Abstract:
:Three 16S rRNA hybridization probes were developed and tested for genus-specific detection of Bifidobacterium species in the human fecal flora. Variable regions V2, V4, and V8 of the 16S rRNA contained sequences unique to this genus and proved applicable as target sites for oligodeoxynucleotide probes. Determination of the genus specificity of the oligonucleotides was performed by whole-cell hybridization with fluorescein isothiocyanate-labelled probes. To this end, cells were fixed on glass slides, hybridized with the probes, and monitored by videomicroscopy. In combination with image analysis, this allowed quantification of the fluorescence per cell and objective evaluation of hybridization experiments. One of the probes developed was used to determine the population of Bifidobacterium spp. in human fecal samples. A comparison was made with results obtained by cultural methods for enumeration. Since both methods gave similar population estimates, it was concluded that all bifidobacteria in feces were culturable. However, since the total culturable counts were only a fraction of the total microscopic counts, the contribution of bifidobacteria to the total intestinal microflora was overestimated by almost 10-fold when cultural methods were used as the sole method for enumeration.
journal_name
Appl Environ Microbioljournal_title
Applied and environmental microbiologyauthors
Langendijk PS,Schut F,Jansen GJ,Raangs GC,Kamphuis GR,Wilkinson MH,Welling GWdoi
10.1128/AEM.61.8.3069-3075.1995subject
Has Abstractpub_date
1995-08-01 00:00:00pages
3069-75issue
8eissn
0099-2240issn
1098-5336journal_volume
61pub_type
杂志文章abstract::The conjugal transfer of R-plasmids is known to occur at temperatures above 22 degrees C. We found that R1drd-19 is transferable below 22 degrees C, and we discuss this finding in the context of plasmid transfer in environmental waters. ...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.42.5.789-791.1981
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pub_type: 杂志文章
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更新日期:1978-08-01 00:00:00
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pub_type: 杂志文章
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journal_title:Applied and environmental microbiology
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doi:10.1128/AEM.33.2.262-268.1977
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pub_type: 杂志文章
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更新日期:2004-07-01 00:00:00
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journal_title:Applied and environmental microbiology
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doi:10.1128/AEM.62.4.1238-1241.1996
更新日期:1996-04-01 00:00:00
abstract::The seasonal variations in community structure and cell morphology of pelagic procaryotes from a high mountain lake (Gossenkollesee, Austria) were studied by in situ hybridization with rRNA-targeted fluorescently labeled oligonucleotide probes (FISH) and image-analyzed microscopy. Compositional changes and biomass flu...
journal_title:Applied and environmental microbiology
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doi:10.1128/AEM.64.11.4299-4306.1998
更新日期:1998-11-01 00:00:00
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journal_title:Applied and environmental microbiology
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更新日期:1989-06-01 00:00:00
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更新日期:1992-07-01 00:00:00
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journal_title:Applied and environmental microbiology
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journal_title:Applied and environmental microbiology
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journal_title:Applied and environmental microbiology
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journal_title:Applied and environmental microbiology
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