Culture-independent analysis of fecal enterobacteria in environmental samples by single-cell mRNA profiling.

abstract：:The preparation of DNA by alcohol precipitation in the presence of Na1 was used to enable the direct detection of Listeria monocytogenes in the amount of 10(3) CFU/0.5 g of sample. This procedure produces PCR-quality DNA directly from foods, such as soft cheese and minced meat. ...

journal_title：Applied and environmental microbiology

authors： Makino S,Okada Y,Maruyama T

更新日期：1995-10-01 00:00:00

abstract：:The alpha-galactosidase gene (aga) and a gene coding for a putative transcriptional regulator from the LacI/GalR family (galR) of Lactococcus raffinolactis ATCC 43920 were cloned and sequenced. When transferred into Lactococcus lactis and Pediococcus acidilactici strains, aga modified the sugar fermentation profile of...

journal_title：Applied and environmental microbiology

authors： Boucher I,Parrot M,Gaudreau H,Champagne CP,Vadeboncoeur C,Moineau S

更新日期：2002-12-01 00:00:00

abstract：:Many food-grade bacteria produce exopolysaccharides (EPS) that affect the texture of fermented food products and that may be involved in probiotic properties. Propionibacterium freudenreichii is a Gram-positive food-grade bacterium with reported probiotic capabilities that is widely used as starter in Swiss-type chees...

journal_title：Applied and environmental microbiology

authors： Deutsch SM,Le Bivic P,Hervé C,Madec MN,LaPointe G,Jan G,Le Loir Y,Falentin H

更新日期：2010-05-01 00:00:00

abstract：:Specific Saccharomyces cerevisiae strains were recently found to be capable of efficiently utilizing inulin, but genetic mechanisms of inulin hydrolysis in yeast remain unknown. Here we report functional characteristics of invertase SUC2 from strain JZ1C and demonstrate that SUC2 is the key enzyme responsible for inul...

journal_title：Applied and environmental microbiology

authors： Wang SA,Li FL

更新日期：2013-01-01 00:00:00

abstract：:Previous studies have demonstrated that Staphylococcus epidermidis isolates colonizing the skin of healthy humans do not typically encode icaADBC, the genes responsible for the production of polysaccharide intercellular adhesin or biofilms. It was therefore hypothesized that the presence of icaADBC was deleterious to ...

journal_title：Applied and environmental microbiology

authors： Rogers KL,Rupp ME,Fey PD

更新日期：2008-10-01 00:00:00

abstract：:We studied the role of the regulatory gene aflR and its product, AflR, in the biosynthesis of aflatoxin in Aspergillus. Western blot and enzyme-linked immunosorbent assay analyses revealed that aflatoxin B1 accumulation was directly related to AflR expression and was regulated by various environmental and nutritional ...

journal_title：Applied and environmental microbiology

authors： Liu BH,Chu FS

更新日期：1998-10-01 00:00:00

abstract：:A new method is described in which the Salkowski reaction is used for the rapid selection of alkaloid-producing mutants of the ergot fungus. This method was used to investigate the influence of a second mutation with N-methyl-N'-nitro-N-nitrosoguanidine (NTG) on various mutants selected by a preliminary NTG mutation o...

journal_title：Applied and environmental microbiology

authors： Srikrai S,Robbers JE

更新日期：1983-04-01 00:00:00

abstract：:The strain Burkholderia cepacia G4 aerobically mineralized trichloroethene (TCE) to CO(2) over a time period of approximately 20 h. Three biodegradation experiments were conducted with different bacterial optical densities at 540 nm (OD(540)s) in order to test whether isotope fractionation was consistent. The resultin...

journal_title：Applied and environmental microbiology

authors： Barth JA,Slater G,Schüth C,Bill M,Downey A,Larkin M,Kalin RM

更新日期：2002-04-01 00:00:00

abstract：:A negative enrichment procedure was developed which was capable of isolating Legionella pneumophila directly from seeded air-conditioning cooling tower water onto laboratory media. This procedure was based on an 8-h incubation under conditions that were bactericidal to the indigenous water microflora but merely bacter...

journal_title：Applied and environmental microbiology

authors： Thorpe TC,Miller RD

更新日期：1980-10-01 00:00:00

abstract：:Wild-type Bacillus subtilis ferments 20 g/liter glucose in 48 h, producing lactate and butanediol, but not ethanol or acetate. To construct an ethanologenic B. subtilis strain, homologous recombination was used to disrupt the native lactate dehydrogenase (LDH) gene (ldh) by chromosomal insertion of the Zymomonas mobil...

