Use of oligonucleotide probes to study the relatedness of delta-endotoxin genes among Bacillus thuringiensis subspecies and strains.

abstract：:Reverse transcription-PCR analysis of drinking water in the homes of 56 children suffering from rotaviral gastroenteritis has shown the presence of the rotavirus genome in four samples. These strains were different from human rotaviruses detected in the children's feces, as determined by sequencing of the VP7-amplifie...

journal_title：Applied and environmental microbiology

authors： Gratacap-Cavallier B,Genoulaz O,Brengel-Pesce K,Soule H,Innocenti-Francillard P,Bost M,Gofti L,Zmirou D,Seigneurin JM

更新日期：2000-06-01 00:00:00

abstract：:The Cry2Aa3 gene was introduced into asporogenic Bacillus thuringiensis, and the synthesized protoxin killed Bombyx mori and Lymantria dispar larvae. Chymotrypsin hydrolyzed the linkages between 49Tyr/Val50 and 145Lys/Ser146 in the protoxin, and 50- and 58-kDa fragments were generated, respectively. Both peptides kill...

journal_title：Applied and environmental microbiology

authors： Ohsawa M,Tanaka M,Moriyama K,Shimazu M,Asano S,Miyamoto K,Haginoya K,Mitsui T,Kouya T,Taniguchi M,Hori H

更新日期：2012-07-01 00:00:00

abstract：:Bacillus subtilis 5230 spores were lyophilized in 0.067 M phosphate buffer and stored at 2 to 8 degrees C for 9 to 27 months. The lyophilized spores were reconstituted with buffer or 0.9% saline, and the heat resistance was determined in a thermoresistometer. Lyophilization had no effect on the heat resistance of the ...

journal_title：Applied and environmental microbiology

authors： Odlaug TE,Caputo RA,Graham GS

更新日期：1981-06-01 00:00:00

abstract：:Light microscopy and a radioassay detected no significant difference in adherence of Kanagawa-positive and Kanagawa-negative strains of Vibrio parahaemolyticus to human epithelial cell lines. ...

journal_title：Applied and environmental microbiology

authors： Gingras SP,Howard LV

更新日期：1980-02-01 00:00:00

abstract：:Characterization of the mle locus harboring the malolactic enzyme gene mleA and malate permease gene mleP from Leuconostoc oenos was completed in this study by mRNA analysis. Northern (RNA) blot experiments revealed a 2.6-kb transcript, suggesting an operon structure harboring mleA and mleP genes. Primer extension ana...

journal_title：Applied and environmental microbiology

authors： Labarre C,Diviès C,Guzzo J

更新日期：1996-12-01 00:00:00

abstract：:The role of Escherichia coli as a pathogen has been the focus of considerable study, while much less is known about it as a commensal and how it adapts to and colonizes different environmental niches within the mammalian gut. In this study, we characterize Escherichia coli organisms (n = 146) isolated from different r...

journal_title：Applied and environmental microbiology

authors： Abraham S,Gordon DM,Chin J,Brouwers HJ,Njuguna P,Groves MD,Zhang R,Chapman TA

更新日期：2012-10-01 00:00:00

abstract：:The N-acetylglucosaminidase LytB of Streptococcus pneumoniae is involved in nasopharyngeal colonization and is responsible for cell separation at the end of cell division; thus, ΔlytB mutants form long chains of cells. This paper reports the construction and properties of a defective pneumococcal mutant producing an i...

journal_title：Applied and environmental microbiology

authors： Domenech M,García E

更新日期：2020-05-05 00:00:00

abstract：UNLABELLED:Copper sulfate (CuSO4) has been widely used as an algicide to control harmful cyanobacterial blooms (CyanoHABs) in freshwater lakes. However, there are increasing concerns about this application, due mainly to the general toxicity of CuSO4 to other aquatic species and its long-term persistence in the environ...

journal_title：Applied and environmental microbiology

authors： Zhang BH,Ding ZG,Li HQ,Mou XZ,Zhang YQ,Yang JY,Zhou EM,Li WJ

更新日期：2016-08-15 00:00:00

abstract：:Genomic probes were used to investigate hepatitis A virus (HAV) and enterovirus RNAs in two types of shellfish from natural beds (Atlantic coast, France). After elution concentration, nucleic acid extracted by proteinase K and purified by phenol-chloroform and ethanol precipitation was assayed by dot blot hybridizatio...

journal_title：Applied and environmental microbiology

authors： Le Guyader F,Apaire-Marchais V,Brillet J,Billaudel S

更新日期：1993-11-01 00:00:00

abstract：:The internal pH of Clostridium acetobutylicum was determined at various stages during the growth of the organism. Even in the presence of significant quantities of acetic, butyric, and lactic acids, an internal pH of 6.2 was maintained. Experiments using N,N'-dicyclohexylcarbodiimide indicated that a functioning H+-AT...

