Differential carbohydrate media and anaerobic replica plating techniques in delineating carbohydrate-utilizing subgroups in rumen bacterial populations.

abstract：:More than 40 bacterial strains belonging to the cosmopolitan Polynucleobacter necessarius cluster (Betaproteobacteria) were isolated from a broad spectrum of freshwater habitats located in three climatic zones. Sequences affiliated with the freshwater P. necessarius cluster are among the most frequently detected in st...

journal_title：Applied and environmental microbiology

authors： Hahn MW

更新日期：2003-09-01 00:00:00

abstract：:Biphasic systems can overcome the problem of low productivity in conventional media and have been exploited for biocatalysis. Solvent-tolerant microorganisms are useful in biotransformation with whole cells in biphasic reactions. A solvent-tolerant desulfurizing bacterium, Pseudomonas putida A4, was constructed by int...

journal_title：Applied and environmental microbiology

authors： Tao F,Yu B,Xu P,Ma CQ

更新日期：2006-07-01 00:00:00

abstract：:Swimming in ocean water, including ocean water at beaches not impacted by known point sources of pollution, is an increasing health concern. This study was an initial evaluation of the presence of indicator microbes and pathogens and the association among the indicator microbes, pathogens, and environmental conditions...

journal_title：Applied and environmental microbiology

authors： Abdelzaher AM,Wright ME,Ortega C,Solo-Gabriele HM,Miller G,Elmir S,Newman X,Shih P,Bonilla JA,Bonilla TD,Palmer CJ,Scott T,Lukasik J,Harwood VJ,McQuaig S,Sinigalliano C,Gidley M,Plano LR,Zhu X,Wang JD,Fleming LE

更新日期：2010-02-01 00:00:00

abstract：:We describe here a new enzyme-coupled assay for the quantitation of d-xylose using readily available enzymes that allows kinetic evaluation of hemicellulolytic enzymes using natural xylooligosaccharide substrates. Hydrogen peroxide is generated as an intermediary analyte, which allows flexibility in the choice of the ...

journal_title：Applied and environmental microbiology

authors： Wagschal K,Franqui-Espiet D,Lee CC,Robertson GH,Wong DW

更新日期：2005-09-01 00:00:00

abstract：:The epiphytic fitness of Salmonella enterica was assessed on cilantro plants by using a strain of S. enterica serovar Thompson that was linked to an outbreak resulting from cilantro. Salmonella serovar Thompson had the ability to colonize the surface of cilantro leaves, where it was detected by confocal laser scanning...

journal_title：Applied and environmental microbiology

authors： Brandl MT,Mandrell RE

更新日期：2002-07-01 00:00:00

abstract：:Salmonella enterica serovar Typhimurium was grown at salt concentrations ranging from 0.5 to 7.5% in minimal medium with and without added osmoprotectant and in a rich medium. In minimal medium, the cells showed an initial decline period, and consequently the definition of the lag time of the resultant log count curve...

journal_title：Applied and environmental microbiology

authors： Zhou K,George SM,Métris A,Li PL,Baranyi J

更新日期：2011-03-01 00:00:00

abstract：:To examine the relationship between activated-sludge bulking and levels of specific filamentous bacteria, we developed a statistics-based quantification method for estimating the biomass levels of specific filaments using 16S rRNA-targeted fluorescent in situ hybridization (FISH) probes. The results of quantitative FI...

journal_title：Applied and environmental microbiology

authors： Liao J,Lou I,de los Reyes FL 3rd

更新日期：2004-04-01 00:00:00

abstract：:DNA extraction procedures and PCR conditions to detect Vibrio vulnificus cells naturally occurring in oysters were developed. In addition, PCR amplification of V. vulnificus from oysters seeded with biotype 1 cells was demonstrated. By the methods described, V. vulnificus cells on a medium (colistin-polymyxin B-cellob...

journal_title：Applied and environmental microbiology

authors： Coleman SS,Melanson DM,Biosca EG,Oliver JD

更新日期：1996-04-01 00:00:00

abstract：:Chlortetracycline and the macrolide tylosin were identified as commonly used antimicrobials for growth promotion and prophylaxis in swine production. Resistance to these antimicrobials was measured throughout the waste treatment processes at five swine farms by culture-based and molecular methods. Conventional farm sa...

journal_title：Applied and environmental microbiology

authors： Jindal A,Kocherginskaya S,Mehboob A,Robert M,Mackie RI,Raskin L,Zilles JL

更新日期：2006-12-01 00:00:00

abstract：:Bacterial isolates from frogs were phenotypically identified as Ochrobactrum anthropi, but 16S rRNA sequencing showed up to 100% identity with Brucella inopinata. Further analysis of recA, omp2a, omp2b, bcsp31, and IS711 and multilocus sequence analysis (MLSA) verified a close relationship with Brucella, suggesting th...

journal_title：Applied and environmental microbiology

authors： Eisenberg T,Hamann HP,Kaim U,Schlez K,Seeger H,Schauerte N,Melzer F,Tomaso H,Scholz HC,Koylass MS,Whatmore AM,Zschöck M