journal_title：Applied and environmental microbiology

authors： Romero S,Merino E,Bolívar F,Gosset G,Martinez A

更新日期：2007-08-01 00:00:00

abstract：:Hypoxia is encountered frequently by Candida albicans during systemic infection of the human host. We tested if hypoxia allows biofilm formation by C. albicans, which is a major cause of perseverance and antifungal resistance in C. albicans infections. Using an in vitro biofilm system, we unexpectedly discovered that ...

journal_title：Applied and environmental microbiology

authors： Stichternoth C,Ernst JF

更新日期：2009-06-01 00:00:00

abstract：:The isoamylase gene from Flavobacterium odoratum KU was cloned into and expressed in Escherichia coli JM109. The promoter of the gene was successful in E. coli, and the enzyme produced was excreted into the culture medium, depending on the amount of the enzyme expressed. The enzyme found in the culture medium showed a...

journal_title：Applied and environmental microbiology

authors： Abe J,Ushijima C,Hizukuri S

更新日期：1999-09-01 00:00:00

abstract：:Two strains of 1,1,1-trichloroethane (TCA)-degrading bacteria, TA5 and TA27, were isolated from soil and identified as Mycobacterium spp. Strains TA5 and TA27 could degrade 25 and 75 mg. liter of TCA(-1) cometabolically in the presence of ethane as a carbon source, respectively. The compound 2,2,2-trichloroethanol was...

journal_title：Applied and environmental microbiology

authors： Yagi O,Hashimoto A,Iwasaki K,Nakajima M

更新日期：1999-10-01 00:00:00

abstract：:The siderophore synthetic system in Shewanella species is able to synthesize dozens of macrocyclic siderophores in vitro with synthetic precursors. In vivo, however, although three siderophores are produced naturally in Shewanella algae B516, which carries a lysine decarboxylase (AvbA) specific for siderophore synthes...

journal_title：Applied and environmental microbiology

authors： Wang S,Liang H,Liu L,Jiang X,Wu S,Gao H

更新日期：2020-03-18 00:00:00

abstract：:Disruption of the aflatoxin biosynthesis cluster gene aflY (hypA) gave Aspergillus parasiticus transformants that accumulated versicolorin A. This gene is predicted to encode the Baeyer-Villiger oxidase necessary for formation of the xanthone ring of the aflatoxin precursor demethylsterigmatocystin. ...

journal_title：Applied and environmental microbiology

authors： Ehrlich KC,Montalbano B,Boué SM,Bhatnagar D

更新日期：2005-12-01 00:00:00

abstract：:Gibberellins (GAs) are tetracyclic diterpenoid phytohormones that were first identified as secondary metabolites of the fungus Fusarium fujikuroi (teleomorph, Gibberella fujikuroi). GAs were also found in the cassava pathogen Sphaceloma manihoticola, but the spectrum of GAs differed from that in F. fujikuroi. In contr...

journal_title：Applied and environmental microbiology

authors： Bömke C,Rojas MC,Gong F,Hedden P,Tudzynski B

更新日期：2008-09-01 00:00:00

abstract：:DNA extraction procedures and PCR conditions to detect Vibrio vulnificus cells naturally occurring in oysters were developed. In addition, PCR amplification of V. vulnificus from oysters seeded with biotype 1 cells was demonstrated. By the methods described, V. vulnificus cells on a medium (colistin-polymyxin B-cellob...

journal_title：Applied and environmental microbiology

authors： Coleman SS,Melanson DM,Biosca EG,Oliver JD

更新日期：1996-04-01 00:00:00

abstract：:Listeria monocytogenes can cause the serious infection listeriosis, which despite antibiotic treatment has a high mortality. Understanding the response of L. monocytogenes to antibiotic exposure is therefore important to ensure treatment success. Some bacteria survive antibiotic treatment by formation of persisters, w...

journal_title：Applied and environmental microbiology

authors： Knudsen GM,Ng Y,Gram L

更新日期：2013-12-01 00:00:00

abstract：:Mongolian gerbils were used as an animal model to excyst and host Giardia spp. isolated from meadow voles, dogs, beavers, and humans. Both cysts and trophozoites were used to establish infections. Gerbils were infected with Giardia duodenalis from beaver, dog, and human sources, and the trophozoites were extracted and...

journal_title：Applied and environmental microbiology

authors： Wallis PM,Wallis HM

更新日期：1986-03-01 00:00:00

abstract：:Phage therapy is being reexamined as a strategy for bacterial control in medical and other environments. As microorganisms often live in mixed populations, we examined the effect of Escherichia coli bacteriophage λW60 and Pseudomonas aeruginosa bacteriophage PB-1 infection on the viability of monoculture and mixed-spe...

