Abstract:
:Fourteen different broth media were autoclaved under anaerobic conditions and then exposed to atmospheric oxygen. The hydrogen peroxide and superoxide radical formation as well as the bactericidal effect of the media were studied. The rate of killing of Peptostreptococcus anaerobius VPI 4330-1 was high in media that rapidly autoxidized and accumulated hydrogen peroxide. In actinomyces broth (BBL), 50% of the cells were killed within 2 min, and in Brewer thioglycolate medium (Difco), 50% were killed within 11 min, whereas more than 50% of the cells survived for more than 2 h in Clausen medium (Oxoid), fluid thioglycolate medium (BBL), and thioglycolate medium without dextrose or indicator (Difco). Only media that contained phosphate and glucose had a tendency to accumulate hydrogen peroxide. A solution of phosphate and glucose autoxidized when it had been heated to 120 degrees C for at least 5 min and when the pH of the solution was higher than 6.5. Transitional metal ions catalyzed the autoxidation, but they were not necessary for the reaction to occur. Of the other substances heated in phosphate buffer, only alpha-hydroxycarbonyl compounds autoxidized with accumulation of hydrogen peroxide. Superoxide dismutase decreased the autoxidation rate of most of the broth media. This indicated that superoxide radicals were generated in these media.
journal_name
Appl Environ Microbioljournal_title
Applied and environmental microbiologyauthors
Carlsson J,Nyberg G,Wrethén Jdoi
10.1128/AEM.36.2.223-229.1978subject
Has Abstractpub_date
1978-08-01 00:00:00pages
223-9issue
2eissn
0099-2240issn
1098-5336journal_volume
36pub_type
杂志文章abstract::Two different Cd(2+) uptake systems were identified in Lactobacillus plantarum. One is a high-affinity, high-velocity Mn(2+) uptake system which also takes up Cd(2+) and is induced by Mn(2+) starvation. The calculated K(m) and V(max) are 0.26 microM and 3.6 micromol g of dry cell(-1) min(-1), respectively. Unlike Mn(2...
journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.65.11.4741-4745.1999
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pub_type: 杂志文章
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更新日期:2005-12-01 00:00:00
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journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.61.9.3486-3489.1995
更新日期:1995-09-01 00:00:00
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journal_title:Applied and environmental microbiology
pub_type: 杂志文章
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journal_title:Applied and environmental microbiology
pub_type: 杂志文章
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更新日期:1990-04-01 00:00:00
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pub_type: 杂志文章
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更新日期:1994-06-01 00:00:00
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journal_title:Applied and environmental microbiology
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更新日期:2005-07-01 00:00:00
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journal_title:Applied and environmental microbiology
pub_type: 杂志文章
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更新日期:2001-10-01 00:00:00
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journal_title:Applied and environmental microbiology
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doi:10.1128/AEM.64.9.3134-3139.1998
更新日期:1998-09-01 00:00:00
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journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.71.5.2592-2599.2005
更新日期:2005-05-01 00:00:00
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journal_title:Applied and environmental microbiology
pub_type: 杂志文章
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更新日期:1983-10-01 00:00:00
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journal_title:Applied and environmental microbiology
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更新日期:1982-01-01 00:00:00
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journal_title:Applied and environmental microbiology
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更新日期:1986-06-01 00:00:00
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pub_type: 杂志文章
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更新日期:2013-03-01 00:00:00
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pub_type: 杂志文章
doi:10.1128/AEM.59.9.3110-3116.1993
更新日期:1993-09-01 00:00:00
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journal_title:Applied and environmental microbiology
pub_type: 杂志文章
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更新日期:1993-11-01 00:00:00
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journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.64.1.133-137.1998
更新日期:1998-01-01 00:00:00
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journal_title:Applied and environmental microbiology
pub_type: 杂志文章
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更新日期:2012-12-01 00:00:00
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journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.39.4.709-719.1980
更新日期:1980-04-01 00:00:00
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journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.33.2.269-274.1977
更新日期:1977-02-01 00:00:00
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journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/AEM.63.11.4578-4580.1997
更新日期:1997-11-01 00:00:00
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journal_title:Applied and environmental microbiology
pub_type: 杂志文章
doi:10.1128/aem.66.5.1857-1861.2000
更新日期:2000-05-01 00:00:00