更新日期：2012-05-01 00:00:00

abstract：:The aim of this work was to identify genes in Lactococcus lactis subsp. lactis IL1403 and Lactococcus lactis subsp. cremoris Wg2 important for adsorption of the 936-species phages bIL170 and phi 645, respectively. Random insertional mutagenesis of the two L. lactis strains was carried out with the vector pGh9:ISS1, an...

journal_title：Applied and environmental microbiology

authors： Dupont K,Janzen T,Vogensen FK,Josephsen J,Stuer-Lauridsen B

更新日期：2004-10-01 00:00:00

abstract：:Bacillus subtilis RB14, which showed antibiotic activities against several phytopathogens in vitro by producing the antibiotics iturin A and surfactin, was subjected to a pot test to investigate its ability to suppress damping-off of tomato seedlings caused by Rhizoctonia solani. To facilitate recovery from soil, B. s...

journal_title：Applied and environmental microbiology

authors： Asaka O,Shoda M

更新日期：1996-11-01 00:00:00

abstract：:Pulsed-field gel electrophoresis established the linkage between recalled chocolate milk and a multistate invasive listeriosis outbreak during a four-product recall period. Listeria monocytogenes isolates from four hospitalized patients and an environmental dairy sample displayed AscI restriction endonuclease digestio...

journal_title：Applied and environmental microbiology

authors： Proctor ME,Brosch R,Mellen JW,Garrett LA,Kaspar CW,Luchansky JB

更新日期：1995-08-01 00:00:00

abstract：:From Bradyrhizobium japonicum highly reiterated sequence-possessing (HRS) strains indigenous to Niigata and Tokachi in Japan with high copy numbers of the repeated sequences RSalpha and RSbeta (K. Minamisawa, T. Isawa, Y. Nakatsuka, and N. Ichikawa, Appl. Environ. Microbiol. 64:1845-1851, 1998), several insertion sequ...

journal_title：Applied and environmental microbiology

authors： Isawa T,Sameshima R,Mitsui H,Minamisawa K

更新日期：1999-08-01 00:00:00

abstract：:Restriction endonuclease fragment patterns of the deoxyribonucleic acid genomes of Autographa californica nuclear polyhedrosis virus (multiply embedded type) and Trichoplusia ni nuclear polyhedrosis virus (multiply embedded type) demonstrate that the two viruses are distinct but closely related variants. ...

journal_title：Applied and environmental microbiology

authors： Miller LK,Dawes KP

更新日期：1978-06-01 00:00:00

abstract：:Novel strains of obligately chemolithoautotrophic, sulfur-oxidizing bacteria have been isolated from various depths of Lake Fryxell, Antarctica. Physiological, morphological, and phylogenetic analyses showed these strains to be related to mesophilic Thiobacillus species, such as T. thioparus. However, the psychrotoler...

journal_title：Applied and environmental microbiology

authors： Sattley WM,Madigan MT

更新日期：2006-08-01 00:00:00

abstract：:A field evaluation of double violet agar for the isolation and presumptive identification of Klebsiella pneumoniae from water has been performed. Water from the North Oconee River, Clarke County, Ga., was cultured for presence of klebsiellae using the membrane filter technique. Colonies were presumptively identified a...

journal_title：Applied and environmental microbiology

authors： Campbell LM,Roth IL,Klein RD

更新日期：1976-02-01 00:00:00

abstract：:Information on the interaction between mixed populations in the rumen and plant phenolics is required to fully elucidate the limitations of phenolic compounds on forage digestibility. The objective of this study was to examine the degradation of Italian ryegrass (Lolium multiflorum L.) hay incubated with mixed ruminal...

journal_title：Applied and environmental microbiology

authors： Theodorou MK,Gascoyne DJ,Akin DE,Hartley RD

更新日期：1987-05-01 00:00:00

abstract：:Estimations of the bacterial content of air can be more easily made now than a decade ago, with colony formation the method of choice for enumeration of airborne bacteria. However, plate counts are subject to error because bacteria exposed to the air may remain viable yet lose the ability to form colonies, i.e., they ...

journal_title：Applied and environmental microbiology

authors： Heidelberg JF,Shahamat M,Levin M,Rahman I,Stelma G,Grim C,Colwell RR

更新日期：1997-09-01 00:00:00

abstract：:Three 16S rRNA hybridization probes were developed and tested for genus-specific detection of Bifidobacterium species in the human fecal flora. Variable regions V2, V4, and V8 of the 16S rRNA contained sequences unique to this genus and proved applicable as target sites for oligodeoxynucleotide probes. Determination o...

journal_title：Applied and environmental microbiology

authors： Langendijk PS,Schut F,Jansen GJ,Raangs GC,Kamphuis GR,Wilkinson MH,Welling GW