journal_title：Applied and environmental microbiology

authors： Bowles LK,Ellefson WL

更新日期：1985-11-01 00:00:00

abstract：:Streptococcus pyogenes is a major cause of necrotizing fasciitis, a life-threatening subcutaneous soft-tissue infection. At the host infection site, the local environment and interactions between the host and bacteria have effects on bacterial gene expression profiles, while the gene expression pattern of S. pyogenes ...

journal_title：Applied and environmental microbiology

authors： Hirose Y,Yamaguchi M,Okuzaki D,Motooka D,Hamamoto H,Hanada T,Sumitomo T,Nakata M,Kawabata S

更新日期：2019-10-16 00:00:00

abstract：:The crenarchaeon Sulfolobus acidocaldarius has been described to synthesize trehalose via the maltooligosyltrehalose synthase (TreY) and maltooligosyltrehalose trehalohydrolase (TreZ) pathway, and the trehalose glycosyltransferring synthase (TreT) pathway has been predicted. Deletion mutant analysis of strains with si...

journal_title：Applied and environmental microbiology

authors： Stracke C,Meyer BH,Hagemann A,Jo E,Lee A,Albers SV,Cha J,Bräsen C,Siebers B

更新日期：2020-11-24 00:00:00

abstract：:Campylobacter jejuni and Campylobacter coli are naturally competent, but limited information exists on the impact of environmental conditions on transformation. In this study, we investigated the impact of temperature and microaerobic versus aerobic atmosphere on transformation of C. coli to erythromycin and nalidixic...

journal_title：Applied and environmental microbiology

authors： Kim JS,Kim JW,Kathariou S

更新日期：2008-10-01 00:00:00

abstract：:"Caldocellum saccharolyticum" is an obligatory anaerobic thermophilic bacterium. A gene from this organism, designated celB, has been cloned in Escherichia coli as part of a bacteriophage lambda gene library. This gene produces a thermostable cellulase that shows both endoglucanase and exoglucanase activities on test ...

journal_title：Applied and environmental microbiology

authors： Saul DJ,Williams LC,Grayling RA,Chamley LW,Love DR,Bergquist PL

更新日期：1990-10-01 00:00:00

abstract：:We report the characterization of the ccpA gene of Lactobacillus plantarum, coding for catabolite control protein A. The gene is linked to the pepQ gene, encoding a proline peptidase, in the order ccpA-pepQ, with the two genes transcribed in tandem from the same strand as distinct transcriptional units. Two ccpA trans...

journal_title：Applied and environmental microbiology

authors： Muscariello L,Marasco R,De Felice M,Sacco M

更新日期：2001-07-01 00:00:00

abstract：:Pigment production by the fungus Monascus sp. was studied to determine why Monascus sp. provides more pigment in solid culture than in submerged culture. Adding a sterilized nonionic polymeric adsorbent resin directly to the growing submerged culture did not enhance the pigment production, thus indicating that pigment...

journal_title：Applied and environmental microbiology

authors： Evans PJ,Wang HY

更新日期：1984-06-01 00:00:00

abstract：:A nodule-shaped microbial mat was found subsurface in sediments of a gas seep in the anoxic Black Sea. This mat was dominated by ANME-1 archaea and consumed methane and sulfate simultaneously. We propose that such subsurface mats represent the initial stage of previously investigated microbial reefs. ...

journal_title：Applied and environmental microbiology

authors： Treude T,Knittel K,Blumenberg M,Seifert R,Boetius A

更新日期：2005-10-01 00:00:00

abstract：:We tested the sensitivity of coastal picophytoplankton exposed to natural sunlight in short-term experiments. Cell abundance and cell-specific chlorophyll fluorescence were significantly reduced in Prochlorococcus spp. but not in Synechococcus, whereas picoeukaryotes had an intermediate response. These results are the...

journal_title：Applied and environmental microbiology

authors： Sommaruga R,Hofer JS,Alonso-Sáez L,Gasol JM

更新日期：2005-04-01 00:00:00

abstract：:A census of clean room surface-associated bacterial populations was derived from the results of both the cloning and sequencing of 16S rRNA genes and DNA microarray (PhyloChip) analyses. Samples from the Lockheed Martin Aeronautics Multiple Testing Facility (LMA-MTF), the Kennedy Space Center Payload Hazard and Servic...

journal_title：Applied and environmental microbiology

authors： La Duc MT,Osman S,Vaishampayan P,Piceno Y,Andersen G,Spry JA,Venkateswaran K

更新日期：2009-10-01 00:00:00

abstract：:The natural products of both eremofortin C (EC) and PR toxin are secondary metabolites of Penicillium roqueforti. Because the chemical structures of EC and PR toxin are closely related to each other and differ only by a hydroxyl functional group in EC and an aldehyde functional group in PR toxin at the C-12 position, ...

