AA,Briandet R

更新日期：2011-02-01 00:00:00

abstract：:Vortex flow filtration (VFF) was used to concentrate viruses and dissolved DNA from freshwater and seawater samples taken in Florida, the Gulf of Mexico, and the Bahamas Bank. Recoveries of T2 phage and calf thymus DNA added to artificial seawater and concentrated by VFF were 72.8 and 80%, respectively. Virus concentr...

journal_title：Applied and environmental microbiology

authors： Paul JH,Jiang SC,Rose JB

更新日期：1991-08-01 00:00:00

abstract：:The effects of preexposure of soybean (Glycine max L. Merrill) roots to Rhizobium japonicum strains and subsequent establishment of other strains in the nodules were investigated by using combinations of effective strains (USDA 110 and USDA 138) and effective-ineffective strains (USDA 110 and SM-5). Strain USDA 110 wa...

journal_title：Applied and environmental microbiology

authors： Kosslak RM,Bohlool BB,Dowdle S,Sadowsky MJ

更新日期：1983-10-01 00:00:00

abstract：:The assembly of six genes that encode enzymes from glutamate-fermenting bacteria converted Escherichia coli into a glutaconate producer when grown anaerobically on a complex medium. The new anaerobic pathway starts with 2-oxoglutarate from general metabolism and proceeds via (R)-2-hydroxyglutarate, (R)-2-hydroxyglutar...

journal_title：Applied and environmental microbiology

authors： Djurdjevic I,Zelder O,Buckel W

更新日期：2011-01-01 00:00:00

abstract：:In this study, the feasibility of fluorescence lifetime imaging (FLIM) for measurement of RNA:DNA ratios in microorganisms was assessed. The fluorescence lifetime of a nucleic acid-specific probe (SYTO 13) was used to directly measure the RNA:DNA ratio inside living bacterial cells. In vitro, SYTO 13 showed shorter fl...

journal_title：Applied and environmental microbiology

authors： Walczysko P,Kuhlicke U,Knappe S,Cordes C,Neu TR

更新日期：2008-01-01 00:00:00

abstract：:Swarming Proteus spp. were detected with the use of proteometry (a most-probable-number technique) in the fecal material of selected animal species and in raw sewage from a local sewage treatment plant. Proteus spp. were not detected in any of several soil and freshwater samples examined. Since rat feces harbored high...

journal_title：Applied and environmental microbiology

authors： Stahl ML,Williams FD

更新日期：1981-03-01 00:00:00

abstract：:We report the isolation and characterization of a phototrophic ferrous iron [Fe(II)]-oxidizing bacterium named TIE-1 that differs from other Fe(II)-oxidizing phototrophs in that it is genetically tractable. Under anaerobic conditions, TIE-1 grows photoautotrophically with Fe(II), H2, or thiosulfate as the electron don...

journal_title：Applied and environmental microbiology

authors： Jiao Y,Kappler A,Croal LR,Newman DK

更新日期：2005-08-01 00:00:00

abstract：:In Agrobacterium tumefaciens, the balance between acquiring enough iron and avoiding iron-induced toxicity is regulated in part by Fur (ferric uptake regulator). A fur mutant was constructed to address the physiological role of the regulator. Atypically, the mutant did not show alterations in the levels of siderophore...

journal_title：Applied and environmental microbiology

authors： Kitphati W,Ngok-Ngam P,Suwanmaneerat S,Sukchawalit R,Mongkolsuk S

更新日期：2007-08-01 00:00:00

abstract：:Four hitherto undescribed Clostridium strains capable of cleaving the C ring of quercetin, kaempferol, and naringenin at C-3-C-4 were isolated from the fecal flora of humans. None of the strains cleaved catechin. C-ring fission occurred when the substrate was either in solution or in suspension. Mixed cultures of flav...

journal_title：Applied and environmental microbiology

authors： Winter J,Moore LH,Dowell VR Jr,Bokkenheuser VD

更新日期：1989-05-01 00:00:00

abstract：:Salmonella spp. are remarkably adaptable pathogens, and this adaptability allows these bacteria to thrive in a variety of environments and hosts. The mechanisms with which these pathogens establish within a niche amid the native microbiota remain poorly understood. Here, we aimed to uncover the mechanisms that enable ...

journal_title：Applied and environmental microbiology

authors： George AS,Cox CE,Desai P,Porwollik S,Chu W,de Moraes MH,McClelland M,Brandl MT,Teplitski M

更新日期：2018-02-14 00:00:00

abstract：:Due to concerns about a link between variant Creutzfeldt-Jakob disease in humans and similar prion protein-induced disease in cattle, i.e., bovine spongiform encephalopathy (BSE), strict controls are in place to exclude BSE-positive animals and/or specified risk materials including bovine central nervous system (CNS) ...

journal_title：Applied and environmental microbiology

authors： Daly DJ,Prendergast DM,Sheridan JJ,Blair IS,McDowell DA

更新日期：2002-02-01 00:00:00

abstract：:The preparation of DNA by alcohol precipitation in the presence of Na1 was used to enable the direct detection of Listeria monocytogenes in the amount of 10(3) CFU/0.5 g of sample. This procedure produces PCR-quality DNA directly from foods, such as soft cheese and minced meat. ...

journal_title：Applied and environmental microbiology

authors： Makino S,Okada Y,Maruyama T

更新日期：1995-10-01 00:00:00

abstract：:Increased listerial barotolerance at elevated osmolarity is attributed, in part, to the presence of accumulated betaine and L-carnitine. The percentage of listerial survival following exposure to 400 MPa for 5 min increased from 0.008 to 0.02% with added L-carnitine (5 mM) and to 0.05% with added betaine (5 mM). Furth...

journal_title：Applied and environmental microbiology

authors： Smiddy M,Sleator RD,Patterson MF,Hill C,Kelly AL

更新日期：2004-12-01 00:00:00

abstract：:In this study, food samples were intentionally contaminated with Escherichia coli O157:H7, and then DNA was isolated by using four commercial kits. The isolated DNA samples were compared by using real-time PCR detection of the Shiga toxin genes. The four kits tested worked similarly. ...

journal_title：Applied and environmental microbiology

authors： Heller LC,Davis CR,Peak KK,Wingfield D,Cannons AC,Amuso PT,Cattani J

更新日期：2003-03-01 00:00:00

abstract：:Correlations between the biomass of phytoplankton and the biomass of bacteria and between the biomass of bacteria and the biomass of protozoans suggest that there is coupling between these compartments of the "microbial loop." To investigate this coupling on the species level, bacteria and protozoans from untreated la...

journal_title：Applied and environmental microbiology

authors： van Hannen EJ,Mooij W,van Agterveld MP,Gons HJ,Laanbroek HJ

更新日期：1999-06-01 00:00:00

abstract：:When microbes lack the nutrients necessary for growth, they enter stationary phase. In cases when energy sources are still present in the environment, they must decide whether to continue to use their metabolic program to harvest the available energy. Here we characterized the metabolic response to a variety of types ...

journal_title：Applied and environmental microbiology

authors： Chubukov V,Sauer U

更新日期：2014-05-01 00:00:00

abstract：:Azospirillum lipoferum 4T has original properties such as nonmotility, melanin synthesis, and laccase activity. Following random Tn5 mutagenesis in A. lipoferum 4T, we obtained 10 mutants which were affected in melanization and laccase activity. The class 1 mutants, with intermediate levels of laccase activity, showed...

journal_title：Applied and environmental microbiology

authors： Faure D,Bouillant ML,Bally R

更新日期：1994-09-01 00:00:00