更新日期：1995-08-01 00:00:00

abstract：:The spice oil components eugenol and cinnamaldehyde possess activity against both gram-positive and gram-negative bacteria, but the mechanisms of action remain obscure. In broth media at 20 degrees C, 5 mM eugenol or 30 mM cinnamaldehyde was bactericidal (>1-log reduction in the number of CFU per milliliter in 1 h) to...

journal_title：Applied and environmental microbiology

authors： Gill AO,Holley RA

更新日期：2004-10-01 00:00:00

abstract：:Hybridization of crushed Lotus pedunculatus root nodules with P-labeled total genomic DNA probes was used to identify Rhizobium loti and Bradyrhizobium sp. (Lotus rhizobia). Probes always hybridized with homologous target DNA and frequently with DNAs of other strains from the same genus. Intergeneric hybridization did...

journal_title：Applied and environmental microbiology

authors： Cooper JE,Bjourson AJ,Thompson JK

更新日期：1987-07-01 00:00:00

abstract：:Characterization by partial 16S rRNA gene sequencing, ribotyping, and green fluorescent protein-based nisin bioassay revealed that 6 of 20 human milk samples contained nisin-producing Lactococcus lactis bacteria. This suggests that the history of humans consuming nisin is older than the tradition of consuming fermente...

journal_title：Applied and environmental microbiology

authors： Beasley SS,Saris PE

更新日期：2004-08-01 00:00:00

abstract：:Two Baeyer-Villiger monooxygenases (BVMOs), designated BoBVMO and AmBVMO, were discovered from Bradyrhizobium oligotrophicum and Aeromicrobium marinum, respectively. Both monooxygenases displayed novel features for catalyzing the asymmetric sulfoxidation of bulky and pharmaceutically relevant thioethers. Evolutionary ...

journal_title：Applied and environmental microbiology

authors： Zhang Y,Liu F,Xu N,Wu YQ,Zheng YC,Zhao Q,Lin G,Yu HL,Xu JH

更新日期：2018-07-02 00:00:00

abstract：UNLABELLED:Lactobacillus rhamnosus GG is a lactic acid bacterium widely marketed by the food industry. Its genomic analysis led to the identification of a gene cluster encoding mucus-binding SpaCBA pili, which is located in a genomic island enriched in insertion sequence (IS) elements. In the present study, we analyzed...

journal_title：Applied and environmental microbiology

authors： Douillard FP,Ribbera A,Xiao K,Ritari J,Rasinkangas P,Paulin L,Palva A,Hao Y,de Vos WM

更新日期：2016-06-13 00:00:00

abstract：:Fifteen Bacillus thuringiensis strains representing 13 serotypes were screened with five oligodeoxyribonucleotide probes specific for certain regions of two published sequences and one unpublished sequence of B. thuringiensis delta-endotoxin genes. Of the 15 cultures, 14 hybridized with at least one probe; the B. thur...

journal_title：Applied and environmental microbiology

authors： Prefontaine G,Fast P,Lau PC,Hefford MA,Hanna Z,Brousseau R

更新日期：1987-12-01 00:00:00

abstract：:We investigated the genetic diversity of symbiotic bacteria associated with two newly discovered species of Osedax from Monterey Canyon, CA, at 1,017-m (Osedax Monterey Bay sp. 3 "rosy" [Osedax sp. MB3]) and 381-m (Osedax Monterey Bay sp. 4 "yellow collar") depths. Quantitative PCR and clone libraries of 16S rRNA gene...

journal_title：Applied and environmental microbiology

authors： Goffredi SK,Johnson SB,Vrijenhoek RC

更新日期：2007-04-01 00:00:00

abstract：:This study investigated the effects of long-term soil fertilization on the composition and potential for phosphorus (P) and nitrogen (N) cycling of bacterial communities associated with hyphae of the P-solubilizing fungus Penicillium canescens Using a baiting approach, hyphosphere bacterial communities were recovered ...

journal_title：Applied and environmental microbiology

authors： Zhang Y,Hao X,Garcia-Lemos AM,Nunes I,Nicolaisen MH,Nybroe O

更新日期：2020-05-05 00:00:00

abstract：:Methyl fluoride is frequently used to specifically inhibit acetoclastic methanogenesis, thus allowing determination of the relative contribution of acetate versus H2/CO2 to total CH4 production in natural environments. However, the effect of the inhibitor on growth of the target archaeal population has not yet been st...

journal_title：Applied and environmental microbiology

authors： Penning H,Conrad R

更新日期：2006-01-01 00:00:00

abstract：:With an initial microbial level of ca. 10 microorganisms per g of Ivory Coast cacao beans, 5 kGy of gamma radiation under an atmosphere of air reduced the microflora per g by 2.49 and 3.03 logs at temperatures of 35 and 50 degrees C, respectively. Bahia cacao beans were artificially contaminated with dried spores of A...

journal_title：Applied and environmental microbiology

authors： Restaino L,Myron JJ,Lenovich LM,Bills S,Tscherneff K

更新日期：1984-04-01 00:00:00