journal_title：Applied and environmental microbiology

authors： Li SY,Chang SC,Wei RD

更新日期：1985-09-01 00:00:00

abstract：:A novel xylanase (xylanase IV) which produces xylotetraose as the only low-molecular-weight oligosaccharide from oat spelt xylan was isolated from the culture medium of Aeromonas caviae ME-1. By sodium dodecyl sulfate-polyacrylamide gel electrophoresis, the xylanase IV molecular weight was 41,000. Xylanase IV catalyze...

journal_title：Applied and environmental microbiology

authors： Kubata BK,Takamizawa K,Kawai K,Suzuki T,Horitsu H

更新日期：1995-04-01 00:00:00

abstract：:Four membrane filter methods for the enumeration of fecal coliforms were compared for accuracy, specificity, and recovery. Water samples were taken several times from 13 marine, 1 estuarine, and 4 freshwater sites around Puerto Rico, from pristine waters and waters receiving treated and untreated sewage and effluent f...

journal_title：Applied and environmental microbiology

authors： Santiago-Mercado J,Hazen TC

更新日期：1987-12-01 00:00:00

abstract：:Gene clusters for biosynthesis of the fungal polyketides hypothemycin and radicicol from Hypomyces subiculosus and Pochonia chlamydosporia, respectively, were sequenced. Both clusters encode a reducing polyketide synthase (PKS) and a nonreducing PKS like those in the zearalenone cluster of Gibberella zeae, plus enzyme...

journal_title：Applied and environmental microbiology

authors： Reeves CD,Hu Z,Reid R,Kealey JT

更新日期：2008-08-01 00:00:00

abstract：:The presence of neurotoxic species within the genus Alexandrium along the U.S. coastline has raised concern of potential poisoning through the consumption of contaminated seafood. Paralytic shellfish toxins (PSTs) detected in shellfish provide evidence that these harmful events have increased in frequency and severity...

journal_title：Applied and environmental microbiology

authors： Garneau MÈ,Schnetzer A,Countway PD,Jones AC,Seubert EL,Caron DA

更新日期：2011-11-01 00:00:00

abstract：:The effect of condensed tannins from birdsfoot trefoil (Lotus corniculatus L.) on the cellulolytic rumen bacterium Fibrobacter succinogenes S85 was examined. Condensed tannins inhibited endoglucanase activity in the extracellular culture fluid, at concentrations as low as 25 mug ml. In contrast, cell-associated endogl...

journal_title：Applied and environmental microbiology

authors： Bae HD,McAllister TA,Yanke J,Cheng KJ,Muir AD

更新日期：1993-07-01 00:00:00

abstract：:Among the Frankia strains capable of establishing a nitrogen-fixing symbiosis with actinorrhizal plants, in planta sporangial formation is not universal and has led to the distinction between spore-positive (Sp+) and spore-negative (Sp-) nodules. Numerous Frankia strains have been isolated in pure culture from Sp+ nod...

journal_title：Applied and environmental microbiology

authors： Simonet P,Bosco M,Chapelon C,Moiroud A,Normand P

更新日期：1994-04-01 00:00:00

abstract：:Two strains of small spirochetes utilizing pectin as sole source of energy for growth were isolated from the rumen of cattle and partially characterized. The pectinolytic enzyme produced by one of the organisms was classified as a poly-1,4-alpha-D-galacturonide lyase (EC 4.2.2.2). A pectinesterase (EC 3.1.1.11) was al...

journal_title：Applied and environmental microbiology

authors： Ziołecki A,Wojciechowicz M

更新日期：1980-04-01 00:00:00

abstract：:The symbiotic association between the roseobacter Silicibacter sp. strain TM1040 and the dinoflagellate Pfiesteria piscicida involves bacterial chemotaxis to dinoflagellate-produced dimethylsulfoniopropionate (DMSP), DMSP demethylation, and ultimately a biofilm on the surface of the host. Biofilm formation is coincide...

journal_title：Applied and environmental microbiology

authors： Geng H,Bruhn JB,Nielsen KF,Gram L,Belas R

更新日期：2008-03-01 00:00:00

abstract：:Suspensions of Nitrosomonas europaea catalyzed the oxidation of the commercial nitrification inhibitor nitrapyrin [2-chloro-6-(trichloromethyl)-pyridine]. Rapid oxidation of nitrapyrin (at a concentration of 10 muM) required the concomitant oxidation of ammonia, hydroxylamine, or hydrazine. The turnover rate was highe...

journal_title：Applied and environmental microbiology

authors： Vannelli T,Hooper AB

更新日期：1992-07-01 00:00:00

abstract：:Although many Lactobacillus strains used as probiotics are believed to modulate host immune responses, the molecular natures of the components of such probiotic microorganisms directly involved in immune modulation process are largely unknown. We aimed to assess the function of polysaccharide moiety of the cell wall o...

journal_title：Applied and environmental microbiology

authors： Yasuda E,Serata M,Sako T

更新日期：2008-08-01 00:00:00