journal_title：Applied and environmental microbiology

authors： Kay MK,Erwin TC,McLean RJ,Aron GM

更新日期：2011-02-01 00:00:00

abstract：:A basal (BC) medium devoid of added carbohydrates, a complete (CC) medium containing nine carbohydrates were developed for enumerating rumen bacteria. The colony counts on the BC medium were 85 to 100% of those obtained on the CC medium. These colonies were pinpoint size (less than or equal to mm in diameter) but incr...

journal_title：Applied and environmental microbiology

authors： Leedle JA,Hespell RB

更新日期：1980-04-01 00:00:00

abstract：:Acid pretreatment is commonly used to release pentoses from the hemicellulose fraction of cellulosic biomass for bioconversion. The predominant pentose in the hemicellulose fraction of hardwoods and crop residues is xylose in the polysaccharide methylglucuronoxylan, in which as many as one in six of the beta-1,4-linke...

journal_title：Applied and environmental microbiology

authors： Bi C,Rice JD,Preston JF

更新日期：2009-01-01 00:00:00

abstract：:Cells in desiccated Salmonella enterica serovar Typhimurium rdar (red, dry, and rough) morphotype colonies were examined for culturability and infectivity after 30 months. Culturability decreased only 10-fold; however, cells were underrepresented on Salmonella selective media containing bile salts. These cells were mi...

journal_title：Applied and environmental microbiology

authors： Apel D,White AP,Grassl GA,Finlay BB,Surette MG

更新日期：2009-07-01 00:00:00

abstract：:The bifidobacterial and lactobacillus populations of fecal samples collected from 10 human subjects were studied. The numbers of bifidobacteria were similar in the fecal samples of all of the subjects, but lactobacillus numbers varied, even between samples collected from the same individual. Analysis of the compositio...

journal_title：Applied and environmental microbiology

authors： Kimura K,McCartney AL,McConnell MA,Tannock GW

更新日期：1997-09-01 00:00:00

abstract：:Phylogenetic and "fingerprinting" analyses of the 16S rRNA genes of prokaryotes have been a mainstay of microbial ecology during the last two decades. However, many methods and results from studies that rely on the 16S rRNA gene for detection and quantification of specific microbial taxa have seemingly received only c...

journal_title：Applied and environmental microbiology

authors： Morales SE,Holben WE

更新日期：2009-05-01 00:00:00

abstract：:Four sourdoughs (A to D) were produced under practical conditions by using a starter mixture of three commercially available sourdough starters and a baker's yeast constitutively containing various species of lactic acid bacteria (LAB). The sourdoughs were continuously propagated until the composition of the LAB flora...

journal_title：Applied and environmental microbiology

authors： Meroth CB,Walter J,Hertel C,Brandt MJ,Hammes WP

更新日期：2003-01-01 00:00:00

abstract：:The leaf colonization strategies of two bacterial strains were investigated. The foliar pathogen Pseudomonas syringae pv. syringae strain B728a and the nonpathogen Pantoea agglomerans strain BRT98 were marked with a green fluorescent protein, and surface (epiphytic) and subsurface (endophytic) sites of bean and maize ...

journal_title：Applied and environmental microbiology

authors： Sabaratnam S,Beattie GA

更新日期：2003-02-01 00:00:00

abstract：:The environmental transport of Cryptosporidium spp. through combined sewer overflow (CSO) and the occurrence of several emerging human-pathogenic Cryptosporidium species in developing countries remain unclear. In this study, we collected 40 CSO samples and 40 raw wastewater samples from Shanghai, China, and examined t...

journal_title：Applied and environmental microbiology

authors： Huang C,Hu Y,Wang L,Wang Y,Li N,Guo Y,Feng Y,Xiao L

更新日期：2017-08-01 00:00:00

abstract：:Listeria monocytogenes is a food-borne pathogen with the ability to grow under conditions of high osmolarity. In a previous study, we reported the identification of 12 proteins showing high induction after salt stress. One of these proteins is highly similar to the general stress protein Ctc of Bacillus subtilis. In t...

journal_title：Applied and environmental microbiology

authors： Gardan R,Duché O,Leroy-Sétrin S,Labadie J,European Listeria Genome Consortium.

更新日期：2003-01-01 00:00:00

abstract：:Aminobacter sp. strain MSH1 grows on and mineralizes the groundwater micropollutant 2,6-dichlorobenzamide (BAM) and is of interest for BAM removal in drinking water treatment plants (DWTPs). The BAM-catabolic genes in MSH1 are located on plasmid pBAM1, carrying bbdA, which encodes the conversion of BAM to 2,6-dichloro...

journal_title：Applied and environmental microbiology

authors： Horemans B,Raes B,Brocatus H,T'Syen J,Rombouts C,Vanhaecke L,Hofkens J,Springael D

更新日期：2017-05-17 00:00:00